Restriction map for
pF1KB3347
(Circular) (Six-base) MAPSORT of: pF1KB3347.seq Check: 7323 from: 1 to: 5324
pF1KB3347 5324 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1352 1352
Size: 5324
AccI GT'mk_AC
Cuts at: 2362 2362
Size: 5324
Acc65I G'GTAC_C
Cuts at: 2340 2340
Size: 5324
AclI AA'CG_TT
Cuts at: 226 5164 226
Size: 4938 386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 953 1936 2078 3149 3581 3934 4596 349
Size: 604 983 142 1071 432 353 662 1077
Fragments arranged by size:
1077 1071 983 662 604 432 353 142
AflII C'TTAA_G
Cuts at: 135 135
Size: 5324
AflIII A'CryG_T
Cuts at: 2676 4476 4757 2676
Size: 1800 281 3243
Fragments arranged by size:
3243 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 199 777 834 1173 1332 1497 2081 2629 4067
Size: 578 57 339 159 165 584 548 1438
Cuts at: 4067 199
Size: 1456
Fragments arranged by size:
1456 1438 584 578 548 339 165 159
57
ApaI G_GGCC'C
Cuts at: 972 972
Size: 5324
ApaLI G'TGCA_C
Cuts at: 4162 4162
Size: 5324
ApoI r'AATT_y
Cuts at: 2328 4677 4933 2328
Size: 2349 256 2719
Fragments arranged by size:
2719 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5324
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5324
AvaI C'yCGr_G
Cuts at: 608 707 1040 2344 608
Size: 99 333 1304 3588
Fragments arranged by size:
3588 1304 333 99
BamHI G'GATC_C
Cuts at: 2349 2349
Size: 5324
BanI G'GyrC_C
Cuts at: 2340 2754 3002 3037 2340
Size: 414 248 35 4627
Fragments arranged by size:
4627 414 248 35
BanII G_rGCy'C
Cuts at: 434 972 1041 1047 1594 1757 2100 2338 3368
Size: 538 69 6 547 163 343 238 1030
Cuts at: 3368 434
Size: 2390
Fragments arranged by size:
2390 1030 547 538 343 238 163 69
6
BbeI G_GCGC'C
Cuts at: 2758 3006 2758
Size: 248 5076
BbsI GAAGACnn'nnnn_
Cuts at: 941 1245 1924 941
Size: 304 679 4341
Fragments arranged by size:
4341 679 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5290
BciVI GTATCCnnnnn_n'
Cuts at: 1659 3222 4278 5324 1659
Size: 1563 1056 1046 1659
Fragments arranged by size:
1659 1563 1056 1046
BclI T'GATC_A
Cuts at: 1049 2844 1049
Size: 1795 3529
BfrBI ATG'CAT
Cuts at: 1952 1952
Size: 5324
BglII A'GATC_T
Cuts at: 2839 4876 2839
Size: 2037 3287
BlpI GC'TnA_GC
Cuts at: 2436 2436
Size: 5324
Bme1580I G_kGCm'C
Cuts at: 972 2949 3042 4166 972
Size: 1977 93 1124 2130
Fragments arranged by size:
2130 1977 1124 93
BmgBI CAC'GTC
Cuts at: 1538 2026 1538
Size: 488 4836
BmrI ACTGGGnnnn_n'
Cuts at: 428 641 2536 2617 2952 5096 428
Size: 213 1895 81 335 2144 656
Fragments arranged by size:
2144 1895 656 335 213 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3691 3691
Size: 5324
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1249 856
Size: 393 4931
Bpu10I CC'TnA_GC
Cuts at: 212 1705 212
Size: 1493 3831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 3379 4093 4391 2500
Size: 879 714 298 3433
Fragments arranged by size:
3433 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 457 1103 1150 5028 457
Size: 646 47 3878 753
Fragments arranged by size:
3878 753 646 47
BsaAI yAC'GTr
Cuts at: 2677 2677
Size: 5324
BsaBI GATnn'nnATC
Cuts at: 6 569 2213 2838 4875 6
Size: 563 1644 625 2037 455
Fragments arranged by size:
2037 1644 625 563 455
BsaHI Gr'CG_yC
Cuts at: 1349 2755 3003 3705 5201 1349
Size: 1406 248 702 1496 1472
Fragments arranged by size:
1496 1472 1406 702 248
BsaWI w'CCGG_w
Cuts at: 1134 2513 3034 3712 4123 4270 4544 1134
Size: 1379 521 678 411 147 274 1914
Fragments arranged by size:
1914 1379 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 168 823 1437 168
Size: 655 614 4055
Fragments arranged by size:
4055 655 614
BseYI C'CCAG_C
Cuts at: 389 749 1738 1900 2749 3507 4172 4580 389
Size: 360 989 162 849 758 665 408 1133
Fragments arranged by size:
1133 989 849 758 665 408 360 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1408 1483 304
Size: 1104 75 4145
Fragments arranged by size:
4145 1104 75
BsiEI CG_ry'CG
Cuts at: 104 2866 2912 4142 104
Size: 2762 46 1230 1286
Fragments arranged by size:
2762 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 590 1041 1605 2338 3116 3306 4166 590
Size: 451 564 733 778 190 860 1748
Fragments arranged by size:
1748 860 778 733 564 451 190
Bsp1286I G_dGCh'C
Cuts at: 434 590 972 1041 1047 1594 1605 1757 2100
Size: 156 382 69 6 547 11 152 343
Cuts at: 2100 2338 2949 3042 3116 3306 3368 4166 434
Size: 238 611 93 74 190 62 798 1592
Fragments arranged by size:
1592 798 611 547 382 343 238 190
156 152 93 74 69 62 11 6
BspHI T'CATG_A
Cuts at: 2313 3756 2313
Size: 1443 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2374 2588 2890 3271 2374
Size: 214 302 381 4427
Fragments arranged by size:
4427 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3616 3670 5154 5319 50
Size: 3566 54 1484 165 55
Fragments arranged by size:
3566 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 672 1389 1838 3236 672
Size: 717 449 1398 2760
Fragments arranged by size:
2760 1398 717 449
BsrFI r'CCGG_y
Cuts at: 140 3322 3503 140
Size: 3182 181 1961
Fragments arranged by size:
3182 1961 181
BsrGI T'GTAC_A
Cuts at: 2090 2090
Size: 5324
BssHII G'CGCG_C
Cuts at: 603 603
Size: 5324
BssSI C'ACGA_G
Cuts at: 1671 3595 4303 1671
Size: 1924 708 2692
Fragments arranged by size:
2692 1924 708
BstAPI GCAn_nnn'nTGC
Cuts at: 208 2701 208
Size: 2493 2831
BstBI TT'CG_AA
Cuts at: 3685 3685
Size: 5324
BstEII G'GTnAC_C
Cuts at: 922 2046 922
Size: 1124 4200
BstYI r'GATC_y
Cuts at: 1 953 1268 2349 2816 2839 3174 3420 3726
Size: 952 315 1081 467 23 335 246 306
Cuts at: 3726 3738 3824 4876 1
Size: 12 86 1052 449
Fragments arranged by size:
1081 1052 952 467 449 335 315 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1247 1247
Size: 5324
BtgI C'CryG_G
Cuts at: 1113 1523 1113
Size: 410 4914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3274 3455 3618 3274
Size: 181 163 4980
Fragments arranged by size:
4980 181 163
BtsI GCAGTG_nn'
Cuts at: 1178 1342 1420 2567 1178
Size: 164 78 1147 3935
Fragments arranged by size:
3935 1147 164 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 402 437 1699 1734 402
Size: 35 1262 35 3992
Fragments arranged by size:
3992 1262 35 35
DraI TTT'AAA
Cuts at: 2323 2323
Size: 5324
DrdI GACnn_nn'nnGTC
Cuts at: 1207 1689 3030 4374 1207
Size: 482 1341 1344 2157
Fragments arranged by size:
2157 1344 1341 482
EaeI y'GGCC_r
Cuts at: 1223 1394 1482 2909 3083 3474 3501 4846 1223
Size: 171 88 1427 174 391 27 1345 1701
Fragments arranged by size:
1701 1427 1345 391 174 171 88 27
EagI C'GGCC_G
Cuts at: 2909 2909
Size: 5324
EarI CTCTTCn'nnn_
Cuts at: 153 325 507 2054 3347 3557 153
Size: 172 182 1547 1293 210 1920
Fragments arranged by size:
1920 1547 1293 210 182 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1537 3532 4274 4420 5134 1537
Size: 1995 742 146 714 1727
Fragments arranged by size:
1995 1727 742 714 146
EcoICRI GAG'CTC
Cuts at: 1039 2336 1039
Size: 1297 4027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 856 953 1249 1936 2078 3149 3581 3934
Size: 507 97 296 687 142 1071 432 353
Cuts at: 3934 4596 349
Size: 662 1077
Fragments arranged by size:
1077 1071 687 662 507 432 353 296
142 97
EcoNI CCTnn'n_nnAGG
Cuts at: 368 368
Size: 5324
EcoO109I rG'GnC_Cy
Cuts at: 968 2463 968
Size: 1495 3829
EcoRI G'AATT_C
Cuts at: 2328 2328
Size: 5324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1941 2326 3343 1941
Size: 385 1017 3922
Fragments arranged by size:
3922 1017 385
FspI TGC'GCA
Cuts at: 3105 3105
Size: 5324
HaeII r_GCGC'y
Cuts at: 816 2758 3006 4236 5007 816
Size: 1942 248 1230 771 1133
Fragments arranged by size:
1942 1230 1133 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1034 1066 1274 1306 1563 1595 1621 1653 1680
Size: 32 208 32 257 32 26 32 27
Cuts at: 1680 1712 3166 3196 3198 3228 1034
Size: 32 1454 30 2 30 3130
Fragments arranged by size:
3130 1454 257 208 32 32 32 32
32 30 30 27 26 2
HincII GTy'rAC
Cuts at: 165 2363 165
Size: 2198 3126
HindIII A'AGCT_T
Cuts at: 742 742
Size: 5324
KasI G'GCGC_C
Cuts at: 2754 3002 2754
Size: 248 5076
KpnI G_GTAC'C
Cuts at: 2344 2344
Size: 5324
MluI A'CGCG_T
Cuts at: 4757 4757
Size: 5324
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 963 1955 4083 4267 963
Size: 992 2128 184 2020
Fragments arranged by size:
2128 2020 992 184
MscI TGG'CCA
Cuts at: 1396 3085 1396
Size: 1689 3635
MslI CAynn'nnrTG
Cuts at: 172 293 1183 2008 3313 3440 172
Size: 121 890 825 1305 127 2056
Fragments arranged by size:
2056 1305 890 825 127 121
MspA1I CmG'CkG
Cuts at: 393 449 777 822 834 855 864 1477 2435
Size: 56 328 45 12 21 9 613 958
Cuts at: 2435 2749 3109 3893 4138 393
Size: 314 360 784 245 1579
Fragments arranged by size:
1579 958 784 613 360 328 314 245
56 45 21 12 9
NaeI GCC'GGC
Cuts at: 142 3505 142
Size: 3363 1961
NarI GG'CG_CC
Cuts at: 2755 3003 2755
Size: 248 5076
NcoI C'CATG_G
Cuts at: 1113 1113
Size: 5324
NgoMIV G'CCGG_C
Cuts at: 140 3503 140
Size: 3363 1961
NsiI A_TGCA'T
Cuts at: 1954 1954
Size: 5324
NspI r_CATG'y
Cuts at: 292 846 945 2109 2377 2877 4480 292
Size: 554 99 1164 268 500 1603 1136
Fragments arranged by size:
1603 1164 1136 554 500 268 99
PciI A'CATG_T
Cuts at: 4476 4476
Size: 5324
PflMI CCAn_nnn'nTGG
Cuts at: 777 777
Size: 5324
PfoI T'CCnGG_A
Cuts at: 369 956 2150 2221 369
Size: 587 1194 71 3472
Fragments arranged by size:
3472 1194 587 71
PmeI GTTT'AAAC
Cuts at: 2323 2323
Size: 5324
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 5292
PspOMI G'GGCC_C
Cuts at: 968 968
Size: 5324
PspXI vC'TCGA_Gb
Cuts at: 1040 1040
Size: 5324
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2095 2127 2095
Size: 32 5292
PstI C_TGCA'G
Cuts at: 869 1337 2371 3056 869
Size: 468 1034 685 3137
Fragments arranged by size:
3137 1034 685 468
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5324
PvuII CAG'CTG
Cuts at: 393 777 822 834 855 864 2749 3109 393
Size: 384 45 12 21 9 1885 360 2608
Fragments arranged by size:
2608 1885 384 360 45 21 12 9
RsrII CG'GwC_CG
Cuts at: 3519 3519
Size: 5324
SacI G_AGCT'C
Cuts at: 1041 2338 1041
Size: 1297 4027
SalI G'TCGA_C
Cuts at: 2361 2361
Size: 5324
SapI GCTCTTCn'nnn_
Cuts at: 2054 3347 3557 2054
Size: 1293 210 3821
Fragments arranged by size:
3821 1293 210
SbfI CC_TGCA'GG
Cuts at: 2371 2371
Size: 5324
ScaI AGT'ACT
Cuts at: 2055 4841 2055
Size: 2786 2538
SfcI C'TryA_G
Cuts at: 33 865 1333 2367 3052 4020 4211 33
Size: 832 468 1034 685 968 191 1146
Fragments arranged by size:
1146 1034 968 832 685 468 191
SfoI GGC'GCC
Cuts at: 2756 3004 2756
Size: 248 5076
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5324
SmaI CCC'GGG
Cuts at: 610 2346 610
Size: 1736 3588
SmlI C'TyrA_G
Cuts at: 135 1040 2479 3394 4108 4370 135
Size: 905 1439 915 714 262 1089
Fragments arranged by size:
1439 1089 915 905 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5324
SphI G_CATG'C
Cuts at: 292 945 2377 2877 292
Size: 653 1432 500 2739
Fragments arranged by size:
2739 1432 653 500
StuI AGG'CCT
Cuts at: 546 546
Size: 5324
StyI C'CwwG_G
Cuts at: 249 1113 2458 2812 249
Size: 864 1345 354 2761
Fragments arranged by size:
2761 1345 864 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1049 1866 2907 3040 3499 3708 1049
Size: 817 1041 133 459 209 2665
Fragments arranged by size:
2665 1041 817 459 209 133
TatI w'GTAC_w
Cuts at: 312 460 474 2053 2090 4839 312
Size: 148 14 1579 37 2749 797
Fragments arranged by size:
2749 1579 797 148 37 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4841 4873 4841
Size: 32 5292
Tth111I GACn'n_nGTC
Cuts at: 979 3121 979
Size: 2142 3182
XbaI T'CTAG_A
Cuts at: 2355 2355
Size: 5324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2819 2819
Size: 5324
XhoI C'TCGA_G
Cuts at: 1040 1040
Size: 5324
XmaI C'CCGG_G
Cuts at: 608 2344 608
Size: 1736 3588
ZraI GAC'GTC
Cuts at: 1350 1350
Size: 5324
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BglI BmtI BsaXI BsiWI BsmI
BsmBI BspEI BstXI BstZ17I ClaI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PmlI PpuMI PshAI PsiI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI