Restriction map for pF1KB4802
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AhdI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvrII       BbrPI       BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsiEI       BsiWI       Bsp1407I    BspDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HpaI        
KpnI        KspI        MamI        MluI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TthHB8I     
XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         1799  GTAGAC               GT^[AC][GT]AC
AcsI          620  AAATTC               [GA]^AATT[CT]
AflIII        970  ACATGT               A^C[GA][CT]GT
AgeI         3269  ACCGGT               A^CCGGT
Alw44I        878  GTGCAC               G^TGCAC
ApaLI         878  GTGCAC               G^TGCAC
ApoI          620  AAATTC               [GA]^AATT[CT]
AspI         2022  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         775  TGCGCA               TGC^GCA
BbeI         1097  GGCGCC               GGCGC^C
BfaI         1873  CTAG                 C^TAG
BsmI          934  GAATGC               GAATGC(1/-1)
BspHI         356  TCATGA               T^CATGA
BspLU11I      970  ACATGT               A^CATGT
EarI         2094  GAAGAG               CTCTTC(1/4)
EcoT14I      3356  CCATGG               C^C[AT][AT]GG
FspI          775  TGCGCA               TGC^GCA
KasI         1097  GGCGCC               G^GCGCC
Ksp632I      2094  GAAGAG               CTCTTC(1/4)
MaeI         1873  CTAG                 C^TAG
MfeI         1032  CAATTG               C^AATTG
MseI          572  TTAA                 T^TAA
MunI         1032  CAATTG               C^AATTG
NarI         1097  GGCGCC               GG^CGCC
NcoI         3356  CCATGG               C^CATGG
NdeI         1013  CATATG               CA^TATG
PflFI        2022  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3269  ACCGGT               A^CCGGT
RcaI          356  TCATGA               T^CATGA
SacI         3133  GAGCTC               GAGCT^C
SfoI         1097  GGCGCC               GGC^GCC
SmlI          648  CTTGAG               C^T[CT][GA]AG
StuI          535  AGGCCT               AGG^CCT
StyI         3356  CCATGG               C^C[AT][AT]GG
Tru9I         572  TTAA                 T^TAA
Tth111I      2022  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcyI          925  GACGCC               G[GA]^CG[CT]C
             1097  GGCGCC              
AocI         1971  CCTCAGG              CC^T[ACGT]AGG
             2307  CCTGAGG             
BamHI        1868  GGATCC               G^GATCC
             3311  GGATCC              
BbsI         3324  GAAGAC               GAAGAC(2/6)
             2322  GTCTTC              
BpuAI        3324  GAAGAC               GAAGAC(2/6)
             2322  GTCTTC              
BsaHI         925  GACGCC               G[GA]^CG[CT]C
             1097  GGCGCC              
BsmBI        1574  GAGACG               CGTCTC(1/5)
             2020  GAGACG              
Bsu36I       1971  CCTCAGG              CC^T[ACGT]AGG
             2307  CCTGAGG             
Eco81I       1971  CCTCAGG              CC^T[ACGT]AGG
             2307  CCTGAGG             
EcoNI        2010  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2958  CCTGCGGCAGG         
HgaI          925  GACGC                GACGC(5/10)
             2657  GACGC               
Hin1I         925  GACGCC               G[GA]^CG[CT]C
             1097  GGCGCC              
HindIII      2061  AAGCTT               A^AGCTT
             2296  AAGCTT              
NaeI          385  GCCGGC               GCC^GGC
              856  GCCGGC              
NgoMIV        385  GCCGGC               G^CCGGC
              856  GCCGGC              
SfiI         2472  GGCCGGTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             2742  GGCCGTGCTGGCC       
SmaI         1101  CCCGGG               CCC^GGG
             1629  CCCGGG              
SphI           45  GCATGC               GCATG^C
              389  GCATGC              
XmaCI        1101  CCCGGG               C^CCGGG
             1629  CCCGGG              
XmaI         1101  CCCGGG               C^CCGGG
             1629  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AccIII      AciI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvCI       BbvI        BcgI        BcnI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eco47III    EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MroI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI        XhoII       
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