Restriction map for pF1KB4505
 (Linear) (Six-base) MAPSORT of: KIBB4505.seq  Check: 9209  from: 1  to: 3516



KIBB4505.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     453     554    1069    1249    3120    3261    3516 
   Size:        453     101     515     180    1871     141     255

  Fragments arranged by size: 

                1871     515     453     255     180     141     101
 
AatII G_ACGT'C

Cuts at:      0     425    1229    3516 
   Size:        425     804    2287

  Fragments arranged by size: 

                2287     804     425
 
AccI GT'mk_AC

Cuts at:      0    2453    3516 
   Size:       2453    1063
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588     601    1995    2859    3012    3516 
   Size:        588      13    1394     864     153     504

  Fragments arranged by size: 

                1394     864     588     504     153      13
 
AflIII A'CryG_T

Cuts at:      0     947    1018    1186    3516 
   Size:        947      71     168    2330

  Fragments arranged by size: 

                2330     947     168      71
 
AgeI A'CCGG_T

Cuts at:      0     192    3516 
   Size:        192    3324
 
AhdI GACnn_n'nnGTC

Cuts at:      0     282    3516 
   Size:        282    3234
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2369    3516 
   Size:       2369    1147
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     958     990    3516 
   Size:        958      32    2526

  Fragments arranged by size: 

                2526     958      32
 
ApaI G_GGCC'C

Cuts at:      0     143     316     769    1253    3516 
   Size:        143     173     453     484    2263

  Fragments arranged by size: 

                2263     484     453     173     143
 
ApaLI G'TGCA_C

Cuts at:      0    2344    2521    3516 
   Size:       2344     177     995

  Fragments arranged by size: 

                2344     995     177
 
ApoI r'AATT_y

Cuts at:      0    1033    2172    3516 
   Size:       1033    1139    1344

  Fragments arranged by size: 

                1344    1139    1033
 
AvaI C'yCGr_G

Cuts at:      0     787     840    1553    1677    3516 
   Size:        787      53     713     124    1839

  Fragments arranged by size: 

                1839     787     713     124      53
 
BamHI G'GATC_C

Cuts at:      0    1538    2901    3516 
   Size:       1538    1363     615

  Fragments arranged by size: 

                1538    1363     615
 
BanI G'GyrC_C

Cuts at:      0     121     394    1462    1753    1818    3335    3516 
   Size:        121     273    1068     291      65    1517     181

  Fragments arranged by size: 

                1517    1068     291     273     181     121      65
 
BanII G_rGCy'C

Cuts at:      0     143     316     533     769     839     848     920    1253 
   Size:        143     173     217     236      70       9      72     333

Cuts at:   1253    1495    1686    1943    2066    3516 
   Size:        242     191     257     123    1450

  Fragments arranged by size: 

                1450     333     257     242     236     217     191     173
                 143     123      72      70       9
 
BbeI G_GCGC'C

Cuts at:      0     125    1466    3516 
   Size:        125    1341    2050

  Fragments arranged by size: 

                2050    1341     125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1331    2192    2415    3453    3516 
   Size:       1331     861     223    1038      63

  Fragments arranged by size: 

                1331    1038     861     223      63
 
BbvCI CC'TCA_GC

Cuts at:      0     211    3516 
   Size:        211    3305
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3052    3086    3516 
   Size:       3052      34     430

  Fragments arranged by size: 

                3052     430      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     683    1736    2045    2650    2951    3516 
   Size:        683    1053     309     605     301     565

  Fragments arranged by size: 

                1053     683     605     565     309     301
 
BlpI GC'TnA_GC

Cuts at:      0     868    3516 
   Size:        868    2648
 
Bme1580I G_kGCm'C

Cuts at:      0     143     295     316     769    1253    1406    1785    2348 
   Size:        143     152      21     453     484     153     379     563

Cuts at:   2348    2525    3516 
   Size:        177     991

  Fragments arranged by size: 

                 991     563     484     453     379     177     153     152
                 143      21
 
BmgBI CAC'GTC

Cuts at:      0     852    1187    2544    3516 
   Size:        852     335    1357     972

  Fragments arranged by size: 

                1357     972     852     335
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1167    1393    1680    2943    3156    3516 
   Size:       1167     226     287    1263     213     360

  Fragments arranged by size: 

                1263    1167     360     287     226     213
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     363    3516 
   Size:        363    3153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     328     436    1338    1509    3516 
   Size:         27     301     108     902     171    2007

  Fragments arranged by size: 

                2007     902     301     171     108      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     211    1571    3516 
   Size:        211    1360    1945

  Fragments arranged by size: 

                1945    1360     211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     865    3178    3452    3478    3516 
   Size:        253     612    2313     274      26      38

  Fragments arranged by size: 

                2313     612     274     253      38      26
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     325     414     469    1157    1438    3516 
   Size:        325      89      55     688     281    2078

  Fragments arranged by size: 

                2078     688     325     281      89      55
 
BsaAI yAC'GTr

Cuts at:      0     492     573     762    1019    2343    3045    3516 
   Size:        492      81     189     257    1324     702     471

  Fragments arranged by size: 

                1324     702     492     471     257     189      81
 
BsaHI Gr'CG_yC

Cuts at:      0     122     422    1226    1463    2390    2681    2711    3516 
   Size:        122     300     804     237     927     291      30     805

  Fragments arranged by size: 

                 927     805     804     300     291     237     122      30
            
 
BsaWI w'CCGG_w

Cuts at:      0     192    3516 
   Size:        192    3324
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1135    1165    2380    2410    3516 
   Size:       1135      30    1215      30    1106

  Fragments arranged by size: 

                1215    1135    1106      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     795     826    1156    1650    2263    3516 
   Size:        795      31     330     494     613    1253

  Fragments arranged by size: 

                1253     795     613     494     330      31
 
BseYI C'CCAG_C

Cuts at:      0      40     263     769     816    1202    3447    3516 
   Size:         40     223     506      47     386    2245      69

  Fragments arranged by size: 

                2245     506     386     223      69      47      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     996    1500    1630    1710    3516 
   Size:        481     515     504     130      80    1806

  Fragments arranged by size: 

                1806     515     504     481     130      80
 
BsiHKAI G_wGCw'C

Cuts at:      0     545     839     920    1180    2348    2525    3230    3516 
   Size:        545     294      81     260    1168     177     705     286

  Fragments arranged by size: 

                1168     705     545     294     286     260     177      81
            
 
BsmI GAATG_Cn'

Cuts at:      0    1111    3508    3516 
   Size:       1111    2397       8

  Fragments arranged by size: 

                2397    1111       8
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     351     718    1390    2660    3516 
   Size:        351     367     672    1270     856

  Fragments arranged by size: 

                1270     856     672     367     351
 
Bsp1286I G_dGCh'C

Cuts at:      0     143     295     316     533     545     769     839     848 
   Size:        143     152      21     217      12     224      70       9

Cuts at:    848     920    1180    1253    1406    1495    1686    1785    1943 
   Size:         72     260      73     153      89     191      99     158

Cuts at:   1943    2066    2348    2525    3230    3516 
   Size:        123     282     177     705     286

  Fragments arranged by size: 

                 705     286     282     260     224     217     191     177
                 158     153     152     143     123      99      89      73
                  72      70      21      12       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     453     554    1033    1069    1084    1249    3120    3261 
   Size:        453     101     479      36      15     165    1871     141

Cuts at:   3261    3516 
   Size:        255

  Fragments arranged by size: 

                1871     479     453     255     165     141     101      36
                  15
 
BsrDI GCAATG_nn'

Cuts at:      0    1755    3516 
   Size:       1755    1761
 
BsrFI r'CCGG_y

Cuts at:      0     156     192     971    1214    1531    2735    3073    3516 
   Size:        156      36     779     243     317    1204     338     443

  Fragments arranged by size: 

                1204     779     443     338     317     243     156      36
            
 
BsrGI T'GTAC_A

Cuts at:      0    2024    3516 
   Size:       2024    1492
 
BssSI C'ACGA_G

Cuts at:      0     804     856    3516 
   Size:        804      52    2660

  Fragments arranged by size: 

                2660     804      52
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1241    1424    3516 
   Size:       1241     183    2092

  Fragments arranged by size: 

                2092    1241     183
 
BstEII G'GTnAC_C

Cuts at:      0      55    1328    3088    3516 
   Size:         55    1273    1760     428

  Fragments arranged by size: 

                1760    1273     428      55
 
BstYI r'GATC_y

Cuts at:      0     372    1538    2901    3516 
   Size:        372    1166    1363     615

  Fragments arranged by size: 

                1363    1166     615     372
 
Bsu36I CC'TnA_GG

Cuts at:      0    3232    3516 
   Size:       3232     284
 
BtgI C'CryG_G

Cuts at:      0     135     359    1156    1504    1646    2399    3212    3516 
   Size:        135     224     797     348     142     753     813     304

  Fragments arranged by size: 

                 813     797     753     348     304     224     142     135
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2225    2976    3516 
   Size:       2225     751     540

  Fragments arranged by size: 

                2225     751     540
 
BtsI GCAGTG_nn'

Cuts at:      0     448    1851    2139    3516 
   Size:        448    1403     288    1377

  Fragments arranged by size: 

                1403    1377     448     288
 
ClaI AT'CG_AT

Cuts at:      0    1478    3516 
   Size:       1478    2038
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1416    1451    2904    2939    3516 
   Size:       1416      35    1453      35     577

  Fragments arranged by size: 

                1453    1416     577      35      35
 
DraI TTT'AAA

Cuts at:      0    1038    3516 
   Size:       1038    2478
 
DraIII CAC_nnn'GTG

Cuts at:      0     633    1019    1131    3516 
   Size:        633     386     112    2385

  Fragments arranged by size: 

                2385     633     386     112
 
EaeI y'GGCC_r

Cuts at:      0     575     969    1437    1482    2601    2670    3516 
   Size:        575     394     468      45    1119      69     846

  Fragments arranged by size: 

                1119     846     575     468     394      69      45
 
EarI CTCTTCn'nnn_

Cuts at:      0    2281    3516 
   Size:       2281    1235
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     568    3516 
   Size:        568    2948
 
EcoICRI GAG'CTC

Cuts at:      0     837     918    3516 
   Size:        837      81    2598

  Fragments arranged by size: 

                2598     837      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     328     436     588     601    1338    1509    1995 
   Size:         27     301     108     152      13     737     171     486

Cuts at:   1995    2859    3012    3516 
   Size:        864     153     504

  Fragments arranged by size: 

                 864     737     504     486     301     171     153     152
                 108      27      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     312     313     766    1199    1249    1250    1709    1767 
   Size:        312       1     453     433      50       1     459      58

Cuts at:   1767    3412    3516 
   Size:       1645     104

  Fragments arranged by size: 

                1645     459     453     433     312     104      58      50
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     264    3516 
   Size:        264    3252
 
HaeII r_GCGC'y

Cuts at:      0     125    1466    3516 
   Size:        125    1341    2050

  Fragments arranged by size: 

                2050    1341     125
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     683     715    1135    1167    2380    2412 
   Size:        268      32     383      32     420      32    1213      32

Cuts at:   2412    3001    3033    3516 
   Size:        589      32     483

  Fragments arranged by size: 

                1213     589     483     420     383     268      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1833    3516 
   Size:       1833    1683
 
KasI G'GCGC_C

Cuts at:      0     121    1462    3516 
   Size:        121    1341    2054

  Fragments arranged by size: 

                2054    1341     121
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374    1688    2663    3516 
   Size:        374    1314     975     853

  Fragments arranged by size: 

                1314     975     853     374
 
MscI TGG'CCA

Cuts at:      0     577    3516 
   Size:        577    2939
 
MspA1I CmG'CkG

Cuts at:      0     137     361    1010    1487    2324    3516 
   Size:        137     224     649     477     837    1192

  Fragments arranged by size: 

                1192     837     649     477     224     137
 
NaeI GCC'GGC

Cuts at:      0     158    2737    3075    3516 
   Size:        158    2579     338     441

  Fragments arranged by size: 

                2579     441     338     158
 
NarI GG'CG_CC

Cuts at:      0     122    1463    3516 
   Size:        122    1341    2053

  Fragments arranged by size: 

                2053    1341     122
 
NgoMIV G'CCGG_C

Cuts at:      0     156    2735    3073    3516 
   Size:        156    2579     338     443

  Fragments arranged by size: 

                2579     443     338     156
 
NspI r_CATG'y

Cuts at:      0     406     748     951    3516 
   Size:        406     342     203    2565

  Fragments arranged by size: 

                2565     406     342     203
 
PciI A'CATG_T

Cuts at:      0     947    3516 
   Size:        947    2569
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1131    1480    2176    3516 
   Size:       1131     349     696    1340

  Fragments arranged by size: 

                1340    1131     696     349
 
PfoI T'CCnGG_A

Cuts at:      0     830    1541    3516 
   Size:        830     711    1975

  Fragments arranged by size: 

                1975     830     711
 
PmlI CAC'GTG

Cuts at:      0     492    1019    3516 
   Size:        492     527    2497

  Fragments arranged by size: 

                2497     527     492
 
PpuMI rG'GwC_Cy

Cuts at:      0    1199    1709    1767    3412    3516 
   Size:       1199     510      58    1645     104

  Fragments arranged by size: 

                1645    1199     510     104      58
 
PshAI GACnn'nnGTC

Cuts at:      0    2441    3516 
   Size:       2441    1075
 
PspOMI G'GGCC_C

Cuts at:      0     139     312     765    1249    3516 
   Size:        139     173     453     484    2267

  Fragments arranged by size: 

                2267     484     453     173     139
 
PstI C_TGCA'G

Cuts at:      0     551    3516 
   Size:        551    2965
 
PvuII CAG'CTG

Cuts at:      0    1010    3516 
   Size:       1010    2506
 
SacI G_AGCT'C

Cuts at:      0     839     920    3516 
   Size:        839      81    2596

  Fragments arranged by size: 

                2596     839      81
 
SacII CC_GC'GG

Cuts at:      0     138     362    3516 
   Size:        138     224    3154

  Fragments arranged by size: 

                3154     224     138
 
SanDI GG'GwC_CC

Cuts at:      0    1199    3516 
   Size:       1199    2317
 
SbfI CC_TGCA'GG

Cuts at:      0     551    3516 
   Size:        551    2965
 
SfcI C'TryA_G

Cuts at:      0     480     547    2996    3327    3516 
   Size:        480      67    2449     331     189

  Fragments arranged by size: 

                2449     480     331     189      67
 
SfoI GGC'GCC

Cuts at:      0     123    1464    3516 
   Size:        123    1341    2052

  Fragments arranged by size: 

                2052    1341     123
 
SgrAI Cr'CCGG_yG

Cuts at:      0     192    3516 
   Size:        192    3324
 
SmaI CCC'GGG

Cuts at:      0    1555    1679    3516 
   Size:       1555     124    1837

  Fragments arranged by size: 

                1837    1555     124
 
SmlI C'TyrA_G

Cuts at:      0     268     880    3193    3467    3493    3516 
   Size:        268     612    2313     274      26      23

  Fragments arranged by size: 

                2313     612     274     268      26      23
 
SnaBI TAC'GTA

Cuts at:      0    3045    3516 
   Size:       3045     471
 
StuI AGG'CCT

Cuts at:      0    1594    3516 
   Size:       1594    1922
 
StyI C'CwwG_G

Cuts at:      0    1333    2897    3516 
   Size:       1333    1564     619

  Fragments arranged by size: 

                1564    1333     619
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21     987    1771    2263    2554    2932    3516 
   Size:         21     966     784     492     291     378     584

  Fragments arranged by size: 

                 966     784     584     492     378     291      21
 
TatI w'GTAC_w

Cuts at:      0    2024    3516 
   Size:       2024    1492
 
Tth111I GACn'n_nGTC

Cuts at:      0    2419    3516 
   Size:       2419    1097
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     759    2109    3433    3516 
   Size:        759    1350    1324      83

  Fragments arranged by size: 

                1350    1324     759      83
 
XmaI C'CCGG_G

Cuts at:      0    1553    1677    3516 
   Size:       1553     124    1839

  Fragments arranged by size: 

                1839    1553     124
 
XmnI GAAnn'nnTTC

Cuts at:      0     656    3516 
   Size:        656    2860
 
ZraI GAC'GTC

Cuts at:      0     423    1227    3516 
   Size:        423     804    2289

  Fragments arranged by size: 

                2289     804     423
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII     KasI     MmeI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SacII    SanDI     SbfI
     SfcI     SfoI    SgrAI     SmaI     SmlI    SnaBI     StuI     StyI
    TaqII     TatI  Tth111I     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AleI    AlwNI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI    BglII     BmtI
    BsaBI    BsiEI    BsiWI    BspEI    BspHI    BsrBI   BssHII    BstBI
    BstXI  BstZ17I     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PpiI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI
     SapI     ScaI    SexAI     SfiI     SgfI     SpeI     SphI     SrfI
     SspI     SwaI     TstI     XbaI     XhoI
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