Restriction map for pF1KB4613
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrDI       BsrFI       BsrGI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MaeI        
MamI        MboII       MfeI        MflI        MluI        
MroI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          741  GTAC                 GT^AC
Alw44I         55  GTGCAC               G^TGCAC
AlwNI         112  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          55  GTGCAC               G^TGCAC
AspHI          55  GTGCAC               G[AT]GC[AT]^C
AvaI          480  CTCGGG               C^[CT]CG[GA]G
BalI          213  TGGCCA               TGG^CCA
BanII         566  GGGCTC               G[GA]GC[CT]^C
BbeI          727  GGCGCC               GGCGC^C
BglI          215  GCCACCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI         463  GAGGAG               GAGGAG(10/8)
BsiEI           5  CGACCG               CG[GA][CT]^CG
BsiHKAI        55  GTGCAC               G[AT]GC[AT]^C
BsmAI         681  GAGAC                GTCTC(1/5)
BsoBI         480  CTCGGG               C^[CT]CG[GA]G
BspLU11I      425  ACATGT               A^CATGT
BspMI         392  GCAGGT               ACCTGC(4/8)
BsrBI         160  CCGCTC               CCGCTC(-3/-3)
BssHII        224  GCGCGC               G^CGCGC
BssSI         492  CACGAG               CACGAG(-5/-1)
DraII         401  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        772  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI            6  GACCGCAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          771  CCACGG               C^C[GA][CT]GG
EaeI          213  TGGCCA               [CT]^GGCC[GA]
EcoO109I      401  AGGCCCT              [GA]G^G[ACGT]CC[CT]
KasI          727  GGCGCC               G^GCGCC
MaeIII        674  GTAAC                ^GT[ACGT]AC
MluNI         213  TGGCCA               TGG^CCA
MscI          213  TGGCCA               TGG^CCA
MslI          546  CATCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          727  GGCGCC               GG^CGCC
PshAI         783  GACGCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          741  GTAC                 GT^AC
SfoI          727  GGCGCC               GGC^GCC
SphI           77  GCATGC               GCATG^C
TaqI          548  TCGA                 T^CGA
TspRI         178  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       548  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          727  GGCGCC               G[GA]^CG[CT]C
              783  GACGCC              
AflIII        368  ACGTGT               A^C[GA][CT]GT
              425  ACATGT              
AlwI          182  GGATC                GGATC(4/5)
              308  GGATC               
AvaII         205  GGTCC                G^G[AT]CC
              539  GGACC               
BanI          352  GGCACC               G^G[CT][GA]CC
              727  GGCGCC              
BlpI          162  GCTCAGC              GC^T[ACGT]AGC
              411  GCTGAGC             
Bpu1102I      162  GCTCAGC              GC^T[ACGT]AGC
              411  GCTGAGC             
BsaHI         727  GGCGCC               G[GA]^CG[CT]C
              783  GACGCC              
BsgI          121  GTGCAG               GTGCAG(16/14)
              778  GTGCAG              
BsmFI         715  GGGAC                GGGAC(10/14)
              755  GGGAC               
BsrI          315  ACTGG                ACTGG(1/-1)
              177  CCAGT               
BstF5I        698  GGATG                GGATG(2/0)
              735  GGATG               
CelII         162  GCTCAGC              GC^T[ACGT]AGC
              411  GCTGAGC             
DdeI          163  CTCAG                C^T[ACGT]AG
              412  CTGAG               
EcoNI         117  CCTGGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              706  CCTCCCGGAGG         
FokI          698  GGATG                GGATG(9/13)
              735  GGATG               
Hin1I         727  GGCGCC               G[GA]^CG[CT]C
              783  GACGCC              
HinfI         472  GACTC                G^A[ACGT]TC
              717  GACTC               
MaeII         335  ACGT                 A^CGT
              368  ACGT                
NlaIII         78  CATG                 CATG^
              426  CATG                
NspI           77  GCATGC               [GA]CATG^[CT]
              425  ACATGT              
PleI          472  GACTC                GAGTC(4/5)
              717  GACTC               
PvuII         409  CAGCTG               CAG^CTG
              640  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DpnII       EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MboI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIV       
PstI        Sau3AI      Sau96I      ScrFI       SfcI        
TseI        
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