Restriction map for pF1KB3039
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BbrPI       BcgI        BlnI        BsaI        BsiEI       
BsiWI       BspDI       BspLU11I    BsrBI       BsrFI       
BssSI       Bst1107I    BstAPI      BstBI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
FseI        FspI        HpaI        KpnI        KspI        
MluI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    StuI        SwaI        Tth111I     
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        623  TCCGGA               T^CCGGA
AcyI          146  GGCGCC               G[GA]^CG[CT]C
AflII        2515  CTTAAG               C^TTAAG
AlwNI        1283  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI        1174  GTGCTC               G[AT]GC[AT]^C
BbeI          146  GGCGCC               GGCGC^C
BbsI         2041  GAAGAC               GAAGAC(2/6)
BbvCI        1958  CCTCAGC              CCTCAGC(-5/-2)
BciVI         335  GGATAC               GTATCC(6/5)
BfrI         2515  CTTAAG               C^TTAAG
BglI         2836  GCCGACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1881  AGATCT               A^GATCT
BlpI          541  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      541  GCTCAGC              GC^T[ACGT]AGC
BpuAI        2041  GAAGAC               GAAGAC(2/6)
BsaHI         146  GGCGCC               G[GA]^CG[CT]C
BseAI         623  TCCGGA               T^CCGGA
BsiHKAI      1174  GTGCTC               G[AT]GC[AT]^C
Bsp1407I     3041  TGTACA               T^GTACA
BspEI         623  TCCGGA               T^CCGGA
BspHI        2178  TCATGA               T^CATGA
BsrGI        3041  TGTACA               T^GTACA
BssHII       2521  GCGCGC               G^CGCGC
BstEII       2187  GGTGACC              G^GT[ACGT]ACC
BstPI        2187  GGTGACC              G^GT[ACGT]ACC
CelII         541  GCTCAGC              GC^T[ACGT]AGC
EcoO65I      2187  GGTGACC              G^GT[ACGT]ACC
EcoT22I       717  ATGCAT               ATGCA^T
HaeII         146  GGCGCC               [GA]GCGC^[CT]
Hin1I         146  GGCGCC               G[GA]^CG[CT]C
KasI          146  GGCGCC               G^GCGCC
MfeI         2311  CAATTG               C^AATTG
MroI          623  TCCGGA               T^CCGGA
MunI         2311  CAATTG               C^AATTG
NarI          146  GGCGCC               GG^CGCC
NsiI          717  ATGCAT               ATGCA^T
PpuMI         648  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI         2178  TCATGA               T^CATGA
SexAI        2123  ACCAGGT              A^CC[AT]GGT
SfoI          146  GGCGCC               GGC^GCC
SmaI         2846  CCCGGG               CCC^GGG
SphI          901  GCATGC               GCATG^C
SspBI        3041  TGTACA               T^GTACA
TfiI         2200  GAATC                G^A[AT]TC
XbaI          519  TCTAGA               T^CTAGA
XmaCI        2846  CCCGGG               C^CCGGG
XmaI         2846  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII        748  ACGTGT               A^C[GA][CT]GT
              773  ACACGT              
AvaI         2846  CCCGGG               C^[CT]CG[GA]G
             2957  CCCGAG              
BamHI         207  GGATCC               G^GATCC
             1238  GGATCC              
BanI           64  GGTGCC               G^G[CT][GA]CC
              146  GGCGCC              
BanII         157  GAGCCC               G[GA]GC[CT]^C
             1009  GGGCTC              
BsaAI        1831  TACGTG               [CT]AC^GT[GA]
             3304  TACGTG              
BsaBI          78  GATCTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3012  GATGGCCATC          
BsaWI         623  TCCGGA               [AT]^CCGG[AT]
             1235  ACCGGA              
BsmBI        1599  GAGACG               CGTCTC(1/5)
             2761  GAGACG              
BsoBI        2846  CCCGGG               C^[CT]CG[GA]G
             2957  CCCGAG              
BstXI         549  CCAGGATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3001  CCAGTGCGCTGG        
DraII         648  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2099  AGGGCCT             
DsaI           68  CCATGG               C^C[GA][CT]GG
             2718  CCATGG              
EcoO109I      648  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2099  AGGGCCT             
FauI         3119  GCGGG                CCCGC(4/6)
             3225  GCGGG               
HincII        356  GTCAAC               GT[CT]^[GA]AC
             1849  GTCAAC              
HindII        356  GTCAAC               GT[CT]^[GA]AC
             1849  GTCAAC              
MamI           78  GATCTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3012  GATGGCCATC          
MslI          345  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2050  CACGTTGATG          
NcoI           68  CCATGG               C^CATGG
             2718  CCATGG              
NspI          901  GCATGC               [GA]CATG^[CT]
             2671  GCATGT              
PstI          886  CTGCAG               CTGCA^G
             1269  CTGCAG              
XcmI          699  CCATGAAGATACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2449  CCAGTGCTTGACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaII       BalI        BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoNI       
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       
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