Restriction map for pF1KSDB0014
##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AgeI        
AocI        AscI        AseI        AsnI        Asp718I     
AvrII       BciVI       BlnI        BsaWI       BseAI       
BspDI       BspEI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        DraI        DraIII      
Eco81I      EcoRV       EcoT22I     FseI        HindIII     
HpaI        KpnI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        SalI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII        2341  GACGTC               GACGT^C
AflII        5368  CTTAAG               C^TTAAG
Alw44I        513  GTGCAC               G^TGCAC
ApaLI         513  GTGCAC               G^TGCAC
Asp700I      4014  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         3817  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         267  CACGTG               CAC^GTG
BfrI         5368  CTTAAG               C^TTAAG
BlpI         3972  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3972  GCTGAGC              GC^T[ACGT]AGC
BsaBI          19  GATAAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          292  CTGCAC               GTGCAG(16/14)
BsiWI         575  CGTACG               C^GTACG
Bsp1407I      410  TGTACA               T^GTACA
BspHI        2225  TCATGA               T^CATGA
BspLU11I      950  ACATGT               A^CATGT
BsrGI         410  TGTACA               T^GTACA
CelII        3972  GCTGAGC              GC^T[ACGT]AGC
CpoI         5515  CGGACCG              CG^G[AT]CCG
EagI         5717  CGGCCG               C^GGCCG
EclXI        5717  CGGCCG               C^GGCCG
Eco52I       5717  CGGCCG               C^GGCCG
EcoRI        4014  GAATTC               G^AATTC
HincII       4678  GTCAAC               GT[CT]^[GA]AC
HindII       4678  GTCAAC               GT[CT]^[GA]AC
KspI         5072  CCGCGG               CCGC^GG
MamI           19  GATAAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI         5369  TTAA                 T^TAA
PflFI        3817  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         267  CACGTG               CAC^GTG
PmlI          267  CACGTG               CAC^GTG
RcaI         2225  TCATGA               T^CATGA
RsrII        5515  CGGACCG              CG^G[AT]CCG
SacII        5072  CCGCGG               CCGC^GG
SexAI        1865  ACCAGGT              A^CC[AT]GGT
SphI         4949  GCATGC               GCATG^C
SspBI         410  TGTACA               T^GTACA
Tru9I        5369  TTAA                 T^TAA
Tth111I      3817  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         4014  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          709  AAATTC               [GA]^AATT[CT]
             4014  GAATTC              
AflIII        918  ACACGT               A^C[GA][CT]GT
              950  ACATGT              
Aor51HI      2786  AGCGCT               AGC^GCT
             4811  AGCGCT              
ApoI          709  AAATTC               [GA]^AATT[CT]
             4014  GAATTC              
AviII        1292  TGCGCA               TGC^GCA
             3800  TGCGCA              
BamHI        1346  GGATCC               G^GATCC
             1767  GGATCC              
BbeI         1330  GGCGCC               GGCGC^C
             5812  GGCGCC              
BcgI           72  CGACTGGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2679  CGAGCTGTGTGC        
BclI         3083  TGATCA               T^GATCA
             5240  TGATCA              
BsaAI         267  CACGTG               [CT]AC^GT[GA]
              577  TACGTG              
BsmBI        2614  GAGACG               CGTCTC(1/5)
             5713  GAGACG              
BsmI         5331  GAATGC               GAATGC(1/-1)
             1568  GCATTC              
BsrDI        1356  CATTGC               GCAATG(2/0)
             3079  GCAATG              
BstEII       2898  GGTGACC              G^GT[ACGT]ACC
             3822  GGTCACC             
BstPI        2898  GGTGACC              G^GT[ACGT]ACC
             3822  GGTCACC             
DrdI         1813  GACAAGTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2616  GACGCTGCAGTC        
Eco47III     2786  AGCGCT               AGC^GCT
             4811  AGCGCT              
EcoO65I      2898  GGTGACC              G^GT[ACGT]ACC
             3822  GGTCACC             
FbaI         3083  TGATCA               T^GATCA
             5240  TGATCA              
FspI         1292  TGCGCA               TGC^GCA
             3800  TGCGCA              
KasI         1330  GGCGCC               G^GCGCC
             5812  GGCGCC              
NarI         1330  GGCGCC               GG^CGCC
             5812  GGCGCC              
NcoI          965  CCATGG               C^CATGG
             4463  CCATGG              
SfoI         1330  GGCGCC               GGC^GCC
             5812  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApaI        AspEI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eam1105I    
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PaeR7I      PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      SbfI        ScrFI       
SfaNI       SfcI        SfiI        SmaI        SmlI        
Sse8387I    StuI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoI        XhoII       XmaCI       
XmaI        
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