Restriction map for pF1KA0038
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflIII      AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaWI       BseAI       BseRI       BsgI        BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MslI        MunI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcyI          597  GACGCC               G[GA]^CG[CT]C
AfaI          208  GTAC                 GT^AC
AflII         283  CTTAAG               C^TTAAG
Alw44I        308  GTGCAC               G^TGCAC
ApaLI         308  GTGCAC               G^TGCAC
Asp700I       661  GAAGTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI           78  TGGCCA               TGG^CCA
BamHI         514  GGATCC               G^GATCC
BanI          643  GGTGCC               G^G[CT][GA]CC
BanII         115  GAGCCC               G[GA]GC[CT]^C
BcgI          168  CGACATAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          283  CTTAAG               C^TTAAG
BpmI          571  CTCCAG               CTGGAG(16/14)
BsaAI         133  TACGTA               [CT]AC^GT[GA]
BsaHI         597  GACGCC               G[GA]^CG[CT]C
BsaI          561  GAGACC               GGTCTC(1/5)
BsmFI          60  GTCCC                GGGAC(10/14)
BsrDI         342  CATTGC               GCAATG(2/0)
BsrFI         458  ACCGGC               [GA]^CCGG[CT]
BstF5I        417  GGATG                GGATG(2/0)
Cfr10I        458  ACCGGC               [GA]^CCGG[CT]
DraI          241  TTTAAA               TTT^AAA
DraIII        329  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           78  TGGCCA               [CT]^GGCC[GA]
EarI          544  GAAGAG               CTCTTC(1/4)
EcoRI         262  GAATTC               G^AATTC
FauI           62  CCCGC                CCCGC(4/6)
FokI          417  GGATG                GGATG(9/13)
HapII         459  CCGG                 C^CGG
HgaI          597  GACGC                GACGC(5/10)
Hin1I         597  GACGCC               G[GA]^CG[CT]C
HpaII         459  CCGG                 C^CGG
Ksp632I       544  GAAGAG               CTCTTC(1/4)
KspI           63  CCGCGG               CCGC^GG
MaeII         134  ACGT                 A^CGT
MaeIII        327  GTCAC                ^GT[ACGT]AC
MluNI          78  TGGCCA               TGG^CCA
MscI           78  TGGCCA               TGG^CCA
MspA1I         63  CCGCGG               C[AC]G^C[GT]G
MspI          459  CCGG                 C^CGG
NruI          593  TCGCGA               TCG^CGA
PleI          569  GACTC                GAGTC(4/5)
PpuMI          58  GGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          208  GTAC                 GT^AC
SacII          63  CCGCGG               CCGC^GG
SfaNI         175  GATGC                GCATC(5/9)
SfcI           33  CTACAG               C^T[GA][CT]AG
SmlI          283  CTTAAG               C^T[CT][GA]AG
SnaBI         133  TACGTA               TAC^GTA
StuI          649  AGGCCT               AGG^CCT
Tsp45I        327  GTCAC                ^GT[GC]AC
XmnI          661  GAAGTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII          64  CGCG                 CG^CG
              594  CGCG                
AcsI          238  AAATTT               [GA]^AATT[CT]
              262  GAATTC              
AluI           40  AGCT                 AG^CT
              575  AGCT                
ApoI          238  AAATTT               [GA]^AATT[CT]
              262  GAATTC              
AspHI         308  GTGCAC               G[AT]GC[AT]^C
              554  GAGCAC              
AvaII          59  GGTCC                G^G[AT]CC
              394  GGACC               
BbvI           38  GCAGC                GCAGC(8/12)
              482  GCAGC               
BfaI          214  CTAG                 C^TAG
              653  CTAG                
BsiHKAI       308  GTGCAC               G[AT]GC[AT]^C
              554  GAGCAC              
BsmAI         221  GAGAC                GTCTC(1/5)
              561  GAGAC               
BssKI         451  CCAGG                ^CC[ACGT]GG
              647  CCAGG               
BstNI         451  CCAGG                CC^[AT]GG
              647  CCAGG               
BstUI          64  CGCG                 CG^CG
              594  CGCG                
BstYI         189  GGATCT               [GA]^GATC[CT]
              514  GGATCC              
DdeI          193  CTCAG                C^T[ACGT]AG
              426  CTTAG               
DraII          58  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              468  AGGCCCC             
EcoO109I       58  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              468  AGGCCCC             
EcoRII        451  CCAGG                ^CC[AT]GG
              647  CCAGG               
EcoT14I        81  CCATGG               C^C[AT][AT]GG
              476  CCATGG              
MaeI          214  CTAG                 C^TAG
              653  CTAG                
MboII         544  GAAGA                GAAGA(8/7)
              635  TCTTC               
MflI          189  GGATCT               [GA]^GATC[CT]
              514  GGATCC              
MvaI          451  CCAGG                CC^[AT]GG
              647  CCAGG               
MvnI           64  CGCG                 CG^CG
              594  CGCG                
NcoI           81  CCATGG               C^CATGG
              476  CCATGG              
PvuI           24  CGATCG               CGAT^CG
              321  CGATCG              
ScrFI         451  CCAGG                CC^[ACGT]GG
              647  CCAGG               
StyI           81  CCATGG               C^C[AT][AT]GG
              476  CCATGG              
TseI           38  GCAGC                G^C[AT]GC
              482  GCAGC               
Tsp509I       239  AATT                 ^AATT
              263  AATT                
TspRI          69  CAGTG                CA[GC]TG[ACGT][ACGT]^
              311  CACTG               
XhoII         189  GGATCT               [GA]^GATC[CT]
              514  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AlwI        BmyI        BsaJI       BsiEI       
BsiYI       BslI        Bsp1286I    Cfr13I      DpnII       
DsaI        Fnu4HI      HaeIII      HinfI       ItaI        
MboI        MnlI        MseI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      TaqI        
TfiI        Tru9I       TthHB8I     
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