Restriction map for pF1KB3962
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       EcoT22I     FauI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Van91I      XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          426  GTCTAC               GT^[AC][GT]AC
AflIII         15  ACATGT               A^C[GA][CT]GT
AocI         1505  CCTCAGG              CC^T[ACGT]AGG
Asp700I       279  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI           96  TGGCCA               TGG^CCA
BclI          198  TGATCA               T^GATCA
BsaAI         173  CACGTA               [CT]AC^GT[GA]
BseRI         370  GAGGAG               GAGGAG(10/8)
BsgI          467  CTGCAC               GTGCAG(16/14)
BsiEI         931  CGACCG               CG[GA][CT]^CG
BspLU11I       15  ACATGT               A^CATGT
BstAPI        460  GCACAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1047  AGATCC               [GA]^GATC[CT]
Bsu36I       1505  CCTCAGG              CC^T[ACGT]AGG
CfoI           85  GCGC                 GCG^C
DsaI          758  CCATGG               C^C[GA][CT]GG
EaeI           96  TGGCCA               [CT]^GGCC[GA]
Eco81I       1505  CCTCAGG              CC^T[ACGT]AGG
FbaI          198  TGATCA               T^GATCA
HapII         976  CCGG                 C^CGG
HhaI           85  GCGC                 GCG^C
HinP1I         85  GCGC                 G^CGC
HindIII       293  AAGCTT               A^AGCTT
HpaII         976  CCGG                 C^CGG
MaeII         174  ACGT                 A^CGT
MfeI          620  CAATTG               C^AATTG
MflI         1047  AGATCC               [GA]^GATC[CT]
MluNI          96  TGGCCA               TGG^CCA
MscI           96  TGGCCA               TGG^CCA
MspI          976  CCGG                 C^CGG
MunI          620  CAATTG               C^AATTG
NcoI          758  CCATGG               C^CATGG
PshAI         932  GACCGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI         1031  GAAGAGC              GCTCTTC(1/4)
ScaI          583  AGTACT               AGT^ACT
XbaI          324  TCTAGA               T^CTAGA
XcmI         1008  CCAAAGTACAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1047  AGATCC               [GA]^GATC[CT]
XmnI          279  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI         1048  GATCC                GGATC(4/5)
              117  GGATC               
AlwNI        1406  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1454  CAGAGCCTG           
AspI          181  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              912  GACTCAGTC           
BanI          600  GGCACC               G^G[CT][GA]CC
              762  GGTGCC              
BbsI         1153  GAAGAC               GAAGAC(2/6)
             1315  GAAGAC              
BfaI          325  CTAG                 C^TAG
              767  CTAG                
BpmI          860  CTCCAG               CTGGAG(16/14)
              983  CTCCAG              
BpuAI        1153  GAAGAC               GAAGAC(2/6)
             1315  GAAGAC              
BsmFI         625  GTCCC                GGGAC(10/14)
              736  GTCCC               
DraII          41  AGGACCT              [GA]G^G[ACGT]CC[CT]
              786  AGGTCCT             
EarI          647  CTCTTC               CTCTTC(1/4)
             1031  GAAGAG              
EcoNI         608  CCTCTAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              628  CCTGTACCAGG         
EcoO109I       41  AGGACCT              [GA]G^G[ACGT]CC[CT]
              786  AGGTCCT             
EcoRI          21  GAATTC               G^AATTC
              283  GAATTC              
EcoT14I       758  CCATGG               C^C[AT][AT]GG
             1400  CCAAGG              
Ksp632I       647  CTCTTC               CTCTTC(1/4)
             1031  GAAGAG              
MaeI          325  CTAG                 C^TAG
              767  CTAG                
NspI           15  ACATGT               [GA]CATG^[CT]
              720  GCATGT              
PflFI         181  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              912  GACTCAGTC           
PpuMI          41  AGGACCT              [GA]G^G[AT]CC[CT]
              786  AGGTCCT             
PstI          731  CTGCAG               CTGCA^G
             1180  CTGCAG              
StyI          758  CCATGG               C^C[AT][AT]GG
             1400  CCAAGG              
TaqI          747  TCGA                 T^CGA
              930  TCGA                
Tth111I       181  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              912  GACTCAGTC           
TthHB8I       747  TCGA                 T^CGA
              930  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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