Restriction map for pF1KB3373
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BglI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoT22I     
FauI        FseI        FspI        HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SbfI        ScaI        SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI         1675  CCGC                 CCGC(-3/-1)
AflIII       1838  ACATGT               A^C[GA][CT]GT
AhdI         1527  GACCATCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1527  GACCATCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1052  GAGCTC               G[AT]GC[AT]^C
AvrII         703  CCTAGG               C^CTAGG
BalI         1086  TGGCCA               TGG^CCA
BcnI          533  CCCGG                CC^[GC]GG
BlnI          703  CCTAGG               C^CTAGG
BsaBI         724  GATAATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      1052  GAGCTC               G[AT]GC[AT]^C
BsmI          698  GAATGC               GAATGC(1/-1)
BspLU11I     1838  ACATGT               A^CATGT
BstAPI       1696  GCAATGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1301  CCATATTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI         1711  TTTAAA               TTT^AAA
EaeI         1086  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1527  GACCATCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1447  GATATC               GAT^ATC
HaeII        1357  GGCGCT               [GA]GCGC^[CT]
HapII         534  CCGG                 C^CGG
HincII         62  GTCGAC               GT[CT]^[GA]AC
HindII         62  GTCGAC               GT[CT]^[GA]AC
HindIII        14  AAGCTT               A^AGCTT
HpaII         534  CCGG                 C^CGG
MaeII        1487  ACGT                 A^CGT
MamI          724  GATAATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1086  TGGCCA               TGG^CCA
MscI         1086  TGGCCA               TGG^CCA
MspA1I       1817  CAGCTG               C[AC]G^C[GT]G
MspI          534  CCGG                 C^CGG
NciI          533  CCCGG                CC^[GC]GG
PflMI         594  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          134  GAGTC                GAGTC(4/5)
PvuII        1817  CAGCTG               CAG^CTG
SacI         1052  GAGCTC               GAGCT^C
SalI           62  GTCGAC               G^TCGAC
SapI          336  GAAGAGC              GCTCTTC(1/4)
SexAI         524  ACCAGGT              A^CC[AT]GGT
Van91I        594  CCAAATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI           62  GTCGAC               GT^[AC][GT]AC
             1108  GTCTAC              
AfaI          371  GTAC                 GT^AC
             1646  GTAC                
AflII         921  CTTAAG               C^TTAAG
             1830  CTTAAG              
AlwNI        1222  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1396  CAGATTCTG           
AvaI         1049  CTCGAG               C^[CT]CG[GA]G
             1769  CTCGAG              
BamHI         603  GGATCC               G^GATCC
             1278  GGATCC              
BclI            6  TGATCA               T^GATCA
              912  TGATCA              
BfrI          921  CTTAAG               C^TTAAG
             1830  CTTAAG              
BglII         198  AGATCT               A^GATCT
              205  AGATCT              
BseRI         491  CTCCTC               GAGGAG(10/8)
              152  GAGGAG              
BsmFI         874  GGGAC                GGGAC(10/14)
             1543  GGGAC               
BsoBI        1049  CTCGAG               C^[CT]CG[GA]G
             1769  CTCGAG              
BsrDI         395  CATTGC               GCAATG(2/0)
             1696  GCAATG              
Cac8I          12  GCAAGC               GC[ACGT]^[ACGT]GC
             1510  GCCAGC              
CfoI         1358  GCGC                 GCG^C
             1457  GCGC                
DraII         465  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              865  AGGACCC             
EarI          336  GAAGAG               CTCTTC(1/4)
             1136  GAAGAG              
EcoO109I      465  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              865  AGGACCC             
FbaI            6  TGATCA               T^GATCA
              912  TGATCA              
HhaI         1358  GCGC                 GCG^C
             1457  GCGC                
HinP1I       1358  GCGC                 G^CGC
             1457  GCGC                
HphI          109  TCACC                GGTGA(8/7)
             1045  TCACC               
Ksp632I       336  GAAGAG               CTCTTC(1/4)
             1136  GAAGAG              
NdeI          331  CATATG               CA^TATG
             1352  CATATG              
NspI         1642  GCATGT               [GA]CATG^[CT]
             1838  ACATGT              
PaeR7I       1049  CTCGAG               C^TCGAG
             1769  CTCGAG              
PpuMI         465  AGGTCCT              [GA]G^G[AT]CC[CT]
              865  AGGACCC             
RsaI          371  GTAC                 GT^AC
             1646  GTAC                
TspRI         561  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1599  CAGTG               
XhoI         1049  CTCGAG               C^TCGAG
             1769  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        AvaII       
BanII       BbvI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       EcoNI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SmlI        SpeI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TthHB8I     XcmI        XhoII       
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