Restriction map for pF1KB4158
 (Linear) (Six-base) MAPSORT of: KIBB4158.seq  Check: 1471  from: 1  to: 1476



KIBB4158.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0     348     863    1476 
   Size:        348     515     613

  Fragments arranged by size: 

                 613     515     348
 
AclI AA'CG_TT

Cuts at:      0     822    1476 
   Size:        822     654
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     749     820    1208    1476 
   Size:        430     319      71     388     268

  Fragments arranged by size: 

                 430     388     319     268      71
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1384    1476 
   Size:       1384      92
 
ApoI r'AATT_y

Cuts at:      0      98     420     877     936    1476 
   Size:         98     322     457      59     540

  Fragments arranged by size: 

                 540     457     322      98      59
 
AvaI C'yCGr_G

Cuts at:      0    1449    1476 
   Size:       1449      27
 
BanI G'GyrC_C

Cuts at:      0     904    1406    1476 
   Size:        904     502      70

  Fragments arranged by size: 

                 904     502      70
 
BanII G_rGCy'C

Cuts at:      0     989    1476 
   Size:        989     487
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     305     656    1476 
   Size:        305     351     820

  Fragments arranged by size: 

                 820     351     305
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     641    1476 
   Size:        641     835
 
BglI GCCn_nnn'nGGC

Cuts at:      0     793    1476 
   Size:        793     683
 
BlpI GC'TnA_GC

Cuts at:      0     477    1476 
   Size:        477     999
 
BmtI G_CTAG'C

Cuts at:      0     437    1476 
   Size:        437    1039
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     530     704    1476 
   Size:        302     228     174     772

  Fragments arranged by size: 

                 772     302     228     174
 
BsaAI yAC'GTr

Cuts at:      0     559    1476 
   Size:        559     917
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     440     470     708     738    1476 
   Size:        440      30     238      30     738

  Fragments arranged by size: 

                 738     440     238      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67    1476 
   Size:         67    1409
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3    1476 
   Size:          3    1473
 
BsmI GAATG_Cn'

Cuts at:      0      70    1373    1476 
   Size:         70    1303     103

  Fragments arranged by size: 

                1303     103      70
 
Bsp1286I G_dGCh'C

Cuts at:      0     989    1476 
   Size:        989     487
 
BspHI T'CATG_A

Cuts at:      0     228    1476 
   Size:        228    1248
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      25    1476 
   Size:         25    1451
 
BsrGI T'GTAC_A

Cuts at:      0    1296    1476 
   Size:       1296     180
 
BssSI C'ACGA_G

Cuts at:      0    1397    1476 
   Size:       1397      79
 
BstEII G'GTnAC_C

Cuts at:      0    1454    1476 
   Size:       1454      22
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     641    1476 
   Size:        641     835
 
BstYI r'GATC_y

Cuts at:      0     564     812    1476 
   Size:        564     248     664

  Fragments arranged by size: 

                 664     564     248
 
BstZ17I GTA'TAC

Cuts at:      0     349    1476 
   Size:        349    1127
 
BtgI C'CryG_G

Cuts at:      0     808    1476 
   Size:        808     668
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1429    1476 
   Size:       1429      47
 
BtsI GCAGTG_nn'

Cuts at:      0    1368    1476 
   Size:       1368     108
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1383    1476 
   Size:       1383      93
 
EarI CTCTTCn'nnn_

Cuts at:      0     239    1385    1476 
   Size:        239    1146      91

  Fragments arranged by size: 

                1146     239      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     430     530     704     749     820    1208    1476 
   Size:        302     128     100     174      45      71     388     268

  Fragments arranged by size: 

                 388     302     268     174     128     100      71      45
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21    1476 
   Size:         21    1455
 
EcoO109I rG'GnC_Cy

Cuts at:      0     405    1476 
   Size:        405    1071
 
EcoRV GAT'ATC

Cuts at:      0     538    1476 
   Size:        538     938
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255     685     717    1476 
   Size:        223      32     430      32     759

  Fragments arranged by size: 

                 759     430     223      32      32
 
HincII GTy'rAC

Cuts at:      0     820    1031    1476 
   Size:        820     211     445

  Fragments arranged by size: 

                 820     445     211
 
HindIII A'AGCT_T

Cuts at:      0     145    1190    1476 
   Size:        145    1045     286

  Fragments arranged by size: 

                1045     286     145
 
MfeI C'AATT_G

Cuts at:      0    1052    1476 
   Size:       1052     424
 
MslI CAynn'nnrTG

Cuts at:      0     386     708    1476 
   Size:        386     322     768

  Fragments arranged by size: 

                 768     386     322
 
MspA1I CmG'CkG

Cuts at:      0     454    1476 
   Size:        454    1022
 
NcoI C'CATG_G

Cuts at:      0     808    1476 
   Size:        808     668
 
NheI G'CTAG_C

Cuts at:      0     433    1476 
   Size:        433    1043
 
NspI r_CATG'y

Cuts at:      0     599    1476 
   Size:        599     877
 
PpuMI rG'GwC_Cy

Cuts at:      0     405    1476 
   Size:        405    1071
 
PvuII CAG'CTG

Cuts at:      0     454    1476 
   Size:        454    1022
 
SexAI A'CCwGG_T

Cuts at:      0      22    1476 
   Size:         22    1454
 
SfcI C'TryA_G

Cuts at:      0     321     860    1476 
   Size:        321     539     616

  Fragments arranged by size: 

                 616     539     321
 
SnaBI TAC'GTA

Cuts at:      0     559    1476 
   Size:        559     917
 
SspI AAT'ATT

Cuts at:      0     216    1476 
   Size:        216    1260
 
StuI AGG'CCT

Cuts at:      0    1169    1476 
   Size:       1169     307
 
StyI C'CwwG_G

Cuts at:      0     443     808    1476 
   Size:        443     365     668

  Fragments arranged by size: 

                 668     443     365
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     821    1476 
   Size:        821     655
 
TatI w'GTAC_w

Cuts at:      0    1296    1349    1476 
   Size:       1296      53     127

  Fragments arranged by size: 

                1296     127      53
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     440     472     566     598     708     740    1408    1440 
   Size:        440      32      94      32     110      32     668      32

Cuts at:   1440    1476 
   Size:         36

  Fragments arranged by size: 

                 668     440     110      94      36      32      32      32
                  32
 
XbaI T'CTAG_A

Cuts at:      0     360    1476 
   Size:        360    1116
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     587     707    1012    1476 
   Size:        587     120     305     464

  Fragments arranged by size: 

                 587     464     305     120
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AlfI     ApoI     AvaI     BanI    BanII
     BbsI    BciVI     BglI     BlpI     BmtI     BpmI    BsaAI    BsaXI
    BseRI     BsgI     BsmI Bsp1286I    BspHI    BspMI    BsrGI    BssSI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DrdI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRV    Hin4I   HincII  HindIII
     MfeI     MslI   MspA1I     NcoI     NheI     NspI    PpuMI    PvuII
    SexAI     SfcI    SnaBI     SspI     StuI     StyI    TaqII     TatI
     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI
    BglII Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI   Bsu36I
     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI  EcoICRI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     SwaI  Tth111I     XhoI     XmaI     XmnI
     ZraI
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