Restriction map for pF1KB4505
 (Circular) (Six-base) MAPSORT of: pF1KB4505.seq  Check: 3742  from: 1  to: 6629



pF1KB4505  6629 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    561     662    1177    1357    3228    3369     561 
   Size:        101     515     180    1871     141    3821

  Fragments arranged by size: 

                3821    1871     515     180     141     101
 
AatII G_ACGT'C

Cuts at:    533    1337     533 
   Size:        804    5825
 
AccI GT'mk_AC

Cuts at:   2561    3667    2561 
   Size:       1106    5523
 
Acc65I G'GTAC_C

Cuts at:   3645    3645 
   Size:       6629
 
AclI AA'CG_TT

Cuts at:   6469    6469 
   Size:       6629
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    696     709    2103    2967    3120    4454    4886    5633    5901 
   Size:         13    1394     864     153    1334     432     747     268

Cuts at:   5901     696 
   Size:       1424

  Fragments arranged by size: 

                1424    1394    1334     864     747     432     268     153
                  13
 
AflIII A'CryG_T

Cuts at:   1055    1126    1294    3981    5085    6062    1055 
   Size:         71     168    2687    1104     977    1622

  Fragments arranged by size: 

                2687    1622    1104     977     168      71
 
AgeI A'CCGG_T

Cuts at:    300    5017     300 
   Size:       4717    1912
 
AhdI GACnn_n'nnGTC

Cuts at:    390     390 
   Size:       6629
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2477    2477 
   Size:       6629
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1066    1098    1066 
   Size:         32    6597
 
AlwNI CAG_nnn'CTG

Cuts at:   3934    5501    3934 
   Size:       1567    5062
 
ApaI G_GGCC'C

Cuts at:    251     424     877    1361     251 
   Size:        173     453     484    5519

  Fragments arranged by size: 

                5519     484     453     173
 
ApaLI G'TGCA_C

Cuts at:   2452    2629    5399    2452 
   Size:        177    2770    3682

  Fragments arranged by size: 

                3682    2770     177
 
ApoI r'AATT_y

Cuts at:   1141    2280    3633    5982    6238    1141 
   Size:       1139    1353    2349     256    1532

  Fragments arranged by size: 

                2349    1532    1353    1139     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6629
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6629
 
AvaI C'yCGr_G

Cuts at:    895     948    1661    1785    3649     895 
   Size:         53     713     124    1864    3875

  Fragments arranged by size: 

                3875    1864     713     124      53
 
BamHI G'GATC_C

Cuts at:   1646    3009    3654    1646 
   Size:       1363     645    4621

  Fragments arranged by size: 

                4621    1363     645
 
BanI G'GyrC_C

Cuts at:    229     502    1570    1861    1926    3443    3645    4059    4307 
   Size:        273    1068     291      65    1517     202     414     248

Cuts at:   4307    4342     229 
   Size:         35    2516

  Fragments arranged by size: 

                2516    1517    1068     414     291     273     248     202
                  65      35
 
BanII G_rGCy'C

Cuts at:    251     424     641     877     947     956    1028    1361    1603 
   Size:        173     217     236      70       9      72     333     242

Cuts at:   1603    1794    2051    2174    3643    4673     251 
   Size:        191     257     123    1469    1030    2207

  Fragments arranged by size: 

                2207    1469    1030     333     257     242     236     217
                 191     173     123      72      70       9
 
BbeI G_GCGC'C

Cuts at:    233    1574    4063    4311     233 
   Size:       1341    2489     248    2551

  Fragments arranged by size: 

                2551    2489    1341     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1439    2300    2523    3561    1439 
   Size:        861     223    1038    4507

  Fragments arranged by size: 

                4507    1038     861     223
 
BbvCI CC'TCA_GC

Cuts at:    319     319 
   Size:       6629
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3160    3194    3160 
   Size:         34    6595
 
BciVI GTATCCnnnnn_n'

Cuts at:   4527    5288    6629    4527 
   Size:        761    1341    4527

  Fragments arranged by size: 

                4527    1341     761
 
BclI T'GATC_A

Cuts at:   4149    4149 
   Size:       6629
 
BglI GCCn_nnn'nGGC

Cuts at:    791    1844    2153    2758    3059     791 
   Size:       1053     309     605     301    4361

  Fragments arranged by size: 

                4361    1053     605     309     301
 
BglII A'GATC_T

Cuts at:   4144    6181    4144 
   Size:       2037    4592
 
BlpI GC'TnA_GC

Cuts at:    976    3741     976 
   Size:       2765    3864
 
Bme1580I G_kGCm'C

Cuts at:    251     403     424     877    1361    1514    1893    2456    2633 
   Size:        152      21     453     484     153     379     563     177

Cuts at:   2633    4254    4347    5403     251 
   Size:       1621      93    1056    1477

  Fragments arranged by size: 

                1621    1477    1056     563     484     453     379     177
                 153     152      93      21
 
BmgBI CAC'GTC

Cuts at:    960    1295    2652     960 
   Size:        335    1357    4937

  Fragments arranged by size: 

                4937    1357     335
 
BmrI ACTGGGnnnn_n'

Cuts at:   1275    1501    1788    3051    3264    3841    3922    4257    6401 
   Size:        226     287    1263     213     577      81     335    2144

Cuts at:   6401    1275 
   Size:       1503

  Fragments arranged by size: 

                2144    1503    1263     577     335     287     226     213
                  81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    471    4996     471 
   Size:       4525    2104
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    135     436     544    1446    1617     135 
   Size:        301     108     902     171    5147

  Fragments arranged by size: 

                5147     902     301     171     108
 
Bpu10I CC'TnA_GC

Cuts at:    319    1679     319 
   Size:       1360    5269
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    361     973    3286    3560    3586    3805    4684    5176    5474 
   Size:        612    2313     274      26     219     879     492     298

Cuts at:   5474     361 
   Size:       1516

  Fragments arranged by size: 

                2313    1516     879     612     492     298     274     219
                  26
 
BsaI GGTCTCn'nnnn_

Cuts at:    433     522     577    1265    1546    6333     433 
   Size:         89      55     688     281    4787     729

  Fragments arranged by size: 

                4787     729     688     281      89      55
 
BsaAI yAC'GTr

Cuts at:    600     681     870    1127    2451    3153    3982     600 
   Size:         81     189     257    1324     702     829    3247

  Fragments arranged by size: 

                3247    1324     829     702     257     189      81
 
BsaBI GATnn'nnATC

Cuts at:      6    4143    6180       6 
   Size:       4137    2037     455

  Fragments arranged by size: 

                4137    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    230     530    1334    1571    2498    2789    2819    4060    4308 
   Size:        300     804     237     927     291      30    1241     248

Cuts at:   4308    5010    6506     230 
   Size:        702    1496     353

  Fragments arranged by size: 

                1496    1241     927     804     702     353     300     291
                 248     237      30
 
BsaWI w'CCGG_w

Cuts at:    300    3818    4339    5017    5291    5438    5849     300 
   Size:       3518     521     678     274     147     411    1080

  Fragments arranged by size: 

                3518    1080     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1243    1273    2488    2518    1243 
   Size:         30    1215      30    5354

  Fragments arranged by size: 

                5354    1215      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    903     934    1264    1758    2371     903 
   Size:         31     330     494     613    5161

  Fragments arranged by size: 

                5161     613     494     330      31
 
BseYI C'CCAG_C

Cuts at:    148     371     877     924    1310    3555    4054    4812    5389 
   Size:        223     506      47     386    2245     499     758     577

Cuts at:   5389    5885     148 
   Size:        496     892

  Fragments arranged by size: 

                2245     892     758     577     506     499     496     386
                 223      47
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    589    1104    1608    1738    1818     589 
   Size:        515     504     130      80    5400

  Fragments arranged by size: 

                5400     515     504     130      80
 
BsiEI CG_ry'CG

Cuts at:    104    4171    4217    5425     104 
   Size:       4067      46    1208    1308

  Fragments arranged by size: 

                4067    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    653     947    1028    1288    2456    2633    3338    3643    4421 
   Size:        294      81     260    1168     177     705     305     778

Cuts at:   4421    4611    5403     653 
   Size:        190     792    1879

  Fragments arranged by size: 

                1879    1168     792     778     705     305     294     260
                 190     177      81
 
BsmI GAATG_Cn'

Cuts at:   1219    3616    1219 
   Size:       2397    4232
 
BsmBI CGTCTCn'nnnn_

Cuts at:    459     826    1498    2768     459 
   Size:        367     672    1270    4320

  Fragments arranged by size: 

                4320    1270     672     367
 
Bsp1286I G_dGCh'C

Cuts at:    251     403     424     641     653     877     947     956    1028 
   Size:        152      21     217      12     224      70       9      72

Cuts at:   1028    1288    1361    1514    1603    1794    1893    2051    2174 
   Size:        260      73     153      89     191      99     158     123

Cuts at:   2174    2456    2633    3338    3643    4254    4347    4421    4611 
   Size:        282     177     705     305     611      93      74     190

Cuts at:   4611    4673    5403     251 
   Size:         62     730    1477

  Fragments arranged by size: 

                1477     730     705     611     305     282     260     224
                 217     191     190     177     158     153     152     123
                  99      93      89      74      73      72      70      62
                  21      12       9
 
BspEI T'CCGG_A

Cuts at:   5849    5849 
   Size:       6629
 
BspHI T'CATG_A

Cuts at:   5805    5805 
   Size:       6629
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    561     662    1141    1177    1192    1357    3228    3369    3679 
   Size:        101     479      36      15     165    1871     141     310

Cuts at:   3679    3893    4195    4576     561 
   Size:        214     302     381    2614

  Fragments arranged by size: 

                2614    1871     479     381     310     302     214     165
                 141     101      36      15
 
BsrBI CCG'CTC

Cuts at:     50    4921    4975    6459    6624      50 
   Size:       4871      54    1484     165      55

  Fragments arranged by size: 

                4871    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1863    4541    1863 
   Size:       2678    3951
 
BsrFI r'CCGG_y

Cuts at:    264     300    1079    1322    1639    2843    3181    4627    4808 
   Size:         36     779     243     317    1204     338    1446     181

Cuts at:   4808    5017     264 
   Size:        209    1876

  Fragments arranged by size: 

                1876    1446    1204     779     338     317     243     209
                 181      36
 
BsrGI T'GTAC_A

Cuts at:   2132    2132 
   Size:       6629
 
BssSI C'ACGA_G

Cuts at:    912     964    4900    5258     912 
   Size:         52    3936     358    2283

  Fragments arranged by size: 

                3936    2283     358      52
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1349    1532    4006    1349 
   Size:        183    2474    3972

  Fragments arranged by size: 

                3972    2474     183
 
BstBI TT'CG_AA

Cuts at:   4990    4990 
   Size:       6629
 
BstEII G'GTnAC_C

Cuts at:    163    1436    3196     163 
   Size:       1273    1760    3596

  Fragments arranged by size: 

                3596    1760    1273
 
BstYI r'GATC_y

Cuts at:      1     480    1646    3009    3654    4121    4144    4479    4725 
   Size:        479    1166    1363     645     467      23     335     246

Cuts at:   4725    5737    5823    5835    6181       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1363    1166    1012     645     479     467     449     346
                 335     246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   3340    3340 
   Size:       6629
 
BtgI C'CryG_G

Cuts at:    107     243     467    1264    1612    1754    2507    3320     107 
   Size:        136     224     797     348     142     753     813    3416

  Fragments arranged by size: 

                3416     813     797     753     348     224     142     136
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2333    3084    4579    4760    4923    2333 
   Size:        751    1495     181     163    4039

  Fragments arranged by size: 

                4039    1495     751     181     163
 
BtsI GCAGTG_nn'

Cuts at:    556    1959    2247    3872     556 
   Size:       1403     288    1625    3313

  Fragments arranged by size: 

                3313    1625    1403     288
 
ClaI AT'CG_AT

Cuts at:   1586    1586 
   Size:       6629
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1524    1559    3012    3047    1524 
   Size:         35    1453      35    5106

  Fragments arranged by size: 

                5106    1453      35      35
 
DraI TTT'AAA

Cuts at:   1146    3628    1146 
   Size:       2482    4147
 
DraIII CAC_nnn'GTG

Cuts at:    741    1127    1239     741 
   Size:        386     112    6131

  Fragments arranged by size: 

                6131     386     112
 
DrdI GACnn_nn'nnGTC

Cuts at:   4335    5193    4335 
   Size:        858    5771
 
EaeI y'GGCC_r

Cuts at:    683    1077    1545    1590    2709    2778    4214    4388    4779 
   Size:        394     468      45    1119      69    1436     174     391

Cuts at:   4779    4806    6151     683 
   Size:         27    1345    1161

  Fragments arranged by size: 

                1436    1345    1161    1119     468     394     391     174
                  69      45      27
 
EagI C'GGCC_G

Cuts at:   4214    4214 
   Size:       6629
 
EarI CTCTTCn'nnn_

Cuts at:   2389    4652    4862    2389 
   Size:       2263     210    4156

  Fragments arranged by size: 

                4156    2263     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    676    4837    5147    5293    6439     676 
   Size:       4161     310     146    1146     866

  Fragments arranged by size: 

                4161    1146     866     310     146
 
EcoICRI GAG'CTC

Cuts at:    945    1026    3641     945 
   Size:         81    2615    3933

  Fragments arranged by size: 

                3933    2615      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    135     436     544     696     709    1446    1617    2103    2967 
   Size:        301     108     152      13     737     171     486     864

Cuts at:   2967    3120    4454    4886    5633    5901     135 
   Size:        153    1334     432     747     268     863

  Fragments arranged by size: 

                1334     864     863     747     737     486     432     301
                 268     171     153     152     108      13
 
EcoO109I rG'GnC_Cy

Cuts at:    420     421     874    1307    1357    1358    1817    1875    3520 
   Size:          1     453     433      50       1     459      58    1645

Cuts at:   3520    3768     420 
   Size:        248    3281

  Fragments arranged by size: 

                3281    1645     459     453     433     248      58      50
                   1       1
 
EcoRI G'AATT_C

Cuts at:   3633    3633 
   Size:       6629
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    372    4648     372 
   Size:       4276    2353
 
FspI TGC'GCA

Cuts at:   4410    4410 
   Size:       6629
 
HaeII r_GCGC'y

Cuts at:    233    1574    4063    4311    5333    6312     233 
   Size:       1341    2489     248    1022     979     550

  Fragments arranged by size: 

                2489    1341    1022     979     550     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    376     408     791     823    1243    1275    2488    2520    3109 
   Size:         32     383      32     420      32    1213      32     589

Cuts at:   3109    3141    4471    4501    4503    4533     376 
   Size:         32    1330      30       2      30    2472

  Fragments arranged by size: 

                2472    1330    1213     589     420     383      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1941    3668    1941 
   Size:       1727    4902
 
KasI G'GCGC_C

Cuts at:    229    1570    4059    4307     229 
   Size:       1341    2489     248    2551

  Fragments arranged by size: 

                2551    2489    1341     248
 
KpnI G_GTAC'C

Cuts at:   3649    3649 
   Size:       6629
 
MluI A'CGCG_T

Cuts at:   6062    6062 
   Size:       6629
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    482    1796    2771    5300    5484     482 
   Size:       1314     975    2529     184    1627

  Fragments arranged by size: 

                2529    1627    1314     975     184
 
MscI TGG'CCA

Cuts at:    685    4390     685 
   Size:       3705    2924
 
MslI CAynn'nnrTG

Cuts at:   4618    4745    4618 
   Size:        127    6502
 
MspA1I CmG'CkG

Cuts at:    245     469    1118    1595    2432    3740    4054    4414    5427 
   Size:        224     649     477     837    1308     314     360    1013

Cuts at:   5427    5672     245 
   Size:        245    1202

  Fragments arranged by size: 

                1308    1202    1013     837     649     477     360     314
                 245     224
 
NaeI GCC'GGC

Cuts at:    266    2845    3183    4810     266 
   Size:       2579     338    1627    2085

  Fragments arranged by size: 

                2579    2085    1627     338
 
NarI GG'CG_CC

Cuts at:    230    1571    4060    4308     230 
   Size:       1341    2489     248    2551

  Fragments arranged by size: 

                2551    2489    1341     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       6629
 
NgoMIV G'CCGG_C

Cuts at:    264    2843    3181    4808     264 
   Size:       2579     338    1627    2085

  Fragments arranged by size: 

                2579    2085    1627     338
 
NspI r_CATG'y

Cuts at:    514     856    1059    3682    4182    5089     514 
   Size:        342     203    2623     500     907    2054

  Fragments arranged by size: 

                2623    2054     907     500     342     203
 
PciI A'CATG_T

Cuts at:   1055    5085    1055 
   Size:       4030    2599
 
PflMI CCAn_nnn'nTGG

Cuts at:    113    1239    1588    2284     113 
   Size:       1126     349     696    4458

  Fragments arranged by size: 

                4458    1126     696     349
 
PfoI T'CCnGG_A

Cuts at:    938    1649     938 
   Size:        711    5918
 
PmeI GTTT'AAAC

Cuts at:   3628    3628 
   Size:       6629
 
PmlI CAC'GTG

Cuts at:    600    1127     600 
   Size:        527    6102
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5768    5800    5768 
   Size:         32    6597
 
PpuMI rG'GwC_Cy

Cuts at:   1307    1817    1875    3520    1307 
   Size:        510      58    1645    4416

  Fragments arranged by size: 

                4416    1645     510      58
 
PshAI GACnn'nnGTC

Cuts at:   2549    2549 
   Size:       6629
 
PspOMI G'GGCC_C

Cuts at:    247     420     873    1357     247 
   Size:        173     453     484    5519

  Fragments arranged by size: 

                5519     484     453     173
 
PstI C_TGCA'G

Cuts at:    659    3676    4361     659 
   Size:       3017     685    2927

  Fragments arranged by size: 

                3017    2927     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6629
 
PvuII CAG'CTG

Cuts at:   1118    4054    4414    1118 
   Size:       2936     360    3333

  Fragments arranged by size: 

                3333    2936     360
 
RsrII CG'GwC_CG

Cuts at:   4824    4824 
   Size:       6629
 
SacI G_AGCT'C

Cuts at:    947    1028    3643     947 
   Size:         81    2615    3933

  Fragments arranged by size: 

                3933    2615      81
 
SacII CC_GC'GG

Cuts at:    246     470     246 
   Size:        224    6405
 
SalI G'TCGA_C

Cuts at:   3666    3666 
   Size:       6629
 
SanDI GG'GwC_CC

Cuts at:   1307    1307 
   Size:       6629
 
SapI GCTCTTCn'nnn_

Cuts at:   4652    4862    4652 
   Size:        210    6419
 
SbfI CC_TGCA'GG

Cuts at:    659    3676     659 
   Size:       3017    3612
 
ScaI AGT'ACT

Cuts at:   6146    6146 
   Size:       6629
 
SfcI C'TryA_G

Cuts at:     33     588     655    3104    3435    3672    4357    5350    5541 
   Size:        555      67    2449     331     237     685     993     191

Cuts at:   5541      33 
   Size:       1121

  Fragments arranged by size: 

                2449    1121     993     685     555     331     237     191
                  67
 
SfoI GGC'GCC

Cuts at:    231    1572    4061    4309     231 
   Size:       1341    2489     248    2551

  Fragments arranged by size: 

                2551    2489    1341     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6629
 
SgrAI Cr'CCGG_yG

Cuts at:    300     300 
   Size:       6629
 
SmaI CCC'GGG

Cuts at:   1663    1787    3651    1663 
   Size:        124    1864    4641

  Fragments arranged by size: 

                4641    1864     124
 
SmlI C'TyrA_G

Cuts at:    376     988    3301    3575    3601    3784    4699    5191    5453 
   Size:        612    2313     274      26     183     915     492     262

Cuts at:   5453     376 
   Size:       1552

  Fragments arranged by size: 

                2313    1552     915     612     492     274     262     183
                  26
 
SnaBI TAC'GTA

Cuts at:   3153    3153 
   Size:       6629
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6629
 
SphI G_CATG'C

Cuts at:   3682    4182    3682 
   Size:        500    6129
 
StuI AGG'CCT

Cuts at:   1702    1702 
   Size:       6629
 
StyI C'CwwG_G

Cuts at:    107    1441    3005    3763    4117     107 
   Size:       1334    1564     758     354    2619

  Fragments arranged by size: 

                2619    1564    1334     758     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    129    1095    1879    2371    2662    3040    4212    4345    4804 
   Size:        966     784     492     291     378    1172     133     459

Cuts at:   4804    5013     129 
   Size:        209    1745

  Fragments arranged by size: 

                1745    1172     966     784     492     459     378     291
                 209     133
 
TatI w'GTAC_w

Cuts at:   2132    6144    2132 
   Size:       4012    2617
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6146    6178    6146 
   Size:         32    6597
 
Tth111I GACn'n_nGTC

Cuts at:   2527    4426    2527 
   Size:       1899    4730
 
XbaI T'CTAG_A

Cuts at:   3660    3660 
   Size:       6629
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    867    2217    3541    4124     867 
   Size:       1350    1324     583    3372

  Fragments arranged by size: 

                3372    1350    1324     583
 
XmaI C'CCGG_G

Cuts at:   1661    1785    3649    1661 
   Size:        124    1864    4641

  Fragments arranged by size: 

                4641    1864     124
 
XmnI GAAnn'nnTTC

Cuts at:    764     764 
   Size:       6629
 
ZraI GAC'GTC

Cuts at:    531    1335     531 
   Size:        804    5825
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AleI     AscI    AvrII     BaeI    BfrBI     BmtI
    BsiWI   BssHII    BstXI  BstZ17I    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PsiI    PspXI     PsrI    SexAI     SfiI     SrfI
     SspI     SwaI     XhoI
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