Restriction map for pF1KB4592
 (Circular) (Six-base) MAPSORT of: pF1KB4592.seq  Check: 2833  from: 1  to: 4916



pF1KB4592  4916 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AccI GT'mk_AC

Cuts at:   1201    1508    1954    1201 
   Size:        307     446    4163

  Fragments arranged by size: 

                4163     446     307
 
Acc65I G'GTAC_C

Cuts at:   1932    1932 
   Size:       4916
 
AclI AA'CG_TT

Cuts at:   4756    4756 
   Size:       4916
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    767    1052    2741    3173    3526    4188     767 
   Size:        285    1689     432     353     662    1495

  Fragments arranged by size: 

                1689    1495     662     432     353     285
 
AflIII A'CryG_T

Cuts at:   1161    2268    4068    4349    1161 
   Size:       1107    1800     281    1728

  Fragments arranged by size: 

                1800    1728    1107     281
 
AlwNI CAG_nnn'CTG

Cuts at:   1048    2221    3659    1048 
   Size:       1173    1438    2305

  Fragments arranged by size: 

                2305    1438    1173
 
ApaI G_GGCC'C

Cuts at:   1578    1578 
   Size:       4916
 
ApaLI G'TGCA_C

Cuts at:   3754    3754 
   Size:       4916
 
ApoI r'AATT_y

Cuts at:    366     715    1118    1453    1473    1920    4269    4525     366 
   Size:        349     403     335      20     447    2349     256     757

  Fragments arranged by size: 

                2349     757     447     403     349     335     256      20
            
 
AseI AT'TA_AT

Cuts at:     20    1658      20 
   Size:       1638    3278
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4916
 
AvaI C'yCGr_G

Cuts at:    127    1936     127 
   Size:       1809    3107
 
BamHI G'GATC_C

Cuts at:   1941    1941 
   Size:       4916
 
BanI G'GyrC_C

Cuts at:   1932    2346    2594    2629    1932 
   Size:        414     248      35    4219

  Fragments arranged by size: 

                4219     414     248      35
 
BanII G_rGCy'C

Cuts at:    128     203     953    1578    1930    2960     128 
   Size:         75     750     625     352    1030    2084

  Fragments arranged by size: 

                2084    1030     750     625     352      75
 
BbeI G_GCGC'C

Cuts at:   2350    2598    2350 
   Size:        248    4668
 
BbsI GAAGACnn'nnnn_

Cuts at:    218    1862     218 
   Size:       1644    3272
 
BciVI GTATCCnnnnn_n'

Cuts at:   1155    2814    3870    4916    1155 
   Size:       1659    1056    1046    1155

  Fragments arranged by size: 

                1659    1155    1056    1046
 
BclI T'GATC_A

Cuts at:   2436    2436 
   Size:       4916
 
BglII A'GATC_T

Cuts at:    485    2431    4468     485 
   Size:       1946    2037     933

  Fragments arranged by size: 

                2037    1946     933
 
BlpI GC'TnA_GC

Cuts at:    353     546    2028     353 
   Size:        193    1482    3241

  Fragments arranged by size: 

                3241    1482     193
 
Bme1580I G_kGCm'C

Cuts at:   1578    2541    2634    3758    1578 
   Size:        963      93    1124    2736

  Fragments arranged by size: 

                2736    1124     963      93
 
BmgBI CAC'GTC

Cuts at:    216     691     216 
   Size:        475    4441
 
BmrI ACTGGGnnnn_n'

Cuts at:    834    2128    2209    2544    4688     834 
   Size:       1294      81     335    2144    1062

  Fragments arranged by size: 

                2144    1294    1062     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3283    3283 
   Size:       4916
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    937     937 
   Size:       4916
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    883    2092    2971    3685    3983     883 
   Size:       1209     879     714     298    1816

  Fragments arranged by size: 

                1816    1209     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1093    1727    4620    1093 
   Size:        634    2893    1389

  Fragments arranged by size: 

                2893    1389     634
 
BsaAI yAC'GTr

Cuts at:   2269    2269 
   Size:       4916
 
BsaBI GATnn'nnATC

Cuts at:      6    1154    1330    2430    4467       6 
   Size:       1148     176    1100    2037     455

  Fragments arranged by size: 

                2037    1148    1100     455     176
 
BsaHI Gr'CG_yC

Cuts at:   2347    2595    3297    4793    2347 
   Size:        248     702    1496    2470

  Fragments arranged by size: 

                2470    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   2105    2626    3304    3715    3862    4136    2105 
   Size:        521     678     411     147     274    2885

  Fragments arranged by size: 

                2885     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1724    1754    1724 
   Size:         30    4886
 
BseYI C'CCAG_C

Cuts at:    360    1443    2341    3099    3764    4172     360 
   Size:       1083     898     758     665     408    1104

  Fragments arranged by size: 

                1104    1083     898     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    238     238 
   Size:       4916
 
BsiEI CG_ry'CG

Cuts at:    104     182    2458    2504    3734     104 
   Size:         78    2276      46    1230    1286

  Fragments arranged by size: 

                2276    1286    1230      78      46
 
BsiHKAI G_wGCw'C

Cuts at:    128     203     667     953    1930    2708    2898    3758     128 
   Size:         75     464     286     977     778     190     860    1286

  Fragments arranged by size: 

                1286     977     860     778     464     286     190      75
            
 
Bsp1286I G_dGCh'C

Cuts at:    128     203     667     953    1578    1930    2541    2634    2708 
   Size:         75     464     286     625     352     611      93      74

Cuts at:   2708    2898    2960    3758     128 
   Size:        190      62     798    1286

  Fragments arranged by size: 

                1286     798     625     611     464     352     286     190
                  93      75      74      62
 
BspHI T'CATG_A

Cuts at:   1541    3348    1541 
   Size:       1807    3109
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1966    2180    2482    2863    1966 
   Size:        214     302     381    4019

  Fragments arranged by size: 

                4019     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3208    3262    4746    4911      50 
   Size:       3158      54    1484     165      55

  Fragments arranged by size: 

                3158    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    213    2828     213 
   Size:       2615    2301
 
BsrFI r'CCGG_y

Cuts at:    133    2914    3095     133 
   Size:       2781     181    1954

  Fragments arranged by size: 

                2781    1954     181
 
BssSI C'ACGA_G

Cuts at:   1495    3187    3895    1495 
   Size:       1692     708    2516

  Fragments arranged by size: 

                2516    1692     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    622    1048    1384    1707    2293     622 
   Size:        426     336     323     586    3245

  Fragments arranged by size: 

                3245     586     426     336     323
 
BstBI TT'CG_AA

Cuts at:   3277    3277 
   Size:       4916
 
BstYI r'GATC_y

Cuts at:      1     485    1941    2408    2431    2766    3012    3318    3330 
   Size:        484    1456     467      23     335     246     306      12

Cuts at:   3330    3416    4468       1 
   Size:         86    1052     449

  Fragments arranged by size: 

                1456    1052     484     467     449     335     306     246
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:   1509    1509 
   Size:       4916
 
BtgI C'CryG_G

Cuts at:    107     962     107 
   Size:        855    4061
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2866    3047    3210    2866 
   Size:        181     163    4572

  Fragments arranged by size: 

                4572     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2159    2159 
   Size:       4916
 
DraI TTT'AAA

Cuts at:   1915    1915 
   Size:       4916
 
DrdI GACnn_nn'nnGTC

Cuts at:   1864    2622    3966    1864 
   Size:        758    1344    2814

  Fragments arranged by size: 

                2814    1344     758
 
EaeI y'GGCC_r

Cuts at:   1406    1763    2501    2675    3066    3093    4438    1406 
   Size:        357     738     174     391      27    1345    1884

  Fragments arranged by size: 

                1884    1345     738     391     357     174      27
 
EagI C'GGCC_G

Cuts at:   2501    2501 
   Size:       4916
 
EarI CTCTTCn'nnn_

Cuts at:    437    2939    3149     437 
   Size:       2502     210    2204

  Fragments arranged by size: 

                2502    2204     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1390    3124    3866    4012    4726    1390 
   Size:       1734     742     146     714    1580

  Fragments arranged by size: 

                1734    1580     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    126     201     951    1928     126 
   Size:         75     750     977    3114

  Fragments arranged by size: 

                3114     977     750      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    767     937    1052    2741    3173    3526    4188     767 
   Size:        170     115    1689     432     353     662    1495

  Fragments arranged by size: 

                1689    1495     662     432     353     170     115
 
EcoO109I rG'GnC_Cy

Cuts at:    281     388     967    2055     281 
   Size:        107     579    1088    3142

  Fragments arranged by size: 

                3142    1088     579     107
 
EcoRI G'AATT_C

Cuts at:   1453    1473    1920    1453 
   Size:         20     447    4449

  Fragments arranged by size: 

                4449     447      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2935    2935 
   Size:       4916
 
FspI TGC'GCA

Cuts at:   2697    2697 
   Size:       4916
 
HaeII r_GCGC'y

Cuts at:   2350    2598    3828    4599    2350 
   Size:        248    1230     771    2667

  Fragments arranged by size: 

                2667    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2758    2788    2790    2820    2758 
   Size:         30       2      30    4854

  Fragments arranged by size: 

                4854      30      30       2
 
HincII GTy'rAC

Cuts at:   1955    1955 
   Size:       4916
 
HindIII A'AGCT_T

Cuts at:    518     821     518 
   Size:        303    4613
 
KasI G'GCGC_C

Cuts at:   2346    2594    2346 
   Size:        248    4668
 
KpnI G_GTAC'C

Cuts at:   1936    1936 
   Size:       4916
 
MfeI C'AATT_G

Cuts at:    254     254 
   Size:       4916
 
MluI A'CGCG_T

Cuts at:   4349    4349 
   Size:       4916
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    249    3675    3859     249 
   Size:       3426     184    1306

  Fragments arranged by size: 

                3426    1306     184
 
MscI TGG'CCA

Cuts at:   1408    1765    2677    1408 
   Size:        357     912    3647

  Fragments arranged by size: 

                3647     912     357
 
MslI CAynn'nnrTG

Cuts at:    582     679    1108    2905    3032     582 
   Size:         97     429    1797     127    2466

  Fragments arranged by size: 

                2466    1797     429     127      97
 
MspA1I CmG'CkG

Cuts at:    148     360    1045    1428    1443    2027    2341    2701    3485 
   Size:        212     685     383      15     584     314     360     784

Cuts at:   3485    3730     148 
   Size:        245    1334

  Fragments arranged by size: 

                1334     784     685     584     383     360     314     245
                 212      15
 
NaeI GCC'GGC

Cuts at:   3097    3097 
   Size:       4916
 
NarI GG'CG_CC

Cuts at:   2347    2595    2347 
   Size:        248    4668
 
NcoI C'CATG_G

Cuts at:    107     962     107 
   Size:        855    4061
 
NgoMIV G'CCGG_C

Cuts at:   3095    3095 
   Size:       4916
 
NspI r_CATG'y

Cuts at:    587    1165    1969    2469    4072     587 
   Size:        578     804     500    1603    1431

  Fragments arranged by size: 

                1603    1431     804     578     500
 
PasI CC'CwG_GG

Cuts at:    611     611 
   Size:       4916
 
PciI A'CATG_T

Cuts at:   1161    4068    1161 
   Size:       2907    2009
 
PmeI GTTT'AAAC

Cuts at:   1915    1915 
   Size:       4916
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3362    3394    3362 
   Size:         32    4884
 
PpuMI rG'GwC_Cy

Cuts at:    281     388     967     281 
   Size:        107     579    4230

  Fragments arranged by size: 

                4230     579     107
 
PspOMI G'GGCC_C

Cuts at:   1574    1574 
   Size:       4916
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1223    1255    1733    1765    1223 
   Size:         32     478      32    4374

  Fragments arranged by size: 

                4374     478      32      32
 
PstI C_TGCA'G

Cuts at:   1963    2648    1963 
   Size:        685    4231
 
PvuI CG_AT'CG

Cuts at:    104     182     104 
   Size:         78    4838
 
PvuII CAG'CTG

Cuts at:    360    1045    1443    2341    2701     360 
   Size:        685     398     898     360    2575

  Fragments arranged by size: 

                2575     898     685     398     360
 
RsrII CG'GwC_CG

Cuts at:   3111    3111 
   Size:       4916
 
SacI G_AGCT'C

Cuts at:    128     203     953    1930     128 
   Size:         75     750     977    3114

  Fragments arranged by size: 

                3114     977     750      75
 
SalI G'TCGA_C

Cuts at:   1953    1953 
   Size:       4916
 
SapI GCTCTTCn'nnn_

Cuts at:   2939    3149    2939 
   Size:        210    4706
 
SbfI CC_TGCA'GG

Cuts at:   1963    1963 
   Size:       4916
 
ScaI AGT'ACT

Cuts at:   1832    4433    1832 
   Size:       2601    2315
 
SfcI C'TryA_G

Cuts at:     33    1834    1959    2644    3612    3803      33 
   Size:       1801     125     685     968     191    1146

  Fragments arranged by size: 

                1801    1146     968     685     191     125
 
SfoI GGC'GCC

Cuts at:   2348    2596    2348 
   Size:        248    4668
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4916
 
SmaI CCC'GGG

Cuts at:   1938    1938 
   Size:       4916
 
SmlI C'TyrA_G

Cuts at:    862    2071    2986    3700    3962     862 
   Size:       1209     915     714     262    1816

  Fragments arranged by size: 

                1816    1209     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67     772    1548      67 
   Size:        705     776    3435

  Fragments arranged by size: 

                3435     776     705
 
SphI G_CATG'C

Cuts at:   1969    2469    1969 
   Size:        500    4416
 
StuI AGG'CCT

Cuts at:   1675    1675 
   Size:       4916
 
StyI C'CwwG_G

Cuts at:    107     962    2050    2404     107 
   Size:        855    1088     354    2619

  Fragments arranged by size: 

                2619    1088     855     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    700    1435    2499    2632    3091    3300     700 
   Size:        735    1064     133     459     209    2316

  Fragments arranged by size: 

                2316    1064     735     459     209     133
 
TatI w'GTAC_w

Cuts at:   1830    4431    1830 
   Size:       2601    2315
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    273     305    4433    4465     273 
   Size:         32    4128      32     724

  Fragments arranged by size: 

                4128     724      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2713    2713 
   Size:       4916
 
XbaI T'CTAG_A

Cuts at:   1794    1947    1794 
   Size:        153    4763
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1371    2411    1371 
   Size:       1040    3876
 
XmaI C'CCGG_G

Cuts at:   1936    1936 
   Size:       4916
 
XmnI GAAnn'nnTTC

Cuts at:    758    1235     758 
   Size:        477    4439
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PmeI     PpiI    PpuMI   PspOMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
     BmtI   Bpu10I    BseRI    BsiWI     BsmI    BsmBI    BspEI    BsrGI
   BssHII   BstEII    BstXI   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     HpaI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmlI    PshAI     PsiI    PspXI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     ZraI
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