Restriction map for pF1KB4066
 (Linear) (Six-base) MAPSORT of: KIBB4066.seq  Check: 2855  from: 1  to: 1272



KIBB4066.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

Acc65I G'GTAC_C

Cuts at:      0    1120    1272 
   Size:       1120     152
 
AclI AA'CG_TT

Cuts at:      0      44    1272 
   Size:         44    1228
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229    1272 
   Size:        229    1043
 
AflII C'TTAA_G

Cuts at:      0     948    1272 
   Size:        948     324
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162    1272 
   Size:        162    1110
 
ApoI r'AATT_y

Cuts at:      0     690     733     988    1073    1272 
   Size:        690      43     255      85     199

  Fragments arranged by size: 

                 690     255     199      85      43
 
AseI AT'TA_AT

Cuts at:      0    1163    1272 
   Size:       1163     109
 
BamHI G'GATC_C

Cuts at:      0     657    1272 
   Size:        657     615
 
BanI G'GyrC_C

Cuts at:      0    1120    1272 
   Size:       1120     152
 
BanII G_rGCy'C

Cuts at:      0     137     193    1272 
   Size:        137      56    1079

  Fragments arranged by size: 

                1079     137      56
 
BglI GCCn_nnn'nGGC

Cuts at:      0     641    1272 
   Size:        641     631
 
BglII A'GATC_T

Cuts at:      0     900    1272 
   Size:        900     372
 
BlpI GC'TnA_GC

Cuts at:      0     754    1272 
   Size:        754     518
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1256    1272 
   Size:       1256      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     142     874    1272 
   Size:          1     141     732     398

  Fragments arranged by size: 

                 732     398     141       1
 
Bpu10I CC'TnA_GC

Cuts at:      0     254     344    1272 
   Size:        254      90     928

  Fragments arranged by size: 

                 928     254      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455    1272 
   Size:        455     817
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     117     363     841    1272 
   Size:        117     246     478     431

  Fragments arranged by size: 

                 478     431     246     117
 
BsaAI yAC'GTr

Cuts at:      0      62    1272 
   Size:         62    1210
 
BsaWI w'CCGG_w

Cuts at:      0     300    1272 
   Size:        300     972
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145    1272 
   Size:        145    1127
 
BseYI C'CCAG_C

Cuts at:      0     857    1272 
   Size:        857     415
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424    1272 
   Size:        424     848
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     193    1272 
   Size:        137      56    1079

  Fragments arranged by size: 

                1079     137      56
 
BspEI T'CCGG_A

Cuts at:      0     300    1272 
   Size:        300     972
 
BsrDI GCAATG_nn'

Cuts at:      0    1184    1272 
   Size:       1184      88
 
BsrGI T'GTAC_A

Cuts at:      0     546     819    1272 
   Size:        546     273     453

  Fragments arranged by size: 

                 546     453     273
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     750    1238    1272 
   Size:        750     488      34

  Fragments arranged by size: 

                 750     488      34
 
BstYI r'GATC_y

Cuts at:      0     657     900    1272 
   Size:        657     243     372

  Fragments arranged by size: 

                 657     372     243
 
BtgI C'CryG_G

Cuts at:      0     196     594    1272 
   Size:        196     398     678

  Fragments arranged by size: 

                 678     398     196
 
BtsI GCAGTG_nn'

Cuts at:      0    1249    1272 
   Size:       1249      23
 
EarI CTCTTCn'nnn_

Cuts at:      0     205    1272 
   Size:        205    1067
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     105    1272 
   Size:        105    1167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     142     229     874    1272 
   Size:          1     141      87     645     398

  Fragments arranged by size: 

                 645     398     141      87       1
 
FspI TGC'GCA

Cuts at:      0     836    1272 
   Size:        836     436
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     366     398    1272 
   Size:        366      32     874

  Fragments arranged by size: 

                 874     366      32
 
KpnI G_GTAC'C

Cuts at:      0    1124    1272 
   Size:       1124     148
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1035    1272 
   Size:       1035     237
 
MslI CAynn'nnrTG

Cuts at:      0     446    1272 
   Size:        446     826
 
MspA1I CmG'CkG

Cuts at:      0     491     761     861    1272 
   Size:        491     270     100     411

  Fragments arranged by size: 

                 491     411     270     100
 
NspI r_CATG'y

Cuts at:      0     820     841    1272 
   Size:        820      21     431

  Fragments arranged by size: 

                 820     431      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     519    1201    1272 
   Size:        519     682      71

  Fragments arranged by size: 

                 682     519      71
 
PfoI T'CCnGG_A

Cuts at:      0      17    1272 
   Size:         17    1255
 
PmlI CAC'GTG

Cuts at:      0      62    1272 
   Size:         62    1210
 
PstI C_TGCA'G

Cuts at:      0     746    1272 
   Size:        746     526
 
PvuII CAG'CTG

Cuts at:      0     761     861    1272 
   Size:        761     100     411

  Fragments arranged by size: 

                 761     411     100
 
SapI GCTCTTCn'nnn_

Cuts at:      0     205    1272 
   Size:        205    1067
 
SfcI C'TryA_G

Cuts at:      0     742     876    1156    1272 
   Size:        742     134     280     116

  Fragments arranged by size: 

                 742     280     134     116
 
SmlI C'TyrA_G

Cuts at:      0     470     948    1272 
   Size:        470     478     324

  Fragments arranged by size: 

                 478     470     324
 
StuI AGG'CCT

Cuts at:      0     252     644    1272 
   Size:        252     392     628

  Fragments arranged by size: 

                 628     392     252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     704     871    1272 
   Size:        704     167     401

  Fragments arranged by size: 

                 704     401     167
 
TatI w'GTAC_w

Cuts at:      0     546     819    1272 
   Size:        546     273     453

  Fragments arranged by size: 

                 546     453     273
 
XbaI T'CTAG_A

Cuts at:      0     942    1272 
   Size:        942     330
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     284    1272 
   Size:        284     988
 
XmnI GAAnn'nnTTC

Cuts at:      0     324    1145    1272 
   Size:        324     821     127

  Fragments arranged by size: 

                 821     324     127
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI    AflII    AlwNI     ApoI     AseI    BamHI
     BanI    BanII     BglI    BglII     BlpI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BseRI    BseYI     BsgI Bsp1286I
    BspEI    BsrDI    BsrGI   BstAPI    BstYI     BtgI     BtsI     EarI
     EciI  Eco57MI     FspI    Hin4I     KpnI     MmeI     MslI   MspA1I
     NspI    PflMI     PfoI     PmlI     PstI    PvuII     SapI     SfcI
     SmlI     StuI    TaqII     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
 Bme1580I    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TstI  Tth111I     XhoI     XmaI     ZraI
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