Restriction map for
pF1KB3373
(Circular) (Six-base) MAPSORT of: pF1KB3373.seq Check: 241 from: 1 to: 4970
pF1KB3373 4970 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 171 1217 2008 171
Size: 1046 791 3133
Fragments arranged by size:
3133 1046 791
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1615 1718 1907 2795 3227 3580 4242 262
Size: 1353 103 189 888 432 353 662 990
Fragments arranged by size:
1353 990 888 662 432 353 189 103
AflII C'TTAA_G
Cuts at: 1029 1938 1029
Size: 909 4061
AflIII A'CryG_T
Cuts at: 1946 2322 4122 4403 1946
Size: 376 1800 281 2513
Fragments arranged by size:
2513 1800 376 281
AhdI GACnn_n'nnGTC
Cuts at: 1640 1640
Size: 4970
AleI CACnn'nnGTG
Cuts at: 1317 1317
Size: 4970
AlwNI CAG_nnn'CTG
Cuts at: 1335 1509 2275 3713 1335
Size: 174 766 1438 2592
Fragments arranged by size:
2592 1438 766 174
ApaLI G'TGCA_C
Cuts at: 3808 3808
Size: 4970
ApoI r'AATT_y
Cuts at: 247 738 844 877 1325 1795 1974 4323 4579
Size: 491 106 33 448 470 179 2349 256
Cuts at: 4579 247
Size: 638
Fragments arranged by size:
2349 638 491 470 448 256 179 106
33
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 1157 1877 1990 1157
Size: 720 113 4137
Fragments arranged by size:
4137 720 113
AvrII C'CTAG_G
Cuts at: 811 811
Size: 4970
BamHI G'GATC_C
Cuts at: 711 1386 1995 711
Size: 675 609 3686
Fragments arranged by size:
3686 675 609
BanI G'GyrC_C
Cuts at: 1986 2400 2648 2683 1986
Size: 414 248 35 4273
Fragments arranged by size:
4273 414 248 35
BanII G_rGCy'C
Cuts at: 819 1164 1696 1984 3014 819
Size: 345 532 288 1030 2775
Fragments arranged by size:
2775 1030 532 345 288
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3924 4970 2868
Size: 1056 1046 2868
Fragments arranged by size:
2868 1056 1046
BclI T'GATC_A
Cuts at: 114 1020 2490 114
Size: 906 1470 2594
Fragments arranged by size:
2594 1470 906
BglII A'GATC_T
Cuts at: 306 313 2485 4522 306
Size: 7 2172 2037 754
Fragments arranged by size:
2172 2037 754 7
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 1678 2595 2688 3812 1678
Size: 917 93 1124 2836
Fragments arranged by size:
2836 1124 917 93
BmrI ACTGGGnnnn_n'
Cuts at: 2182 2263 2598 4742 2182
Size: 81 335 2144 2410
Fragments arranged by size:
2410 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1903 3337 1903
Size: 1434 3536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1194 1531 1761 1194
Size: 337 230 4403
Fragments arranged by size:
4403 337 230
Bpu10I CC'TnA_GC
Cuts at: 747 747
Size: 4970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 436 1336 2146 3025 3739 4037 377
Size: 59 900 810 879 714 298 1310
Fragments arranged by size:
1310 900 879 810 714 298 59
BsaI GGTCTCn'nnnn_
Cuts at: 1001 1285 1911 4674 1001
Size: 284 626 2763 1297
Fragments arranged by size:
2763 1297 626 284
BsaAI yAC'GTr
Cuts at: 2323 2323
Size: 4970
BsaBI GATnn'nnATC
Cuts at: 6 836 2484 4521 6
Size: 830 1648 2037 455
Fragments arranged by size:
2037 1648 830 455
BsaHI Gr'CG_yC
Cuts at: 2401 2649 3351 4847 2401
Size: 248 702 1496 2524
Fragments arranged by size:
2524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2159 2680 3358 3769 3916 4190 2159
Size: 521 678 411 147 274 2939
Fragments arranged by size:
2939 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 275 590 275
Size: 315 4655
BseYI C'CCAG_C
Cuts at: 1144 2395 3153 3818 4226 1144
Size: 1251 758 665 408 1888
Fragments arranged by size:
1888 1251 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2512 2558 3788 104
Size: 2408 46 1230 1286
Fragments arranged by size:
2408 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1164 1984 2762 2952 3812 1164
Size: 820 778 190 860 2322
Fragments arranged by size:
2322 860 820 778 190
BsmI GAATG_Cn'
Cuts at: 812 812
Size: 4970
Bsp1286I G_dGCh'C
Cuts at: 819 1164 1678 1696 1984 2595 2688 2762 2952
Size: 345 514 18 288 611 93 74 190
Cuts at: 2952 3014 3812 819
Size: 62 798 1977
Fragments arranged by size:
1977 798 611 514 345 288 190 93
74 62 18
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2020 2234 2536 2917 2020
Size: 214 302 381 4073
Fragments arranged by size:
4073 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3262 3316 4800 4965 50
Size: 3212 54 1484 165 55
Fragments arranged by size:
3212 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 502 1811 2882 502
Size: 1309 1071 2590
Fragments arranged by size:
2590 1309 1071
BsrFI r'CCGG_y
Cuts at: 2968 3149 2968
Size: 181 4789
BssSI C'ACGA_G
Cuts at: 3241 3949 3241
Size: 708 4262
BstAPI GCAn_nnn'nTGC
Cuts at: 1810 2347 1810
Size: 537 4433
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 1416 1416
Size: 4970
BstYI r'GATC_y
Cuts at: 1 306 313 348 711 1386 1995 2462 2485
Size: 305 7 35 363 675 609 467 23
Cuts at: 2485 2820 3066 3372 3384 3470 4522 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 675 609 467 449 363 335 306
305 246 86 35 23 12 7
BtgI C'CryG_G
Cuts at: 107 107
Size: 4970
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 2213 2213
Size: 4970
DraI TTT'AAA
Cuts at: 1821 1969 1821
Size: 148 4822
DrdI GACnn_nn'nnGTC
Cuts at: 2676 4020 2676
Size: 1344 3626
EaeI y'GGCC_r
Cuts at: 1194 2555 2729 3120 3147 4492 1194
Size: 1361 174 391 27 1345 1672
Fragments arranged by size:
1672 1361 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2555 2555
Size: 4970
EarI CTCTTCn'nnn_
Cuts at: 439 1239 2993 3203 439
Size: 800 1754 210 2206
Fragments arranged by size:
2206 1754 800 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3178 3920 4066 4780 3178
Size: 742 146 714 3368
Fragments arranged by size:
3368 742 714 146
EcoICRI GAG'CTC
Cuts at: 1162 1982 1162
Size: 820 4150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1194 1531 1615 1718 1761 1907 2795 3227
Size: 932 337 84 103 43 146 888 432
Cuts at: 3227 3580 4242 262
Size: 353 662 990
Fragments arranged by size:
990 932 888 662 432 353 337 146
103 84 43
EcoNI CCTnn'n_nnAGG
Cuts at: 160 605 1209 160
Size: 445 604 3921
Fragments arranged by size:
3921 604 445
EcoO109I rG'GnC_Cy
Cuts at: 574 974 2109 574
Size: 400 1135 3435
Fragments arranged by size:
3435 1135 400
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
EcoRV GAT'ATC
Cuts at: 1557 1557
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4970
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 1469 2404 2652 3882 4653 1469
Size: 935 248 1230 771 1786
Fragments arranged by size:
1786 1230 935 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 703 735 996 1028 1626 1658 2812
Size: 32 373 32 261 32 598 32 1154
Cuts at: 2812 2842 2844 2874 298
Size: 30 2 30 2394
Fragments arranged by size:
2394 1154 598 373 261 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 172 2009 172
Size: 1837 3133
HindIII A'AGCT_T
Cuts at: 122 122
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1189 3729 3913 1189
Size: 2540 184 2246
Fragments arranged by size:
2540 2246 184
MscI TGG'CCA
Cuts at: 1196 2731 1196
Size: 1535 3435
MslI CAynn'nnrTG
Cuts at: 1317 1414 1951 2959 3086 1317
Size: 97 537 1008 127 3201
Fragments arranged by size:
3201 1008 537 127 97
MspA1I CmG'CkG
Cuts at: 1927 2081 2395 2755 3539 3784 1927
Size: 154 314 360 784 245 3113
Fragments arranged by size:
3113 784 360 314 245 154
NaeI GCC'GGC
Cuts at: 3151 3151
Size: 4970
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 107 107
Size: 4970
NdeI CA'TA_TG
Cuts at: 440 1461 440
Size: 1021 3949
NgoMIV G'CCGG_C
Cuts at: 3149 3149
Size: 4970
NspI r_CATG'y
Cuts at: 1754 1950 2023 2523 4126 1754
Size: 196 73 500 1603 2598
Fragments arranged by size:
2598 1603 500 196 73
PasI CC'CwG_GG
Cuts at: 978 978
Size: 4970
PciI A'CATG_T
Cuts at: 1946 4122 1946
Size: 2176 2794
PflMI CCAn_nnn'nTGG
Cuts at: 708 708
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4938
PpuMI rG'GwC_Cy
Cuts at: 574 974 574
Size: 400 4570
PspXI vC'TCGA_Gb
Cuts at: 1157 1157
Size: 4970
PstI C_TGCA'G
Cuts at: 2017 2702 2017
Size: 685 4285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 1927 2395 2755 1927
Size: 468 360 4142
Fragments arranged by size:
4142 468 360
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 1164 1984 1164
Size: 820 4150
SalI G'TCGA_C
Cuts at: 170 2007 170
Size: 1837 3133
SapI GCTCTTCn'nnn_
Cuts at: 439 2993 3203 439
Size: 2554 210 2206
Fragments arranged by size:
2554 2206 210
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 4487 4487
Size: 4970
SexAI A'CCwGG_T
Cuts at: 632 632
Size: 4970
SfcI C'TryA_G
Cuts at: 33 2013 2698 3666 3857 33
Size: 1980 685 968 191 1146
Fragments arranged by size:
1980 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 392 451 1029 1157 1315 1877 1938 2125 3040
Size: 59 578 128 158 562 61 187 915
Cuts at: 3040 3754 4016 392
Size: 714 262 1346
Fragments arranged by size:
1346 915 714 578 562 262 187 158
128 61 59
SpeI A'CTAG_T
Cuts at: 67 580 658 1036 67
Size: 513 78 378 4001
Fragments arranged by size:
4001 513 378 78
SphI G_CATG'C
Cuts at: 2023 2523 2023
Size: 500 4470
StyI C'CwwG_G
Cuts at: 107 811 1197 1786 2104 2458 107
Size: 704 386 589 318 354 2619
Fragments arranged by size:
2619 704 589 386 354 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1158 2553 2686 3145 3354 1158
Size: 1395 133 459 209 2774
Fragments arranged by size:
2774 1395 459 209 133
TatI w'GTAC_w
Cuts at: 478 1753 4485 478
Size: 1275 2732 963
Fragments arranged by size:
2732 1275 963
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4487 4519 4487
Size: 32 4938
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 1176 1204 2465 369
Size: 807 28 1261 2874
Fragments arranged by size:
2874 1261 807 28
XhoI C'TCGA_G
Cuts at: 1157 1877 1157
Size: 720 4250
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI