Restriction map for pF1KB4967
 (Circular) (Six-base) MAPSORT of: pF1KB4967.seq  Check: 4886  from: 1  to: 10355



pF1KB4967  10355 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   5122    5197    5883    6856    5122 
   Size:         75     686     973    8621

  Fragments arranged by size: 

                8621     973     686      75
 
AccI GT'mk_AC

Cuts at:   3521    4286    5108    6876    7393    3521 
   Size:        765     822    1768     517    6483

  Fragments arranged by size: 

                6483    1768     822     765     517
 
Acc65I G'GTAC_C

Cuts at:   7371    7371 
   Size:      10355
 
AclI AA'CG_TT

Cuts at:  10195   10195 
   Size:      10355
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    125     283     760    2290    3188    4601    6886    8180    8612 
   Size:        158     477    1530     898    1413    2285    1294     432

Cuts at:   8612    8965    9627     125 
   Size:        353     662     853

  Fragments arranged by size: 

                2285    1530    1413    1294     898     853     662     477
                 432     353     158
 
AfeI AGC'GCT

Cuts at:   5317    5317 
   Size:      10355
 
AflII C'TTAA_G

Cuts at:    832     832 
   Size:      10355
 
AflIII A'CryG_T

Cuts at:   1961    7347    7707    9507    9788    1961 
   Size:       5386     360    1800     281    2528

  Fragments arranged by size: 

                5386    2528    1800     360     281
 
AhdI GACnn_n'nnGTC

Cuts at:   6029    6029 
   Size:      10355
 
AleI CACnn'nnGTG

Cuts at:   2989    4723    2989 
   Size:       1734    8621
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   5480    6389    5480 
   Size:        909    9446
 
AlwNI CAG_nnn'CTG

Cuts at:    991    2692    2875    3279    3630    5331    6241    7660    9098 
   Size:       1701     183     404     351    1701     910    1419    1438

Cuts at:   9098     991 
   Size:       2248

  Fragments arranged by size: 

                2248    1701    1701    1438    1419     910     404     351
                 183
 
ApaI G_GGCC'C

Cuts at:   5870    5870 
   Size:      10355
 
ApaLI G'TGCA_C

Cuts at:   3654    3944    9193    3654 
   Size:        290    5249    4816

  Fragments arranged by size: 

                5249    4816     290
 
ApoI r'AATT_y

Cuts at:   1719    1805    2595    2994    5945    7215    7314    7359    9708 
   Size:         86     790     399    2951    1270      99      45    2349

Cuts at:   9708    9964    1719 
   Size:        256    2110

  Fragments arranged by size: 

                2951    2349    2110    1270     790     399     256      99
                  86      45
 
AseI AT'TA_AT

Cuts at:     20     247     290      20 
   Size:        227      43   10085

  Fragments arranged by size: 

               10085     227      43
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10355
 
AvaI C'yCGr_G

Cuts at:   4819    7375    4819 
   Size:       2556    7799
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3582    3615    5166    5199    3582 
   Size:         33    1551      33    8738

  Fragments arranged by size: 

                8738    1551      33      33
 
BamHI G'GATC_C

Cuts at:   1893    3312    3825    7380    1893 
   Size:       1419     513    3555    4868

  Fragments arranged by size: 

                4868    3555    1419     513
 
BanI G'GyrC_C

Cuts at:   4003    5441    5514    6868    7371    7785    8033    8068    4003 
   Size:       1438      73    1354     503     414     248      35    6290

  Fragments arranged by size: 

                6290    1438    1354     503     414     248      73      35
            
 
BanII G_rGCy'C

Cuts at:    787    2340    2661    3394    3449    5049    5287    5870    6595 
   Size:       1553     321     733      55    1600     238     583     725

Cuts at:   6595    6946    7369    8399     787 
   Size:        351     423    1030    2743

  Fragments arranged by size: 

                2743    1600    1553    1030     733     725     583     423
                 351     321     238      55
 
BbeI G_GCGC'C

Cuts at:   7789    8037    7789 
   Size:        248   10107
 
BbsI GAAGACnn'nnnn_

Cuts at:   1678    3122    3194    4881    1678 
   Size:       1444      72    1687    7152

  Fragments arranged by size: 

                7152    1687    1444      72
 
BbvCI CC'TCA_GC

Cuts at:   3350    5732    6110    7106    3350 
   Size:       2382     378     996    6599

  Fragments arranged by size: 

                6599    2382     996     378
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2830    2864    6054    6088    2830 
   Size:         34    3190      34    7097

  Fragments arranged by size: 

                7097    3190      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3583    5320    6334    8253    9309   10355    3583 
   Size:       1737    1014    1919    1056    1046    3583

  Fragments arranged by size: 

                3583    1919    1737    1056    1046    1014
 
BclI T'GATC_A

Cuts at:   7875    7875 
   Size:      10355
 
BfrBI ATG'CAT

Cuts at:   3870    4236    3870 
   Size:        366    9989
 
BglI GCCn_nnn'nGGC

Cuts at:    178     427    1259    2721    6432     178 
   Size:        249     832    1462    3711    4101

  Fragments arranged by size: 

                4101    3711    1462     832     249
 
BglII A'GATC_T

Cuts at:   3875    7870    9907    3875 
   Size:       3995    2037    4323

  Fragments arranged by size: 

                4323    3995    2037
 
BlpI GC'TnA_GC

Cuts at:   2660    5849    6602    7467    2660 
   Size:       3189     753     865    5548

  Fragments arranged by size: 

                5548    3189     865     753
 
Bme1580I G_kGCm'C

Cuts at:    526    1760    3658    3948    5446    5870    7980    8073    9197 
   Size:       1234    1898     290    1498     424    2110      93    1124

Cuts at:   9197     526 
   Size:       1684

  Fragments arranged by size: 

                2110    1898    1684    1498    1234    1124     424     290
                  93
 
BmrI ACTGGGnnnn_n'

Cuts at:   3207    3565    5043    6029    6969    7567    7648    7983   10127 
   Size:        358    1478     986     940     598      81     335    2144

Cuts at:  10127    3207 
   Size:       3435

  Fragments arranged by size: 

                3435    2144    1478     986     940     598     358     335
                  81
 
BmtI G_CTAG'C

Cuts at:   7241    7241 
   Size:      10355
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4269    8722    4269 
   Size:       4453    5902
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    907    1043    1103    1421    2354    2459    2519    3212    5044 
   Size:        136      60     318     933     105      60     693    1832

Cuts at:   5044    6111    6553    7065     907 
   Size:       1067     442     512    4197

  Fragments arranged by size: 

                4197    1832    1067     933     693     512     442     318
                 136     105      60      60
 
Bpu10I CC'TnA_GC

Cuts at:   1465    3350    5732    6110    7106    1465 
   Size:       1885    2382     378     996    4714

  Fragments arranged by size: 

                4714    2382    1885     996     378
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2468    4353    5762    6675    7531    8410    9124    9422    2468 
   Size:       1885    1409     913     856     879     714     298    3401

  Fragments arranged by size: 

                3401    1885    1409     913     879     856     714     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:    925    2546    6290   10059     925 
   Size:       1621    3744    3769    1221

  Fragments arranged by size: 

                3769    3744    1621    1221
 
BsaAI yAC'GTr

Cuts at:   5940    7708    5940 
   Size:       1768    8587
 
BsaBI GATnn'nnATC

Cuts at:      6     290    4952    7869    9906       6 
   Size:        284    4662    2917    2037     455

  Fragments arranged by size: 

                4662    2917    2037     455     284
 
BsaHI Gr'CG_yC

Cuts at:    181    5710    7786    8034    8736   10232     181 
   Size:       5529    2076     248     702    1496     304

  Fragments arranged by size: 

                5529    2076    1496     702     304     248
 
BsaWI w'CCGG_w

Cuts at:   3503    7544    8065    8743    9154    9301    9575    3503 
   Size:       4041     521     678     411     147     274    4283

  Fragments arranged by size: 

                4283    4041     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1394    1424    5630    5660    1394 
   Size:         30    4206      30    6089

  Fragments arranged by size: 

                6089    4206      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   2291    3196    4293    5828    2291 
   Size:        905    1097    1535    6818

  Fragments arranged by size: 

                6818    1535    1097     905
 
BseYI C'CCAG_C

Cuts at:    824    1489    2270    2567    2837    2930    3767    5714    6116 
   Size:        665     781     297     270      93     837    1947     402

Cuts at:   6116    6164    6289    6341    6545    6947    7037    7067    7780 
   Size:         48     125      52     204     402      90      30     713

Cuts at:   7780    8538    9203    9611     824 
   Size:        758     665     408    1568

  Fragments arranged by size: 

                1947    1568     837     781     758     713     665     665
                 408     402     402     297     270     204     125      93
                  90      52      48      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2801    2818    5065    5422    2801 
   Size:         17    2247     357    7734

  Fragments arranged by size: 

                7734    2247     357      17
 
BsiEI CG_ry'CG

Cuts at:    104    7897    7943    9173     104 
   Size:       7793      46    1230    1286

  Fragments arranged by size: 

                7793    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    230    1089    1407    2340    2866    3658    3948    7369    8147 
   Size:        859     318     933     526     792     290    3421     778

Cuts at:   8147    8337    9197     230 
   Size:        190     860    1388

  Fragments arranged by size: 

                3421    1388     933     860     859     792     778     526
                 318     290     190
 
BsmI GAATG_Cn'

Cuts at:    621    1007    1707    5128    5361    6355     621 
   Size:        386     700    3421     233     994    4621

  Fragments arranged by size: 

                4621    3421     994     700     386     233
 
BsmBI CGTCTCn'nnnn_

Cuts at:   5911    6651    5911 
   Size:        740    9615
 
Bsp1286I G_dGCh'C

Cuts at:    230     526     787    1089    1407    1760    2340    2661    2866 
   Size:        296     261     302     318     353     580     321     205

Cuts at:   2866    3394    3449    3658    3948    5049    5287    5446    5870 
   Size:        528      55     209     290    1101     238     159     424

Cuts at:   5870    6595    6946    7369    7980    8073    8147    8337    8399 
   Size:        725     351     423     611      93      74     190      62

Cuts at:   8399    9197     230 
   Size:        798    1388

  Fragments arranged by size: 

                1388    1101     798     725     611     580     528     424
                 423     353     351     321     318     302     296     290
                 261     238     209     205     190     159      93      74
                  62      55
 
BspHI T'CATG_A

Cuts at:    680    4350    8787     680 
   Size:       3670    4437    2248

  Fragments arranged by size: 

                4437    3670    2248
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1023    5122    5197    5883    6856    7405    7619    7921    8302 
   Size:       4099      75     686     973     549     214     302     381

Cuts at:   8302    1023 
   Size:       3076

  Fragments arranged by size: 

                4099    3076     973     686     549     381     302     214
                  75
 
BsrBI CCG'CTC

Cuts at:     50    1987    8647    8701   10185   10350      50 
   Size:       1937    6660      54    1484     165      55

  Fragments arranged by size: 

                6660    1937    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    657    3005    4452    4716    4907    5124    5462    5900    8267 
   Size:       2348    1447     264     191     217     338     438    2367

Cuts at:   8267     657 
   Size:       2745

  Fragments arranged by size: 

                2745    2367    2348    1447     438     338     264     217
                 191
 
BsrFI r'CCGG_y

Cuts at:   8353    8534    8353 
   Size:        181   10174
 
BsrGI T'GTAC_A

Cuts at:   1324    3847    1324 
   Size:       2523    7832
 
BssSI C'ACGA_G

Cuts at:   3665    8626    9334    3665 
   Size:       4961     708    4686

  Fragments arranged by size: 

                4961    4686     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1268    1285    2076    2824    5123    5559    6315    6672    6786 
   Size:         17     791     748    2299     436     756     357     114

Cuts at:   6786    6798    6807    6957    7732    1268 
   Size:         12       9     150     775    3891

  Fragments arranged by size: 

                3891    2299     791     775     756     748     436     357
                 150     114      17      12       9
 
BstBI TT'CG_AA

Cuts at:   1845    8716    1845 
   Size:       6871    3484
 
BstEII G'GTnAC_C

Cuts at:   5820    5820 
   Size:      10355
 
BstXI CCAn_nnnn'nTGG

Cuts at:   4644    6757    7182    7284    4644 
   Size:       2113     425     102    7715

  Fragments arranged by size: 

                7715    2113     425     102
 
BstYI r'GATC_y

Cuts at:      1    1839    1893    2024    3312    3399    3728    3825    3875 
   Size:       1838      54     131    1288      87     329      97      50

Cuts at:   3875    7001    7380    7847    7870    8205    8451    8757    8769 
   Size:       3126     379     467      23     335     246     306      12

Cuts at:   8769    8855    9907       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                3126    1838    1288    1052     467     449     379     335
                 329     306     246     131      97      87      86      54
                  50      23      12
 
BstZ17I GTA'TAC

Cuts at:   3522    4287    3522 
   Size:        765    9590
 
BtgI C'CryG_G

Cuts at:    107     593    2190    2313    3395    4103    4868    5990    5999 
   Size:        486    1597     123    1082     708     765    1122       9

Cuts at:   5999    6578    7054    7181    7263    7277     107 
   Size:        579     476     127      82      14    3185

  Fragments arranged by size: 

                3185    1597    1122    1082     765     708     579     486
                 476     127     123      82      14       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    206    8305    8486    8649     206 
   Size:       8099     181     163    1912

  Fragments arranged by size: 

                8099    1912     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1201    1248    2202    5368    5490    5888    7598    1201 
   Size:         47     954    3166     122     398    1710    3958

  Fragments arranged by size: 

                3958    3166    1710     954     398     122      47
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   4118    4153    5747    5782    4118 
   Size:         35    1594      35    8691

  Fragments arranged by size: 

                8691    1594      35      35
 
DraI TTT'AAA

Cuts at:   1907    3935    4569    7354    1907 
   Size:       2028     634    2785    4908

  Fragments arranged by size: 

                4908    2785    2028     634
 
DraIII CAC_nnn'GTG

Cuts at:   1285    4429    5063    5136    1285 
   Size:       3144     634      73    6504

  Fragments arranged by size: 

                6504    3144     634      73
 
DrdI GACnn_nn'nnGTC

Cuts at:   1337    5104    6282    8061    9405    1337 
   Size:       3767    1178    1779    1344    2287

  Fragments arranged by size: 

                3767    2287    1779    1344    1178
 
EaeI y'GGCC_r

Cuts at:    110     419    2253    5603    5685    6722    7057    7940    8114 
   Size:        309    1834    3350      82    1037     335     883     174

Cuts at:   8114    8505    8532    9877     110 
   Size:        391      27    1345     588

  Fragments arranged by size: 

                3350    1834    1345    1037     883     588     391     335
                 309     174      82      27
 
EagI C'GGCC_G

Cuts at:   7940    7940 
   Size:      10355
 
EarI CTCTTCn'nnn_

Cuts at:    779    2309    2591    3140    3382    4676    5037    8378    8588 
   Size:       1530     282     549     242    1294     361    3341     210

Cuts at:   8588     779 
   Size:       2546

  Fragments arranged by size: 

                3341    2546    1530    1294     549     361     282     242
                 210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    174    4891    8563    9305    9451   10165     174 
   Size:       4717    3672     742     146     714     364

  Fragments arranged by size: 

                4717    3672     742     714     364     146
 
EcoICRI GAG'CTC

Cuts at:   2338    7367    2338 
   Size:       5029    5326
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    125     283     760     907    1043    1103    1421    2290    2354 
   Size:        158     477     147     136      60     318     869      64

Cuts at:   2354    2459    2519    3188    3212    4601    5044    6111    6553 
   Size:        105      60     669      24    1389     443    1067     442

Cuts at:   6553    6886    7065    8180    8612    8965    9627     125 
   Size:        333     179    1115     432     353     662     853

  Fragments arranged by size: 

                1389    1115    1067     869     853     669     662     477
                 443     442     432     353     333     318     179     158
                 147     136     105      64      60      60      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:    449     995    4058     449 
   Size:        546    3063    6746

  Fragments arranged by size: 

                6746    3063     546
 
EcoO109I rG'GnC_Cy

Cuts at:    544     820    1768    2122    5643    5866    5867    6031    6712 
   Size:        276     948     354    3521     223       1     164     681

Cuts at:   6712    6917    7494     544 
   Size:        205     577    3405

  Fragments arranged by size: 

                3521    3405     948     681     577     354     276     223
                 205     164       1
 
EcoRI G'AATT_C

Cuts at:   1805    2595    2994    7215    7314    7359    1805 
   Size:        790     399    4221      99      45    4801

  Fragments arranged by size: 

                4801    4221     790     399      99      45
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   8374    8374 
   Size:      10355
 
FspI TGC'GCA

Cuts at:   8136    8136 
   Size:      10355
 
HaeII r_GCGC'y

Cuts at:   1167    5319    5928    7277    7789    8037    9267   10038    1167 
   Size:       4152     609    1349     512     248    1230     771    1484

  Fragments arranged by size: 

                4152    1484    1349    1230     771     609     512     248
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    193     225     558     590     756     788    1477    1509    1626 
   Size:         32     333      32     166      32     689      32     117

Cuts at:   1626    1658    2202    2234    3304    3336    4968    5000    6015 
   Size:         32     544      32    1070      32    1632      32    1015

Cuts at:   6015    6047    6777    6809    7089    7121    8197    8227    8229 
   Size:         32     730      32     280      32    1076      30       2

Cuts at:   8229    8259     193 
   Size:         30    2289

  Fragments arranged by size: 

                2289    1632    1076    1070    1015     730     689     544
                 333     280     166     117      32      32      32      32
                  32      32      32      32      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:    557    1018    1447    3337    4215    6175    7394     557 
   Size:        461     429    1890     878    1960    1219    3518

  Fragments arranged by size: 

                3518    1960    1890    1219     878     461     429
 
KasI G'GCGC_C

Cuts at:   7785    8033    7785 
   Size:        248   10107
 
KpnI G_GTAC'C

Cuts at:   7375    7375 
   Size:      10355
 
MfeI C'AATT_G

Cuts at:    692    5361     692 
   Size:       4669    5686
 
MluI A'CGCG_T

Cuts at:   9788    9788 
   Size:      10355
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1392    2408    3590    5332    5695    5854    5988    9114    9298 
   Size:       1016    1182    1742     363     159     134    3126     184

Cuts at:   9298    1392 
   Size:       2449

  Fragments arranged by size: 

                3126    2449    1742    1182    1016     363     184     159
                 134
 
MscI TGG'CCA

Cuts at:    421    2255    5605    5687    6724    8116     421 
   Size:       1834    3350      82    1037    1392    2660

  Fragments arranged by size: 

                3350    2660    1834    1392    1037      82
 
MslI CAynn'nnrTG

Cuts at:    517     598     610    2989    3707    4723    5065    5071    6004 
   Size:         81      12    2379     718    1016     342       6     933

Cuts at:   6004    8344    8471     517 
   Size:       2340     127    2401

  Fragments arranged by size: 

                2401    2379    2340    1016     933     718     342     127
                  81      12       6
 
MspA1I CmG'CkG

Cuts at:    348    1096    2465    2898    2938    5559    5616    5914    6345 
   Size:        748    1369     433      40    2621      57     298     431

Cuts at:   6345    6369    6783    7466    7780    8140    8924    9169     348 
   Size:         24     414     683     314     360     784     245    1534

  Fragments arranged by size: 

                2621    1534    1369     784     748     683     433     431
                 414     360     314     298     245      57      40      24
            
 
NaeI GCC'GGC

Cuts at:   8536    8536 
   Size:      10355
 
NarI GG'CG_CC

Cuts at:   7786    8034    7786 
   Size:        248   10107
 
NcoI C'CATG_G

Cuts at:    107     593    2313    3395    4868    5990    5999    6578    7181 
   Size:        486    1720    1082    1473    1122       9     579     603

Cuts at:   7181    7263     107 
   Size:         82    3199

  Fragments arranged by size: 

                3199    1720    1473    1122    1082     603     579     486
                  82       9
 
NdeI CA'TA_TG

Cuts at:   1938    6956    1938 
   Size:       5018    5337
 
NgoMIV G'CCGG_C

Cuts at:   8534    8534 
   Size:      10355
 
NheI G'CTAG_C

Cuts at:   7237    7237 
   Size:      10355
 
NsiI A_TGCA'T

Cuts at:   3872    4238    3872 
   Size:        366    9989
 
NspI r_CATG'y

Cuts at:   1150    1617    1965    2079    4236    4240    4421    5070    5613 
   Size:        467     348     114    2157       4     181     649     543

Cuts at:   5613    5757    6426    6801    7408    7908    9511    1150 
   Size:        144     669     375     607     500    1603    1994

  Fragments arranged by size: 

                2157    1994    1603     669     649     607     543     500
                 467     375     348     181     144     114       4
 
PasI CC'CwG_GG

Cuts at:   1764    5799    6409    6429    1764 
   Size:       4035     610      20    5690

  Fragments arranged by size: 

                5690    4035     610      20
 
PciI A'CATG_T

Cuts at:   1961    9507    1961 
   Size:       7546    2809
 
PflMI CCAn_nnn'nTGG

Cuts at:   1074    2236    5220    5331    6348    6852    7153    1074 
   Size:       1162    2984     111    1017     504     301    4276

  Fragments arranged by size: 

                4276    2984    1162    1017     504     301     111
 
PfoI T'CCnGG_A

Cuts at:    444     630     888    3228    7152     444 
   Size:        186     258    2340    3924    3647

  Fragments arranged by size: 

                3924    3647    2340     258     186
 
PmeI GTTT'AAAC

Cuts at:   7354    7354 
   Size:      10355
 
PmlI CAC'GTG

Cuts at:   5940    5940 
   Size:      10355
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   8801    8833    8801 
   Size:         32   10323
 
PpuMI rG'GwC_Cy

Cuts at:    544    1768    6031    6917     544 
   Size:       1224    4263     886    3982

  Fragments arranged by size: 

                4263    3982    1224     886
 
PshAI GACnn'nnGTC

Cuts at:   4342    4342 
   Size:      10355
 
PspOMI G'GGCC_C

Cuts at:   5866    5866 
   Size:      10355
 
PstI C_TGCA'G

Cuts at:    648    2094    4344    4499    5274    5564    5880    6305    7402 
   Size:       1446    2250     155     775     290     316     425    1097

Cuts at:   7402    8087     648 
   Size:        685    2916

  Fragments arranged by size: 

                2916    2250    1446    1097     775     685     425     316
                 290     155
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:      10355
 
PvuII CAG'CTG

Cuts at:    348    1096    2938    5559    6345    7780    8140     348 
   Size:        748    1842    2621     786    1435     360    2563

  Fragments arranged by size: 

                2621    2563    1842    1435     786     748     360
 
RsrII CG'GwC_CG

Cuts at:   8550    8550 
   Size:      10355
 
SacI G_AGCT'C

Cuts at:   2340    7369    2340 
   Size:       5029    5326
 
SalI G'TCGA_C

Cuts at:   7392    7392 
   Size:      10355
 
SanDI GG'GwC_CC

Cuts at:   6917    6917 
   Size:      10355
 
SapI GCTCTTCn'nnn_

Cuts at:   2591    3382    5037    8378    8588    2591 
   Size:        791    1655    3341     210    4358

  Fragments arranged by size: 

                4358    3341    1655     791     210
 
SbfI CC_TGCA'GG

Cuts at:   7402    7402 
   Size:      10355
 
ScaI AGT'ACT

Cuts at:   3201    9872    3201 
   Size:       6671    3684
 
SfcI C'TryA_G

Cuts at:     33     257     644    2062    2090    4340    4495    5270    5560 
   Size:        224     387    1418      28    2250     155     775     290

Cuts at:   5560    5572    5876    6124    6301    6445    6877    7398    8083 
   Size:         12     304     248     177     144     432     521     685

Cuts at:   8083    9051    9242      33 
   Size:        968     191    1146

  Fragments arranged by size: 

                2250    1418    1146     968     775     685     521     432
                 387     304     290     248     224     191     177     155
                 144      28      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    427     427 
   Size:      10355
 
SfoI GGC'GCC

Cuts at:   7787    8035    7787 
   Size:        248   10107
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10355
 
SmaI CCC'GGG

Cuts at:   4821    7377    4821 
   Size:       2556    7799
 
SmlI C'TyrA_G

Cuts at:    832    2483    4332    5777    6654    7510    8425    9139    9401 
   Size:       1651    1849    1445     877     856     915     714     262

Cuts at:   9401     832 
   Size:       1786

  Fragments arranged by size: 

                1849    1786    1651    1445     915     877     856     714
                 262
 
SpeI A'CTAG_T

Cuts at:     67     526    2977      67 
   Size:        459    2451    7445

  Fragments arranged by size: 

                7445    2451     459
 
SphI G_CATG'C

Cuts at:   1150    1617    4236    5070    5613    5757    6426    7408    7908 
   Size:        467    2619     834     543     144     669     982     500

Cuts at:   7908    1150 
   Size:       3597

  Fragments arranged by size: 

                3597    2619     982     834     669     543     500     467
                 144
 
StuI AGG'CCT

Cuts at:   1049    5234    6850    6883    1049 
   Size:       4185    1616      33    4521

  Fragments arranged by size: 

                4521    4185    1616      33
 
StyI C'CwwG_G

Cuts at:    107     593     915    1068    1608    2313    2752    3395    3585 
   Size:        486     322     153     540     705     439     643     190

Cuts at:   3585    3732    4730    4868    5235    5411    5990    5999    6096 
   Size:        147     998     138     367     176     579       9      97

Cuts at:   6096    6139    6578    6750    6851    7093    7181    7263    7489 
   Size:         43     439     172     101     242      88      82     226

Cuts at:   7489    7843     107 
   Size:        354    2619

  Fragments arranged by size: 

                2619     998     705     643     579     540     486     439
                 439     367     354     322     242     226     190     176
                 172     153     147     138     101      97      88      82
                  43       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   4284    5293    6078    6591    7938    8071    8530    8739    4284 
   Size:       1009     785     513    1347     133     459     209    5900

  Fragments arranged by size: 

                5900    1347    1009     785     513     459     209     133
            
 
TatI w'GTAC_w

Cuts at:    942    1324    3199    3847    5155    7333    9870     942 
   Size:        382    1875     648    1308    2178    2537    1427

  Fragments arranged by size: 

                2537    2178    1875    1427    1308     648     382
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3810    3842    6389    6421    7111    7143    9872    9904    3810 
   Size:         32    2547      32     690      32    2729      32    4261

  Fragments arranged by size: 

                4261    2729    2547     690      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   8152    8152 
   Size:      10355
 
XbaI T'CTAG_A

Cuts at:   4011    7386    4011 
   Size:       3375    6980
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    516    3054    5067    5997    6042    6156    7850     516 
   Size:       2538    2013     930      45     114    1694    3021

  Fragments arranged by size: 

                3021    2538    2013    1694     930     114      45
 
XmaI C'CCGG_G

Cuts at:   4819    7375    4819 
   Size:       2556    7799
 
XmnI GAAnn'nnTTC

Cuts at:   2154    3323    3718    4163    2154 
   Size:       1169     395     445    8346

  Fragments arranged by size: 

                8346    1169     445     395
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AgeI     AloI     AscI    AvrII    BmgBI    BsiWI    BspEI
   BssHII   Bsu36I     ClaI    EcoRV     FseI    FspAI  HindIII     HpaI
     NotI     NruI     PacI     PsiI    PspXI     PsrI    SacII    SexAI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     ZraI
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