Restriction map for pF1KB3619
 (Circular) (Six-base) MAPSORT of: pF1KB3619.seq  Check: 7675  from: 1  to: 5819



pF1KB3619  5819 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:   2857    2857 
   Size:       5819
 
Acc65I G'GTAC_C

Cuts at:    506    1736    2835     506 
   Size:       1230    1099    3490

  Fragments arranged by size: 

                3490    1230    1099
 
AclI AA'CG_TT

Cuts at:   5659    5659 
   Size:       5819
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    279    1426    2290    2495    3644    4076    4429    5091     279 
   Size:       1147     864     205    1149     432     353     662    1007

  Fragments arranged by size: 

                1149    1147    1007     864     662     432     353     205
            
 
AflIII A'CryG_T

Cuts at:   1245    3171    4971    5252    1245 
   Size:       1926    1800     281    1812

  Fragments arranged by size: 

                1926    1812    1800     281
 
AgeI A'CCGG_T

Cuts at:   1562    1562 
   Size:       5819
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1510    1542    2359    2391    1510 
   Size:         32     817      32    4938

  Fragments arranged by size: 

                4938     817      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    353    3124    4562     353 
   Size:       2771    1438    1610

  Fragments arranged by size: 

                2771    1610    1438
 
ApaLI G'TGCA_C

Cuts at:   4657    4657 
   Size:       5819
 
ApoI r'AATT_y

Cuts at:    359     585     603    1456    1610    2653    2823    5172    5428 
   Size:        226      18     853     154    1043     170    2349     256

Cuts at:   5428     359 
   Size:        750

  Fragments arranged by size: 

                2349    1043     853     750     256     226     170     154
                  18
 
AseI AT'TA_AT

Cuts at:     20    1427      20 
   Size:       1407    4412
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5819
 
AvaI C'yCGr_G

Cuts at:   2052    2839    2052 
   Size:        787    5032
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1104    1137    1720    1753    1104 
   Size:         33     583      33    5170

  Fragments arranged by size: 

                5170     583      33      33
 
BamHI G'GATC_C

Cuts at:   2844    2844 
   Size:       5819
 
BanI G'GyrC_C

Cuts at:    506    1736    2287    2835    3249    3497    3532     506 
   Size:       1230     551     548     414     248      35    2793

  Fragments arranged by size: 

                2793    1230     551     548     414     248      35
 
BanII G_rGCy'C

Cuts at:    135     191    2405    2833    3863     135 
   Size:         56    2214     428    1030    2091

  Fragments arranged by size: 

                2214    2091    1030     428      56
 
BbeI G_GCGC'C

Cuts at:   3253    3501    3253 
   Size:        248    5571
 
BbsI GAAGACnn'nnnn_

Cuts at:    572    2237    2701    2816     572 
   Size:       1665     464     115    3575

  Fragments arranged by size: 

                3575    1665     464     115
 
BciVI GTATCCnnnnn_n'

Cuts at:   2421    3717    4773    5819    2421 
   Size:       1296    1056    1046    2421

  Fragments arranged by size: 

                2421    1296    1056    1046
 
BclI T'GATC_A

Cuts at:   3339    3339 
   Size:       5819
 
BglII A'GATC_T

Cuts at:   2321    3334    5371    2321 
   Size:       1013    2037    2769

  Fragments arranged by size: 

                2769    2037    1013
 
BlpI GC'TnA_GC

Cuts at:   2931    2931 
   Size:       5819
 
Bme1580I G_kGCm'C

Cuts at:   3444    3537    4661    3444 
   Size:         93    1124    4602

  Fragments arranged by size: 

                4602    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    632    3031    3112    3447    5591     632 
   Size:       2399      81     335    2144     860

  Fragments arranged by size: 

                2399    2144     860     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4186    4186 
   Size:       5819
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    854    2389     854 
   Size:       1535    4284
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    972    2995    3874    4588    4886     972 
   Size:       2023     879     714     298    1905

  Fragments arranged by size: 

                2023    1905     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    778    2292    5523     778 
   Size:       1514    3231    1074

  Fragments arranged by size: 

                3231    1514    1074
 
BsaAI yAC'GTr

Cuts at:    679    1248    3172     679 
   Size:        569    1924    3326

  Fragments arranged by size: 

                3326    1924     569
 
BsaBI GATnn'nnATC

Cuts at:      6     908    3333    5370       6 
   Size:        902    2425    2037     455

  Fragments arranged by size: 

                2425    2037     902     455
 
BsaHI Gr'CG_yC

Cuts at:   3250    3498    4200    5696    3250 
   Size:        248     702    1496    3373

  Fragments arranged by size: 

                3373    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1562    1739    3008    3529    4207    4618    4765    5039    1562 
   Size:        177    1269     521     678     411     147     274    2342

  Fragments arranged by size: 

                2342    1269     678     521     411     274     177     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1813    1843    2359    2389    1813 
   Size:         30     516      30    5243

  Fragments arranged by size: 

                5243     516      30      30
 
BseYI C'CCAG_C

Cuts at:    134    2588    3244    4002    4667    5075     134 
   Size:       2454     656     758     665     408     878

  Fragments arranged by size: 

                2454     878     758     665     656     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1226    1226 
   Size:       5819
 
BsiEI CG_ry'CG

Cuts at:    104     128    3361    3407    4637     104 
   Size:         24    3233      46    1230    1286

  Fragments arranged by size: 

                3233    1286    1230      46      24
 
BsiHKAI G_wGCw'C

Cuts at:    958    2405    2833    3611    3801    4661     958 
   Size:       1447     428     778     190     860    2116

  Fragments arranged by size: 

                2116    1447     860     778     428     190
 
BsmI GAATG_Cn'

Cuts at:   1026    2396    2435    2632    1026 
   Size:       1370      39     197    4213

  Fragments arranged by size: 

                4213    1370     197      39
 
Bsp1286I G_dGCh'C

Cuts at:    135     191     958    2405    2833    3444    3537    3611    3801 
   Size:         56     767    1447     428     611      93      74     190

Cuts at:   3801    3863    4661     135 
   Size:         62     798    1293

  Fragments arranged by size: 

                1447    1293     798     767     611     428     190      93
                  74      62      56
 
BspHI T'CATG_A

Cuts at:   1343    4251    1343 
   Size:       2908    2911
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2869    3083    3385    3766    2869 
   Size:        214     302     381    4922

  Fragments arranged by size: 

                4922     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     120    4111    4165    5649    5814      50 
   Size:         70    3991      54    1484     165      55

  Fragments arranged by size: 

                3991    1484     165      70      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3731    3731 
   Size:       5819
 
BsrFI r'CCGG_y

Cuts at:   1562    3817    3998    1562 
   Size:       2255     181    3383

  Fragments arranged by size: 

                3383    2255     181
 
BssSI C'ACGA_G

Cuts at:   4090    4798    4090 
   Size:        708    5111
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3196    3196 
   Size:       5819
 
BstBI TT'CG_AA

Cuts at:   4180    4180 
   Size:       5819
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2386    2736    2386 
   Size:        350    5469
 
BstYI r'GATC_y

Cuts at:      1    2321    2567    2844    3311    3334    3669    3915    4221 
   Size:       2320     246     277     467      23     335     246     306

Cuts at:   4221    4233    4319    5371       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                2320    1052     467     449     335     306     277     246
                 246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2716    2716 
   Size:       5819
 
BtgI C'CryG_G

Cuts at:    107    1004    1948     107 
   Size:        897     944    3978

  Fragments arranged by size: 

                3978     944     897
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2553    3769    3950    4113    2553 
   Size:       1216     181     163    4259

  Fragments arranged by size: 

                4259    1216     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2479    3062    2479 
   Size:        583    5236
 
DraI TTT'AAA

Cuts at:   2818    2818 
   Size:       5819
 
DraIII CAC_nnn'GTG

Cuts at:   2469    2469 
   Size:       5819
 
DrdI GACnn_nn'nnGTC

Cuts at:   3525    4869    3525 
   Size:       1344    4475
 
EaeI y'GGCC_r

Cuts at:    861    3404    3578    3969    3996    5341     861 
   Size:       2543     174     391      27    1345    1339

  Fragments arranged by size: 

                2543    1345    1339     391     174      27
 
EagI C'GGCC_G

Cuts at:   3404    3404 
   Size:       5819
 
EarI CTCTTCn'nnn_

Cuts at:   1705    2393    2704    3842    4052    1705 
   Size:        688     311    1138     210    3472

  Fragments arranged by size: 

                3472    1138     688     311     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4027    4769    4915    5629    4027 
   Size:        742     146     714    4217

  Fragments arranged by size: 

                4217     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2403    2831    2403 
   Size:        428    5391
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    279     854    1426    2290    2389    2495    3644    4076    4429 
   Size:        575     572     864      99     106    1149     432     353

Cuts at:   4429    5091     279 
   Size:        662    1007

  Fragments arranged by size: 

                1149    1007     864     662     575     572     432     353
                 106      99
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2245    2245 
   Size:       5819
 
EcoO109I rG'GnC_Cy

Cuts at:    175    2370    2958     175 
   Size:       2195     588    3036

  Fragments arranged by size: 

                3036    2195     588
 
EcoRI G'AATT_C

Cuts at:   1610    2823    1610 
   Size:       1213    4606
 
EcoRV GAT'ATC

Cuts at:    273     921     273 
   Size:        648    5171
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    340    3838     340 
   Size:       3498    2321
 
FspI TGC'GCA

Cuts at:   3600    3600 
   Size:       5819
 
HaeII r_GCGC'y

Cuts at:    393    1697    3253    3501    4731    5502     393 
   Size:       1304    1556     248    1230     771     710

  Fragments arranged by size: 

                1556    1304    1230     771     710     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    159     191    1254    1286    2567    2599    2679    2711    3661 
   Size:         32    1063      32    1281      32      80      32     950

Cuts at:   3661    3691    3693    3723     159 
   Size:         30       2      30    2255

  Fragments arranged by size: 

                2255    1281    1063     950      80      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:    816    1503    2858     816 
   Size:        687    1355    3777

  Fragments arranged by size: 

                3777    1355     687
 
HindIII A'AGCT_T

Cuts at:    689    1493     689 
   Size:        804    5015
 
KasI G'GCGC_C

Cuts at:   3249    3497    3249 
   Size:        248    5571
 
KpnI G_GTAC'C

Cuts at:    510    1740    2839     510 
   Size:       1230    1099    3490

  Fragments arranged by size: 

                3490    1230    1099
 
MluI A'CGCG_T

Cuts at:   5252    5252 
   Size:       5819
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    108     822    1122    4578    4762     108 
   Size:        714     300    3456     184    1165

  Fragments arranged by size: 

                3456    1165     714     300     184
 
MscI TGG'CCA

Cuts at:    863    3580     863 
   Size:       2717    3102
 
MslI CAynn'nnrTG

Cuts at:   2609    3808    3935    2609 
   Size:       1199     127    4493

  Fragments arranged by size: 

                4493    1199     127
 
MspA1I CmG'CkG

Cuts at:    439     765    2930    3244    3604    4388    4633     439 
   Size:        326    2165     314     360     784     245    1625

  Fragments arranged by size: 

                2165    1625     784     360     326     314     245
 
NaeI GCC'GGC

Cuts at:   4000    4000 
   Size:       5819
 
NarI GG'CG_CC

Cuts at:   3250    3498    3250 
   Size:        248    5571
 
NcoI C'CATG_G

Cuts at:    107    1004    1948     107 
   Size:        897     944    3978

  Fragments arranged by size: 

                3978     944     897
 
NgoMIV G'CCGG_C

Cuts at:   3998    3998 
   Size:       5819
 
NspI r_CATG'y

Cuts at:    265    1398    2872    3372    4975     265 
   Size:       1133    1474     500    1603    1109

  Fragments arranged by size: 

                1603    1474    1133    1109     500
 
PciI A'CATG_T

Cuts at:   4971    4971 
   Size:       5819
 
PflMI CCAn_nnn'nTGG

Cuts at:    945    2469     945 
   Size:       1524    4295
 
PfoI T'CCnGG_A

Cuts at:    182     182 
   Size:       5819
 
PmeI GTTT'AAAC

Cuts at:   2818    2818 
   Size:       5819
 
PmlI CAC'GTG

Cuts at:    679    1248     679 
   Size:        569    5250
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2359    2391    4265    4297    2359 
   Size:         32    1874      32    3881

  Fragments arranged by size: 

                3881    1874      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    175    2370     175 
   Size:       2195    3624
 
PsiI TTA'TAA

Cuts at:   1280    1541    1280 
   Size:        261    5558
 
PstI C_TGCA'G

Cuts at:   2866    3551    2866 
   Size:        685    5134
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5819
 
PvuII CAG'CTG

Cuts at:    439    3244    3604     439 
   Size:       2805     360    2654

  Fragments arranged by size: 

                2805    2654     360
 
RsrII CG'GwC_CG

Cuts at:   4014    4014 
   Size:       5819
 
SacI G_AGCT'C

Cuts at:   2405    2833    2405 
   Size:        428    5391
 
SalI G'TCGA_C

Cuts at:   2856    2856 
   Size:       5819
 
SapI GCTCTTCn'nnn_

Cuts at:   2393    3842    4052    2393 
   Size:       1449     210    4160

  Fragments arranged by size: 

                4160    1449     210
 
SbfI CC_TGCA'GG

Cuts at:   2866    2866 
   Size:       5819
 
ScaI AGT'ACT

Cuts at:   1939    5336    1939 
   Size:       3397    2422
 
SfcI C'TryA_G

Cuts at:     33    1010    1130    2862    3547    4515    4706      33 
   Size:        977     120    1732     685     968     191    1146

  Fragments arranged by size: 

                1732    1146     977     968     685     191     120
 
SfoI GGC'GCC

Cuts at:   3251    3499    3251 
   Size:        248    5571
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5819
 
SmaI CCC'GGG

Cuts at:   2841    2841 
   Size:       5819
 
SmlI C'TyrA_G

Cuts at:    951    2974    3889    4603    4865     951 
   Size:       2023     915     714     262    1905

  Fragments arranged by size: 

                2023    1905     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67    2704      67 
   Size:       2637    3182
 
SphI G_CATG'C

Cuts at:   1398    2872    3372    1398 
   Size:       1474     500    3845

  Fragments arranged by size: 

                3845    1474     500
 
SspI AAT'ATT

Cuts at:   1473    1473 
   Size:       5819
 
StuI AGG'CCT

Cuts at:   2721    2721 
   Size:       5819
 
StyI C'CwwG_G

Cuts at:    107     889    1004    1948    2315    2953    3307     107 
   Size:        782     115     944     367     638     354    2619

  Fragments arranged by size: 

                2619     944     782     638     367     354     115
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1622    2742    3402    3535    3994    4203    1622 
   Size:       1120     660     133     459     209    3238

  Fragments arranged by size: 

                3238    1120     660     459     209     133
 
TatI w'GTAC_w

Cuts at:   1937    5334    1937 
   Size:       3397    2422
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5336    5368    5336 
   Size:         32    5787
 
Tth111I GACn'n_nGTC

Cuts at:   3616    3616 
   Size:       5819
 
XbaI T'CTAG_A

Cuts at:   2850    2850 
   Size:       5819
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3314    3314 
   Size:       5819
 
XmaI C'CCGG_G

Cuts at:   2839    2839 
   Size:       5819
 
XmnI GAAnn'nnTTC

Cuts at:   2075    2075 
   Size:       5819
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
   Bpu10I    BseRI    BsiWI    BsmBI    BspEI    BsrGI   BssHII   BstEII
  BstZ17I     ClaI    CspCI     FseI    FspAI     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PshAI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com