Restriction map for pF1KB3715
 (Circular) (Six-base) MAPSORT of: pF1KB3715.seq  Check: 4066  from: 1  to: 3737



pF1KB3715  3737 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AatII G_ACGT'C

Cuts at:    190     440     190 
   Size:        250    3487
 
AccI GT'mk_AC

Cuts at:    775     775 
   Size:       3737
 
Acc65I G'GTAC_C

Cuts at:    753     753 
   Size:       3737
 
AclI AA'CG_TT

Cuts at:   3577    3577 
   Size:       3737
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1562    1994    2741    3009    1562 
   Size:        432     747     268    2290

  Fragments arranged by size: 

                2290     747     432     268
 
AflIII A'CryG_T

Cuts at:    612    1089    2193    3170     612 
   Size:        477    1104     977    1179

  Fragments arranged by size: 

                1179    1104     977     477
 
AgeI A'CCGG_T

Cuts at:   2125    2125 
   Size:       3737
 
AhdI GACnn_n'nnGTC

Cuts at:    258     258 
   Size:       3737
 
AlwNI CAG_nnn'CTG

Cuts at:   1042    2609    1042 
   Size:       1567    2170
 
ApaLI G'TGCA_C

Cuts at:   2507    2507 
   Size:       3737
 
ApoI r'AATT_y

Cuts at:    145     741    3090    3346     145 
   Size:        596    2349     256     536

  Fragments arranged by size: 

                2349     596     536     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3737
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3737
 
AvaI C'yCGr_G

Cuts at:    757     757 
   Size:       3737
 
BamHI G'GATC_C

Cuts at:    762     762 
   Size:       3737
 
BanI G'GyrC_C

Cuts at:    753    1167    1415    1450     753 
   Size:        414     248      35    3040

  Fragments arranged by size: 

                3040     414     248      35
 
BanII G_rGCy'C

Cuts at:    704     751    1781     704 
   Size:         47    1030    2660

  Fragments arranged by size: 

                2660    1030      47
 
BbeI G_GCGC'C

Cuts at:   1171    1419    1171 
   Size:        248    3489
 
BbsI GAAGACnn'nnnn_

Cuts at:    190     586     190 
   Size:        396    3341
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    589     623     589 
   Size:         34    3703
 
BciVI GTATCCnnnnn_n'

Cuts at:   1635    2396    3737    1635 
   Size:        761    1341    1635

  Fragments arranged by size: 

                1635    1341     761
 
BclI T'GATC_A

Cuts at:   1257    1257 
   Size:       3737
 
BglII A'GATC_T

Cuts at:   1252    3289    1252 
   Size:       2037    1700
 
BlpI GC'TnA_GC

Cuts at:    849     849 
   Size:       3737
 
Bme1580I G_kGCm'C

Cuts at:   1362    1455    2511    1362 
   Size:         93    1056    2588

  Fragments arranged by size: 

                2588    1056      93
 
BmgBI CAC'GTC

Cuts at:    613     613 
   Size:       3737
 
BmrI ACTGGGnnnn_n'

Cuts at:    949    1030    1365    3509     949 
   Size:         81     335    2144    1177

  Fragments arranged by size: 

                2144    1177     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2104    2104 
   Size:       3737
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    913    1792    2284    2582     913 
   Size:        879     492     298    2068

  Fragments arranged by size: 

                2068     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    562    3441     562 
   Size:       2879     858
 
BsaAI yAC'GTr

Cuts at:   1090    1090 
   Size:       3737
 
BsaBI GATnn'nnATC

Cuts at:      6     517     676    1251    3288       6 
   Size:        511     159     575    2037     455

  Fragments arranged by size: 

                2037     575     511     455     159
 
BsaHI Gr'CG_yC

Cuts at:    187     437     619    1168    1416    2118    3614     187 
   Size:        250     182     549     248     702    1496     310

  Fragments arranged by size: 

                1496     702     549     310     250     248     182
 
BsaWI w'CCGG_w

Cuts at:    926    1447    2125    2399    2546    2957     926 
   Size:        521     678     274     147     411    1706

  Fragments arranged by size: 

                1706     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    538     538 
   Size:       3737
 
BseYI C'CCAG_C

Cuts at:    346    1162    1920    2497    2993     346 
   Size:        816     758     577     496    1090

  Fragments arranged by size: 

                1090     816     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1279    1325    2533     104 
   Size:       1175      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1175      46
 
BsiHKAI G_wGCw'C

Cuts at:    751    1529    1719    2511     751 
   Size:        778     190     792    1977

  Fragments arranged by size: 

                1977     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    194     194 
   Size:       3737
 
Bsp1286I G_dGCh'C

Cuts at:    704     751    1362    1455    1529    1719    1781    2511     704 
   Size:         47     611      93      74     190      62     730    1930

  Fragments arranged by size: 

                1930     730     611     190      93      74      62      47
            
 
BspEI T'CCGG_A

Cuts at:   2957    2957 
   Size:       3737
 
BspHI T'CATG_A

Cuts at:   2913    2913 
   Size:       3737
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    787    1001    1303    1684     787 
   Size:        214     302     381    2840

  Fragments arranged by size: 

                2840     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2029    2083    3567    3732      50 
   Size:       1979      54    1484     165      55

  Fragments arranged by size: 

                1979    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1649    1649 
   Size:       3737
 
BsrFI r'CCGG_y

Cuts at:   1735    1916    2125    1735 
   Size:        181     209    3347

  Fragments arranged by size: 

                3347     209     181
 
BssHII G'CGCG_C

Cuts at:    537     537 
   Size:       3737
 
BssSI C'ACGA_G

Cuts at:   2008    2366    2008 
   Size:        358    3379
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1114    1114 
   Size:       3737
 
BstBI TT'CG_AA

Cuts at:   2098    2098 
   Size:       3737
 
BstXI CCAn_nnnn'nTGG

Cuts at:    276     276 
   Size:       3737
 
BstYI r'GATC_y

Cuts at:      1     762    1229    1252    1587    1833    2845    2931    2943 
   Size:        761     467      23     335     246    1012      86      12

Cuts at:   2943    3289       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     761     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    365     365 
   Size:       3737
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1687    1868    2031    1687 
   Size:        181     163    3393

  Fragments arranged by size: 

                3393     181     163
 
BtsI GCAGTG_nn'

Cuts at:    462     980     462 
   Size:        518    3219
 
DraI TTT'AAA

Cuts at:    736     736 
   Size:       3737
 
DrdI GACnn_nn'nnGTC

Cuts at:   1443    2301    1443 
   Size:        858    2879
 
EaeI y'GGCC_r

Cuts at:    707    1322    1496    1887    1914    3259     707 
   Size:        615     174     391      27    1345    1185

  Fragments arranged by size: 

                1345    1185     615     391     174      27
 
EagI C'GGCC_G

Cuts at:   1322    1322 
   Size:       3737
 
EarI CTCTTCn'nnn_

Cuts at:   1760    1970    1760 
   Size:        210    3527
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1945    2255    2401    3547    1945 
   Size:        310     146    1146    2135

  Fragments arranged by size: 

                2135    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    749     749 
   Size:       3737
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1562    1994    2741    3009    1562 
   Size:        432     747     268    2290

  Fragments arranged by size: 

                2290     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    876     876 
   Size:       3737
 
EcoRI G'AATT_C

Cuts at:    741     741 
   Size:       3737
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1756    1756 
   Size:       3737
 
FspI TGC'GCA

Cuts at:   1518    1518 
   Size:       3737
 
HaeII r_GCGC'y

Cuts at:   1171    1419    2441    3420    1171 
   Size:        248    1022     979    1488

  Fragments arranged by size: 

                1488    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    244     276     287     319    1579    1609    1611    1641     244 
   Size:         32      11      32    1260      30       2      30    2340

  Fragments arranged by size: 

                2340    1260      32      32      30      30      11       2
            
 
HincII GTy'rAC

Cuts at:    776     776 
   Size:       3737
 
KasI G'GCGC_C

Cuts at:   1167    1415    1167 
   Size:        248    3489
 
KpnI G_GTAC'C

Cuts at:    757     757 
   Size:       3737
 
MluI A'CGCG_T

Cuts at:   3170    3170 
   Size:       3737
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    435    2408    2592     435 
   Size:       1973     184    1580

  Fragments arranged by size: 

                1973    1580     184
 
MscI TGG'CCA

Cuts at:   1498    1498 
   Size:       3737
 
MslI CAynn'nnrTG

Cuts at:    475    1726    1853     475 
   Size:       1251     127    2359

  Fragments arranged by size: 

                2359    1251     127
 
MspA1I CmG'CkG

Cuts at:    316     848    1162    1522    2535    2780     316 
   Size:        532     314     360    1013     245    1273

  Fragments arranged by size: 

                1273    1013     532     360     314     245
 
NaeI GCC'GGC

Cuts at:   1918    1918 
   Size:       3737
 
NarI GG'CG_CC

Cuts at:   1168    1416    1168 
   Size:        248    3489
 
NgoMIV G'CCGG_C

Cuts at:   1916    1916 
   Size:       3737
 
NspI r_CATG'y

Cuts at:    790    1290    2197     790 
   Size:        500     907    2330

  Fragments arranged by size: 

                2330     907     500
 
PciI A'CATG_T

Cuts at:   2193    2193 
   Size:       3737
 
PfoI T'CCnGG_A

Cuts at:    356     647     356 
   Size:        291    3446
 
PmeI GTTT'AAAC

Cuts at:    736     736 
   Size:       3737
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2876    2908    2876 
   Size:         32    3705
 
PstI C_TGCA'G

Cuts at:    302     784    1469     302 
   Size:        482     685    2570

  Fragments arranged by size: 

                2570     685     482
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3737
 
PvuII CAG'CTG

Cuts at:    316    1162    1522     316 
   Size:        846     360    2531

  Fragments arranged by size: 

                2531     846     360
 
RsrII CG'GwC_CG

Cuts at:   1932    1932 
   Size:       3737
 
SacI G_AGCT'C

Cuts at:    751     751 
   Size:       3737
 
SalI G'TCGA_C

Cuts at:    774     774 
   Size:       3737
 
SapI GCTCTTCn'nnn_

Cuts at:   1760    1970    1760 
   Size:        210    3527
 
SbfI CC_TGCA'GG

Cuts at:    784     784 
   Size:       3737
 
ScaI AGT'ACT

Cuts at:   3254    3254 
   Size:       3737
 
SfcI C'TryA_G

Cuts at:     33     298     780    1465    2458    2649      33 
   Size:        265     482     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     482     265     191
 
SfoI GGC'GCC

Cuts at:   1169    1417    1169 
   Size:        248    3489
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3737
 
SmaI CCC'GGG

Cuts at:    759     759 
   Size:       3737
 
SmlI C'TyrA_G

Cuts at:    892    1807    2299    2561     892 
   Size:        915     492     262    2068

  Fragments arranged by size: 

                2068     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3737
 
SphI G_CATG'C

Cuts at:    790    1290     790 
   Size:        500    3237
 
StyI C'CwwG_G

Cuts at:    871    1225     871 
   Size:        354    3383
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1320    1453    1912    2121    1320 
   Size:        133     459     209    2936

  Fragments arranged by size: 

                2936     459     209     133
 
TatI w'GTAC_w

Cuts at:    272    3252     272 
   Size:       2980     757
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3254    3286    3254 
   Size:         32    3705
 
Tth111I GACn'n_nGTC

Cuts at:    436    1534     436 
   Size:       1098    2639
 
XbaI T'CTAG_A

Cuts at:    768     768 
   Size:       3737
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    241     372    1232     241 
   Size:        131     860    2746

  Fragments arranged by size: 

                2746     860     131
 
XmaI C'CCGG_G

Cuts at:    757     757 
   Size:       3737
 
ZraI GAC'GTC

Cuts at:    188     438     188 
   Size:        250    3487
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PfoI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI    BbvCI    BfrBI     BglI     BmtI     BpmI   Bpu10I
    BsaXI     BsgI    BsiWI     BsmI    BsrGI   BstEII  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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