Restriction map for
pF1KB4786
(Circular) (Six-base) MAPSORT of: pF1KB4786.seq Check: 3860 from: 1 to: 7505
pF1KB4786 7505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 232 3889 232
Size: 3657 3848
AatII G_ACGT'C
Cuts at: 2862 2862
Size: 7505
AccI GT'mk_AC
Cuts at: 4543 4543
Size: 7505
Acc65I G'GTAC_C
Cuts at: 245 4521 245
Size: 4276 3229
AclI AA'CG_TT
Cuts at: 7345 7345
Size: 7505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1217 2269 3684 5330 5762 6115 6777 731
Size: 486 1052 1415 1646 432 353 662 1459
Fragments arranged by size:
1646 1459 1415 1052 662 486 432 353
AfeI AGC'GCT
Cuts at: 2709 3687 2709
Size: 978 6527
AflIII A'CryG_T
Cuts at: 260 4857 6657 6938 260
Size: 4597 1800 281 827
Fragments arranged by size:
4597 1800 827 281
AleI CACnn'nnGTG
Cuts at: 3740 3740
Size: 7505
AlwNI CAG_nnn'CTG
Cuts at: 4810 6248 4810
Size: 1438 6067
ApaI G_GGCC'C
Cuts at: 1015 1106 1187 1375 3112 3599 1015
Size: 91 81 188 1737 487 4921
Fragments arranged by size:
4921 1737 487 188 91 81
ApaLI G'TGCA_C
Cuts at: 689 6343 689
Size: 5654 1851
ApoI r'AATT_y
Cuts at: 629 4465 4509 6858 7114 629
Size: 3836 44 2349 256 1020
Fragments arranged by size:
3836 2349 1020 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 7505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7505
AvaI C'yCGr_G
Cuts at: 271 367 464 764 863 1187 1349 1520 2195
Size: 96 97 300 99 324 162 171 675
Cuts at: 2195 2953 3527 3793 4525 271
Size: 758 574 266 732 3251
Fragments arranged by size:
3251 758 732 675 574 324 300 266
171 162 99 97 96
BamHI G'GATC_C
Cuts at: 2911 4530 2911
Size: 1619 5886
BanI G'GyrC_C
Cuts at: 245 355 931 1300 1345 1714 1822 2110 2245
Size: 110 576 369 45 369 108 288 135
Cuts at: 2245 2272 2434 2497 3163 3199 3262 3352 4450
Size: 27 162 63 666 36 63 90 1098
Cuts at: 4450 4521 4935 5183 5218 245
Size: 71 414 248 35 2532
Fragments arranged by size:
2532 1098 666 576 414 369 369 288
248 162 135 110 108 90 71 63
63 45 36 35 27
BanII G_rGCy'C
Cuts at: 278 999 1015 1053 1106 1187 1375 1521 1883
Size: 721 16 38 53 81 188 146 362
Cuts at: 1883 1962 2259 2493 3112 3159 3599 3850 4135
Size: 79 297 234 619 47 440 251 285
Cuts at: 4135 4285 4519 5549 278
Size: 150 234 1030 2234
Fragments arranged by size:
2234 1030 721 619 440 362 297 285
251 234 234 188 150 146 81 79
53 47 38 16
BbeI G_GCGC'C
Cuts at: 359 2114 2276 3203 3356 4939 5187 359
Size: 1755 162 927 153 1583 248 2677
Fragments arranged by size:
2677 1755 1583 927 248 162 153
BbsI GAAGACnn'nnnn_
Cuts at: 212 2311 3022 3966 212
Size: 2099 711 944 3751
Fragments arranged by size:
3751 2099 944 711
BciVI GTATCCnnnnn_n'
Cuts at: 5403 6459 7505 5403
Size: 1056 1046 5403
Fragments arranged by size:
5403 1056 1046
BclI T'GATC_A
Cuts at: 5025 5025
Size: 7505
BglI GCCn_nnn'nGGC
Cuts at: 398 467 494 503 849 1019 1235 1379 1658
Size: 69 27 9 346 170 216 144 279
Cuts at: 1658 2018 2540 2900 3278 3287 3350 3476 3854
Size: 360 522 360 378 9 63 126 378
Cuts at: 3854 4150 398
Size: 296 3753
Fragments arranged by size:
3753 522 378 378 360 360 346 296
279 216 170 144 126 69 63 27
9 9
BglII A'GATC_T
Cuts at: 5020 7057 5020
Size: 2037 5468
BlpI GC'TnA_GC
Cuts at: 4617 4617
Size: 7505
Bme1580I G_kGCm'C
Cuts at: 693 1015 1106 1187 1350 1375 1827 2250 2439
Size: 322 91 81 163 25 452 423 189
Cuts at: 2439 2502 3112 3267 3599 4455 5130 5223 6347
Size: 63 610 155 332 856 675 93 1124
Cuts at: 6347 693
Size: 1851
Fragments arranged by size:
1851 1124 856 675 610 452 423 332
322 189 163 155 93 91 81 63
25
BmgBI CAC'GTC
Cuts at: 261 324 3148 261
Size: 63 2824 4618
Fragments arranged by size:
4618 2824 63
BmrI ACTGGGnnnn_n'
Cuts at: 4016 4717 4798 5133 7277 4016
Size: 701 81 335 2144 4244
Fragments arranged by size:
4244 2144 701 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5872 5872
Size: 7505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1067 1517 1895 1913 1976 2045 2120 2147
Size: 306 450 378 18 63 69 75 27
Cuts at: 2147 2273 2507 2705 3092 3439 3524 4261 761
Size: 126 234 198 387 347 85 737 4005
Fragments arranged by size:
4005 737 450 387 378 347 306 234
198 126 85 75 69 63 27 18
Bpu10I CC'TnA_GC
Cuts at: 1160 1448 1961 2231 3820 1160
Size: 288 513 270 1589 4845
Fragments arranged by size:
4845 1589 513 288 270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3707 3796 3877 4243 4681 5560 6274 6572
Size: 762 89 81 366 438 879 714 298
Cuts at: 6572 2945
Size: 3878
Fragments arranged by size:
3878 879 762 714 438 366 298 89
81
BsaI GGTCTCn'nnnn_
Cuts at: 331 676 2044 2096 2501 3214 3583 3991 7209
Size: 345 1368 52 405 713 369 408 3218
Cuts at: 7209 331
Size: 627
Fragments arranged by size:
3218 1368 713 627 408 405 369 345
52
BsaAI yAC'GTr
Cuts at: 4858 4858
Size: 7505
BsaBI GATnn'nnATC
Cuts at: 6 4297 5019 7056 6
Size: 4291 722 2037 455
Fragments arranged by size:
4291 2037 722 455
BsaHI Gr'CG_yC
Cuts at: 356 434 2111 2273 2390 2859 3200 3353 4936
Size: 78 1677 162 117 469 341 153 1583
Cuts at: 4936 5184 5886 7382 356
Size: 248 702 1496 479
Fragments arranged by size:
1677 1583 1496 702 479 469 341 248
162 153 117 78
BsaWI w'CCGG_w
Cuts at: 622 3842 4363 4694 5215 5893 6304 6451 6725
Size: 3220 521 331 521 678 411 147 274
Cuts at: 6725 622
Size: 1402
Fragments arranged by size:
3220 1402 678 521 521 411 331 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 1529 3023 4264 130
Size: 1399 1494 1241 3371
Fragments arranged by size:
3371 1494 1399 1241
BseYI C'CCAG_C
Cuts at: 590 2464 3682 3713 4930 5688 6353 6761 590
Size: 1874 1218 31 1217 758 665 408 1334
Fragments arranged by size:
1874 1334 1218 1217 758 665 408 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 4335 2330
Size: 2005 5500
BsiEI CG_ry'CG
Cuts at: 104 3615 5047 5093 6323 104
Size: 3511 1432 46 1230 1286
Fragments arranged by size:
3511 1432 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 693 864 999 1071 1314 1332 1422 1521 1962
Size: 171 135 72 243 18 90 99 441
Cuts at: 1962 2214 2259 2493 2682 2700 2943 3249 3258
Size: 252 45 234 189 18 243 306 9
Cuts at: 3258 3555 4519 5297 5487 6347 693
Size: 297 964 778 190 860 1851
Fragments arranged by size:
1851 964 860 778 441 306 297 252
243 243 234 190 189 171 135 99
90 72 45 18 18 9
BsmI GAATG_Cn'
Cuts at: 2297 2297
Size: 7505
BsmBI CGTCTCn'nnnn_
Cuts at: 251 3179 251
Size: 2928 4577
Bsp1286I G_dGCh'C
Cuts at: 278 693 864 999 1015 1053 1071 1106 1187
Size: 415 171 135 16 38 18 35 81
Cuts at: 1187 1314 1332 1350 1375 1422 1521 1827 1883
Size: 127 18 18 25 47 99 306 56
Cuts at: 1883 1962 2214 2250 2259 2439 2493 2502 2682
Size: 79 252 36 9 180 54 9 180
Cuts at: 2682 2700 2943 3112 3159 3249 3258 3267 3555
Size: 18 243 169 47 90 9 9 288
Cuts at: 3555 3599 3850 4135 4285 4455 4519 5130 5223
Size: 44 251 285 150 170 64 611 93
Cuts at: 5223 5297 5487 5549 6347 278
Size: 74 190 62 798 1436
Fragments arranged by size:
1436 798 611 415 306 288 285 252
251 243 190 180 180 171 170 169
150 135 127 99 93 90 81 79
74 64 62 56 54 47 47 44
38 36 35 25 18 18 18 18
16 9 9 9 9
BspEI T'CCGG_A
Cuts at: 3842 3842
Size: 7505
BspHI T'CATG_A
Cuts at: 5937 5937
Size: 7505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 232 3889 4009 4555 4769 5071 5452 232
Size: 3657 120 546 214 302 381 2285
Fragments arranged by size:
3657 2285 546 381 302 214 120
BsrBI CCG'CTC
Cuts at: 50 5797 5851 7335 7500 50
Size: 5747 54 1484 165 55
Fragments arranged by size:
5747 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5417 5417
Size: 7505
BsrFI r'CCGG_y
Cuts at: 430 1117 1783 2404 3223 3331 5503 5684 430
Size: 687 666 621 819 108 2172 181 2251
Fragments arranged by size:
2251 2172 819 687 666 621 181 108
BssSI C'ACGA_G
Cuts at: 665 1070 2492 4353 5776 6484 665
Size: 405 1422 1861 1423 708 1686
Fragments arranged by size:
1861 1686 1423 1422 708 405
BstAPI GCAn_nnn'nTGC
Cuts at: 4882 4882
Size: 7505
BstBI TT'CG_AA
Cuts at: 5866 5866
Size: 7505
BstEII G'GTnAC_C
Cuts at: 3208 3433 3208
Size: 225 7280
BstXI CCAn_nnnn'nTGG
Cuts at: 4249 4249
Size: 7505
BstYI r'GATC_y
Cuts at: 1 284 1669 2911 4298 4530 4997 5020 5355
Size: 283 1385 1242 1387 232 467 23 335
Cuts at: 5355 5601 5907 5919 6005 7057 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1387 1385 1242 1052 467 449 335 306
283 246 232 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 241 854 962 2330 241
Size: 613 108 1368 5416
Fragments arranged by size:
5416 1368 613 108
BtgI C'CryG_G
Cuts at: 647 755 1017 1584 1827 2394 2511 2835 3104
Size: 108 262 567 243 567 117 324 269
Cuts at: 3104 3213 3357 3384 3438 647
Size: 109 144 27 54 4714
Fragments arranged by size:
4714 567 567 324 269 262 243 144
117 109 108 54 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 332 2630 5455 5636 5799 332
Size: 2298 2825 181 163 2038
Fragments arranged by size:
2825 2298 2038 181 163
BtsI GCAGTG_nn'
Cuts at: 4212 4748 4212
Size: 536 6969
ClaI AT'CG_AT
Cuts at: 4427 4427
Size: 7505
DraI TTT'AAA
Cuts at: 4504 4504
Size: 7505
DraIII CAC_nnn'GTG
Cuts at: 1822 1822
Size: 7505
DrdI GACnn_nn'nnGTC
Cuts at: 3575 3665 5211 6555 3575
Size: 90 1546 1344 4525
Fragments arranged by size:
4525 1546 1344 90
EaeI y'GGCC_r
Cuts at: 149 171 495 2568 2748 3744 4125 4151 5090
Size: 22 324 2073 180 996 381 26 939
Cuts at: 5090 5264 5655 5682 7027 149
Size: 174 391 27 1345 627
Fragments arranged by size:
2073 1345 996 939 627 391 381 324
180 174 27 26 22
EagI C'GGCC_G
Cuts at: 5090 5090
Size: 7505
EarI CTCTTCn'nnn_
Cuts at: 3910 5528 5738 3910
Size: 1618 210 5677
Fragments arranged by size:
5677 1618 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5713 6455 6601 7315 5713
Size: 742 146 714 5903
Fragments arranged by size:
5903 742 714 146
EcoICRI GAG'CTC
Cuts at: 997 1519 1960 2257 2491 4517 997
Size: 522 441 297 234 2026 3985
Fragments arranged by size:
3985 2026 522 441 297 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 761 1067 1217 1517 1895 1913 1976 2045
Size: 30 306 150 300 378 18 63 69
Cuts at: 2045 2120 2147 2269 2273 2507 2705 3092 3439
Size: 75 27 122 4 234 198 387 347
Cuts at: 3439 3524 3684 4261 5330 5762 6115 6777 731
Size: 85 160 577 1069 432 353 662 1459
Fragments arranged by size:
1459 1069 662 577 432 387 378 353
347 306 300 234 198 160 150 122
85 75 69 63 30 27 18 4
EcoNI CCTnn'n_nnAGG
Cuts at: 4261 4261
Size: 7505
EcoO109I rG'GnC_Cy
Cuts at: 442 478 487 652 715 751 760 769 778
Size: 36 9 165 63 36 9 9 9
Cuts at: 778 1012 1103 1183 1184 1201 1354 1371 1372
Size: 234 91 80 1 17 153 17 1
Cuts at: 1372 1480 1624 1966 2011 2173 2326 2830 3064
Size: 108 144 342 45 162 153 504 234
Cuts at: 3064 3109 3379 3460 3496 3532 3596 4266 4644
Size: 45 270 81 36 36 64 670 378
Cuts at: 4644 442
Size: 3303
Fragments arranged by size:
3303 670 504 378 342 270 234 234
165 162 153 153 144 108 91 81
80 64 63 45 45 36 36 36
36 17 17 9 9 9 9 1
1
EcoRI G'AATT_C
Cuts at: 4465 4509 4465
Size: 44 7461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5524 5524
Size: 7505
FspI TGC'GCA
Cuts at: 5286 5286
Size: 7505
HaeII r_GCGC'y
Cuts at: 359 1925 2114 2276 2711 3203 3356 3689 4939
Size: 1566 189 162 435 492 153 333 1250
Cuts at: 4939 5187 6417 7188 359
Size: 248 1230 771 676
Fragments arranged by size:
1566 1250 1230 771 676 492 435 333
248 189 162 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 601 633 2896 2928 3285 3317 5347
Size: 32 78 32 2263 32 357 32 2030
Cuts at: 5347 5377 5379 5409 491
Size: 30 2 30 2587
Fragments arranged by size:
2587 2263 2030 357 78 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4544 4544
Size: 7505
KasI G'GCGC_C
Cuts at: 355 2110 2272 3199 3352 4935 5183 355
Size: 1755 162 927 153 1583 248 2677
Fragments arranged by size:
2677 1755 1583 927 248 162 153
KpnI G_GTAC'C
Cuts at: 249 4525 249
Size: 4276 3229
MluI A'CGCG_T
Cuts at: 6938 6938
Size: 7505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4160 4310 6264 6448 4160
Size: 150 1954 184 5217
Fragments arranged by size:
5217 1954 184 150
MscI TGG'CCA
Cuts at: 4153 5266 4153
Size: 1113 6392
MslI CAynn'nnrTG
Cuts at: 3740 4429 5494 5621 3740
Size: 689 1065 127 5624
Fragments arranged by size:
5624 1065 689 127
MspA1I CmG'CkG
Cuts at: 594 883 1126 1406 1630 1990 2314 2468 2647
Size: 289 243 280 224 360 324 154 179
Cuts at: 2647 3232 4161 4616 4930 5290 6074 6319 594
Size: 585 929 455 314 360 784 245 1780
Fragments arranged by size:
1780 929 784 585 455 360 360 324
314 289 280 245 243 224 179 154
NaeI GCC'GGC
Cuts at: 5686 5686
Size: 7505
NarI GG'CG_CC
Cuts at: 356 2111 2273 3200 3353 4936 5184 356
Size: 1755 162 927 153 1583 248 2677
Fragments arranged by size:
2677 1755 1583 927 248 162 153
NcoI C'CATG_G
Cuts at: 647 755 3104 647
Size: 108 2349 5048
Fragments arranged by size:
5048 2349 108
NgoMIV G'CCGG_C
Cuts at: 5684 5684
Size: 7505
NspI r_CATG'y
Cuts at: 4078 4558 5058 6661 4078
Size: 480 500 1603 4922
Fragments arranged by size:
4922 1603 500 480
PasI CC'CwG_GG
Cuts at: 1206 1368 1206
Size: 162 7343
PciI A'CATG_T
Cuts at: 6657 6657
Size: 7505
PfoI T'CCnGG_A
Cuts at: 528 555 1908 2097 2682 3654 528
Size: 27 1353 189 585 972 4379
Fragments arranged by size:
4379 1353 972 585 189 27
PmeI GTTT'AAAC
Cuts at: 4504 4504
Size: 7505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5951 5983 5951
Size: 32 7473
PpuMI rG'GwC_Cy
Cuts at: 442 478 652 715 751 760 769 778 1201
Size: 36 174 63 36 9 9 9 423
Cuts at: 1201 1480 1624 1966 2173 2830 3064 3460 3496
Size: 279 144 342 207 657 234 396 36
Cuts at: 3496 3532 442
Size: 36 4415
Fragments arranged by size:
4415 657 423 396 342 279 234 207
174 144 63 36 36 36 36 9
9 9
PspOMI G'GGCC_C
Cuts at: 1011 1102 1183 1371 3108 3595 1011
Size: 91 81 188 1737 487 4921
Fragments arranged by size:
4921 1737 487 188 91 81
PspXI vC'TCGA_Gb
Cuts at: 863 1520 3793 863
Size: 657 2273 4575
Fragments arranged by size:
4575 2273 657
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4192 4224 4192
Size: 32 7473
PstI C_TGCA'G
Cuts at: 4552 5237 4552
Size: 685 6820
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7505
PvuII CAG'CTG
Cuts at: 594 883 2314 4161 4930 5290 594
Size: 289 1431 1847 769 360 2809
Fragments arranged by size:
2809 1847 1431 769 360 289
RsrII CG'GwC_CG
Cuts at: 5700 5700
Size: 7505
SacI G_AGCT'C
Cuts at: 999 1521 1962 2259 2493 4519 999
Size: 522 441 297 234 2026 3985
Fragments arranged by size:
3985 2026 522 441 297 234
SalI G'TCGA_C
Cuts at: 4542 4542
Size: 7505
SanDI GG'GwC_CC
Cuts at: 442 478 652 760 1624 1966 3460 442
Size: 36 174 108 864 342 1494 4487
Fragments arranged by size:
4487 1494 864 342 174 108 36
SapI GCTCTTCn'nnn_
Cuts at: 5528 5738 5528
Size: 210 7295
SbfI CC_TGCA'GG
Cuts at: 4552 4552
Size: 7505
ScaI AGT'ACT
Cuts at: 3928 7022 3928
Size: 3094 4411
SexAI A'CCwGG_T
Cuts at: 693 819 1413 1575 1818 2079 2574 2592 693
Size: 126 594 162 243 261 495 18 5606
Fragments arranged by size:
5606 594 495 261 243 162 126 18
SfcI C'TryA_G
Cuts at: 33 4329 4548 5233 6201 6392 33
Size: 4296 219 685 968 191 1146
Fragments arranged by size:
4296 1146 968 685 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 467 494 849 1019 1235 1379 1658 3278 467
Size: 27 355 170 216 144 279 1620 4694
Fragments arranged by size:
4694 1620 355 279 216 170 144 27
SfoI GGC'GCC
Cuts at: 357 2112 2274 3201 3354 4937 5185 357
Size: 1755 162 927 153 1583 248 2677
Fragments arranged by size:
2677 1755 1583 927 248 162 153
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7505
SgrAI Cr'CCGG_yG
Cuts at: 430 430
Size: 7505
SmaI CCC'GGG
Cuts at: 2955 4527 2955
Size: 1572 5933
SmlI C'TyrA_G
Cuts at: 863 1520 2960 3722 3793 3811 3892 4258 4660
Size: 657 1440 762 71 18 81 366 402
Cuts at: 4660 5575 6289 6551 863
Size: 915 714 262 1817
Fragments arranged by size:
1817 1440 915 762 714 657 402 366
262 81 71 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7505
SphI G_CATG'C
Cuts at: 4558 5058 4558
Size: 500 7005
StuI AGG'CCT
Cuts at: 4192 4192
Size: 7505
StyI C'CwwG_G
Cuts at: 446 472 647 702 711 755 935 1179 1394
Size: 26 175 55 9 44 180 244 215
Cuts at: 1394 1619 1718 2106 3104 3203 3375 3945 4061
Size: 225 99 388 998 99 172 570 116
Cuts at: 4061 4439 4639 4993 446
Size: 378 200 354 2958
Fragments arranged by size:
2958 998 570 388 378 354 244 225
215 200 180 175 172 116 99 99
55 44 26 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 269 426 1918 2746 4114 5088 5221 5680 5889
Size: 157 1492 828 1368 974 133 459 209
Cuts at: 5889 269
Size: 1885
Fragments arranged by size:
1885 1492 1368 974 828 459 209 157
133
TatI w'GTAC_w
Cuts at: 3926 7020 3926
Size: 3094 4411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 620 652 7022 7054 620
Size: 32 6370 32 1071
Fragments arranged by size:
6370 1071 32 32
Tth111I GACn'n_nGTC
Cuts at: 5302 5302
Size: 7505
XbaI T'CTAG_A
Cuts at: 4536 4536
Size: 7505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 598 718 1213 2041 2275 3382 3463 4117 4428
Size: 120 495 828 234 1107 81 654 311
Cuts at: 4428 5000 598
Size: 572 3103
Fragments arranged by size:
3103 1107 828 654 572 495 311 234
120 81
XhoI C'TCGA_G
Cuts at: 863 1520 3793 863
Size: 657 2273 4575
Fragments arranged by size:
4575 2273 657
XmaI C'CCGG_G
Cuts at: 2953 4525 2953
Size: 1572 5933
ZraI GAC'GTC
Cuts at: 2860 2860
Size: 7505
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsaXI BsiWI BsrGI BssHII
BstZ17I CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI SacII SnaBI SrfI SspI SwaI XmnI