Restriction map for pF1KA0246
##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AclI        AcsI        AhdI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
BsaBI       BsiWI       BspDI       BspHI       Bst1107I    
BstZ17I     ClaI        DraI        Eam1105I    Eco47III    
EcoRI       EcoRV       HincII      HindII      HpaI        
MamI        MluI        NotI        NruI        PacI        
PaeR7I      PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        SalI        SbfI        ScaI        
SgrAI       SpeI        Sse8387I    SspI        SwaI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        5748  GACGTC               GACGT^C
Acc65I       1724  GGTACC               G^GTACC
AflII        3022  CTTAAG               C^TTAAG
Asp718I      1724  GGTACC               G^GTACC
AspI         5113  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4908  TGCGCA               TGC^GCA
AvrII        4261  CCTAGG               C^CTAGG
BbeI         7407  GGCGCC               GGCGC^C
BclI          576  TGATCA               T^GATCA
BfrI         3022  CTTAAG               C^TTAAG
BlnI         4261  CCTAGG               C^CTAGG
BsmBI        1209  CGTCTC               CGTCTC(1/5)
Bsp1407I     6265  TGTACA               T^GTACA
BsrGI        6265  TGTACA               T^GTACA
BssHII       5059  GCGCGC               G^CGCGC
BstBI        5447  TTCGAA               TT^CGAA
BstEII        999  GGTGACC              G^GT[ACGT]ACC
BstPI         999  GGTGACC              G^GT[ACGT]ACC
CpoI         2603  CGGACCG              CG^G[AT]CCG
EcoO65I       999  GGTGACC              G^GT[ACGT]ACC
FbaI          576  TGATCA               T^GATCA
FseI         5238  GGCCGGCC             GGCCGG^CC
FspI         4908  TGCGCA               TGC^GCA
HindIII      5906  AAGCTT               A^AGCTT
KasI         7407  GGCGCC               G^GCGCC
KpnI         1724  GGTACC               GGTAC^C
NarI         7407  GGCGCC               GG^CGCC
NdeI         4658  CATATG               CA^TATG
NheI         5370  GCTAGC               G^CTAGC
NspV         5447  TTCGAA               TT^CGAA
PflFI        5113  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsrII        2603  CGGACCG              CG^G[AT]CCG
SapI         3876  GAAGAGC              GCTCTTC(1/4)
SfiI         7281  GGCCCCTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         7407  GGCGCC               GGC^GCC
SfuI         5447  TTCGAA               TT^CGAA
SnaBI        6496  TACGTA               TAC^GTA
SspBI        6265  TGTACA               T^GTACA
Tth111I      5113  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII       4604  TCCGGA               T^CCGGA
             5325  TCCGGA              
AgeI          461  ACCGGT               A^CCGGT
              980  ACCGGT              
BciVI         556  GGATAC               GTATCC(6/5)
             4333  GGATAC              
BglII        4863  AGATCT               A^GATCT
             5213  AGATCT              
BlpI          431  GCTCAGC              GC^T[ACGT]AGC
             3099  GCTGAGC             
Bpu1102I      431  GCTCAGC              GC^T[ACGT]AGC
             3099  GCTGAGC             
BseAI        4604  TCCGGA               T^CCGGA
             5325  TCCGGA              
BspEI        4604  TCCGGA               T^CCGGA
             5325  TCCGGA              
BspLU11I     3928  ACATGT               A^CATGT
             4696  ACATGT              
CelII         431  GCTCAGC              GC^T[ACGT]AGC
             3099  GCTGAGC             
DrdI         3052  GACCGCCGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6509  GACTGCAGTGTC        
EagI         1668  CGGCCG               C^GGCCG
             4301  CGGCCG              
EclXI        1668  CGGCCG               C^GGCCG
             4301  CGGCCG              
Eco52I       1668  CGGCCG               C^GGCCG
             4301  CGGCCG              
EcoT22I      1238  ATGCAT               ATGCA^T
             5455  ATGCAT              
KspI          426  CCGCGG               CCGC^GG
             6420  CCGCGG              
MfeI         2232  CAATTG               C^AATTG
             5844  CAATTG              
MroI         4604  TCCGGA               T^CCGGA
             5325  TCCGGA              
MunI         2232  CAATTG               C^AATTG
             5844  CAATTG              
NsiI         1238  ATGCAT               ATGCA^T
             5455  ATGCAT              
PinAI         461  ACCGGT               A^CCGGT
              980  ACCGGT              
SacII         426  CCGCGG               CCGC^GG
             6420  CCGCGG              
SexAI        1799  ACCAGGT              A^CC[AT]GGT
             3149  ACCTGGT             


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
AocI        ApaI        ApaLI       AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com