Restriction map for pF1KB4592
 (Linear) (Six-base) MAPSORT of: KIBB4592.seq  Check: 2101  from: 1  to: 1803



KIBB4592.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0    1093    1400    1803 
   Size:       1093     307     403

  Fragments arranged by size: 

                1093     403     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     659     944    1803 
   Size:        659     285     859

  Fragments arranged by size: 

                 859     659     285
 
AflIII A'CryG_T

Cuts at:      0    1053    1803 
   Size:       1053     750
 
AlwNI CAG_nnn'CTG

Cuts at:      0     940    1803 
   Size:        940     863
 
ApaI G_GGCC'C

Cuts at:      0    1470    1803 
   Size:       1470     333
 
ApoI r'AATT_y

Cuts at:      0     258     607    1010    1345    1365    1803 
   Size:        258     349     403     335      20     438

  Fragments arranged by size: 

                 438     403     349     335     258      20
 
AseI AT'TA_AT

Cuts at:      0    1550    1803 
   Size:       1550     253
 
AvaI C'yCGr_G

Cuts at:      0      19    1803 
   Size:         19    1784
 
BanII G_rGCy'C

Cuts at:      0      20      95     845    1470    1803 
   Size:         20      75     750     625     333

  Fragments arranged by size: 

                 750     625     333      75      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     110    1754    1803 
   Size:        110    1644      49

  Fragments arranged by size: 

                1644     110      49
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1047    1803 
   Size:       1047     756
 
BglII A'GATC_T

Cuts at:      0     377    1803 
   Size:        377    1426
 
BlpI GC'TnA_GC

Cuts at:      0     245     438    1803 
   Size:        245     193    1365

  Fragments arranged by size: 

                1365     245     193
 
Bme1580I G_kGCm'C

Cuts at:      0    1470    1803 
   Size:       1470     333
 
BmgBI CAC'GTC

Cuts at:      0     108     583    1803 
   Size:        108     475    1220

  Fragments arranged by size: 

                1220     475     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     726    1803 
   Size:        726    1077
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     829    1803 
   Size:        829     974
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     775    1803 
   Size:        775    1028
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     985    1619    1803 
   Size:        985     634     184

  Fragments arranged by size: 

                 985     634     184
 
BsaBI GATnn'nnATC

Cuts at:      0    1046    1222    1803 
   Size:       1046     176     581

  Fragments arranged by size: 

                1046     581     176
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1616    1646    1803 
   Size:       1616      30     157

  Fragments arranged by size: 

                1616     157      30
 
BseYI C'CCAG_C

Cuts at:      0     252    1335    1803 
   Size:        252    1083     468

  Fragments arranged by size: 

                1083     468     252
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130    1803 
   Size:        130    1673
 
BsiEI CG_ry'CG

Cuts at:      0      74    1803 
   Size:         74    1729
 
BsiHKAI G_wGCw'C

Cuts at:      0      20      95     559     845    1803 
   Size:         20      75     464     286     958

  Fragments arranged by size: 

                 958     464     286      75      20
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      95     559     845    1470    1803 
   Size:         20      75     464     286     625     333

  Fragments arranged by size: 

                 625     464     333     286      75      20
 
BspHI T'CATG_A

Cuts at:      0    1433    1803 
   Size:       1433     370
 
BsrDI GCAATG_nn'

Cuts at:      0     105    1803 
   Size:        105    1698
 
BsrFI r'CCGG_y

Cuts at:      0      25    1803 
   Size:         25    1778
 
BssSI C'ACGA_G

Cuts at:      0    1387    1803 
   Size:       1387     416
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     514     940    1276    1599    1803 
   Size:        514     426     336     323     204

  Fragments arranged by size: 

                 514     426     336     323     204
 
BstYI r'GATC_y

Cuts at:      0     377    1803 
   Size:        377    1426
 
BstZ17I GTA'TAC

Cuts at:      0    1401    1803 
   Size:       1401     402
 
BtgI C'CryG_G

Cuts at:      0     854    1803 
   Size:        854     949
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1756    1803 
   Size:       1756      47
 
EaeI y'GGCC_r

Cuts at:      0    1298    1655    1803 
   Size:       1298     357     148

  Fragments arranged by size: 

                1298     357     148
 
EarI CTCTTCn'nnn_

Cuts at:      0     329    1803 
   Size:        329    1474
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1282    1803 
   Size:       1282     521
 
EcoICRI GAG'CTC

Cuts at:      0      18      93     843    1803 
   Size:         18      75     750     960

  Fragments arranged by size: 

                 960     750      75      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     659     829     944    1803 
   Size:        659     170     115     859

  Fragments arranged by size: 

                 859     659     170     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0     173     280     859    1803 
   Size:        173     107     579     944

  Fragments arranged by size: 

                 944     579     173     107
 
EcoRI G'AATT_C

Cuts at:      0    1345    1365    1803 
   Size:       1345      20     438

  Fragments arranged by size: 

                1345     438      20
 
HindIII A'AGCT_T

Cuts at:      0     410     713    1803 
   Size:        410     303    1090

  Fragments arranged by size: 

                1090     410     303
 
MfeI C'AATT_G

Cuts at:      0     146    1803 
   Size:        146    1657
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     141    1803 
   Size:        141    1662
 
MscI TGG'CCA

Cuts at:      0    1300    1657    1803 
   Size:       1300     357     146

  Fragments arranged by size: 

                1300     357     146
 
MslI CAynn'nnrTG

Cuts at:      0     474     571    1000    1803 
   Size:        474      97     429     803

  Fragments arranged by size: 

                 803     474     429      97
 
MspA1I CmG'CkG

Cuts at:      0      40     252     937    1320    1335    1803 
   Size:         40     212     685     383      15     468

  Fragments arranged by size: 

                 685     468     383     212      40      15
 
NcoI C'CATG_G

Cuts at:      0     854    1803 
   Size:        854     949
 
NspI r_CATG'y

Cuts at:      0     479    1057    1803 
   Size:        479     578     746

  Fragments arranged by size: 

                 746     578     479
 
PasI CC'CwG_GG

Cuts at:      0     503    1803 
   Size:        503    1300
 
PciI A'CATG_T

Cuts at:      0    1053    1803 
   Size:       1053     750
 
PpuMI rG'GwC_Cy

Cuts at:      0     173     280     859    1803 
   Size:        173     107     579     944

  Fragments arranged by size: 

                 944     579     173     107
 
PspOMI G'GGCC_C

Cuts at:      0    1466    1803 
   Size:       1466     337
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1115    1147    1625    1657    1803 
   Size:       1115      32     478      32     146

  Fragments arranged by size: 

                1115     478     146      32      32
 
PvuI CG_AT'CG

Cuts at:      0      74    1803 
   Size:         74    1729
 
PvuII CAG'CTG

Cuts at:      0     252     937    1335    1803 
   Size:        252     685     398     468

  Fragments arranged by size: 

                 685     468     398     252
 
SacI G_AGCT'C

Cuts at:      0      20      95     845    1803 
   Size:         20      75     750     958

  Fragments arranged by size: 

                 958     750      75      20
 
ScaI AGT'ACT

Cuts at:      0    1724    1803 
   Size:       1724      79
 
SfcI C'TryA_G

Cuts at:      0    1726    1803 
   Size:       1726      77
 
SmlI C'TyrA_G

Cuts at:      0     754    1803 
   Size:        754    1049
 
SpeI A'CTAG_T

Cuts at:      0     664    1440    1803 
   Size:        664     776     363

  Fragments arranged by size: 

                 776     664     363
 
StuI AGG'CCT

Cuts at:      0    1567    1803 
   Size:       1567     236
 
StyI C'CwwG_G

Cuts at:      0     854    1803 
   Size:        854     949
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     592    1327    1803 
   Size:        592     735     476

  Fragments arranged by size: 

                 735     592     476
 
TatI w'GTAC_w

Cuts at:      0    1722    1803 
   Size:       1722      81
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     165     197    1803 
   Size:        165      32    1606

  Fragments arranged by size: 

                1606     165      32
 
XbaI T'CTAG_A

Cuts at:      0    1686    1803 
   Size:       1686     117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1263    1803 
   Size:       1263     540
 
XmnI GAAnn'nnTTC

Cuts at:      0     650    1127    1803 
   Size:        650     477     676

  Fragments arranged by size: 

                 676     650     477
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI     ApoI     AseI     AvaI
    BanII     BbsI    BciVI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrFI    BssSI   BstAPI    BstYI
  BstZ17I     BtgI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI  HindIII     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI     PciI    PpuMI   PspOMI     PsrI     PvuI
    PvuII     SacI     ScaI     SfcI     SmlI     SpeI     StuI     StyI
    TaqII     TatI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BseRI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII    BstBI
   BstEII    BstXI   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PstI    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     SwaI  Tth111I     XhoI     XmaI     ZraI
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