Restriction map for pF1KA1287
 (Linear) (Six-base) MAPSORT of: KIAA1287.seq  Check: 9914  from: 1  to: 3612



KIAA1287.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     177    3612 
   Size:        177    3435
 
AccI GT'mk_AC

Cuts at:      0    2542    3195    3612 
   Size:       2542     653     417

  Fragments arranged by size: 

                2542     653     417
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     349     614    1232    1391    2491    2707    2867 
   Size:         28     321     265     618     159    1100     216     160

Cuts at:   2867    3612 
   Size:        745

  Fragments arranged by size: 

                1100     745     618     321     265     216     160     159
                  28
 
AfeI AGC'GCT

Cuts at:      0    2919    3612 
   Size:       2919     693
 
AflIII A'CryG_T

Cuts at:      0     606    2636    3442    3612 
   Size:        606    2030     806     170

  Fragments arranged by size: 

                2030     806     606     170
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1250    3612 
   Size:       1250    2362
 
AleI CACnn'nnGTG

Cuts at:      0    2308    3612 
   Size:       2308    1304
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3410    3612 
   Size:       3410     202
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2206    3612 
   Size:       2206    1406
 
ApaLI G'TGCA_C

Cuts at:      0     164    1157    3612 
   Size:        164     993    2455

  Fragments arranged by size: 

                2455     993     164
 
ApoI r'AATT_y

Cuts at:      0     475    1571    1800    2068    2403    2423    3612 
   Size:        475    1096     229     268     335      20    1189

  Fragments arranged by size: 

                1189    1096     475     335     268     229      20
 
BamHI G'GATC_C

Cuts at:      0    3514    3537    3612 
   Size:       3514      23      75

  Fragments arranged by size: 

                3514      75      23
 
BanI G'GyrC_C

Cuts at:      0     619    3612 
   Size:        619    2993
 
BanII G_rGCy'C

Cuts at:      0     195     563    1106    3612 
   Size:        195     368     543    2506

  Fragments arranged by size: 

                2506     543     368     195
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      34     482    1558    2005    2954    3439    3612 
   Size:         34     448    1076     447     949     485     173

  Fragments arranged by size: 

                1076     949     485     448     447     173      34
 
BclI T'GATC_A

Cuts at:      0    2589    3612 
   Size:       2589    1023
 
BfrBI ATG'CAT

Cuts at:      0    2785    3205    3612 
   Size:       2785     420     407

  Fragments arranged by size: 

                2785     420     407
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2478    3612 
   Size:       2478    1134
 
BlpI GC'TnA_GC

Cuts at:      0    1223    3035    3281    3612 
   Size:       1223    1812     246     331

  Fragments arranged by size: 

                1812    1223     331     246
 
Bme1580I G_kGCm'C

Cuts at:      0     168    1161    3612 
   Size:        168     993    2451

  Fragments arranged by size: 

                2451     993     168
 
BmgBI CAC'GTC

Cuts at:      0    3445    3612 
   Size:       3445     167
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2287    3612 
   Size:       2287    1325
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1271    3612 
   Size:       1271    2341
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248    1054    3612 
   Size:        248     806    2558

  Fragments arranged by size: 

                2558     806     248
 
Bpu10I CC'TnA_GC

Cuts at:      0     194     313    2798    3128    3612 
   Size:        194     119    2485     330     484

  Fragments arranged by size: 

                2485     484     330     194     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1560    2072    3172    3612 
   Size:        769     791     512    1100     440

  Fragments arranged by size: 

                1100     791     769     512     440
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     982    3327    3485    3612 
   Size:        982    2345     158     127

  Fragments arranged by size: 

                2345     982     158     127
 
BsaBI GATnn'nnATC

Cuts at:      0    1189    2062    3612 
   Size:       1189     873    1550

  Fragments arranged by size: 

                1550    1189     873
 
BsaHI Gr'CG_yC

Cuts at:      0    1412    3612 
   Size:       1412    2200
 
BsaWI w'CCGG_w

Cuts at:      0     938    2754    3612 
   Size:        938    1816     858

  Fragments arranged by size: 

                1816     938     858
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1740    1770    2120    2150    2736    2766    2904    2934 
   Size:       1740      30     350      30     586      30     138      30

Cuts at:   2934    3612 
   Size:        678

  Fragments arranged by size: 

                1740     678     586     350     138      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     344    2866    3612 
   Size:        344    2522     746

  Fragments arranged by size: 

                2522     746     344
 
BseYI C'CCAG_C

Cuts at:      0     188    1270    3612 
   Size:        188    1082    2342

  Fragments arranged by size: 

                2342    1082     188
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2828    3612 
   Size:       2828     784
 
BsiEI CG_ry'CG

Cuts at:      0    3086    3612 
   Size:       3086     526
 
BsiHKAI G_wGCw'C

Cuts at:      0     168     563    1161    1955    2682    3612 
   Size:        168     395     598     794     727     930

  Fragments arranged by size: 

                 930     794     727     598     395     168
 
BsmI GAATG_Cn'

Cuts at:      0    1046    2118    2231    3612 
   Size:       1046    1072     113    1381

  Fragments arranged by size: 

                1381    1072    1046     113
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1419    2140    3612 
   Size:       1419     721    1472

  Fragments arranged by size: 

                1472    1419     721
 
Bsp1286I G_dGCh'C

Cuts at:      0     168     195     563    1106    1161    1955    2682    3612 
   Size:        168      27     368     543      55     794     727     930

  Fragments arranged by size: 

                 930     794     727     543     368     168      55      27
            
 
BspEI T'CCGG_A

Cuts at:      0     938    2754    3612 
   Size:        938    1816     858

  Fragments arranged by size: 

                1816     938     858
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     177    1575    3079    3612 
   Size:        177    1398    1504     533

  Fragments arranged by size: 

                1504    1398     533     177
 
BsrBI CCG'CTC

Cuts at:      0    2797    3612 
   Size:       2797     815
 
BsrDI GCAATG_nn'

Cuts at:      0      80    3014    3029    3612 
   Size:         80    2934      15     583

  Fragments arranged by size: 

                2934     583      80      15
 
BsrGI T'GTAC_A

Cuts at:      0      34    1793    3011    3546    3612 
   Size:         34    1759    1218     535      66

  Fragments arranged by size: 

                1759    1218     535      66      34
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     163    1233    3612 
   Size:        163    1070    2379

  Fragments arranged by size: 

                2379    1070     163
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3476    3612 
   Size:       3476     136
 
BstYI r'GATC_y

Cuts at:      0    2565    3021    3514    3537    3612 
   Size:       2565     456     493      23      75

  Fragments arranged by size: 

                2565     493     456      75      23
 
BstZ17I GTA'TAC

Cuts at:      0    2543    3196    3612 
   Size:       2543     653     416

  Fragments arranged by size: 

                2543     653     416
 
Bsu36I CC'TnA_GG

Cuts at:      0    2581    3612 
   Size:       2581    1031
 
BtgI C'CryG_G

Cuts at:      0     266    2416    3612 
   Size:        266    2150    1196

  Fragments arranged by size: 

                2150    1196     266
 
BtsI GCAGTG_nn'

Cuts at:      0     342    3612 
   Size:        342    3270
 
DraIII CAC_nnn'GTG

Cuts at:      0     163    1710    3223    3612 
   Size:        163    1547    1513     389

  Fragments arranged by size: 

                1547    1513     389     163
 
EaeI y'GGCC_r

Cuts at:      0     644    2792    3612 
   Size:        644    2148     820

  Fragments arranged by size: 

                2148     820     644
 
EarI CTCTTCn'nnn_

Cuts at:      0    1130    1970    1988    2843    3612 
   Size:       1130     840      18     855     769

  Fragments arranged by size: 

                1130     855     840     769      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1900    3612 
   Size:       1900    1712
 
EcoICRI GAG'CTC

Cuts at:      0     561    3612 
   Size:        561    3051
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     248     349     614    1054    1232    1391    2491 
   Size:         28     220     101     265     440     178     159    1100

Cuts at:   2491    2707    2867    3612 
   Size:        216     160     745

  Fragments arranged by size: 

                1100     745     440     265     220     216     178     160
                 159     101      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1542    3612 
   Size:       1542    2070
 
EcoO109I rG'GnC_Cy

Cuts at:      0     681     780    1611    3612 
   Size:        681      99     831    2001

  Fragments arranged by size: 

                2001     831     681      99
 
EcoRI G'AATT_C

Cuts at:      0    2403    2423    3612 
   Size:       2403      20    1189

  Fragments arranged by size: 

                2403    1189      20
 
EcoRV GAT'ATC

Cuts at:      0    2650    3612 
   Size:       2650     962
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     736    1575    1999    2689    3612 
   Size:        736     839     424     690     923

  Fragments arranged by size: 

                 923     839     736     690     424
 
HaeII r_GCGC'y

Cuts at:      0    2921    3612 
   Size:       2921     691
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     332     364     834     866    1236    1268    2550    2553 
   Size:        332      32     470      32     370      32    1282       3

Cuts at:   2553    2582    2585    3349    3381    3612 
   Size:         29       3     764      32     231

  Fragments arranged by size: 

                1282     764     470     370     332     231      32      32
                  32      32      29       3       3
 
HincII GTy'rAC

Cuts at:      0     243    2378    3263    3612 
   Size:        243    2135     885     349

  Fragments arranged by size: 

                2135     885     349     243
 
HindIII A'AGCT_T

Cuts at:      0     596     866    3037    3612 
   Size:        596     270    2171     575

  Fragments arranged by size: 

                2171     596     575     270
 
HpaI GTT'AAC

Cuts at:      0    2378    3263    3612 
   Size:       2378     885     349

  Fragments arranged by size: 

                2378     885     349
 
MfeI C'AATT_G

Cuts at:      0    2726    3612 
   Size:       2726     886
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     491    1351    2718    3612 
   Size:        491     860    1367     894

  Fragments arranged by size: 

                1367     894     860     491
 
MscI TGG'CCA

Cuts at:      0     646    3612 
   Size:        646    2966
 
MslI CAynn'nnrTG

Cuts at:      0     802    2160    2308    3612 
   Size:        802    1358     148    1304

  Fragments arranged by size: 

                1358    1304     802     148
 
MspA1I CmG'CkG

Cuts at:      0    1270    1350    1740    2287    2812    3612 
   Size:       1270      80     390     547     525     800

  Fragments arranged by size: 

                1270     800     547     525     390      80
 
NdeI CA'TA_TG

Cuts at:      0    2364    3612 
   Size:       2364    1248
 
NsiI A_TGCA'T

Cuts at:      0    2787    3207    3612 
   Size:       2787     420     405

  Fragments arranged by size: 

                2787     420     405
 
NspI r_CATG'y

Cuts at:      0     610    1144    2640    3612 
   Size:        610     534    1496     972

  Fragments arranged by size: 

                1496     972     610     534
 
PciI A'CATG_T

Cuts at:      0     606    2636    3612 
   Size:        606    2030     976

  Fragments arranged by size: 

                2030     976     606
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     829    3612 
   Size:        829    2783
 
PpuMI rG'GwC_Cy

Cuts at:      0    1611    3612 
   Size:       1611    2001
 
PsiI TTA'TAA

Cuts at:      0    3506    3612 
   Size:       3506     106
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1853    1885    3612 
   Size:       1853      32    1727

  Fragments arranged by size: 

                1853    1727      32
 
PstI C_TGCA'G

Cuts at:      0     534    1238    2099    3612 
   Size:        534     704     861    1513

  Fragments arranged by size: 

                1513     861     704     534
 
PvuII CAG'CTG

Cuts at:      0    1270    1350    1740    2287    2812    3612 
   Size:       1270      80     390     547     525     800

  Fragments arranged by size: 

                1270     800     547     525     390      80
 
SacI G_AGCT'C

Cuts at:      0     563    3612 
   Size:        563    3049
 
SanDI GG'GwC_CC

Cuts at:      0    1611    3612 
   Size:       1611    2001
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1130    1988    3612 
   Size:       1130     858    1624

  Fragments arranged by size: 

                1624    1130     858
 
ScaI AGT'ACT

Cuts at:      0    1404    3612 
   Size:       1404    2208
 
SfcI C'TryA_G

Cuts at:      0     530     578    1234    2095    3612 
   Size:        530      48     656     861    1517

  Fragments arranged by size: 

                1517     861     656     530      48
 
SmlI C'TyrA_G

Cuts at:      0     784    1539    2087    3151    3612 
   Size:        784     755     548    1064     461

  Fragments arranged by size: 

                1064     784     755     548     461
 
SspI AAT'ATT

Cuts at:      0    1878    3612 
   Size:       1878    1734
 
StuI AGG'CCT

Cuts at:      0     827    2709    3612 
   Size:        827    1882     903

  Fragments arranged by size: 

                1882     903     827
 
StyI C'CwwG_G

Cuts at:      0     828    1884    3612 
   Size:        828    1056    1728

  Fragments arranged by size: 

                1728    1056     828
 
TatI w'GTAC_w

Cuts at:      0      34     537    1172    1330    1402    1454    1793    3011 
   Size:         34     503     635     158      72      52     339    1218

Cuts at:   3011    3546    3612 
   Size:        535      66

  Fragments arranged by size: 

                1218     635     535     503     339     158      72      66
                  52      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2904    2936    3612 
   Size:       2904      32     676

  Fragments arranged by size: 

                2904     676      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1492    3612 
   Size:       1492    2120
 
XbaI T'CTAG_A

Cuts at:      0    3114    3612 
   Size:       3114     498
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1891    3612 
   Size:       1891    1721
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
    AlwNI    ApaLI     ApoI    BamHI     BanI    BanII     BbsI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrGI   BstAPI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I
     NdeI     NsiI     NspI     PciI    PflMI    PpuMI     PsiI     PsrI
     PstI    PvuII     SacI    SanDI     SapI     ScaI     SfcI     SmlI
     SspI     StuI     StyI     TatI     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AloI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI    BglII     BmtI    BsaAI    BsiWI    BspHI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     FseI     FspI    FspAI     KasI     KpnI     MluI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PvuI
    RsrII    SacII     SalI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII
     XhoI     XmaI     XmnI     ZraI
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