Restriction map for pF1KB3567
 (Circular) (Six-base) MAPSORT of: pF1KB3567.seq  Check: 7914  from: 1  to: 5255



pF1KB3567  5255 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    939     939 
   Size:       5255
 
AccI GT'mk_AC

Cuts at:   1442    2117    2293    1442 
   Size:        675     176    4404

  Fragments arranged by size: 

                4404     675     176
 
Acc65I G'GTAC_C

Cuts at:    297    2271     297 
   Size:       1974    3281
 
AclI AA'CG_TT

Cuts at:   5095    5095 
   Size:       5255
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1743    1845    3080    3512    3865    4527    1743 
   Size:        102    1235     432     353     662    2471

  Fragments arranged by size: 

                2471    1235     662     432     353     102
 
AflIII A'CryG_T

Cuts at:   2607    4407    4688    2607 
   Size:       1800     281    3174

  Fragments arranged by size: 

                3174    1800     281
 
AhdI GACnn_n'nnGTC

Cuts at:   1347    1347 
   Size:       5255
 
AleI CACnn'nnGTG

Cuts at:    130     145     130 
   Size:         15    5240
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1381    1381 
   Size:       5255
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1090    1122    1090 
   Size:         32    5223
 
AlwNI CAG_nnn'CTG

Cuts at:    309    2560    3998     309 
   Size:       2251    1438    1566

  Fragments arranged by size: 

                2251    1566    1438
 
ApaLI G'TGCA_C

Cuts at:    640    4093     640 
   Size:       3453    1802
 
ApoI r'AATT_y

Cuts at:   2259    4608    4864    2259 
   Size:       2349     256    2650

  Fragments arranged by size: 

                2650    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5255
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5255
 
AvaI C'yCGr_G

Cuts at:   1517    1579    2275    1517 
   Size:         62     696    4497

  Fragments arranged by size: 

                4497     696      62
 
BamHI G'GATC_C

Cuts at:   2280    2280 
   Size:       5255
 
BanI G'GyrC_C

Cuts at:    297    1225    1596    2271    2685    2933    2968     297 
   Size:        928     371     675     414     248      35    2584

  Fragments arranged by size: 

                2584     928     675     414     371     248      35
 
BanII G_rGCy'C

Cuts at:    844    1616    2081    2105    2269    3299     844 
   Size:        772     465      24     164    1030    2800

  Fragments arranged by size: 

                2800    1030     772     465     164      24
 
BbeI G_GCGC'C

Cuts at:   2689    2937    2689 
   Size:        248    5007
 
BciVI GTATCCnnnnn_n'

Cuts at:   3153    4209    5255    3153 
   Size:       1056    1046    3153

  Fragments arranged by size: 

                3153    1056    1046
 
BclI T'GATC_A

Cuts at:   2775    2775 
   Size:       5255
 
BglI GCCn_nnn'nGGC

Cuts at:   1409    1409 
   Size:       5255
 
BglII A'GATC_T

Cuts at:   1154    2190    2770    4807    1154 
   Size:       1036     580    2037    1602

  Fragments arranged by size: 

                2037    1602    1036     580
 
BlpI GC'TnA_GC

Cuts at:   2367    2367 
   Size:       5255
 
Bme1580I G_kGCm'C

Cuts at:    644    1601    2880    2973    4097     644 
   Size:        957    1279      93    1124    1802

  Fragments arranged by size: 

                1802    1279    1124     957      93
 
BmgBI CAC'GTC

Cuts at:    591     591 
   Size:       5255
 
BmrI ACTGGGnnnn_n'

Cuts at:    152     387     451     794    2467    2548    2883    5027     152 
   Size:        235      64     343    1673      81     335    2144     380

  Fragments arranged by size: 

                2144    1673     380     343     335     235      81      64
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    858    3622     858 
   Size:       2764    2491
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    687     853     855    1047    1171     687 
   Size:        166       2     192     124    4771

  Fragments arranged by size: 

                4771     192     166     124       2
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    700     808    1165    2431    3310    4024    4322     700 
   Size:        108     357    1266     879     714     298    1633

  Fragments arranged by size: 

                1633    1266     879     714     357     298     108
 
BsaI GGTCTCn'nnnn_

Cuts at:   2149    4959    2149 
   Size:       2810    2445
 
BsaAI yAC'GTr

Cuts at:   2608    2608 
   Size:       5255
 
BsaBI GATnn'nnATC

Cuts at:      6    2769    4806       6 
   Size:       2763    2037     455

  Fragments arranged by size: 

                2763    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1175    2686    2934    3636    5132    1175 
   Size:       1511     248     702    1496    1298

  Fragments arranged by size: 

                1511    1496    1298     702     248
 
BsaWI w'CCGG_w

Cuts at:    983    1455    1958    1979    2444    2965    3643    4054    4201 
   Size:        472     503      21     465     521     678     411     147

Cuts at:   4201    4475     983 
   Size:        274    1763

  Fragments arranged by size: 

                1763     678     521     503     472     465     411     274
                 147      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    130    1029    1744     130 
   Size:        899     715    3641

  Fragments arranged by size: 

                3641     899     715
 
BseYI C'CCAG_C

Cuts at:    183     849    1269    1663    1920    2680    3438    4103    4511 
   Size:        666     420     394     257     760     758     665     408

Cuts at:   4511     183 
   Size:        927

  Fragments arranged by size: 

                 927     760     758     666     665     420     408     394
                 257
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    123     181     484     123 
   Size:         58     303    4894

  Fragments arranged by size: 

                4894     303      58
 
BsiEI CG_ry'CG

Cuts at:    104    1413    1478    2117    2797    2843    4073     104 
   Size:       1309      65     639     680      46    1230    1286

  Fragments arranged by size: 

                1309    1286    1230     680     639      65      46
 
BsiHKAI G_wGCw'C

Cuts at:    441     644    1246    1370    1721    2081    2269    3047    3237 
   Size:        203     602     124     351     360     188     778     190

Cuts at:   3237    4097     441 
   Size:        860    1599

  Fragments arranged by size: 

                1599     860     778     602     360     351     203     190
                 188     124
 
BsmI GAATG_Cn'

Cuts at:    210     210 
   Size:       5255
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1309    1520    1309 
   Size:        211    5044
 
Bsp1286I G_dGCh'C

Cuts at:    441     644     844    1246    1370    1601    1616    1721    2081 
   Size:        203     200     402     124     231      15     105     360

Cuts at:   2081    2105    2269    2880    2973    3047    3237    3299    4097 
   Size:         24     164     611      93      74     190      62     798

Cuts at:   4097     441 
   Size:       1599

  Fragments arranged by size: 

                1599     798     611     402     360     231     203     200
                 190     164     124     105      93      74      62      24
                  15
 
BspEI T'CCGG_A

Cuts at:    983    1455    1958    1979     983 
   Size:        472     503      21    4259

  Fragments arranged by size: 

                4259     503     472      21
 
BspHI T'CATG_A

Cuts at:   3687    3687 
   Size:       5255
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    614     939    1553    1853    2305    2519    2821    3202     614 
   Size:        325     614     300     452     214     302     381    2667

  Fragments arranged by size: 

                2667     614     452     381     325     302     300     214
            
 
BsrBI CCG'CTC

Cuts at:     50     837    3547    3601    5085    5250      50 
   Size:        787    2710      54    1484     165      55

  Fragments arranged by size: 

                2710    1484     787     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    467    1917    3167     467 
   Size:       1450    1250    2555

  Fragments arranged by size: 

                2555    1450    1250
 
BsrFI r'CCGG_y

Cuts at:    129     544    1473    3253    3434     129 
   Size:        415     929    1780     181    1950

  Fragments arranged by size: 

                1950    1780     929     415     181
 
BssHII G'CGCG_C

Cuts at:   1522    1522 
   Size:       5255
 
BssSI C'ACGA_G

Cuts at:   3526    4234    3526 
   Size:        708    4547
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2632    2632 
   Size:       5255
 
BstBI TT'CG_AA

Cuts at:   3616    3616 
   Size:       5255
 
BstEII G'GTnAC_C

Cuts at:    768     768 
   Size:       5255
 
BstXI CCAn_nnnn'nTGG

Cuts at:    183     183 
   Size:       5255
 
BstYI r'GATC_y

Cuts at:      1    1154    1301    1688    2190    2280    2747    2770    3105 
   Size:       1153     147     387     502      90     467      23     335

Cuts at:   3105    3351    3657    3669    3755    4807       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1153    1052     502     467     449     387     335     306
                 246     147      90      86      23      12
 
BtgI C'CryG_G

Cuts at:    371    1247     371 
   Size:        876    4379
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    264    3205    3386    3549     264 
   Size:       2941     181     163    1970

  Fragments arranged by size: 

                2941    1970     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2498    2498 
   Size:       5255
 
DraI TTT'AAA

Cuts at:   2254    2254 
   Size:       5255
 
DraIII CAC_nnn'GTG

Cuts at:    507     507 
   Size:       5255
 
DrdI GACnn_nn'nnGTC

Cuts at:   2113    2961    4305    2113 
   Size:        848    1344    3063

  Fragments arranged by size: 

                3063    1344     848
 
EaeI y'GGCC_r

Cuts at:    464     519    1410    1475    1505    2033    2840    3014    3405 
   Size:         55     891      65      30     528     807     174     391

Cuts at:   3405    3432    4777     464 
   Size:         27    1345     942

  Fragments arranged by size: 

                1345     942     891     807     528     391     174      65
                  55      30      27
 
EagI C'GGCC_G

Cuts at:   1410    1475    2840    1410 
   Size:         65    1365    3825

  Fragments arranged by size: 

                3825    1365      65
 
EarI CTCTTCn'nnn_

Cuts at:    277    1651    1721    1762    3278    3488     277 
   Size:       1374      70      41    1516     210    2044

  Fragments arranged by size: 

                2044    1516    1374     210      70      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1558    3463    4205    4351    5065    1558 
   Size:       1905     742     146     714    1748

  Fragments arranged by size: 

                1905    1748     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2079    2267    2079 
   Size:        188    5067
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    687     853     855    1047    1171    1743    1845    3080    3512 
   Size:        166       2     192     124     572     102    1235     432

Cuts at:   3512    3865    4527     687 
   Size:        353     662    1415

  Fragments arranged by size: 

                1415    1235     662     572     432     353     192     166
                 124     102       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:    223    1549     223 
   Size:       1326    3929
 
EcoO109I rG'GnC_Cy

Cuts at:    266     321     328     717    1449    1752    2394     266 
   Size:         55       7     389     732     303     642    3127

  Fragments arranged by size: 

                3127     732     642     389     303      55       7
 
EcoRI G'AATT_C

Cuts at:   2259    2259 
   Size:       5255
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1512    1773    3274    1512 
   Size:        261    1501    3493

  Fragments arranged by size: 

                3493    1501     261
 
FspI TGC'GCA

Cuts at:    790    3036     790 
   Size:       2246    3009
 
HaeII r_GCGC'y

Cuts at:    674     832    1404    2689    2937    4167    4938     674 
   Size:        158     572    1285     248    1230     771     991

  Fragments arranged by size: 

                1285    1230     991     771     572     248     158
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    706     738    1024    1056    1333    1365    3097    3127    3129 
   Size:         32     286      32     277      32    1732      30       2

Cuts at:   3129    3159     706 
   Size:         30    2802

  Fragments arranged by size: 

                2802    1732     286     277      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   2118    2294    2118 
   Size:        176    5079
 
KasI G'GCGC_C

Cuts at:   2685    2933    2685 
   Size:        248    5007
 
KpnI G_GTAC'C

Cuts at:    301    2275     301 
   Size:       1974    3281
 
MluI A'CGCG_T

Cuts at:   4688    4688 
   Size:       5255
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:     92    4014    4198      92 
   Size:       3922     184    1149

  Fragments arranged by size: 

                3922    1149     184
 
MscI TGG'CCA

Cuts at:   1507    2035    3016    1507 
   Size:        528     981    3746

  Fragments arranged by size: 

                3746     981     528
 
MslI CAynn'nnrTG

Cuts at:    130     145     203    3244    3371     130 
   Size:         15      58    3041     127    2014

  Fragments arranged by size: 

                3041    2014     127      58      15
 
MspA1I CmG'CkG

Cuts at:    171     240     564    1249    1767    2235    2366    2680    3040 
   Size:         69     324     685     518     468     131     314     360

Cuts at:   3040    3824    4069     171 
   Size:        784     245    1357

  Fragments arranged by size: 

                1357     784     685     518     468     360     324     314
                 245     131      69
 
NaeI GCC'GGC

Cuts at:    546    1475    3436     546 
   Size:        929    1961    2365

  Fragments arranged by size: 

                2365    1961     929
 
NarI GG'CG_CC

Cuts at:   2686    2934    2686 
   Size:        248    5007
 
NdeI CA'TA_TG

Cuts at:   1065    1065 
   Size:       5255
 
NgoMIV G'CCGG_C

Cuts at:    544    1473    3434     544 
   Size:        929    1961    2365

  Fragments arranged by size: 

                2365    1961     929
 
NspI r_CATG'y

Cuts at:   2308    2808    4411    2308 
   Size:        500    1603    3152

  Fragments arranged by size: 

                3152    1603     500
 
PasI CC'CwG_GG

Cuts at:    998     998 
   Size:       5255
 
PciI A'CATG_T

Cuts at:   4407    4407 
   Size:       5255
 
PflMI CCAn_nnn'nTGG

Cuts at:    309    1280     309 
   Size:        971    4284
 
PmeI GTTT'AAAC

Cuts at:   2254    2254 
   Size:       5255
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2146    2178    3701    3733    2146 
   Size:         32    1523      32    3668

  Fragments arranged by size: 

                3668    1523      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    266     321     328    1449     266 
   Size:         55       7    1121    4072

  Fragments arranged by size: 

                4072    1121      55       7
 
PshAI GACnn'nnGTC

Cuts at:    326     326 
   Size:       5255
 
PstI C_TGCA'G

Cuts at:    260    1360    2302    2987     260 
   Size:       1100     942     685    2528

  Fragments arranged by size: 

                2528    1100     942     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5255
 
PvuII CAG'CTG

Cuts at:    240     564    1767    2235    2680    3040     240 
   Size:        324    1203     468     445     360    2455

  Fragments arranged by size: 

                2455    1203     468     445     360     324
 
RsrII CG'GwC_CG

Cuts at:   3450    3450 
   Size:       5255
 
SacI G_AGCT'C

Cuts at:   2081    2269    2081 
   Size:        188    5067
 
SacII CC_GC'GG

Cuts at:   1250    1250 
   Size:       5255
 
SalI G'TCGA_C

Cuts at:   2116    2292    2116 
   Size:        176    5079
 
SanDI GG'GwC_CC

Cuts at:    266     328     266 
   Size:         62    5193
 
SapI GCTCTTCn'nnn_

Cuts at:   3278    3488    3278 
   Size:        210    5045
 
SbfI CC_TGCA'GG

Cuts at:   2302    2302 
   Size:       5255
 
ScaI AGT'ACT

Cuts at:   4772    4772 
   Size:       5255
 
SfcI C'TryA_G

Cuts at:     33     256    1356    2298    2983    3951    4142      33 
   Size:        223    1100     942     685     968     191    1146

  Fragments arranged by size: 

                1146    1100     968     942     685     223     191
 
SfoI GGC'GCC

Cuts at:   2687    2935    2687 
   Size:        248    5007
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5255
 
SgrAI Cr'CCGG_yG

Cuts at:    129     129 
   Size:       5255
 
SmaI CCC'GGG

Cuts at:   1581    2277    1581 
   Size:        696    4559
 
SmlI C'TyrA_G

Cuts at:    679     823    1180    2410    3325    4039    4301     679 
   Size:        144     357    1230     915     714     262    1633

  Fragments arranged by size: 

                1633    1230     915     714     357     262     144
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5255
 
SphI G_CATG'C

Cuts at:   2308    2808    2308 
   Size:        500    4755
 
StuI AGG'CCT

Cuts at:    286     688     286 
   Size:        402    4853
 
StyI C'CwwG_G

Cuts at:    695    1853    2389    2743     695 
   Size:       1158     536     354    3207

  Fragments arranged by size: 

                3207    1158     536     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    573    1272    2838    2971    3430    3639     573 
   Size:        699    1566     133     459     209    2189

  Fragments arranged by size: 

                2189    1566     699     459     209     133
 
TatI w'GTAC_w

Cuts at:    134    4770     134 
   Size:       4636     619
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4772    4804    4772 
   Size:         32    5223
 
Tth111I GACn'n_nGTC

Cuts at:    592    3052     592 
   Size:       2460    2795
 
XbaI T'CTAG_A

Cuts at:    879    2286     879 
   Size:       1407    3848
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    216    1277    2750     216 
   Size:       1061    1473    2721

  Fragments arranged by size: 

                2721    1473    1061
 
XmaI C'CCGG_G

Cuts at:   1579    2275    1579 
   Size:        696    4559
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     ApaI     AscI    AvrII     BaeI
     BbsI    BbvCI     BcgI    BfrBI     BmtI   Bpu10I    BsaXI    BsiWI
    BsrGI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NcoI     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmlI     PsiI   PspOMI    PspXI     PsrI    SexAI
     SfiI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com