Restriction map for pF1KA1287
 (Circular) (Six-base) MAPSORT of: pF1KA1287.seq  Check: 9761  from: 1  to: 6725



pF1KA1287  6725 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    285     285 
   Size:       6725
 
AccI GT'mk_AC

Cuts at:   2650    3303    3763    2650 
   Size:        653     460    5612

  Fragments arranged by size: 

                5612     653     460
 
Acc65I G'GTAC_C

Cuts at:   3741    3741 
   Size:       6725
 
AclI AA'CG_TT

Cuts at:   6565    6565 
   Size:       6725
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     457     722    1340    1499    2599    2815    2975    4550 
   Size:        321     265     618     159    1100     216     160    1575

Cuts at:   4550    4982    5335    5997     136 
   Size:        432     353     662     864

  Fragments arranged by size: 

                1575    1100     864     662     618     432     353     321
                 265     216     160     159
 
AfeI AGC'GCT

Cuts at:   3027    3027 
   Size:       6725
 
AflIII A'CryG_T

Cuts at:    714    2744    3550    4077    5877    6158     714 
   Size:       2030     806     527    1800     281    1281

  Fragments arranged by size: 

                2030    1800    1281     806     527     281
 
AhdI GACnn_n'nnGTC

Cuts at:   1358    1358 
   Size:       6725
 
AleI CACnn'nnGTG

Cuts at:   2416    2416 
   Size:       6725
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3518    3518 
   Size:       6725
 
AlwNI CAG_nnn'CTG

Cuts at:   2314    4030    5468    2314 
   Size:       1716    1438    3571

  Fragments arranged by size: 

                3571    1716    1438
 
ApaLI G'TGCA_C

Cuts at:    272    1265    5563     272 
   Size:        993    4298    1434

  Fragments arranged by size: 

                4298    1434     993
 
ApoI r'AATT_y

Cuts at:    583    1679    1908    2176    2511    2531    3729    6078    6334 
   Size:       1096     229     268     335      20    1198    2349     256

Cuts at:   6334     583 
   Size:        974

  Fragments arranged by size: 

                2349    1198    1096     974     335     268     256     229
                  20
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6725
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6725
 
AvaI C'yCGr_G

Cuts at:   3745    3745 
   Size:       6725
 
BamHI G'GATC_C

Cuts at:   3622    3645    3750    3622 
   Size:         23     105    6597

  Fragments arranged by size: 

                6597     105      23
 
BanI G'GyrC_C

Cuts at:    727    3741    4155    4403    4438     727 
   Size:       3014     414     248      35    3014

  Fragments arranged by size: 

                3014    3014     414     248      35
 
BanII G_rGCy'C

Cuts at:    303     671    1214    3739    4769     303 
   Size:        368     543    2525    1030    2259

  Fragments arranged by size: 

                2525    2259    1030     543     368
 
BbeI G_GCGC'C

Cuts at:   4159    4407    4159 
   Size:        248    6477
 
BbsI GAAGACnn'nnnn_

Cuts at:    142     590    1666    2113    3062    3547     142 
   Size:        448    1076     447     949     485    3320

  Fragments arranged by size: 

                3320    1076     949     485     448     447
 
BciVI GTATCCnnnnn_n'

Cuts at:   4623    5679    6725    4623 
   Size:       1056    1046    4623

  Fragments arranged by size: 

                4623    1056    1046
 
BclI T'GATC_A

Cuts at:   2697    4245    2697 
   Size:       1548    5177
 
BfrBI ATG'CAT

Cuts at:   2893    3313    2893 
   Size:        420    6305
 
BglI GCCn_nnn'nGGC

Cuts at:   2586    2586 
   Size:       6725
 
BglII A'GATC_T

Cuts at:   4240    6277    4240 
   Size:       2037    4688
 
BlpI GC'TnA_GC

Cuts at:   1331    3143    3389    3837    1331 
   Size:       1812     246     448    4219

  Fragments arranged by size: 

                4219    1812     448     246
 
Bme1580I G_kGCm'C

Cuts at:    276    1269    4350    4443    5567     276 
   Size:        993    3081      93    1124    1434

  Fragments arranged by size: 

                3081    1434    1124     993      93
 
BmgBI CAC'GTC

Cuts at:   3553    3553 
   Size:       6725
 
BmrI ACTGGGnnnn_n'

Cuts at:   2395    3937    4018    4353    6497    2395 
   Size:       1542      81     335    2144    2623

  Fragments arranged by size: 

                2623    2144    1542     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1379    5092    1379 
   Size:       3713    3012
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    356    1162     356 
   Size:        806    5919
 
Bpu10I CC'TnA_GC

Cuts at:    302     421    2906    3236     302 
   Size:        119    2485     330    3791

  Fragments arranged by size: 

                3791    2485     330     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    877    1668    2180    3280    3901    4780    5494    5792     877 
   Size:        791     512    1100     621     879     714     298    1810

  Fragments arranged by size: 

                1810    1100     879     791     714     621     512     298
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1090    3435    3593    6429    1090 
   Size:       2345     158    2836    1386

  Fragments arranged by size: 

                2836    2345    1386     158
 
BsaAI yAC'GTr

Cuts at:   4078    4078 
   Size:       6725
 
BsaBI GATnn'nnATC

Cuts at:      6    1297    2170    4239    6276       6 
   Size:       1291     873    2069    2037     455

  Fragments arranged by size: 

                2069    2037    1291     873     455
 
BsaHI Gr'CG_yC

Cuts at:   1520    4156    4404    5106    6602    1520 
   Size:       2636     248     702    1496    1643

  Fragments arranged by size: 

                2636    1643    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1046    2862    3914    4435    5113    5524    5671    5945    1046 
   Size:       1816    1052     521     678     411     147     274    1826

  Fragments arranged by size: 

                1826    1816    1052     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1848    1878    2228    2258    2844    2874    3012    3042    1848 
   Size:         30     350      30     586      30     138      30    5531

  Fragments arranged by size: 

                5531     586     350     138      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    452    2974     452 
   Size:       2522    4203
 
BseYI C'CCAG_C

Cuts at:    296    1378    4150    4908    5573    5981     296 
   Size:       1082    2772     758     665     408    1040

  Fragments arranged by size: 

                2772    1082    1040     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2936    2936 
   Size:       6725
 
BsiEI CG_ry'CG

Cuts at:    104    3194    4267    4313    5543     104 
   Size:       3090    1073      46    1230    1286

  Fragments arranged by size: 

                3090    1286    1230    1073      46
 
BsiHKAI G_wGCw'C

Cuts at:    276     671    1269    2063    2790    3739    4517    4707    5567 
   Size:        395     598     794     727     949     778     190     860

Cuts at:   5567     276 
   Size:       1434

  Fragments arranged by size: 

                1434     949     860     794     778     727     598     395
                 190
 
BsmI GAATG_Cn'

Cuts at:   1154    2226    2339    1154 
   Size:       1072     113    5540

  Fragments arranged by size: 

                5540    1072     113
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1527    2248    1527 
   Size:        721    6004
 
Bsp1286I G_dGCh'C

Cuts at:    276     303     671    1214    1269    2063    2790    3739    4350 
   Size:         27     368     543      55     794     727     949     611

Cuts at:   4350    4443    4517    4707    4769    5567     276 
   Size:         93      74     190      62     798    1434

  Fragments arranged by size: 

                1434     949     798     794     727     611     543     368
                 190      93      74      62      55      27
 
BspEI T'CCGG_A

Cuts at:   1046    2862    1046 
   Size:       1816    4909
 
BspHI T'CATG_A

Cuts at:   5157    5157 
   Size:       6725
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    285    1683    3187    3775    3989    4291    4672     285 
   Size:       1398    1504     588     214     302     381    2338

  Fragments arranged by size: 

                2338    1504    1398     588     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2905    5017    5071    6555    6720      50 
   Size:       2855    2112      54    1484     165      55

  Fragments arranged by size: 

                2855    2112    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    188    3122    3137    4637     188 
   Size:       2934      15    1500    2276

  Fragments arranged by size: 

                2934    2276    1500      15
 
BsrFI r'CCGG_y

Cuts at:   4723    4904    4723 
   Size:        181    6544
 
BsrGI T'GTAC_A

Cuts at:    142    1901    3119    3654     142 
   Size:       1759    1218     535    3213

  Fragments arranged by size: 

                3213    1759    1218     535
 
BssSI C'ACGA_G

Cuts at:   4996    5704    4996 
   Size:        708    6017
 
BstAPI GCAn_nnn'nTGC

Cuts at:    271    1341    4102     271 
   Size:       1070    2761    2894

  Fragments arranged by size: 

                2894    2761    1070
 
BstBI TT'CG_AA

Cuts at:   5086    5086 
   Size:       6725
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3584    3584 
   Size:       6725
 
BstYI r'GATC_y

Cuts at:      1    2673    3129    3622    3645    3750    4217    4240    4575 
   Size:       2672     456     493      23     105     467      23     335

Cuts at:   4575    4821    5127    5139    5225    6277       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                2672    1052     493     467     456     449     335     306
                 246     105      86      23      23      12
 
BstZ17I GTA'TAC

Cuts at:   2651    3304    2651 
   Size:        653    6072
 
Bsu36I CC'TnA_GG

Cuts at:   2689    2689 
   Size:       6725
 
BtgI C'CryG_G

Cuts at:    374    2524     374 
   Size:       2150    4575
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4675    4856    5019    4675 
   Size:        181     163    6381

  Fragments arranged by size: 

                6381     181     163
 
BtsI GCAGTG_nn'

Cuts at:    450    3968     450 
   Size:       3518    3207
 
DraI TTT'AAA

Cuts at:   3724    3724 
   Size:       6725
 
DraIII CAC_nnn'GTG

Cuts at:    271    1818    3331     271 
   Size:       1547    1513    3665

  Fragments arranged by size: 

                3665    1547    1513
 
DrdI GACnn_nn'nnGTC

Cuts at:   4431    5775    4431 
   Size:       1344    5381
 
EaeI y'GGCC_r

Cuts at:    752    2900    4310    4484    4875    4902    6247     752 
   Size:       2148    1410     174     391      27    1345    1230

  Fragments arranged by size: 

                2148    1410    1345    1230     391     174      27
 
EagI C'GGCC_G

Cuts at:   4310    4310 
   Size:       6725
 
EarI CTCTTCn'nnn_

Cuts at:   1238    2078    2096    2951    4748    4958    1238 
   Size:        840      18     855    1797     210    3005

  Fragments arranged by size: 

                3005    1797     855     840     210      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2008    4933    5675    5821    6535    2008 
   Size:       2925     742     146     714    2198

  Fragments arranged by size: 

                2925    2198     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    669    3737     669 
   Size:       3068    3657
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     356     457     722    1162    1340    1499    2599    2815 
   Size:        220     101     265     440     178     159    1100     216

Cuts at:   2815    2975    4550    4982    5335    5997     136 
   Size:        160    1575     432     353     662     864

  Fragments arranged by size: 

                1575    1100     864     662     440     432     353     265
                 220     216     178     160     159     101
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1650    1650 
   Size:       6725
 
EcoO109I rG'GnC_Cy

Cuts at:    789     888    1719    3864     789 
   Size:         99     831    2145    3650

  Fragments arranged by size: 

                3650    2145     831      99
 
EcoRI G'AATT_C

Cuts at:   2511    2531    3729    2511 
   Size:         20    1198    5507

  Fragments arranged by size: 

                5507    1198      20
 
EcoRV GAT'ATC

Cuts at:   2758    2758 
   Size:       6725
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    844    1683    2107    2797    4744     844 
   Size:        839     424     690    1947    2825

  Fragments arranged by size: 

                2825    1947     839     690     424
 
FspI TGC'GCA

Cuts at:   4506    4506 
   Size:       6725
 
HaeII r_GCGC'y

Cuts at:   3029    4159    4407    5637    6408    3029 
   Size:       1130     248    1230     771    3346

  Fragments arranged by size: 

                3346    1230    1130     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    440     472     942     974    1344    1376    2658    2661    2690 
   Size:         32     470      32     370      32    1282       3      29

Cuts at:   2690    2693    3457    3489    4567    4597    4599    4629     440 
   Size:          3     764      32    1078      30       2      30    2536

  Fragments arranged by size: 

                2536    1282    1078     764     470     370      32      32
                  32      32      30      30      29       3       3       2
            
 
HincII GTy'rAC

Cuts at:    351    2486    3371    3764     351 
   Size:       2135     885     393    3312

  Fragments arranged by size: 

                3312    2135     885     393
 
HindIII A'AGCT_T

Cuts at:    704     974    3145     704 
   Size:        270    2171    4284

  Fragments arranged by size: 

                4284    2171     270
 
HpaI GTT'AAC

Cuts at:   2486    3371    2486 
   Size:        885    5840
 
KasI G'GCGC_C

Cuts at:   4155    4403    4155 
   Size:        248    6477
 
KpnI G_GTAC'C

Cuts at:   3745    3745 
   Size:       6725
 
MfeI C'AATT_G

Cuts at:   2834    2834 
   Size:       6725
 
MluI A'CGCG_T

Cuts at:   6158    6158 
   Size:       6725
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    599    1459    2826    5484    5668     599 
   Size:        860    1367    2658     184    1656

  Fragments arranged by size: 

                2658    1656    1367     860     184
 
MscI TGG'CCA

Cuts at:    754    4486     754 
   Size:       3732    2993
 
MslI CAynn'nnrTG

Cuts at:    910    2268    2416    4714    4841     910 
   Size:       1358     148    2298     127    2794

  Fragments arranged by size: 

                2794    2298    1358     148     127
 
MspA1I CmG'CkG

Cuts at:   1378    1458    1848    2395    2920    3836    4150    4510    5294 
   Size:         80     390     547     525     916     314     360     784

Cuts at:   5294    5539    1378 
   Size:        245    2564

  Fragments arranged by size: 

                2564     916     784     547     525     390     360     314
                 245      80
 
NaeI GCC'GGC

Cuts at:   4906    4906 
   Size:       6725
 
NarI GG'CG_CC

Cuts at:   4156    4404    4156 
   Size:        248    6477
 
NdeI CA'TA_TG

Cuts at:   2472    2472 
   Size:       6725
 
NgoMIV G'CCGG_C

Cuts at:   4904    4904 
   Size:       6725
 
NsiI A_TGCA'T

Cuts at:   2895    3315    2895 
   Size:        420    6305
 
NspI r_CATG'y

Cuts at:    718    1252    2748    3778    4278    5881     718 
   Size:        534    1496    1030     500    1603    1562

  Fragments arranged by size: 

                1603    1562    1496    1030     534     500
 
PciI A'CATG_T

Cuts at:    714    2744    5877     714 
   Size:       2030    3133    1562

  Fragments arranged by size: 

                3133    2030    1562
 
PflMI CCAn_nnn'nTGG

Cuts at:    937     937 
   Size:       6725
 
PmeI GTTT'AAAC

Cuts at:   3724    3724 
   Size:       6725
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5171    5203    5171 
   Size:         32    6693
 
PpuMI rG'GwC_Cy

Cuts at:   1719    1719 
   Size:       6725
 
PsiI TTA'TAA

Cuts at:   3614    3614 
   Size:       6725
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1961    1993    1961 
   Size:         32    6693
 
PstI C_TGCA'G

Cuts at:    642    1346    2207    3772    4457     642 
   Size:        704     861    1565     685    2910

  Fragments arranged by size: 

                2910    1565     861     704     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6725
 
PvuII CAG'CTG

Cuts at:   1378    1458    1848    2395    2920    4150    4510    1378 
   Size:         80     390     547     525    1230     360    3593

  Fragments arranged by size: 

                3593    1230     547     525     390     360      80
 
RsrII CG'GwC_CG

Cuts at:   4920    4920 
   Size:       6725
 
SacI G_AGCT'C

Cuts at:    671    3739     671 
   Size:       3068    3657
 
SalI G'TCGA_C

Cuts at:   3762    3762 
   Size:       6725
 
SanDI GG'GwC_CC

Cuts at:   1719    1719 
   Size:       6725
 
SapI GCTCTTCn'nnn_

Cuts at:   1238    2096    4748    4958    1238 
   Size:        858    2652     210    3005

  Fragments arranged by size: 

                3005    2652     858     210
 
SbfI CC_TGCA'GG

Cuts at:   3772    3772 
   Size:       6725
 
ScaI AGT'ACT

Cuts at:   1512    6242    1512 
   Size:       4730    1995
 
SfcI C'TryA_G

Cuts at:     33     638     686    1342    2203    3768    4453    5421    5612 
   Size:        605      48     656     861    1565     685     968     191

Cuts at:   5612      33 
   Size:       1146

  Fragments arranged by size: 

                1565    1146     968     861     685     656     605     191
                  48
 
SfoI GGC'GCC

Cuts at:   4157    4405    4157 
   Size:        248    6477
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6725
 
SmaI CCC'GGG

Cuts at:   3747    3747 
   Size:       6725
 
SmlI C'TyrA_G

Cuts at:    892    1647    2195    3259    3880    4795    5509    5771     892 
   Size:        755     548    1064     621     915     714     262    1846

  Fragments arranged by size: 

                1846    1064     915     755     714     621     548     262
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6725
 
SphI G_CATG'C

Cuts at:   3778    4278    3778 
   Size:        500    6225
 
SspI AAT'ATT

Cuts at:   1986    1986 
   Size:       6725
 
StuI AGG'CCT

Cuts at:    935    2817     935 
   Size:       1882    4843
 
StyI C'CwwG_G

Cuts at:    936    1992    3859    4213     936 
   Size:       1056    1867     354    3448

  Fragments arranged by size: 

                3448    1867    1056     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   4308    4441    4900    5109    4308 
   Size:        133     459     209    5924

  Fragments arranged by size: 

                5924     459     209     133
 
TatI w'GTAC_w

Cuts at:    142     645    1280    1438    1510    1562    1901    3119    3654 
   Size:        503     635     158      72      52     339    1218     535

Cuts at:   3654    6240     142 
   Size:       2586     627

  Fragments arranged by size: 

                2586    1218     635     627     535     503     339     158
                  72      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3012    3044    6242    6274    3012 
   Size:         32    3198      32    3463

  Fragments arranged by size: 

                3463    3198      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1600    4522    1600 
   Size:       2922    3803
 
XbaI T'CTAG_A

Cuts at:   3222    3756    3222 
   Size:        534    6191
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1999    4220    1999 
   Size:       2221    4504
 
XmaI C'CCGG_G

Cuts at:   3745    3745 
   Size:       6725
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AhdI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NsiI     NspI     PciI    PflMI     PmeI     PpiI    PpuMI
     PsiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII    AflII     AgeI     AloI     ApaI     AscI    AvrII     BaeI
    BbvCI     BcgI     BmtI    BsiWI   BssHII   BstEII     ClaI    CspCI
     FseI    FspAI     NcoI     NheI     NotI     NruI     PacI     PasI
     PfoI     PmlI    PshAI   PspOMI    PspXI    SacII    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SwaI     XhoI     XmnI     ZraI
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