Restriction map for pF1KA1398
 (Linear) (Six-base) MAPSORT of: KIAA1398.seq  Check: 3221  from: 1  to: 4680



KIAA1398.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3900    4680 
   Size:       3900     780
 
AatII G_ACGT'C

Cuts at:      0    3374    4680 
   Size:       3374    1306
 
AccI GT'mk_AC

Cuts at:      0      85    4680 
   Size:         85    4595
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656    2782    3760    4339    4454    4516    4591    4680 
   Size:        656    2126     978     579     115      62      75      89

  Fragments arranged by size: 

                2126     978     656     579     115      89      75      62
            
 
AflIII A'CryG_T

Cuts at:      0    4040    4680 
   Size:       4040     640
 
AleI CACnn'nnGTG

Cuts at:      0    2719    4680 
   Size:       2719    1961
 
AlwNI CAG_nnn'CTG

Cuts at:      0     580    2260    2775    2980    3048    4680 
   Size:        580    1680     515     205      68    1632

  Fragments arranged by size: 

                1680    1632     580     515     205      68
 
ApaI G_GGCC'C

Cuts at:      0     795     855     915     945     975    1005    1035    1065 
   Size:        795      60      60      30      30      30      30      30

Cuts at:   1065    1095    1185    1245    1275    1305    1335    1365    1395 
   Size:         30      90      60      30      30      30      30      30

Cuts at:   1395    1425    1455    1485    1605    1635    1695    1725    1755 
   Size:         30      30      30     120      30      60      30      30

Cuts at:   1755    1785    1815    1875    1905    2366    4269    4680 
   Size:         30      30      60      30     461    1903     411

  Fragments arranged by size: 

                1903     795     461     411     120      90      60      60
                  60      60      60      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30
 
ApoI r'AATT_y

Cuts at:      0     471    4680 
   Size:        471    4209
 
AscI GG'CGCG_CC

Cuts at:      0    4542    4680 
   Size:       4542     138
 
AvaI C'yCGr_G

Cuts at:      0    2699    3959    4680 
   Size:       2699    1260     721

  Fragments arranged by size: 

                2699    1260     721
 
BanI G'GyrC_C

Cuts at:      0     528     543     556    1210    2301    3931    4287    4669 
   Size:        528      15      13     654    1091    1630     356     382

Cuts at:   4669    4680 
   Size:         11

  Fragments arranged by size: 

                1630    1091     654     528     382     356      15      13
                  11
 
BanII G_rGCy'C

Cuts at:      0     306     645     795     855     885     915     945     975 
   Size:        306     339     150      60      30      30      30      30

Cuts at:    975    1005    1035    1065    1095    1125    1155    1185    1245 
   Size:         30      30      30      30      30      30      30      60

Cuts at:   1245    1275    1305    1335    1365    1395    1425    1455    1485 
   Size:         30      30      30      30      30      30      30      30

Cuts at:   1485    1605    1635    1695    1725    1755    1785    1815    1875 
   Size:        120      30      60      30      30      30      30      60

Cuts at:   1875    1905    1935    1965    1995    2025    2079    2296    2366 
   Size:         30      30      30      30      30      54     217      70

Cuts at:   2366    2627    2942    3467    3767    3950    4269    4680 
   Size:        261     315     525     300     183     319     411

  Fragments arranged by size: 

                 525     411     339     319     315     306     300     261
                 217     183     150     120      70      60      60      60
                  60      54      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30
 
BbeI G_GCGC'C

Cuts at:      0    3935    4291    4680 
   Size:       3935     356     389

  Fragments arranged by size: 

                3935     389     356
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    4327    4460    4579    4680 
   Size:       4327     133     119     101

  Fragments arranged by size: 

                4327     133     119     101
 
BbvCI CC'TCA_GC

Cuts at:      0    1115    1205    1895    4126    4680 
   Size:       1115      90     690    2231     554

  Fragments arranged by size: 

                2231    1115     690     554      90
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3391    3425    4183    4217    4680 
   Size:       3391      34     758      34     463

  Fragments arranged by size: 

                3391     758     463      34      34
 
BclI T'GATC_A

Cuts at:      0     252    4680 
   Size:        252    4428
 
BglI GCCn_nnn'nGGC

Cuts at:      0     880    1000    1030    1060    1090    1150    1240    1270 
   Size:        880     120      30      30      30      60      90      30

Cuts at:   1270    1330    1360    1390    1420    1450    1480    1600    1630 
   Size:         60      30      30      30      30      30     120      30

Cuts at:   1630    1690    1720    1750    1780    1810    1930    2671    3169 
   Size:         60      30      30      30      30     120     741     498

Cuts at:   3169    3385    3535    3556    3652    4680 
   Size:        216     150      21      96    1028

  Fragments arranged by size: 

                1028     880     741     498     216     150     120     120
                 120      96      90      60      60      60      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      21
 
BlpI GC'TnA_GC

Cuts at:      0    2941    4680 
   Size:       2941    1739
 
Bme1580I G_kGCm'C

Cuts at:      0     548     795     855     915     945     975    1005    1035 
   Size:        548     247      60      60      30      30      30      30

Cuts at:   1035    1065    1095    1185    1215    1245    1275    1305    1335 
   Size:         30      30      90      30      30      30      30      30

Cuts at:   1335    1365    1395    1425    1455    1485    1605    1635    1695 
   Size:         30      30      30      30      30     120      30      60

Cuts at:   1695    1725    1755    1785    1815    1875    1905    2122    2366 
   Size:         30      30      30      30      60      30     217     244

Cuts at:   2366    4269    4672    4680 
   Size:       1903     403       8

  Fragments arranged by size: 

                1903     548     403     247     244     217     120      90
                  60      60      60      60      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     325     544    2165    4680 
   Size:        325     219    1621    2515

  Fragments arranged by size: 

                2515    1621     325     219
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3786    4503    4680 
   Size:       3786     717     177

  Fragments arranged by size: 

                3786     717     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2830    3262    3361    3445    3466    3934    4351    4372 
   Size:       2830     432      99      84      21     468     417      21

Cuts at:   4372    4680 
   Size:        308

  Fragments arranged by size: 

                2830     468     432     417     308      99      84      21
                  21
 
Bpu10I CC'TnA_GC

Cuts at:      0    1115    1205    1895    2024    3466    4126    4680 
   Size:       1115      90     690     129    1442     660     554

  Fragments arranged by size: 

                1442    1115     690     660     554     129      90
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3400    4680 
   Size:       3400    1280
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2415    3872    4680 
   Size:       2415    1457     808

  Fragments arranged by size: 

                2415    1457     808
 
BsaAI yAC'GTr

Cuts at:      0    2757    4242    4680 
   Size:       2757    1485     438

  Fragments arranged by size: 

                2757    1485     438
 
BsaBI GATnn'nnATC

Cuts at:      0     257    4680 
   Size:        257    4423
 
BsaHI Gr'CG_yC

Cuts at:      0    3061    3371    3932    4288    4680 
   Size:       3061     310     561     356     392

  Fragments arranged by size: 

                3061     561     392     356     310
 
BsaWI w'CCGG_w

Cuts at:      0    2789    4680 
   Size:       2789    1891
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3412    3442    3766    3796    3898    3928    4027    4057 
   Size:       3412      30     324      30     102      30      99      30

Cuts at:   4057    4680 
   Size:        623

  Fragments arranged by size: 

                3412     623     324     102      99      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     390    3094    3265    3759    3817    3913    3916    3919 
   Size:        390    2704     171     494      58      96       3       3

Cuts at:   3919    3955    3958    4411    4495    4680 
   Size:         36       3     453      84     185

  Fragments arranged by size: 

                2704     494     453     390     185     171      96      84
                  58      36       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    2450    3302    4261    4269    4293    4680 
   Size:       2450     852     959       8      24     387

  Fragments arranged by size: 

                2450     959     852     387      24       8
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    2743    2788    3187    3559    4680 
   Size:        355    2388      45     399     372    1121

  Fragments arranged by size: 

                2388    1121     399     372     355      45
 
BsiEI CG_ry'CG

Cuts at:      0    3551    4082    4388    4680 
   Size:       3551     531     306     292

  Fragments arranged by size: 

                3551     531     306     292
 
BsiHKAI G_wGCw'C

Cuts at:      0    1845    2079    2627    2756    2942    3467    3817    3950 
   Size:       1845     234     548     129     186     525     350     133

Cuts at:   3950    4040    4680 
   Size:         90     640

  Fragments arranged by size: 

                1845     640     548     525     350     234     186     133
                 129      90
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3799    3853    4680 
   Size:       3799      54     827

  Fragments arranged by size: 

                3799     827      54
 
Bsp1286I G_dGCh'C

Cuts at:      0     306     548     645     795     855     885     915     945 
   Size:        306     242      97     150      60      30      30      30

Cuts at:    945     975    1005    1035    1065    1095    1125    1155    1185 
   Size:         30      30      30      30      30      30      30      30

Cuts at:   1185    1215    1245    1275    1305    1335    1365    1395    1425 
   Size:         30      30      30      30      30      30      30      30

Cuts at:   1425    1455    1485    1605    1635    1695    1725    1755    1785 
   Size:         30      30     120      30      60      30      30      30

Cuts at:   1785    1815    1845    1875    1905    1935    1965    1995    2025 
   Size:         30      30      30      30      30      30      30      30

Cuts at:   2025    2079    2122    2296    2366    2627    2756    2942    3467 
   Size:         54      43     174      70     261     129     186     525

Cuts at:   3467    3767    3817    3950    4040    4269    4672    4680 
   Size:        300      50     133      90     229     403       8

  Fragments arranged by size: 

                 525     403     306     300     261     242     229     186
                 174     150     133     129     120      97      90      70
                  60      60      54      50      43      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30       8
 
BspEI T'CCGG_A

Cuts at:      0    2789    4680 
   Size:       2789    1891
 
BspHI T'CATG_A

Cuts at:      0     255    4680 
   Size:        255    4425
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3033    3890    3900    4680 
   Size:       3033     857      10     780

  Fragments arranged by size: 

                3033     857     780      10
 
BsrFI r'CCGG_y

Cuts at:      0    4453    4680 
   Size:       4453     227
 
BssHII G'CGCG_C

Cuts at:      0    4542    4680 
   Size:       4542     138
 
BssSI C'ACGA_G

Cuts at:      0    2886    4551    4680 
   Size:       2886    1665     129

  Fragments arranged by size: 

                2886    1665     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2775    3048    3245    4044    4680 
   Size:       2775     273     197     799     636

  Fragments arranged by size: 

                2775     799     636     273     197
 
BstBI TT'CG_AA

Cuts at:      0     493    4680 
   Size:        493    4187
 
BstEII G'GTnAC_C

Cuts at:      0    2524    4680 
   Size:       2524    2156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    4116    4680 
   Size:       4116     564
 
BstYI r'GATC_y

Cuts at:      0    2465    2786    3711    4680 
   Size:       2465     321     925     969

  Fragments arranged by size: 

                2465     969     925     321
 
BtgI C'CryG_G

Cuts at:      0    2414    3267    3530    3545    3638    3758    4680 
   Size:       2414     853     263      15      93     120     922

  Fragments arranged by size: 

                2414     922     853     263     120      93      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    4208    4680 
   Size:       4208     472
 
BtsI GCAGTG_nn'

Cuts at:      0    3817    4680 
   Size:       3817     863
 
DraIII CAC_nnn'GTG

Cuts at:      0    3701    4680 
   Size:       3701     979
 
EaeI y'GGCC_r

Cuts at:      0    2576    2921    3194    3542    3548    4079    4385    4680 
   Size:       2576     345     273     348       6     531     306     295

  Fragments arranged by size: 

                2576     531     348     345     306     295     273       6
            
 
EagI C'GGCC_G

Cuts at:      0    3548    4079    4385    4680 
   Size:       3548     531     306     295

  Fragments arranged by size: 

                3548     531     306     295
 
EarI CTCTTCn'nnn_

Cuts at:      0     221    2552    2801    3883    3977    4228    4680 
   Size:        221    2331     249    1082      94     251     452

  Fragments arranged by size: 

                2331    1082     452     251     249     221      94
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     619     799    3172    3244    3307    3403    3871    4680 
   Size:        619     180    2373      72      63      96     468     809

  Fragments arranged by size: 

                2373     809     619     468     180      96      72      63
            
 
EcoICRI GAG'CTC

Cuts at:      0    2077    2625    2940    3465    3948    4680 
   Size:       2077     548     315     525     483     732

  Fragments arranged by size: 

                2077     732     548     525     483     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     656    2782    2830    3262    3361    3445    3466    3760 
   Size:        656    2126      48     432      99      84      21     294

Cuts at:   3760    3934    4339    4351    4372    4454    4516    4591    4680 
   Size:        174     405      12      21      82      62      75      89

  Fragments arranged by size: 

                2126     656     432     405     294     174      99      89
                  84      82      75      62      48      21      21      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2845    3103    3340    3586    4680 
   Size:       2845     258     237     246    1094

  Fragments arranged by size: 

                2845    1094     258     246     237
 
EcoO109I rG'GnC_Cy

Cuts at:      0     791     851     852     911     941     971    1001    1031 
   Size:        791      60       1      59      30      30      30      30

Cuts at:   1031    1061    1091    1181    1241    1271    1301    1331    1361 
   Size:         30      30      90      60      30      30      30      30

Cuts at:   1361    1391    1421    1451    1452    1481    1482    1511    1541 
   Size:         30      30      30       1      29       1      29      30

Cuts at:   1541    1571    1601    1631    1661    1691    1721    1751    1781 
   Size:         30      30      30      30      30      30      30      30

Cuts at:   1781    1811    1871    1901    2165    2195    2363    3045    3084 
   Size:         30      60      30     264      30     168     682      39

Cuts at:   3084    3333    3645    3727    4265    4266    4305    4680 
   Size:        249     312      82     538       1      39     375

  Fragments arranged by size: 

                 791     682     538     375     312     264     249     168
                  90      82      60      60      60      59      39      39
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      29      29       1       1       1       1
 
EcoRI G'AATT_C

Cuts at:      0     471    4680 
   Size:        471    4209
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4027    4680 
   Size:       4027     653
 
FspI TGC'GCA

Cuts at:      0    3238    4680 
   Size:       3238    1442
 
HaeII r_GCGC'y

Cuts at:      0    3099    3935    4091    4291    4680 
   Size:       3099     836     156     200     389

  Fragments arranged by size: 

                3099     836     389     200     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     237     269     603     635    2066    2098    2455    2487 
   Size:        237      32     334      32    1431      32     357      32

Cuts at:   2487    2778    2810    3412    3444    3766    3798    4680 
   Size:        291      32     602      32     322      32     882

  Fragments arranged by size: 

                1431     882     602     357     334     322     291     237
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0      86    4526    4680 
   Size:         86    4440     154

  Fragments arranged by size: 

                4440     154      86
 
HindIII A'AGCT_T

Cuts at:      0    2926    4680 
   Size:       2926    1754
 
HpaI GTT'AAC

Cuts at:      0    4526    4680 
   Size:       4526     154
 
KasI G'GCGC_C

Cuts at:      0    3931    4287    4680 
   Size:       3931     356     393

  Fragments arranged by size: 

                3931     393     356
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     349    4680 
   Size:        349    4331
 
MscI TGG'CCA

Cuts at:      0    2578    2923    3544    4680 
   Size:       2578     345     621    1136

  Fragments arranged by size: 

                2578    1136     621     345
 
MslI CAynn'nnrTG

Cuts at:      0    2719    4680 
   Size:       2719    1961
 
MspA1I CmG'CkG

Cuts at:      0     454    2227    2454    2553    2691    2775    2808    2832 
   Size:        454    1773     227      99     138      84      33      24

Cuts at:   2832    3003    3028    3054    3760    4317    4329    4371    4494 
   Size:        171      25      26     706     557      12      42     123

Cuts at:   4494    4590    4632    4680 
   Size:         96      42      48

  Fragments arranged by size: 

                1773     706     557     454     227     171     138     123
                  99      96      84      48      42      42      33      26
                  25      24      12
 
NaeI GCC'GGC

Cuts at:      0    4455    4680 
   Size:       4455     225
 
NarI GG'CG_CC

Cuts at:      0    3932    4288    4680 
   Size:       3932     356     392

  Fragments arranged by size: 

                3932     392     356
 
NcoI C'CATG_G

Cuts at:      0    3530    3638    4680 
   Size:       3530     108    1042

  Fragments arranged by size: 

                3530    1042     108
 
NdeI CA'TA_TG

Cuts at:      0     111    4680 
   Size:        111    4569
 
NgoMIV G'CCGG_C

Cuts at:      0    4453    4680 
   Size:       4453     227
 
NotI GC'GGCC_GC

Cuts at:      0    4079    4680 
   Size:       4079     601
 
NspI r_CATG'y

Cuts at:      0    2736    3873    4418    4680 
   Size:       2736    1137     545     262

  Fragments arranged by size: 

                2736    1137     545     262
 
PasI CC'CwG_GG

Cuts at:      0     861    4680 
   Size:        861    3819
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     580    4680 
   Size:        580    4100
 
PfoI T'CCnGG_A

Cuts at:      0    3278    3509    4349    4680 
   Size:       3278     231     840     331

  Fragments arranged by size: 

                3278     840     331     231
 
PmlI CAC'GTG

Cuts at:      0    2757    4680 
   Size:       2757    1923
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3306    3338    4680 
   Size:       3306      32    1342

  Fragments arranged by size: 

                3306    1342      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1511    1541    1571    1661    2165    2195    3045    4305 
   Size:       1511      30      30      90     504      30     850    1260

Cuts at:   4305    4680 
   Size:        375

  Fragments arranged by size: 

                1511    1260     850     504     375      90      30      30
                  30
 
PshAI GACnn'nnGTC

Cuts at:      0    2407    4680 
   Size:       2407    2273
 
PspOMI G'GGCC_C

Cuts at:      0     791     851     911     941     971    1001    1031    1061 
   Size:        791      60      60      30      30      30      30      30

Cuts at:   1061    1091    1181    1241    1271    1301    1331    1361    1391 
   Size:         30      90      60      30      30      30      30      30

Cuts at:   1391    1421    1451    1481    1601    1631    1691    1721    1751 
   Size:         30      30      30     120      30      60      30      30

Cuts at:   1751    1781    1811    1871    1901    2362    4265    4680 
   Size:         30      30      60      30     461    1903     415

  Fragments arranged by size: 

                1903     791     461     415     120      90      60      60
                  60      60      60      30      30      30      30      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30      30      30
 
PstI C_TGCA'G

Cuts at:      0    2780    2846    3053    3059    3092    3710    3824    3887 
   Size:       2780      66     207       6      33     618     114      63

Cuts at:   3887    4562    4625    4680 
   Size:        675      63      55

  Fragments arranged by size: 

                2780     675     618     207     114      66      63      63
                  55      33       6
 
PvuII CAG'CTG

Cuts at:      0     454    2227    2553    2691    2775    2808    2832    3028 
   Size:        454    1773     326     138      84      33      24     196

Cuts at:   3028    3054    4317    4329    4494    4590    4632    4680 
   Size:         26    1263      12     165      96      42      48

  Fragments arranged by size: 

                1773    1263     454     326     196     165     138      96
                  84      48      42      33      26      24      12
 
SacI G_AGCT'C

Cuts at:      0    2079    2627    2942    3467    3950    4680 
   Size:       2079     548     315     525     483     730

  Fragments arranged by size: 

                2079     730     548     525     483     315
 
SacII CC_GC'GG

Cuts at:      0    3761    4680 
   Size:       3761     919
 
SalI G'TCGA_C

Cuts at:      0      84    4680 
   Size:         84    4596
 
SanDI GG'GwC_CC

Cuts at:      0    1511    1541    1571    1661    2165    2195    4680 
   Size:       1511      30      30      90     504      30    2485

  Fragments arranged by size: 

                2485    1511     504      90      30      30      30
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3883    4228    4680 
   Size:       3883     345     452

  Fragments arranged by size: 

                3883     452     345
 
SexAI A'CCwGG_T

Cuts at:      0    4025    4680 
   Size:       4025     655
 
SfcI C'TryA_G

Cuts at:      0     464    2776    2842    3049    3055    3088    3706    3820 
   Size:        464    2312      66     207       6      33     618     114

Cuts at:   3820    3883    4558    4621    4680 
   Size:         63     675      63      59

  Fragments arranged by size: 

                2312     675     618     464     207     114      66      63
                  63      59      33       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1000    1030    1060    1090    1240    1270    1330    1360 
   Size:       1000      30      30      30     150      30      60      30

Cuts at:   1360    1390    1420    1450    1480    1600    1630    1690    1720 
   Size:         30      30      30      30     120      30      60      30

Cuts at:   1720    1750    1780    1810    3556    4680 
   Size:         30      30      30    1746    1124

  Fragments arranged by size: 

                1746    1124    1000     150     120      60      60      30
                  30      30      30      30      30      30      30      30
                  30      30      30      30      30
 
SfoI GGC'GCC

Cuts at:      0    3933    4289    4680 
   Size:       3933     356     391

  Fragments arranged by size: 

                3933     391     356
 
SmaI CCC'GGG

Cuts at:      0    2701    3961    4680 
   Size:       2701    1260     719

  Fragments arranged by size: 

                2701    1260     719
 
SmlI C'TyrA_G

Cuts at:      0    3415    4680 
   Size:       3415    1265
 
SphI G_CATG'C

Cuts at:      0    2736    3873    4680 
   Size:       2736    1137     807

  Fragments arranged by size: 

                2736    1137     807
 
StuI AGG'CCT

Cuts at:      0    3559    4486    4680 
   Size:       3559     927     194

  Fragments arranged by size: 

                3559     927     194
 
StyI C'CwwG_G

Cuts at:      0      23     407     521     560     711     741     771    2055 
   Size:         23     384     114      39     151      30      30    1284

Cuts at:   2055    2205    3020    3530    3593    3638    3923    4109    4680 
   Size:        150     815     510      63      45     285     186     571

  Fragments arranged by size: 

                1284     815     571     510     384     285     186     151
                 150     114      63      45      39      30      30      23
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     544    4680 
   Size:        544    4136
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3898    3930    4680 
   Size:       3898      32     750

  Fragments arranged by size: 

                3898     750      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2250    3106    4680 
   Size:       2250     856    1574

  Fragments arranged by size: 

                2250    1574     856
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2829    2892    3537    4246    4680 
   Size:       2829      63     645     709     434

  Fragments arranged by size: 

                2829     709     645     434      63
 
XmaI C'CCGG_G

Cuts at:      0    2699    3959    4680 
   Size:       2699    1260     721

  Fragments arranged by size: 

                2699    1260     721
 
XmnI GAAnn'nnTTC

Cuts at:      0    3321    4680 
   Size:       3321    1359
 
ZraI GAC'GTC

Cuts at:      0    3372    4680 
   Size:       3372    1308
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI    AlwNI     ApaI
     ApoI     AscI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI   PspOMI     PstI    PvuII     SacI    SacII     SalI    SanDI
     SapI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
    ApaLI     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI    BfrBI
    BglII    BmgBI     BmtI    BsiWI     BsmI    BsrBI    BsrDI    BsrGI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI    EcoRV     FseI
    FspAI     KpnI     MfeI     MluI     NheI     NruI     NsiI     PacI
     PciI     PmeI     PsiI    PspXI     PsrI     PvuI    RsrII     SbfI
     ScaI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI
     TatI     XbaI     XhoI
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