Restriction map for pF1KB4802
 (Linear) (Six-base) MAPSORT of: KIBB4802.seq  Check: 8327  from: 1  to: 3516



KIBB4802.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2490    3516 
   Size:       2490    1026
 
AccI GT'mk_AC

Cuts at:      0    1800    3516 
   Size:       1800    1716
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2117    3516 
   Size:       2117    1399
 
AfeI AGC'GCT

Cuts at:      0     403     874    1225    1536    2605    3516 
   Size:        403     471     351     311    1069     911

  Fragments arranged by size: 

                1069     911     471     403     351     311
 
AflIII A'CryG_T

Cuts at:      0     970    3516 
   Size:        970    2546
 
AgeI A'CCGG_T

Cuts at:      0    3269    3516 
   Size:       3269     247
 
AleI CACnn'nnGTG

Cuts at:      0     814    1289    3516 
   Size:        814     475    2227

  Fragments arranged by size: 

                2227     814     475
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     869    2882    3516 
   Size:        869    2013     634

  Fragments arranged by size: 

                2013     869     634
 
AlwNI CAG_nnn'CTG

Cuts at:      0     444     525    1788    1837    1992    2779    3063    3516 
   Size:        444      81    1263      49     155     787     284     453

  Fragments arranged by size: 

                1263     787     453     444     284     155      81      49
            
 
ApaI G_GGCC'C

Cuts at:      0     377    1630    1685    3516 
   Size:        377    1253      55    1831

  Fragments arranged by size: 

                1831    1253     377      55
 
ApaLI G'TGCA_C

Cuts at:      0     878    3516 
   Size:        878    2638
 
ApoI r'AATT_y

Cuts at:      0     620    3516 
   Size:        620    2896
 
AvaI C'yCGr_G

Cuts at:      0     473    1101    1629    3516 
   Size:        473     628     528    1887

  Fragments arranged by size: 

                1887     628     528     473
 
BamHI G'GATC_C

Cuts at:      0    1868    3311    3516 
   Size:       1868    1443     205

  Fragments arranged by size: 

                1868    1443     205
 
BanI G'GyrC_C

Cuts at:      0     515    1097    2281    3007    3196    3304    3516 
   Size:        515     582    1184     726     189     108     212

  Fragments arranged by size: 

                1184     726     582     515     212     189     108
 
BanII G_rGCy'C

Cuts at:      0     377    1170    1630    1685    2919    3137    3516 
   Size:        377     793     460      55    1234     218     379

  Fragments arranged by size: 

                1234     793     460     379     377     218      55
 
BbeI G_GCGC'C

Cuts at:      0    1101    3516 
   Size:       1101    2415
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2315    3331    3516 
   Size:       2315    1016     185

  Fragments arranged by size: 

                2315    1016     185
 
BbvCI CC'TCA_GC

Cuts at:      0    1465    2891    2897    3047    3516 
   Size:       1465    1426       6     150     469

  Fragments arranged by size: 

                1465    1426     469     150       6
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     493     527    2557    2591    2695    2729    3516 
   Size:        493      34    2030      34     104      34     787

  Fragments arranged by size: 

                2030     787     493     104      34      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     534    1670    1706    2479    2749    3516 
   Size:        534    1136      36     773     270     767

  Fragments arranged by size: 

                1136     773     767     534     270      36
 
BlpI GC'TnA_GC

Cuts at:      0    2152    2186    2507    3429    3516 
   Size:       2152      34     321     922      87

  Fragments arranged by size: 

                2152     922     321      87      34
 
Bme1580I G_kGCm'C

Cuts at:      0     377     683     882     962    1501    1630    1685    2601 
   Size:        377     306     199      80     539     129      55     916

Cuts at:   2601    2877    3222    3309    3516 
   Size:        276     345      87     207

  Fragments arranged by size: 

                 916     539     377     345     306     276     207     199
                 129      87      80      55
 
BmgBI CAC'GTC

Cuts at:      0    1578    3516 
   Size:       1578    1938
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     165    1174    3516 
   Size:        165    1009    2342

  Fragments arranged by size: 

                2342    1009     165
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     423    1927    3516 
   Size:        423    1504    1589

  Fragments arranged by size: 

                1589    1504     423
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     924    1864    1969    2032    2065    2335    2355 
   Size:        195     729     940     105      63      33     270      20

Cuts at:   2355    2521    3469    3516 
   Size:        166     948      47

  Fragments arranged by size: 

                 948     940     729     270     195     166     105      63
                  47      33      20
 
Bpu10I CC'TnA_GC

Cuts at:      0    1465    1946    2224    2891    2897    2990    3047    3516 
   Size:       1465     481     278     667       6      93      57     469

  Fragments arranged by size: 

                1465     667     481     469     278      93      57       6
            
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     669    3516 
   Size:        669    2847
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     900    1114    2033    2077    2199    2605    3346 
   Size:        151     749     214     919      44     122     406     741

Cuts at:   3346    3516 
   Size:        170

  Fragments arranged by size: 

                 919     749     741     406     214     170     151     122
                  44
 
BsaHI Gr'CG_yC

Cuts at:      0     926    1098    3516 
   Size:        926     172    2418

  Fragments arranged by size: 

                2418     926     172
 
BsaWI w'CCGG_w

Cuts at:      0    1088    1146    1562    2144    3269    3516 
   Size:       1088      58     416     582    1125     247

  Fragments arranged by size: 

                1125    1088     582     416     247      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475    2008    2011    2038    2041    3516 
   Size:        445      30    1533       3      27       3    1475

  Fragments arranged by size: 

                1533    1475     445      30      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     396    1351    2029    2032    2285    2288    2291    3145 
   Size:        396     955     678       3     253       3       3     854

Cuts at:   3145    3352    3516 
   Size:        207     164

  Fragments arranged by size: 

                 955     854     678     396     253     207     164       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     703     725    2531    2600    3105    3179    3291    3516 
   Size:        703      22    1806      69     505      74     112     225

  Fragments arranged by size: 

                1806     703     505     225     112      74      69      22
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     441     838    1063    1234    1551    3516 
   Size:        150     291     397     225     171     317    1965

  Fragments arranged by size: 

                1965     397     317     291     225     171     150
 
BsiHKAI G_wGCw'C

Cuts at:      0     882    1398    2158    3137    3497    3516 
   Size:        882     516     760     979     360      19

  Fragments arranged by size: 

                 979     882     760     516     360      19
 
BsmI GAATG_Cn'

Cuts at:      0     940    3516 
   Size:        940    2576
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1568    2014    3516 
   Size:       1568     446    1502

  Fragments arranged by size: 

                1568    1502     446
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     683     882     962    1170    1398    1501    1630 
   Size:        377     306     199      80     208     228     103     129

Cuts at:   1630    1685    2158    2601    2877    2919    3137    3222    3309 
   Size:         55     473     443     276      42     218      85      87

Cuts at:   3309    3497    3516 
   Size:        188      19

  Fragments arranged by size: 

                 473     443     377     306     276     228     218     208
                 199     188     129     103      87      85      80      55
                  42      19
 
BspEI T'CCGG_A

Cuts at:      0    1088    1146    2144    3516 
   Size:       1088      58     998    1372

  Fragments arranged by size: 

                1372    1088     998      58
 
BspHI T'CATG_A

Cuts at:      0     356    3516 
   Size:        356    3160
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     810    1132    2181    2490    2670    2966    3048 
   Size:        104     706     322    1049     309     180     296      82

Cuts at:   3048    3516 
   Size:        468

  Fragments arranged by size: 

                1049     706     468     322     309     296     180     104
                  82
 
BsrBI CCG'CTC

Cuts at:      0    1068    2633    3017    3516 
   Size:       1068    1565     384     499

  Fragments arranged by size: 

                1565    1068     499     384
 
BsrDI GCAATG_nn'

Cuts at:      0     960    1815    2810    2864    3516 
   Size:        960     855     995      54     652

  Fragments arranged by size: 

                 995     960     855     652      54
 
BsrFI r'CCGG_y

Cuts at:      0     385     856     896     909    1094    2284    2473    2879 
   Size:        385     471      40      13     185    1190     189     406

Cuts at:   2879    3269    3516 
   Size:        390     247

  Fragments arranged by size: 

                1190     471     406     390     385     247     189     185
                  40      13
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1133    1788    3063    3217    3516 
   Size:       1133     655    1275     154     299

  Fragments arranged by size: 

                1275    1133     655     299     154
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1371    2301    2379    2428    3105    3484    3516 
   Size:       1371     930      78      49     677     379      32

  Fragments arranged by size: 

                1371     930     677     379      78      49      32
 
BstYI r'GATC_y

Cuts at:      0    1868    1898    2001    2801    3311    3467    3516 
   Size:       1868      30     103     800     510     156      49

  Fragments arranged by size: 

                1868     800     510     156     103      49      30
 
Bsu36I CC'TnA_GG

Cuts at:      0    1972    2308    3516 
   Size:       1972     336    1208

  Fragments arranged by size: 

                1972    1208     336
 
BtgI C'CryG_G

Cuts at:      0      66    1851    3356    3516 
   Size:         66    1785    1505     160

  Fragments arranged by size: 

                1785    1505     160      66
 
BtsI GCAGTG_nn'

Cuts at:      0     851    1163    1198    1501    2127    2930    2977    3516 
   Size:        851     312      35     303     626     803      47     539

  Fragments arranged by size: 

                 851     803     626     539     312     303      47      35
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2343    2378    3516 
   Size:       2343      35    1138

  Fragments arranged by size: 

                2343    1138      35
 
EaeI y'GGCC_r

Cuts at:      0     759    1415    1422    1580    1952    3067    3516 
   Size:        759     656       7     158     372    1115     449

  Fragments arranged by size: 

                1115     759     656     449     372     158       7
 
EarI CTCTTCn'nnn_

Cuts at:      0    2089    3516 
   Size:       2089    1427
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     469     928    1537    3127    3516 
   Size:        469     459     609    1590     389

  Fragments arranged by size: 

                1590     609     469     459     389
 
EcoICRI GAG'CTC

Cuts at:      0    3135    3516 
   Size:       3135     381
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     924    1864    1969    2032    2065    2117    2335 
   Size:        195     729     940     105      63      33      52     218

Cuts at:   2335    2355    2521    3469    3516 
   Size:         20     166     948      47

  Fragments arranged by size: 

                 948     940     729     218     195     166     105      63
                  52      47      33      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2014    2962    3516 
   Size:       2014     948     554

  Fragments arranged by size: 

                2014     948     554
 
EcoO109I rG'GnC_Cy

Cuts at:      0      71      93     110     373     791     802    1310    1375 
   Size:         71      22      17     263     418      11     508      65

Cuts at:   1375    1626    1682    1785    1834    1989    2480    3516 
   Size:        251      56     103      49     155     491    1036

  Fragments arranged by size: 

                1036     508     491     418     263     251     155     103
                  71      65      56      49      22      17      11
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1263    3516 
   Size:       1263    2253
 
FspI TGC'GCA

Cuts at:      0     777    3516 
   Size:        777    2739
 
HaeII r_GCGC'y

Cuts at:      0     405     710     876    1101    1227    1350    1538    2607 
   Size:        405     305     166     225     126     123     188    1069

Cuts at:   2607    2627    3516 
   Size:         20     889

  Fragments arranged by size: 

                1069     889     405     305     225     188     166     126
                 123      20
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     574     606    1630    1662    2011    2043 
   Size:        445      32      97      32    1024      32     349      32

Cuts at:   2043    3516 
   Size:       1473

  Fragments arranged by size: 

                1473    1024     445     349      97      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0    2061    2296    3516 
   Size:       2061     235    1220

  Fragments arranged by size: 

                2061    1220     235
 
KasI G'GCGC_C

Cuts at:      0    1097    3516 
   Size:       1097    2419
 
MfeI C'AATT_G

Cuts at:      0    1032    3516 
   Size:       1032    2484
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     591    2222    3371    3516 
   Size:        591    1631    1149     145

  Fragments arranged by size: 

                1631    1149     591     145
 
MscI TGG'CCA

Cuts at:      0    1417    1424    1582    1954    3516 
   Size:       1417       7     158     372    1562

  Fragments arranged by size: 

                1562    1417     372     158       7
 
MslI CAynn'nnrTG

Cuts at:      0     761     814    1289    3361    3482    3516 
   Size:        761      53     475    2072     121      34

  Fragments arranged by size: 

                2072     761     475     121      53      34
 
MspA1I CmG'CkG

Cuts at:      0     321     814     988    1077    1289    1655    1688    1998 
   Size:        321     493     174      89     212     366      33     310

Cuts at:   1998    2450    2514    2541    3105    3433    3516 
   Size:        452      64      27     564     328      83

  Fragments arranged by size: 

                 564     493     452     366     328     321     310     212
                 174      89      83      64      33      27
 
NaeI GCC'GGC

Cuts at:      0     387     858    3516 
   Size:        387     471    2658

  Fragments arranged by size: 

                2658     471     387
 
NarI GG'CG_CC

Cuts at:      0    1098    3516 
   Size:       1098    2418
 
NcoI C'CATG_G

Cuts at:      0    3356    3516 
   Size:       3356     160
 
NdeI CA'TA_TG

Cuts at:      0    1014    3516 
   Size:       1014    2502
 
NgoMIV G'CCGG_C

Cuts at:      0     385     856    3516 
   Size:        385     471    2660

  Fragments arranged by size: 

                2660     471     385
 
NspI r_CATG'y

Cuts at:      0      49     393     974    1213    1326    3516 
   Size:         49     344     581     239     113    2190

  Fragments arranged by size: 

                2190     581     344     239     113      49
 
PasI CC'CwG_GG

Cuts at:      0    2468    3516 
   Size:       2468    1048
 
PciI A'CATG_T

Cuts at:      0     970    3516 
   Size:        970    2546
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      88     278     444    1826    2607    2925    3516 
   Size:         88     190     166    1382     781     318     591

  Fragments arranged by size: 

                1382     781     591     318     190     166      88
 
PfoI T'CCnGG_A

Cuts at:      0    1312    1775    1864    2684    2726    3032    3516 
   Size:       1312     463      89     820      42     306     484

  Fragments arranged by size: 

                1312     820     484     463     306      89      42
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     295     327    3516 
   Size:        295      32    3189

  Fragments arranged by size: 

                3189     295      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      93     110    1310    1375    3516 
   Size:         93      17    1200      65    2141

  Fragments arranged by size: 

                2141    1200      93      65      17
 
PspOMI G'GGCC_C

Cuts at:      0     373    1626    1681    3516 
   Size:        373    1253      55    1835

  Fragments arranged by size: 

                1835    1253     373      55
 
PstI C_TGCA'G

Cuts at:      0     320     740     801    1542    1997    2455    2789    2984 
   Size:        320     420      61     741     455     458     334     195

Cuts at:   2984    3516 
   Size:        532

  Fragments arranged by size: 

                 741     532     458     455     420     334     320     195
                  61
 
PvuII CAG'CTG

Cuts at:      0     321    1077    1998    2514    3105    3516 
   Size:        321     756     921     516     591     411

  Fragments arranged by size: 

                 921     756     591     516     411     321
 
SacI G_AGCT'C

Cuts at:      0    3137    3516 
   Size:       3137     379
 
SanDI GG'GwC_CC

Cuts at:      0    1310    1375    3516 
   Size:       1310      65    2141

  Fragments arranged by size: 

                2141    1310      65
 
SfcI C'TryA_G

Cuts at:      0     231     316     736     797    1538    1993    2451    2785 
   Size:        231      85     420      61     741     455     458     334

Cuts at:   2785    2980    3516 
   Size:        195     536

  Fragments arranged by size: 

                 741     536     458     455     420     334     231     195
                  85      61
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2479    2749    3516 
   Size:       2479     270     767

  Fragments arranged by size: 

                2479     767     270
 
SfoI GGC'GCC

Cuts at:      0    1099    3516 
   Size:       1099    2417
 
SmaI CCC'GGG

Cuts at:      0    1103    1631    3516 
   Size:       1103     528    1885

  Fragments arranged by size: 

                1885    1103     528
 
SmlI C'TyrA_G

Cuts at:      0     648    3516 
   Size:        648    2868
 
SphI G_CATG'C

Cuts at:      0      49     393    3516 
   Size:         49     344    3123

  Fragments arranged by size: 

                3123     344      49
 
SrfI GCCC'GGGC

Cuts at:      0    1631    3516 
   Size:       1631    1885
 
StuI AGG'CCT

Cuts at:      0     537    3516 
   Size:        537    2979
 
StyI C'CwwG_G

Cuts at:      0    3356    3516 
   Size:       3356     160
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184     795    2358    3097    3283    3516 
   Size:        184     611    1563     739     186     233

  Fragments arranged by size: 

                1563     739     611     233     186     184
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     499     531    1312    1344    3303    3335    3516 
   Size:        499      32     781      32    1959      32     181

  Fragments arranged by size: 

                1959     781     499     181      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2025    3516 
   Size:       2025    1491
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1012    2835    3363    3516 
   Size:       1012    1823     528     153

  Fragments arranged by size: 

                1823    1012     528     153
 
XmaI C'CCGG_G

Cuts at:      0    1101    1629    3516 
   Size:       1101     528    1887

  Fragments arranged by size: 

                1887    1101     528
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
  HindIII     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     SrfI     StuI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AhdI     AloI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI    BglII     BmtI
    BsaAI    BsaBI    BsiEI    BsiWI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
    EcoRI    EcoRV     FseI    FspAI   HincII     HpaI     KpnI     MluI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SspI
     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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