Restriction map for pF1KB3307
 (Circular) (Six-base) MAPSORT of: pF1KB3307.seq  Check: 1177  from: 1  to: 5267



pF1KB3307  5267 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AccI GT'mk_AC

Cuts at:    184    2305     184 
   Size:       2121    3146
 
Acc65I G'GTAC_C

Cuts at:   2283    2283 
   Size:       5267
 
AclI AA'CG_TT

Cuts at:   5107    5107 
   Size:       5267
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    889     890    1684    3092    3524    3877    4539     889 
   Size:          1     794    1408     432     353     662    1617

  Fragments arranged by size: 

                1617    1408     794     662     432     353       1
 
AflIII A'CryG_T

Cuts at:   1229    2619    4419    4700    1229 
   Size:       1390    1800     281    1796

  Fragments arranged by size: 

                1800    1796    1390     281
 
AleI CACnn'nnGTG

Cuts at:   1764    1764 
   Size:       5267
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1850    1929    1850 
   Size:         79    5188
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    874     906     874 
   Size:         32    5235
 
AlwNI CAG_nnn'CTG

Cuts at:    388     397     451     535     679    1255    1373    1662    2013 
   Size:          9      54      84     144     576     118     289     351

Cuts at:   2013    2053    2214    2572    4010     388 
   Size:         40     161     358    1438    1645

  Fragments arranged by size: 

                1645    1438     576     358     351     289     161     144
                 118      84      54      40       9
 
ApaI G_GGCC'C

Cuts at:    835     915    2088     835 
   Size:         80    1173    4014

  Fragments arranged by size: 

                4014    1173      80
 
ApaLI G'TGCA_C

Cuts at:   1732    4105    1732 
   Size:       2373    2894
 
ApoI r'AATT_y

Cuts at:   1669    2255    2271    4620    4876    1669 
   Size:        586      16    2349     256    2060

  Fragments arranged by size: 

                2349    2060     586     256      16
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5267
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5267
 
AvaI C'yCGr_G

Cuts at:   2162    2287    2162 
   Size:        125    5142
 
AvrII C'CTAG_G

Cuts at:    546     546 
   Size:       5267
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    377     410     377 
   Size:         33    5234
 
BamHI G'GATC_C

Cuts at:   2292    2292 
   Size:       5267
 
BanI G'GyrC_C

Cuts at:   1705    1834    2283    2697    2945    2980    1705 
   Size:        129     449     414     248      35    3992

  Fragments arranged by size: 

                3992     449     414     248     129      35
 
BanII G_rGCy'C

Cuts at:    634     835     915    1040    1063    1413    2088    2281    3311 
   Size:        201      80     125      23     350     675     193    1030

Cuts at:   3311     634 
   Size:       2590

  Fragments arranged by size: 

                2590    1030     675     350     201     193     125      80
                  23
 
BbeI G_GCGC'C

Cuts at:   2701    2949    2701 
   Size:        248    5019
 
BbsI GAAGACnn'nnnn_

Cuts at:    896    1232     896 
   Size:        336    4931
 
BbvCI CC'TCA_GC

Cuts at:    967    2065     967 
   Size:       1098    4169
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    164     198    1445    1479     164 
   Size:         34    1247      34    3952

  Fragments arranged by size: 

                3952    1247      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3165    4221    5267    3165 
   Size:       1056    1046    3165

  Fragments arranged by size: 

                3165    1056    1046
 
BclI T'GATC_A

Cuts at:   2787    2787 
   Size:       5267
 
BglI GCCn_nnn'nGGC

Cuts at:   1935    1999    1935 
   Size:         64    5203
 
BglII A'GATC_T

Cuts at:   2782    4819    2782 
   Size:       2037    3230
 
BlpI GC'TnA_GC

Cuts at:   2379    2379 
   Size:       5267
 
Bme1580I G_kGCm'C

Cuts at:    835     915    1208    1736    1839    2088    2892    2985    4109 
   Size:         80     293     528     103     249     804      93    1124

Cuts at:   4109     835 
   Size:       1993

  Fragments arranged by size: 

                1993    1124     804     528     293     249     103      93
                  80
 
BmgBI CAC'GTC

Cuts at:   1731    1731 
   Size:       5267
 
BmrI ACTGGGnnnn_n'

Cuts at:   1218    2479    2560    2895    5039    1218 
   Size:       1261      81     335    2144    1446

  Fragments arranged by size: 

                2144    1446    1261     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1888    3634    1888 
   Size:       1746    3521
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    326     448     492    1718    1798    1861     326 
   Size:        122      44    1226      80      63    3732

  Fragments arranged by size: 

                3732    1226     122      80      63      44
 
Bpu10I CC'TnA_GC

Cuts at:    242     967    2065     242 
   Size:        725    1098    3444

  Fragments arranged by size: 

                3444    1098     725
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    386    1303    2443    3322    4036    4334     386 
   Size:        917    1140     879     714     298    1319

  Fragments arranged by size: 

                1319    1140     917     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4971    4971 
   Size:       5267
 
BsaAI yAC'GTr

Cuts at:   2620    2620 
   Size:       5267
 
BsaBI GATnn'nnATC

Cuts at:      6    2781    4818       6 
   Size:       2775    2037     455

  Fragments arranged by size: 

                2775    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2698    2946    3648    5144    2698 
   Size:        248     702    1496    2821

  Fragments arranged by size: 

                2821    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    692    2456    2977    3655    4066    4213    4487     692 
   Size:       1764     521     678     411     147     274    1472

  Fragments arranged by size: 

                1764    1472     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    241     781     241 
   Size:        540    4727
 
BseYI C'CCAG_C

Cuts at:   1267    2087    2692    3450    4115    4523    1267 
   Size:        820     605     758     665     408    2011

  Fragments arranged by size: 

                2011     820     758     665     605     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1261    1322    2227    1261 
   Size:         61     905    4301

  Fragments arranged by size: 

                4301     905      61
 
BsiEI CG_ry'CG

Cuts at:    104    2809    2855    4085     104 
   Size:       2705      46    1230    1286

  Fragments arranged by size: 

                2705    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1040    1736    1874    2281    3059    3249    4109    1040 
   Size:        696     138     407     778     190     860    2198

  Fragments arranged by size: 

                2198     860     778     696     407     190     138
 
Bsp1286I G_dGCh'C

Cuts at:    634     835     915    1040    1063    1208    1413    1736    1839 
   Size:        201      80     125      23     145     205     323     103

Cuts at:   1839    1874    2088    2281    2892    2985    3059    3249    3311 
   Size:         35     214     193     611      93      74     190      62

Cuts at:   3311    4109     634 
   Size:        798    1792

  Fragments arranged by size: 

                1792     798     611     323     214     205     201     193
                 190     145     125     103      93      80      74      62
                  35      23
 
BspEI T'CCGG_A

Cuts at:    692     692 
   Size:       5267
 
BspHI T'CATG_A

Cuts at:   3699    3699 
   Size:       5267
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2317    2531    2833    3214    2317 
   Size:        214     302     381    4370

  Fragments arranged by size: 

                4370     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     210     432     654    3559    3613    5097    5262      50 
   Size:        160     222     222    2905      54    1484     165      55

  Fragments arranged by size: 

                2905    1484     222     222     165     160      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   3179    3179 
   Size:       5267
 
BsrFI r'CCGG_y

Cuts at:   1013    1361    1754    3265    3446    1013 
   Size:        348     393    1511     181    2834

  Fragments arranged by size: 

                2834    1511     393     348     181
 
BsrGI T'GTAC_A

Cuts at:   1495    1495 
   Size:       5267
 
BssSI C'ACGA_G

Cuts at:   1085    2232    3538    4246    1085 
   Size:       1147    1306     708    2106

  Fragments arranged by size: 

                2106    1306    1147     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:    388     397    2013    2644     388 
   Size:          9    1616     631    3011

  Fragments arranged by size: 

                3011    1616     631       9
 
BstBI TT'CG_AA

Cuts at:   3628    3628 
   Size:       5267
 
BstYI r'GATC_y

Cuts at:      1    1369    1520    2292    2759    2782    3117    3363    3669 
   Size:       1368     151     772     467      23     335     246     306

Cuts at:   3669    3681    3767    4819       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1368    1052     772     467     449     335     306     246
                 151      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   2198    2198 
   Size:       5267
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       5267
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3217    3398    3561    3217 
   Size:        181     163    4923

  Fragments arranged by size: 

                4923     181     163
 
BtsI GCAGTG_nn'

Cuts at:    398     500     922    1341    1874    2510     398 
   Size:        102     422     419     533     636    3155

  Fragments arranged by size: 

                3155     636     533     422     419     102
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    182     217     182 
   Size:         35    5232
 
DraI TTT'AAA

Cuts at:   2266    2266 
   Size:       5267
 
DrdI GACnn_nn'nnGTC

Cuts at:   2973    4317    2973 
   Size:       1344    3923
 
EaeI y'GGCC_r

Cuts at:   1597    1756    1927    2121    2852    3026    3417    3444    4789 
   Size:        159     171     194     731     174     391      27    1345

Cuts at:   4789    1597 
   Size:       2075

  Fragments arranged by size: 

                2075    1345     731     391     194     174     171     159
                  27
 
EagI C'GGCC_G

Cuts at:   2852    2852 
   Size:       5267
 
EarI CTCTTCn'nnn_

Cuts at:   1045    3290    3500    1045 
   Size:       2245     210    2812

  Fragments arranged by size: 

                2812    2245     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1567    2068    3475    4217    4363    5077    1567 
   Size:        501    1407     742     146     714    1757

  Fragments arranged by size: 

                1757    1407     742     714     501     146
 
EcoICRI GAG'CTC

Cuts at:   1038    2279    1038 
   Size:       1241    4026
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    326     448     492     889     890    1684    1718    1798    1861 
   Size:        122      44     397       1     794      34      80      63

Cuts at:   1861    3092    3524    3877    4539     326 
   Size:       1231     432     353     662    1054

  Fragments arranged by size: 

                1231    1054     794     662     432     397     353     122
                  80      63      44      34       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:    223     610     693     826     897    1654     223 
   Size:        387      83     133      71     757    3836

  Fragments arranged by size: 

                3836     757     387     133      83      71
 
EcoO109I rG'GnC_Cy

Cuts at:    831     832     911     912    1617    1647    1659    1686    2238 
   Size:          1      79       1     705      30      12      27     552

Cuts at:   2238    2406     831 
   Size:        168    3692

  Fragments arranged by size: 

                3692     705     552     168      79      30      27      12
                   1       1
 
EcoRI G'AATT_C

Cuts at:   2271    2271 
   Size:       5267
 
EcoRV GAT'ATC

Cuts at:    296     296 
   Size:       5267
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    774    3286     774 
   Size:       2512    2755
 
FseI GG_CCGG'CC

Cuts at:   1758    1758 
   Size:       5267
 
FspI TGC'GCA

Cuts at:   3048    3048 
   Size:       5267
 
HaeII r_GCGC'y

Cuts at:   2701    2949    4179    4950    2701 
   Size:        248    1230     771    3018

  Fragments arranged by size: 

                3018    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1678    1710    1796    1828    2035    2048    2067    2080    3109 
   Size:         32      86      32     207      13      19      13    1029

Cuts at:   3109    3139    3141    3171    1678 
   Size:         30       2      30    3774

  Fragments arranged by size: 

                3774    1029     207      86      32      32      30      30
                  19      13      13       2
 
HincII GTy'rAC

Cuts at:    185     819    2306     185 
   Size:        634    1487    3146

  Fragments arranged by size: 

                3146    1487     634
 
HindIII A'AGCT_T

Cuts at:    751     751 
   Size:       5267
 
KasI G'GCGC_C

Cuts at:   2697    2945    2697 
   Size:        248    5019
 
KpnI G_GTAC'C

Cuts at:   2287    2287 
   Size:       5267
 
MluI A'CGCG_T

Cuts at:   4700    4700 
   Size:       5267
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    808    1815    4026    4210     808 
   Size:       1007    2211     184    1865

  Fragments arranged by size: 

                2211    1865    1007     184
 
MscI TGG'CCA

Cuts at:   1929    2123    3028    1929 
   Size:        194     905    4168

  Fragments arranged by size: 

                4168     905     194
 
MslI CAynn'nnrTG

Cuts at:    894    1764    1766    1966    3256    3383     894 
   Size:        870       2     200    1290     127    2778

  Fragments arranged by size: 

                2778    1290     870     200     127       2
 
MspA1I CmG'CkG

Cuts at:    535     676    1509    2378    2692    3052    3836    4081     535 
   Size:        141     833     869     314     360     784     245    1721

  Fragments arranged by size: 

                1721     869     833     784     360     314     245     141
            
 
NaeI GCC'GGC

Cuts at:   1756    3448    1756 
   Size:       1692    3575
 
NarI GG'CG_CC

Cuts at:   2698    2946    2698 
   Size:        248    5019
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       5267
 
NgoMIV G'CCGG_C

Cuts at:   1754    3446    1754 
   Size:       1692    3575
 
NspI r_CATG'y

Cuts at:   1233    2320    2820    4423    1233 
   Size:       1087     500    1603    2077

  Fragments arranged by size: 

                2077    1603    1087     500
 
PasI CC'CwG_GG

Cuts at:   1063    1682    1749    1063 
   Size:        619      67    4581

  Fragments arranged by size: 

                4581     619      67
 
PciI A'CATG_T

Cuts at:   1229    4419    1229 
   Size:       3190    2077
 
PflMI CCAn_nnn'nTGG

Cuts at:    522    1373    1956     522 
   Size:        851     583    3833

  Fragments arranged by size: 

                3833     851     583
 
PfoI T'CCnGG_A

Cuts at:    303     303 
   Size:       5267
 
PmeI GTTT'AAAC

Cuts at:   2266    2266 
   Size:       5267
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1022    1054    3713    3745    1022 
   Size:         32    2659      32    2544

  Fragments arranged by size: 

                2659    2544      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   1617    1647    1686    2238    1617 
   Size:         30      39     552    4646

  Fragments arranged by size: 

                4646     552      39      30
 
PspOMI G'GGCC_C

Cuts at:    831     911    2084     831 
   Size:         80    1173    4014

  Fragments arranged by size: 

                4014    1173      80
 
PstI C_TGCA'G

Cuts at:    393     565     929    2025    2314    2999     393 
   Size:        172     364    1096     289     685    2661

  Fragments arranged by size: 

                2661    1096     685     364     289     172
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5267
 
PvuII CAG'CTG

Cuts at:    535     676    1509    2692    3052     535 
   Size:        141     833    1183     360    2750

  Fragments arranged by size: 

                2750    1183     833     360     141
 
RsrII CG'GwC_CG

Cuts at:   3462    3462 
   Size:       5267
 
SacI G_AGCT'C

Cuts at:   1040    2281    1040 
   Size:       1241    4026
 
SalI G'TCGA_C

Cuts at:    183    2304     183 
   Size:       2121    3146
 
SanDI GG'GwC_CC

Cuts at:   1617    2238    1617 
   Size:        621    4646
 
SapI GCTCTTCn'nnn_

Cuts at:   1045    3290    3500    1045 
   Size:       2245     210    2812

  Fragments arranged by size: 

                2812    2245     210
 
SbfI CC_TGCA'GG

Cuts at:   2314    2314 
   Size:       5267
 
ScaI AGT'ACT

Cuts at:   4784    4784 
   Size:       5267
 
SfcI C'TryA_G

Cuts at:     33     389     561     925    2021    2224    2310    2995    3963 
   Size:        356     172     364    1096     203      86     685     968

Cuts at:   3963    4154      33 
   Size:        191    1146

  Fragments arranged by size: 

                1146    1096     968     685     364     356     203     191
                 172      86
 
SfoI GGC'GCC

Cuts at:   2699    2947    2699 
   Size:        248    5019
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5267
 
SgrAI Cr'CCGG_yG

Cuts at:   1013    1013 
   Size:       5267
 
SmaI CCC'GGG

Cuts at:   2289    2289 
   Size:       5267
 
SmlI C'TyrA_G

Cuts at:    401    1318    2162    2422    3337    4051    4313     401 
   Size:        917     844     260     915     714     262    1355

  Fragments arranged by size: 

                1355     917     915     844     714     262     260
 
SpeI A'CTAG_T

Cuts at:     67    1110      67 
   Size:       1043    4224
 
SphI G_CATG'C

Cuts at:   2320    2820    2320 
   Size:        500    4767
 
StuI AGG'CCT

Cuts at:   1263    1263 
   Size:       5267
 
StyI C'CwwG_G

Cuts at:    107     144     437     546    2178    2401    2755     107 
   Size:         37     293     109    1632     223     354    2619

  Fragments arranged by size: 

                2619    1632     354     293     223     109      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    529     791    2850    2983    3442    3651     529 
   Size:        262    2059     133     459     209    2145

  Fragments arranged by size: 

                2145    2059     459     262     209     133
 
TatI w'GTAC_w

Cuts at:    574    1495    4782     574 
   Size:        921    3287    1059

  Fragments arranged by size: 

                3287    1059     921
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4784    4816    4784 
   Size:         32    5235
 
Tth111I GACn'n_nGTC

Cuts at:   3064    3064 
   Size:       5267
 
XbaI T'CTAG_A

Cuts at:   2298    2298 
   Size:       5267
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1018    2762    1018 
   Size:       1744    3523
 
XhoI C'TCGA_G

Cuts at:   2162    2162 
   Size:       5267
 
XmaI C'CCGG_G

Cuts at:   2287    2287 
   Size:       5267
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AscI    BfrBI
     BmtI    BsaXI    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI
  BstZ17I     ClaI   DraIII    FspAI     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI    PspXI
     PsrI    SacII    SexAI     SfiI    SnaBI     SrfI     SspI     SwaI
     XmnI     ZraI
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