(Circular) (Six-base) MAPSORT of: pF1KB3201.seq Check: 822 from: 1 to: 4151 pF1KB3201 4151 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 1189 1189 Size: 4151 Acc65I G'GTAC_C Cuts at: 1167 1167 Size: 4151 AclI AA'CG_TT Cuts at: 3991 3991 Size: 4151 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 873 1976 2408 3155 3423 873 Size: 1103 432 747 268 1601 Fragments arranged by size: 1601 1103 747 432 268 AflII C'TTAA_G Cuts at: 285 285 Size: 4151 AflIII A'CryG_T Cuts at: 458 692 824 1503 2607 3584 458 Size: 234 132 679 1104 977 1025 Fragments arranged by size: 1104 1025 977 679 234 132 AgeI A'CCGG_T Cuts at: 2539 2539 Size: 4151 AhdI GACnn_n'nnGTC Cuts at: 123 123 Size: 4151 AlwNI CAG_nnn'CTG Cuts at: 975 1456 3023 975 Size: 481 1567 2103 Fragments arranged by size: 2103 1567 481 ApaI G_GGCC'C Cuts at: 648 648 Size: 4151 ApaLI G'TGCA_C Cuts at: 2921 2921 Size: 4151 ApoI r'AATT_y Cuts at: 1155 3504 3760 1155 Size: 2349 256 1546 Fragments arranged by size: 2349 1546 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4151 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4151 AvaI C'yCGr_G Cuts at: 128 649 1171 128 Size: 521 522 3108 Fragments arranged by size: 3108 522 521 BamHI G'GATC_C Cuts at: 503 1176 503 Size: 673 3478 BanI G'GyrC_C Cuts at: 1167 1581 1829 1864 1167 Size: 414 248 35 3454 Fragments arranged by size: 3454 414 248 35 BanII G_rGCy'C Cuts at: 601 648 1165 2195 601 Size: 47 517 1030 2557 Fragments arranged by size: 2557 1030 517 47 BbeI G_GCGC'C Cuts at: 1585 1833 1585 Size: 248 3903 BbsI GAAGACnn'nnnn_ Cuts at: 826 826 Size: 4151 BciVI GTATCCnnnnn_n' Cuts at: 2049 2810 4151 2049 Size: 761 1341 2049 Fragments arranged by size: 2049 1341 761 BclI T'GATC_A Cuts at: 765 1671 765 Size: 906 3245 BglI GCCn_nnn'nGGC Cuts at: 970 970 Size: 4151 BglII A'GATC_T Cuts at: 915 1666 3703 915 Size: 751 2037 1363 Fragments arranged by size: 2037 1363 751 BlpI GC'TnA_GC Cuts at: 1263 1263 Size: 4151 Bme1580I G_kGCm'C Cuts at: 648 799 1776 1869 2925 648 Size: 151 977 93 1056 1874 Fragments arranged by size: 1874 1056 977 151 93 BmgBI CAC'GTC Cuts at: 120 120 Size: 4151 BmrI ACTGGGnnnn_n' Cuts at: 1363 1444 1779 3923 1363 Size: 81 335 2144 1591 Fragments arranged by size: 2144 1591 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2518 2518 Size: 4151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1327 2206 2698 2996 1327 Size: 879 492 298 2482 Fragments arranged by size: 2482 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 340 3855 340 Size: 3515 636 BsaAI yAC'GTr Cuts at: 1504 1504 Size: 4151 BsaBI GATnn'nnATC Cuts at: 6 1665 3702 6 Size: 1659 2037 455 Fragments arranged by size: 2037 1659 455 BsaHI Gr'CG_yC Cuts at: 1582 1830 2532 4028 1582 Size: 248 702 1496 1705 Fragments arranged by size: 1705 1496 702 248 BsaWI w'CCGG_w Cuts at: 1340 1861 2539 2813 2960 3371 1340 Size: 521 678 274 147 411 2120 Fragments arranged by size: 2120 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 157 187 157 Size: 30 4121 BseYI C'CCAG_C Cuts at: 1576 2334 2911 3407 1576 Size: 758 577 496 2320 Fragments arranged by size: 2320 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 914 914 Size: 4151 BsiEI CG_ry'CG Cuts at: 104 1693 1739 2947 104 Size: 1589 46 1208 1308 Fragments arranged by size: 1589 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 954 1165 1943 2133 2925 954 Size: 211 778 190 792 2180 Fragments arranged by size: 2180 792 778 211 190 BsmBI CGTCTCn'nnnn_ Cuts at: 896 896 Size: 4151 Bsp1286I G_dGCh'C Cuts at: 601 648 799 954 1165 1776 1869 1943 2133 Size: 47 151 155 211 611 93 74 190 Cuts at: 2133 2195 2925 601 Size: 62 730 1827 Fragments arranged by size: 1827 730 611 211 190 155 151 93 74 62 47 BspEI T'CCGG_A Cuts at: 3371 3371 Size: 4151 BspHI T'CATG_A Cuts at: 3327 3327 Size: 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1201 1415 1717 2098 1201 Size: 214 302 381 3254 Fragments arranged by size: 3254 381 302 214 BsrBI CCG'CTC Cuts at: 50 2443 2497 3981 4146 50 Size: 2393 54 1484 165 55 Fragments arranged by size: 2393 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2063 2063 Size: 4151 BsrFI r'CCGG_y Cuts at: 2149 2330 2539 2149 Size: 181 209 3761 Fragments arranged by size: 3761 209 181 BssSI C'ACGA_G Cuts at: 2422 2780 2422 Size: 358 3793 BstAPI GCAn_nnn'nTGC Cuts at: 1528 1528 Size: 4151 BstBI TT'CG_AA Cuts at: 2512 2512 Size: 4151 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 4151 BstYI r'GATC_y Cuts at: 1 503 516 915 1176 1643 1666 2001 2247 Size: 502 13 399 261 467 23 335 246 Cuts at: 2247 3259 3345 3357 3703 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 502 467 449 399 346 335 261 246 86 23 13 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2101 2282 2445 2101 Size: 181 163 3807 Fragments arranged by size: 3807 181 163 BtsI GCAGTG_nn' Cuts at: 1394 1394 Size: 4151 DraI TTT'AAA Cuts at: 1150 1150 Size: 4151 DrdI GACnn_nn'nnGTC Cuts at: 1040 1857 2715 1040 Size: 817 858 2476 Fragments arranged by size: 2476 858 817 EaeI y'GGCC_r Cuts at: 581 817 962 1000 1736 1910 2301 2328 3673 Size: 236 145 38 736 174 391 27 1345 Cuts at: 3673 581 Size: 1059 Fragments arranged by size: 1345 1059 736 391 236 174 145 38 27 EagI C'GGCC_G Cuts at: 1736 1736 Size: 4151 EarI CTCTTCn'nnn_ Cuts at: 476 2174 2384 476 Size: 1698 210 2243 Fragments arranged by size: 2243 1698 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2359 2669 2815 3961 2359 Size: 310 146 1146 2549 Fragments arranged by size: 2549 1146 310 146 EcoICRI GAG'CTC Cuts at: 1163 1163 Size: 4151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 873 1976 2408 3155 3423 873 Size: 1103 432 747 268 1601 Fragments arranged by size: 1601 1103 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 163 163 Size: 4151 EcoO109I rG'GnC_Cy Cuts at: 645 1290 645 Size: 645 3506 EcoRI G'AATT_C Cuts at: 1155 1155 Size: 4151 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 171 755 2170 171 Size: 584 1415 2152 Fragments arranged by size: 2152 1415 584 FspI TGC'GCA Cuts at: 1932 1932 Size: 4151 HaeII r_GCGC'y Cuts at: 1585 1833 2855 3834 1585 Size: 248 1022 979 1902 Fragments arranged by size: 1902 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 109 141 1993 2023 2025 2055 109 Size: 32 1852 30 2 30 2205 Fragments arranged by size: 2205 1852 32 30 30 2 HincII GTy'rAC Cuts at: 1190 1190 Size: 4151 KasI G'GCGC_C Cuts at: 1581 1829 1581 Size: 248 3903 KpnI G_GTAC'C Cuts at: 1171 1171 Size: 4151 MluI A'CGCG_T Cuts at: 3584 3584 Size: 4151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2822 3006 2822 Size: 184 3967 MscI TGG'CCA Cuts at: 583 1912 583 Size: 1329 2822 MslI CAynn'nnrTG Cuts at: 463 1017 2140 2267 463 Size: 554 1123 127 2347 Fragments arranged by size: 2347 1123 554 127 MspA1I CmG'CkG Cuts at: 1026 1262 1576 1936 2949 3194 1026 Size: 236 314 360 1013 245 1983 Fragments arranged by size: 1983 1013 360 314 245 236 NaeI GCC'GGC Cuts at: 2332 2332 Size: 4151 NarI GG'CG_CC Cuts at: 1582 1830 1582 Size: 248 3903 NcoI C'CATG_G Cuts at: 107 107 Size: 4151 NgoMIV G'CCGG_C Cuts at: 2330 2330 Size: 4151 NspI r_CATG'y Cuts at: 462 696 828 1204 1704 2611 462 Size: 234 132 376 500 907 2002 Fragments arranged by size: 2002 907 500 376 234 132 PciI A'CATG_T Cuts at: 458 692 824 2607 458 Size: 234 132 1783 2002 Fragments arranged by size: 2002 1783 234 132 PflMI CCAn_nnn'nTGG Cuts at: 1015 1015 Size: 4151 PmeI GTTT'AAAC Cuts at: 1150 1150 Size: 4151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3290 3322 3290 Size: 32 4119 PsiI TTA'TAA Cuts at: 409 409 Size: 4151 PspOMI G'GGCC_C Cuts at: 644 644 Size: 4151 PstI C_TGCA'G Cuts at: 1198 1883 1198 Size: 685 3466 PvuI CG_AT'CG Cuts at: 104 104 Size: 4151 PvuII CAG'CTG Cuts at: 1026 1576 1936 1026 Size: 550 360 3241 Fragments arranged by size: 3241 550 360 RsrII CG'GwC_CG Cuts at: 2346 2346 Size: 4151 SacI G_AGCT'C Cuts at: 1165 1165 Size: 4151 SalI G'TCGA_C Cuts at: 1188 1188 Size: 4151 SapI GCTCTTCn'nnn_ Cuts at: 2174 2384 2174 Size: 210 3941 SbfI CC_TGCA'GG Cuts at: 1198 1198 Size: 4151 ScaI AGT'ACT Cuts at: 3668 3668 Size: 4151 SexAI A'CCwGG_T Cuts at: 631 631 Size: 4151 SfcI C'TryA_G Cuts at: 33 1194 1879 2872 3063 33 Size: 1161 685 993 191 1121 Fragments arranged by size: 1161 1121 993 685 191 SfoI GGC'GCC Cuts at: 1583 1831 1583 Size: 248 3903 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4151 SmaI CCC'GGG Cuts at: 1173 1173 Size: 4151 SmlI C'TyrA_G Cuts at: 285 1306 2221 2713 2975 285 Size: 1021 915 492 262 1461 Fragments arranged by size: 1461 1021 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4151 SphI G_CATG'C Cuts at: 1204 1704 1204 Size: 500 3651 SspI AAT'ATT Cuts at: 366 487 782 366 Size: 121 295 3735 Fragments arranged by size: 3735 295 121 StuI AGG'CCT Cuts at: 1108 1108 Size: 4151 StyI C'CwwG_G Cuts at: 107 159 1285 1639 107 Size: 52 1126 354 2619 Fragments arranged by size: 2619 1126 354 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1734 1867 2326 2535 1734 Size: 133 459 209 3350 Fragments arranged by size: 3350 459 209 133 TatI w'GTAC_w Cuts at: 3666 3666 Size: 4151 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 663 695 3668 3700 663 Size: 32 2973 32 1114 Fragments arranged by size: 2973 1114 32 32 Tth111I GACn'n_nGTC Cuts at: 1948 1948 Size: 4151 XbaI T'CTAG_A Cuts at: 399 1182 399 Size: 783 3368 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1646 1646 Size: 4151 XmaI C'CCGG_G Cuts at: 1171 1171 Size: 4151 XmnI GAAnn'nnTTC Cuts at: 988 988 Size: 4151 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI