Restriction map for pF1KB4626
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvrII       BcgI        BciVI       
BfrI        BlnI        BsaBI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FseI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XcmI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AspI          289  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1217  TGCGCA               TGC^GCA
BalI          806  TGGCCA               TGG^CCA
BamHI        1691  GGATCC               G^GATCC
BbrPI         514  CACGTG               CAC^GTG
BclI         1055  TGATCA               T^GATCA
BglII         760  AGATCT               A^GATCT
BlpI          837  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      837  GCTCAGC              GC^T[ACGT]AGC
BsaAI         514  CACGTG               [CT]AC^GT[GA]
BsaI         1925  GAGACC               GGTCTC(1/5)
BsiEI         264  CGGCCG               CG[GA][CT]^CG
BspHI        1355  TCATGA               T^CATGA
BspLU11I      442  ACATGT               A^CATGT
BssSI        1776  CACGAG               CACGAG(-5/-1)
CelII         837  GCTCAGC              GC^T[ACGT]AGC
EagI          264  CGGCCG               C^GGCCG
EclXI         264  CGGCCG               C^GGCCG
Eco52I        264  CGGCCG               C^GGCCG
FbaI         1055  TGATCA               T^GATCA
FspI         1217  TGCGCA               TGC^GCA
HpaI          976  GTTAAC               GTT^AAC
MluNI         806  TGGCCA               TGG^CCA
MscI          806  TGGCCA               TGG^CCA
NaeI          266  GCCGGC               GCC^GGC
NgoMIV        266  GCCGGC               G^CCGGC
PflFI         289  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         514  CACGTG               CAC^GTG
PmlI          514  CACGTG               CAC^GTG
PpuMI        1838  GGGACCC              [GA]G^G[AT]CC[CT]
RcaI         1355  TCATGA               T^CATGA
SgrAI        1224  CACCGGCG             C[GA]^CCGG[CT]G
StuI         1541  AGGCCT               AGG^CCT
TfiI         1002  GAATC                G^A[AT]TC
Tsp509I       803  AATT                 ^AATT
Tth111I       289  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1872  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII       1388  TCCGGA               T^CCGGA
             1694  TCCGGA              
AfaI          330  GTAC                 GT^AC
             1302  GTAC                
AflIII        442  ACATGT               A^C[GA][CT]GT
              872  ACGTGT              
ApaI         1247  GGGCCC               GGGCC^C
             1587  GGGCCC              
BbvCI         396  CCTCAGC              CCTCAGC(-5/-2)
             1712  CCTCAGC             
BglI          619  GCCAGGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1446  GCCTCCAGGGC         
BseAI        1388  TCCGGA               T^CCGGA
             1694  TCCGGA              
BseRI        1263  CTCCTC               GAGGAG(10/8)
             1734  CTCCTC              
BspEI        1388  TCCGGA               T^CCGGA
             1694  TCCGGA              
BsrBI          20  GAGCGG               CCGCTC(-3/-3)
              261  GAGCGG              
BstAPI        183  GCAGATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              231  GCACTCCCTGC         
BstEII       1980  GGTCACC              G^GT[ACGT]ACC
             2280  GGTCACC             
BstPI        1980  GGTCACC              G^GT[ACGT]ACC
             2280  GGTCACC             
BstXI        1794  CCACGGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2270  CCAAGCAGCTGG        
EcoO65I      1980  GGTCACC              G^GT[ACGT]ACC
             2280  GGTCACC             
HincII        976  GTTAAC               GT[CT]^[GA]AC
             1363  GTCAAC              
HindII        976  GTTAAC               GT[CT]^[GA]AC
             1363  GTCAAC              
HindIII       355  AAGCTT               A^AGCTT
             1783  AAGCTT              
MroI         1388  TCCGGA               T^CCGGA
             1694  TCCGGA              
NcoI          866  CCATGG               C^CATGG
             1502  CCATGG              
NspI          442  ACATGT               [GA]CATG^[CT]
              776  GCATGT              
PaeR7I        414  CTCGAG               C^TCGAG
             1126  CTCGAG              
PflMI         333  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              636  CCATGAGCTGG         
RsaI          330  GTAC                 GT^AC
             1302  GTAC                
SmlI          414  CTCGAG               C^T[CT][GA]AG
             1126  CTCGAG              
Van91I        333  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              636  CCATGAGCTGG         
XhoI          414  CTCGAG               C^TCGAG
             1126  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        Alw44I      
AlwI        AlwNI       ApaLI       AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BsaWI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        StyI        TaqI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
XhoII       
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