(Circular) (Six-base) MAPSORT of: pF1KA1840.seq Check: 7778 from: 1 to: 10442 pF1KA1840 10442 bp With 182 enzymes: SgfI * July 14, 2018 20:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 7423 7423 Size: 10442 AccI GT'mk_AC Cuts at: 1523 3128 3228 6081 7480 1523 Size: 1605 100 2853 1399 4485 Fragments arranged by size: 4485 2853 1605 1399 100 Acc65I G'GTAC_C Cuts at: 6697 7458 6697 Size: 761 9681 AclI AA'CG_TT Cuts at: 10282 10282 Size: 10442 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 2214 2768 2827 4275 4628 4776 4832 5098 Size: 199 554 59 1448 353 148 56 266 Cuts at: 5098 6343 6765 7321 7411 8267 8699 9446 9714 Size: 1245 422 556 90 856 432 747 268 Cuts at: 9714 2015 Size: 2743 Fragments arranged by size: 2743 1448 1245 856 747 556 554 432 422 353 268 266 199 148 90 59 56 AflII C'TTAA_G Cuts at: 4314 6238 6904 4314 Size: 1924 666 7852 Fragments arranged by size: 7852 1924 666 AflIII A'CryG_T Cuts at: 839 7794 8898 9875 839 Size: 6955 1104 977 1406 Fragments arranged by size: 6955 1406 1104 977 AgeI A'CCGG_T Cuts at: 8830 8830 Size: 10442 AleI CACnn'nnGTG Cuts at: 1361 4585 1361 Size: 3224 7218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2849 5471 2849 Size: 2622 7820 AlwNI CAG_nnn'CTG Cuts at: 1545 2555 6250 7747 9314 1545 Size: 1010 3695 1497 1567 2673 Fragments arranged by size: 3695 2673 1567 1497 1010 ApaI G_GGCC'C Cuts at: 355 355 Size: 10442 ApaLI G'TGCA_C Cuts at: 3507 9212 3507 Size: 5705 4737 ApoI r'AATT_y Cuts at: 457 740 963 1743 2286 2429 2629 2812 2921 Size: 283 223 780 543 143 200 183 109 Cuts at: 2921 3211 3699 4436 5316 5328 7154 7204 7446 Size: 290 488 737 880 12 1826 50 242 Cuts at: 7446 9795 10051 457 Size: 2349 256 848 Fragments arranged by size: 2349 1826 880 848 780 737 543 488 290 283 256 242 223 200 183 143 109 50 12 AseI AT'TA_AT Cuts at: 20 2048 3766 5075 20 Size: 2028 1718 1309 5387 Fragments arranged by size: 5387 2028 1718 1309 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 10442 AvaI C'yCGr_G Cuts at: 236 7462 236 Size: 7226 3216 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 788 821 788 Size: 33 10409 BamHI G'GATC_C Cuts at: 3441 6687 7467 3441 Size: 3246 780 6416 Fragments arranged by size: 6416 3246 780 BanI G'GyrC_C Cuts at: 160 198 4543 5472 5756 6697 6881 7458 7872 Size: 38 4345 929 284 941 184 577 414 Cuts at: 7872 8120 8155 160 Size: 248 35 2447 Fragments arranged by size: 4345 2447 941 929 577 414 284 248 184 38 35 BanII G_rGCy'C Cuts at: 355 2670 3833 5567 6213 6461 7456 8486 355 Size: 2315 1163 1734 646 248 995 1030 2311 Fragments arranged by size: 2315 2311 1734 1163 1030 995 646 248 BbeI G_GCGC'C Cuts at: 5760 7876 8124 5760 Size: 2116 248 8078 Fragments arranged by size: 8078 2116 248 BbsI GAAGACnn'nnnn_ Cuts at: 842 2978 842 Size: 2136 8306 BbvCI CC'TCA_GC Cuts at: 7121 7121 Size: 10442 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 6802 6836 6802 Size: 34 10408 BciVI GTATCCnnnnn_n' Cuts at: 3018 5052 8340 9101 10442 3018 Size: 2034 3288 761 1341 3018 Fragments arranged by size: 3288 3018 2034 1341 761 BclI T'GATC_A Cuts at: 1803 1812 7962 1803 Size: 9 6150 4283 Fragments arranged by size: 6150 4283 9 BfrBI ATG'CAT Cuts at: 1284 3631 1284 Size: 2347 8095 BglI GCCn_nnn'nGGC Cuts at: 350 5380 350 Size: 5030 5412 BglII A'GATC_T Cuts at: 1017 2381 5128 5507 5582 7957 9994 1017 Size: 1364 2747 379 75 2375 2037 1465 Fragments arranged by size: 2747 2375 2037 1465 1364 379 75 BlpI GC'TnA_GC Cuts at: 5921 7554 5921 Size: 1633 8809 Bme1580I G_kGCm'C Cuts at: 163 355 3511 4349 4390 5450 5477 6011 6884 Size: 192 3156 838 41 1060 27 534 873 Cuts at: 6884 8067 8160 9216 163 Size: 1183 93 1056 1389 Fragments arranged by size: 3156 1389 1183 1060 1056 873 838 534 192 93 41 27 BmgBI CAC'GTC Cuts at: 5469 6494 5469 Size: 1025 9417 BmrI ACTGGGnnnn_n' Cuts at: 4291 4947 6557 7654 7735 8070 10214 4291 Size: 656 1610 1097 81 335 2144 4519 Fragments arranged by size: 4519 2144 1610 1097 656 335 81 BmtI G_CTAG'C Cuts at: 3278 7432 3278 Size: 4154 6288 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 4482 4727 5017 8809 3797 Size: 685 245 290 3792 5430 Fragments arranged by size: 5430 3792 685 290 245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 367 1189 2778 4522 4698 4846 4921 5509 5518 Size: 822 1589 1744 176 148 75 588 9 Cuts at: 5518 5997 6764 367 Size: 479 767 4045 Fragments arranged by size: 4045 1744 1589 822 767 588 479 176 148 75 9 Bpu10I CC'TnA_GC Cuts at: 6146 7121 6146 Size: 975 9467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 887 1583 2281 2362 3889 4678 5950 6856 7618 Size: 696 698 81 1527 789 1272 906 762 Cuts at: 7618 8497 8989 9287 887 Size: 879 492 298 2042 Fragments arranged by size: 2042 1527 1272 906 879 789 762 698 696 492 298 81 BsaI GGTCTCn'nnnn_ Cuts at: 1468 1529 10146 1468 Size: 61 8617 1764 Fragments arranged by size: 8617 1764 61 BsaAI yAC'GTr Cuts at: 2413 7795 2413 Size: 5382 5060 BsaBI GATnn'nnATC Cuts at: 6 2009 7956 9993 6 Size: 2003 5947 2037 455 Fragments arranged by size: 5947 2037 2003 455 BsaHI Gr'CG_yC Cuts at: 313 5757 7873 8121 8823 10319 313 Size: 5444 2116 248 702 1496 436 Fragments arranged by size: 5444 2116 1496 702 436 248 BsaWI w'CCGG_w Cuts at: 6167 7631 8152 8830 9104 9251 9662 6167 Size: 1464 521 678 274 147 411 6947 Fragments arranged by size: 6947 1464 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 3335 3365 3335 Size: 30 10412 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 924 2278 3503 3825 4471 5431 5506 6583 924 Size: 1354 1225 322 646 960 75 1077 4783 Fragments arranged by size: 4783 1354 1225 1077 960 646 322 75 BseYI C'CCAG_C Cuts at: 155 1127 1256 1673 2822 3446 4260 4962 5528 Size: 972 129 417 1149 624 814 702 566 Cuts at: 5528 6971 7867 8625 9202 9698 155 Size: 1443 896 758 577 496 899 Fragments arranged by size: 1443 1149 972 899 896 814 758 702 624 577 566 496 417 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 680 1270 1400 3975 4168 5872 680 Size: 590 130 2575 193 1704 5250 Fragments arranged by size: 5250 2575 1704 590 193 130 BsiEI CG_ry'CG Cuts at: 104 7984 8030 9238 104 Size: 7880 46 1208 1308 Fragments arranged by size: 7880 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1188 1687 3104 3511 5393 5856 6461 7456 8234 Size: 499 1417 407 1882 463 605 995 778 Cuts at: 8234 8424 9216 1188 Size: 190 792 2414 Fragments arranged by size: 2414 1882 1417 995 792 778 605 499 463 407 190 BsmI GAATG_Cn' Cuts at: 2986 4085 2986 Size: 1099 9343 Bsp1286I G_dGCh'C Cuts at: 163 355 1188 1687 2670 3104 3511 3833 4349 Size: 192 833 499 983 434 407 322 516 Cuts at: 4349 4390 5393 5450 5477 5567 5856 6011 6213 Size: 41 1003 57 27 90 289 155 202 Cuts at: 6213 6461 6884 7456 8067 8160 8234 8424 8486 Size: 248 423 572 611 93 74 190 62 Cuts at: 8486 9216 163 Size: 730 1389 Fragments arranged by size: 1389 1003 983 833 730 611 572 516 499 434 423 407 322 289 248 202 192 190 155 93 90 74 62 57 41 27 BspEI T'CCGG_A Cuts at: 6167 9662 6167 Size: 3495 6947 BspHI T'CATG_A Cuts at: 1622 2066 9618 1622 Size: 444 7552 2446 Fragments arranged by size: 7552 2446 444 BspMI ACCTGCnnnn'nnnn_ Cuts at: 7423 7492 7706 8008 8389 7423 Size: 69 214 302 381 9476 Fragments arranged by size: 9476 381 302 214 69 BsrBI CCG'CTC Cuts at: 50 8734 8788 10272 10437 50 Size: 8684 54 1484 165 55 Fragments arranged by size: 8684 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 517 3816 4619 6477 8354 517 Size: 3299 803 1858 1877 2605 Fragments arranged by size: 3299 2605 1877 1858 803 BsrFI r'CCGG_y Cuts at: 145 5801 8440 8621 8830 145 Size: 5656 2639 181 209 1757 Fragments arranged by size: 5656 2639 1757 209 181 BsrGI T'GTAC_A Cuts at: 2115 2115 Size: 10442 BssSI C'ACGA_G Cuts at: 6262 6811 8713 9071 6262 Size: 549 1902 358 7633 Fragments arranged by size: 7633 1902 549 358 BstAPI GCAn_nnn'nTGC Cuts at: 3108 4385 5042 7819 3108 Size: 1277 657 2777 5731 Fragments arranged by size: 5731 2777 1277 657 BstBI TT'CG_AA Cuts at: 2058 8803 2058 Size: 6745 3697 BstEII G'GTnAC_C Cuts at: 1231 1231 Size: 10442 BstXI CCAn_nnnn'nTGG Cuts at: 1647 4469 4899 5402 6117 1647 Size: 2822 430 503 715 5972 Fragments arranged by size: 5972 2822 715 503 430 BstYI r'GATC_y Cuts at: 1 770 1017 2381 3252 3441 4662 5128 5507 Size: 769 247 1364 871 189 1221 466 379 Cuts at: 5507 5582 5883 5897 6687 7467 7934 7957 8292 Size: 75 301 14 790 780 467 23 335 Cuts at: 8292 8538 9550 9636 9648 9994 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1364 1221 1012 871 790 780 769 467 466 449 379 346 335 301 247 246 189 86 75 23 14 12 Bsu36I CC'TnA_GG Cuts at: 1037 5862 5956 1037 Size: 4825 94 5523 Fragments arranged by size: 5523 4825 94 BtgI C'CryG_G Cuts at: 107 164 170 1189 1629 6063 6432 107 Size: 57 6 1019 440 4434 369 4117 Fragments arranged by size: 4434 4117 1019 440 369 57 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 232 8392 8573 8736 232 Size: 8160 181 163 1938 Fragments arranged by size: 8160 1938 181 163 BtsI GCAGTG_nn' Cuts at: 710 926 1412 1495 4499 4948 5844 7685 710 Size: 216 486 83 3004 449 896 1841 3467 Fragments arranged by size: 3467 3004 1841 896 486 449 216 83 DraI TTT'AAA Cuts at: 1613 2198 2306 2435 7441 1613 Size: 585 108 129 5006 4614 Fragments arranged by size: 5006 4614 585 129 108 DraIII CAC_nnn'GTG Cuts at: 1511 6374 1511 Size: 4863 5579 DrdI GACnn_nn'nnGTC Cuts at: 8148 9006 8148 Size: 858 9584 EaeI y'GGCC_r Cuts at: 167 1088 2936 3727 3929 5171 7029 8027 8201 Size: 921 1848 791 202 1242 1858 998 174 Cuts at: 8201 8592 8619 9964 167 Size: 391 27 1345 645 Fragments arranged by size: 1858 1848 1345 1242 998 921 791 645 391 202 174 27 EagI C'GGCC_G Cuts at: 8027 8027 Size: 10442 EarI CTCTTCn'nnn_ Cuts at: 1598 1750 1844 3502 4831 5008 6266 7238 8465 Size: 152 94 1658 1329 177 1258 972 1227 Cuts at: 8465 8675 1598 Size: 210 3365 Fragments arranged by size: 3365 1658 1329 1258 1227 972 210 177 152 94 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 132 8650 8960 9106 10252 132 Size: 8518 310 146 1146 322 Fragments arranged by size: 8518 1146 322 310 146 EcoICRI GAG'CTC Cuts at: 6459 7454 6459 Size: 995 9447 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 367 1189 2015 2214 2768 2778 2827 4275 4522 Size: 822 826 199 554 10 49 1448 247 Cuts at: 4522 4628 4698 4776 4832 4846 4921 5098 5509 Size: 106 70 78 56 14 75 177 411 Cuts at: 5509 5518 5997 6343 6764 6765 7321 7411 8267 Size: 9 479 346 421 1 556 90 856 Cuts at: 8267 8699 9446 9714 367 Size: 432 747 268 1095 Fragments arranged by size: 1448 1095 856 826 822 747 556 554 479 432 421 411 346 268 247 199 177 106 90 78 75 70 56 49 14 10 9 1 EcoNI CCTnn'n_nnAGG Cuts at: 1028 3854 7403 1028 Size: 2826 3549 4067 Fragments arranged by size: 4067 3549 2826 EcoO109I rG'GnC_Cy Cuts at: 351 352 1033 4506 6087 6234 6909 7395 7581 Size: 1 681 3473 1581 147 675 486 186 Cuts at: 7581 351 Size: 3212 Fragments arranged by size: 3473 3212 1581 681 675 486 186 147 1 EcoRI G'AATT_C Cuts at: 740 7446 740 Size: 6706 3736 EcoRV GAT'ATC Cuts at: 546 546 Size: 10442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1116 2403 4069 4202 4340 4837 6675 7192 8461 Size: 1287 1666 133 138 497 1838 517 1269 Cuts at: 8461 1116 Size: 3097 Fragments arranged by size: 3097 1838 1666 1287 1269 517 497 138 133 FspI TGC'GCA Cuts at: 250 8223 250 Size: 7973 2469 HaeII r_GCGC'y Cuts at: 2192 5424 5760 7876 8124 9146 10125 2192 Size: 3232 336 2116 248 1022 979 2509 Fragments arranged by size: 3232 2509 2116 1022 979 336 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1314 1346 1610 1642 1707 1739 1804 1836 2226 Size: 32 264 32 65 32 65 32 390 Cuts at: 2226 2258 3335 3367 4258 4290 5719 5751 5889 Size: 32 1077 32 891 32 1429 32 138 Cuts at: 5889 5921 6074 6106 6834 6866 8284 8314 8316 Size: 32 153 32 728 32 1418 30 2 Cuts at: 8316 8346 1314 Size: 30 3410 Fragments arranged by size: 3410 1429 1418 1077 891 728 390 264 153 138 65 65 32 32 32 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1065 3229 6082 7481 1065 Size: 2164 2853 1399 4026 Fragments arranged by size: 4026 2853 2164 1399 HindIII A'AGCT_T Cuts at: 2500 4927 4969 5029 2500 Size: 2427 42 60 7913 Fragments arranged by size: 7913 2427 60 42 HpaI GTT'AAC Cuts at: 1065 1065 Size: 10442 KasI G'GCGC_C Cuts at: 5756 7872 8120 5756 Size: 2116 248 8078 Fragments arranged by size: 8078 2116 248 KpnI G_GTAC'C Cuts at: 6701 7462 6701 Size: 761 9681 MfeI C'AATT_G Cuts at: 1933 2371 1933 Size: 438 10004 MluI A'CGCG_T Cuts at: 9875 9875 Size: 10442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 962 6389 6665 6855 6905 9113 9297 962 Size: 5427 276 190 50 2208 184 2107 Fragments arranged by size: 5427 2208 2107 276 190 184 50 MscI TGG'CCA Cuts at: 1090 2938 3931 5173 7031 8203 1090 Size: 1848 993 1242 1858 1172 3329 Fragments arranged by size: 3329 1858 1848 1242 1172 993 MslI CAynn'nnrTG Cuts at: 1361 1634 1667 2123 2831 4585 7288 8431 8558 Size: 273 33 456 708 1754 2703 1143 127 Cuts at: 8558 1361 Size: 3245 Fragments arranged by size: 3245 2703 1754 1143 708 456 273 127 33 MspA1I CmG'CkG Cuts at: 166 246 1545 1731 3792 6363 6820 6888 6984 Size: 80 1299 186 2061 2571 457 68 96 Cuts at: 6984 7553 7867 8227 9240 9485 166 Size: 569 314 360 1013 245 1123 Fragments arranged by size: 2571 2061 1299 1123 1013 569 457 360 314 245 186 96 80 68 NaeI GCC'GGC Cuts at: 147 8623 147 Size: 8476 1966 NarI GG'CG_CC Cuts at: 5757 7873 8121 5757 Size: 2116 248 8078 Fragments arranged by size: 8078 2116 248 NcoI C'CATG_G Cuts at: 107 170 1189 1629 6063 6432 107 Size: 63 1019 440 4434 369 4117 Fragments arranged by size: 4434 4117 1019 440 369 63 NdeI CA'TA_TG Cuts at: 801 801 Size: 10442 NgoMIV G'CCGG_C Cuts at: 145 8621 145 Size: 8476 1966 NheI G'CTAG_C Cuts at: 3274 7428 3274 Size: 4154 6288 NruI TCG'CGA Cuts at: 130 130 Size: 10442 NsiI A_TGCA'T Cuts at: 1286 3633 1286 Size: 2347 8095 NspI r_CATG'y Cuts at: 843 1488 1702 3869 5387 5705 6540 6789 7428 Size: 645 214 2167 1518 318 835 249 639 Cuts at: 7428 7495 7995 8902 843 Size: 67 500 907 2383 Fragments arranged by size: 2383 2167 1518 907 835 645 639 500 318 249 214 67 PasI CC'CwG_GG Cuts at: 1479 6857 1479 Size: 5378 5064 PciI A'CATG_T Cuts at: 839 8898 839 Size: 8059 2383 PflMI CCAn_nnn'nTGG Cuts at: 2555 2555 Size: 10442 PfoI T'CCnGG_A Cuts at: 1196 1332 3758 6741 1196 Size: 136 2426 2983 4897 Fragments arranged by size: 4897 2983 2426 136 PmeI GTTT'AAAC Cuts at: 7441 7441 Size: 10442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4324 4356 9581 9613 4324 Size: 32 5225 32 5153 Fragments arranged by size: 5225 5153 32 32 PpuMI rG'GwC_Cy Cuts at: 1033 6087 7395 1033 Size: 5054 1308 4080 Fragments arranged by size: 5054 4080 1308 PsiI TTA'TAA Cuts at: 4802 5819 4802 Size: 1017 9425 PspOMI G'GGCC_C Cuts at: 351 351 Size: 10442 PstI C_TGCA'G Cuts at: 117 736 3113 4159 4226 5150 5851 7010 7489 Size: 619 2377 1046 67 924 701 1159 479 Cuts at: 7489 8174 117 Size: 685 2385 Fragments arranged by size: 2385 2377 1159 1046 924 701 685 619 479 67 PvuI CG_AT'CG Cuts at: 104 104 Size: 10442 PvuII CAG'CTG Cuts at: 246 1545 1731 3792 6363 6820 6888 7867 8227 Size: 1299 186 2061 2571 457 68 979 360 Cuts at: 8227 246 Size: 2461 Fragments arranged by size: 2571 2461 2061 1299 979 457 360 186 68 RsrII CG'GwC_CG Cuts at: 8637 8637 Size: 10442 SacI G_AGCT'C Cuts at: 6461 7456 6461 Size: 995 9447 SacII CC_GC'GG Cuts at: 167 167 Size: 10442 SalI G'TCGA_C Cuts at: 3227 6080 7479 3227 Size: 2853 1399 6190 Fragments arranged by size: 6190 2853 1399 SapI GCTCTTCn'nnn_ Cuts at: 1750 1844 4831 8465 8675 1750 Size: 94 2987 3634 210 3517 Fragments arranged by size: 3634 3517 2987 210 94 SbfI CC_TGCA'GG Cuts at: 7489 7489 Size: 10442 ScaI AGT'ACT Cuts at: 668 4288 9959 668 Size: 3620 5671 1151 Fragments arranged by size: 5671 3620 1151 SexAI A'CCwGG_T Cuts at: 3665 3665 Size: 10442 SfcI C'TryA_G Cuts at: 33 113 504 732 1303 1520 2099 2474 3109 Size: 80 391 228 571 217 579 375 635 Cuts at: 3109 3620 3683 3995 4155 4222 4936 5097 5146 Size: 511 63 312 160 67 714 161 49 Cuts at: 5146 5847 6523 7006 7485 8170 9163 9354 33 Size: 701 676 483 479 685 993 191 1121 Fragments arranged by size: 1121 993 714 701 685 676 635 579 571 511 483 479 391 375 312 228 217 191 161 160 80 67 63 49 SfoI GGC'GCC Cuts at: 5758 7874 8122 5758 Size: 2116 248 8078 Fragments arranged by size: 8078 2116 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 10442 SgrAI Cr'CCGG_yG Cuts at: 145 145 Size: 10442 SmaI CCC'GGG Cuts at: 238 7464 238 Size: 7226 3216 SmlI C'TyrA_G Cuts at: 902 1598 2260 2377 3904 4314 4657 5929 6238 Size: 696 662 117 1527 410 343 1272 309 Cuts at: 6238 6871 6904 7597 8512 9004 9266 902 Size: 633 33 693 915 492 262 2078 Fragments arranged by size: 2078 1527 1272 915 696 693 662 633 492 410 343 309 262 117 33 SpeI A'CTAG_T Cuts at: 67 5590 67 Size: 5523 4919 SphI G_CATG'C Cuts at: 1488 7495 7995 1488 Size: 6007 500 3935 Fragments arranged by size: 6007 3935 500 StuI AGG'CCT Cuts at: 2282 6217 7126 2282 Size: 3935 909 5598 Fragments arranged by size: 5598 3935 909 StyI C'CwwG_G Cuts at: 107 170 1189 1500 1629 2080 3202 5438 5477 Size: 63 1019 311 129 451 1122 2236 39 Cuts at: 5477 5834 6063 6110 6432 6947 7576 7930 107 Size: 357 229 47 322 515 629 354 2619 Fragments arranged by size: 2619 2236 1122 1019 629 515 451 357 354 322 311 229 129 63 47 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 998 4274 4438 5408 5542 5906 6212 8025 8158 Size: 3276 164 970 134 364 306 1813 133 Cuts at: 8158 8617 8826 998 Size: 459 209 2614 Fragments arranged by size: 3276 2614 1813 970 459 364 306 209 164 134 133 TatI w'GTAC_w Cuts at: 666 1240 2115 3974 4286 9957 666 Size: 574 875 1859 312 5671 1151 Fragments arranged by size: 5671 1859 1151 875 574 312 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1807 1839 1940 1972 4897 4929 9959 9991 1807 Size: 32 101 32 2925 32 5030 32 2258 Fragments arranged by size: 5030 2925 2258 101 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 7285 8239 7285 Size: 954 9488 XbaI T'CTAG_A Cuts at: 7473 7473 Size: 10442 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1340 4356 5536 6021 7937 1340 Size: 3016 1180 485 1916 3845 Fragments arranged by size: 3845 3016 1916 1180 485 XmaI C'CCGG_G Cuts at: 236 7462 236 Size: 7226 3216 XmnI GAAnn'nnTTC Cuts at: 1019 1019 Size: 10442 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AhdI AloI AscI AvrII BsiWI BsmBI BssHII BstZ17I ClaI CspCI FseI FspAI NotI PacI PmlI PshAI PspXI PsrI SanDI SfiI SnaBI SrfI SspI SwaI XhoI ZraI