Restriction map for pF1KB3956
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BamHI       BbeI        
BbvCI       BcgI        BciVI       BfaI        BfrI        
BlnI        BlpI        BpmI        Bpu1102I    BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoT22I     FseI        HaeII       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI         1055  GGCGTC               G[GA]^CG[CT]C
AlwNI         761  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          699  GGGCCC               GGGCC^C
AvaI          740  CCCGGG               C^[CT]CG[GA]G
BbrPI         373  CACGTG               CAC^GTG
BbsI          813  GAAGAC               GAAGAC(2/6)
BclI          980  TGATCA               T^GATCA
BpuAI         813  GAAGAC               GAAGAC(2/6)
BsaHI        1055  GGCGTC               G[GA]^CG[CT]C
BseRI          63  CTCCTC               GAGGAG(10/8)
BsgI         1048  CTGCAC               GTGCAG(16/14)
BsmI          836  GCATTC               GAATGC(1/-1)
BsoBI         740  CCCGGG               C^[CT]CG[GA]G
BspMI         398  ACCTGC               ACCTGC(4/8)
BstEII        708  GGTCACC              G^GT[ACGT]ACC
BstPI         708  GGTCACC              G^GT[ACGT]ACC
DdeI         1099  CTCAG                C^T[ACGT]AG
DraII         699  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        987  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          487  CCACGG               C^C[GA][CT]GG
EcoO109I      699  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       708  GGTCACC              G^GT[ACGT]ACC
EcoRV         458  GATATC               GAT^ATC
EcoT14I       524  CCTTGG               C^C[AT][AT]GG
FbaI          980  TGATCA               T^GATCA
HgaI         1056  GCGTC                GACGC(5/10)
Hin1I        1055  GGCGTC               G[GA]^CG[CT]C
HincII        391  GTCAAC               GT[CT]^[GA]AC
HindII        391  GTCAAC               GT[CT]^[GA]AC
HinfI         786  GATTC                G^A[ACGT]TC
MslI          960  CATCGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1066  CAGCGG               C[AC]G^C[GT]G
PmaCI         373  CACGTG               CAC^GTG
PmlI          373  CACGTG               CAC^GTG
SacI          745  GAGCTC               GAGCT^C
ScaI         1025  AGTACT               AGT^ACT
SmaI          740  CCCGGG               CCC^GGG
StyI          524  CCTTGG               C^C[AT][AT]GG
TfiI          786  GATTC                G^A[AT]TC
Tsp509I       496  AATT                 ^AATT
XmaCI         740  CCCGGG               C^CCGGG
XmaI          740  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          346  GTAC                 GT^AC
             1026  GTAC                
AlwI          642  GGATC                GGATC(4/5)
              734  GGATC               
Asp700I       612  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1023  GAAGTACTTC          
AvaII         140  GGACC                G^G[AT]CC
              819  GGTCC               
AviII         251  TGCGCA               TGC^GCA
              401  TGCGCA              
BanI          864  GGCACC               G^G[CT][GA]CC
             1113  GGTGCC              
BanII         699  GGGCCC               G[GA]GC[CT]^C
              745  GAGCTC              
BglI          277  GCCAACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              433  GCCTTGCTGGC         
BglII         614  AGATCT               A^GATCT
              629  AGATCT              
BsmAI         409  GTCTC                GTCTC(1/5)
              421  GTCTC               
BsmBI         408  CGTCTC               CGTCTC(1/5)
              420  CGTCTC              
BsmFI         763  GTCCC                GGGAC(10/14)
             1058  GTCCC               
EarI          673  CTCTTC               CTCTTC(1/4)
             1126  GAAGAG              
FauI          236  CCCGC                CCCGC(4/6)
             1068  GCGGG               
FspI          251  TGCGCA               TGC^GCA
              401  TGCGCA              
HapII         138  CCGG                 C^CGG
              741  CCGG                
HpaII         138  CCGG                 C^CGG
              741  CCGG                
Ksp632I       673  CTCTTC               CTCTTC(1/4)
             1126  GAAGAG              
MseI           41  TTAA                 T^TAA
               77  TTAA                
MspI          138  CCGG                 C^CGG
              741  CCGG                
RsaI          346  GTAC                 GT^AC
             1026  GTAC                
Tru9I          41  TTAA                 T^TAA
               77  TTAA                
XcmI          428  CCAATGCCTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1121  CCACAGAAGAGGTGG     
XmnI          612  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1023  GAAGTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AspHI       BalI        BbvI        
BcnI        BmyI        BsaAI       BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HhaI        HinP1I      
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XhoII       
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