Restriction map for pF1KSDB0014
 (Linear) (Six-base) MAPSORT of: KIBB0014.seq  Check: 4866  from: 1  to: 5880



KIBB0014.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2505    2807    3825    3987    4587    5538    5880 
   Size:       2505     302    1018     162     600     951     342

  Fragments arranged by size: 

                2505    1018     951     600     342     302     162
 
AatII G_ACGT'C

Cuts at:      0    2345    5880 
   Size:       2345    3535
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     883    1024    1705    2350    2479    3535    4876 
   Size:        413     470     141     681     645     129    1056    1341

Cuts at:   4876    5044    5275    5551    5635    5880 
   Size:        168     231     276      84     245

  Fragments arranged by size: 

                1341    1056     681     645     470     413     276     245
                 231     168     141     129      84
 
AfeI AGC'GCT

Cuts at:      0    2788    4813    5880 
   Size:       2788    2025    1067

  Fragments arranged by size: 

                2788    2025    1067
 
AflII C'TTAA_G

Cuts at:      0    5368    5880 
   Size:       5368     512
 
AflIII A'CryG_T

Cuts at:      0     918     950    5880 
   Size:        918      32    4930

  Fragments arranged by size: 

                4930     918      32
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1086    3360    3882    5880 
   Size:       1086    2274     522    1998

  Fragments arranged by size: 

                2274    1998    1086     522
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2603    5524    5880 
   Size:       2603    2921     356

  Fragments arranged by size: 

                2921    2603     356
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     803     835    1582    1614    5880 
   Size:        803      32     747      32    4266

  Fragments arranged by size: 

                4266     803     747      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     570     633    2799    2883    3135    3663    3768    3945 
   Size:        570      63    2166      84     252     528     105     177

Cuts at:   3945    3972    4263    4347    5346    5880 
   Size:         27     291      84     999     534

  Fragments arranged by size: 

                2166     999     570     534     528     291     252     177
                 105      84      84      63      27
 
ApaI G_GGCC'C

Cuts at:      0    2712    4452    4587    5880 
   Size:       2712    1740     135    1293

  Fragments arranged by size: 

                2712    1740    1293     135
 
ApaLI G'TGCA_C

Cuts at:      0     513    5880 
   Size:        513    5367
 
ApoI r'AATT_y

Cuts at:      0     709    4014    5880 
   Size:        709    3305    1866

  Fragments arranged by size: 

                3305    1866     709
 
AvaI C'yCGr_G

Cuts at:      0     931    1563    1897    2092    3643    4412    4445    5434 
   Size:        931     632     334     195    1551     769      33     989

Cuts at:   5434    5880 
   Size:        446

  Fragments arranged by size: 

                1551     989     931     769     632     446     334     195
                  33
 
BamHI G'GATC_C

Cuts at:      0    1346    1767    5880 
   Size:       1346     421    4113

  Fragments arranged by size: 

                4113    1346     421
 
BanI G'GyrC_C

Cuts at:      0      99    1330    1651    4133    5812    5880 
   Size:         99    1231     321    2482    1679      68

  Fragments arranged by size: 

                2482    1679    1231     321      99      68
 
BanII G_rGCy'C

Cuts at:      0     266     326    2246    2705    2712    3200    3221    3254 
   Size:        266      60    1920     459       7     488      21      33

Cuts at:   3254    3482    4452    4534    4587    5154    5880 
   Size:        228     970      82      53     567     726

  Fragments arranged by size: 

                1920     970     726     567     488     459     266     228
                  82      60      53      33      21       7
 
BbeI G_GCGC'C

Cuts at:      0    1334    5816    5880 
   Size:       1334    4482      64

  Fragments arranged by size: 

                4482    1334      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     871    1050    2130    2274    2999    3148    3359    3526 
   Size:        871     179    1080     144     725     149     211     167

Cuts at:   3526    4243    4366    4618    5880 
   Size:        717     123     252    1262

  Fragments arranged by size: 

                1262    1080     871     725     717     252     211     179
                 167     149     144     123
 
BbvCI CC'TCA_GC

Cuts at:      0    1981    2696    3178    4148    4400    5861    5880 
   Size:       1981     715     482     970     252    1461      19

  Fragments arranged by size: 

                1981    1461     970     715     482     252      19
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      61      95    2668    2702    5880 
   Size:         61      34    2573      34    3178

  Fragments arranged by size: 

                3178    2573      61      34      34
 
BclI T'GATC_A

Cuts at:      0    3083    5240    5880 
   Size:       3083    2157     640

  Fragments arranged by size: 

                3083    2157     640
 
BglI GCCn_nnn'nGGC

Cuts at:      0      76    1243    1565    2117    3265    4096    4696    5437 
   Size:         76    1167     322     552    1148     831     600     741

Cuts at:   5437    5599    5662    5880 
   Size:        162      63     218

  Fragments arranged by size: 

                1167    1148     831     741     600     552     322     218
                 162      76      63
 
BglII A'GATC_T

Cuts at:      0    1370    2744    3335    5880 
   Size:       1370    1374     591    2545

  Fragments arranged by size: 

                2545    1374    1370     591
 
BlpI GC'TnA_GC

Cuts at:      0    3973    5880 
   Size:       3973    1907
 
Bme1580I G_kGCm'C

Cuts at:      0     517    1654    2712    4452    4587    5398    5792    5880 
   Size:        517    1137    1058    1740     135     811     394      88

  Fragments arranged by size: 

                1740    1137    1058     811     517     394     135      88
            
 
BmgBI CAC'GTC

Cuts at:      0     921    5880 
   Size:        921    4959
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      84    4695    5880 
   Size:         84    4611    1185

  Fragments arranged by size: 

                4611    1185      84
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1183    3183    5880 
   Size:       1183    2000    2697

  Fragments arranged by size: 

                2697    2000    1183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     640     673     872    1480    2260    2332    2419 
   Size:        548      92      33     199     608     780      72      87

Cuts at:   2419    2650    2875    2911    2947    2968    3157    3178    3184 
   Size:        231     225      36      36      21     189      21       6

Cuts at:   3184    3238    3331    3379    3547    3634    3685    3937    4054 
   Size:         54      93      48     168      87      51     252     117

Cuts at:   4054    4084    4153    4201    4252    4336    4474    4504    4588 
   Size:         30      69      48      51      84     138      30      84

Cuts at:   4588    4651    4813    4864    4885    5200    5221    5233    5323 
   Size:         63     162      51      21     315      21      12      90

Cuts at:   5323    5476    5656    5719    5880 
   Size:        153     180      63     161

  Fragments arranged by size: 

                 780     608     548     315     252     231     225     199
                 189     180     168     162     161     153     138     117
                  93      92      90      87      87      84      84      72
                  69      63      63      54      51      51      51      48
                  48      36      36      33      30      30      21      21
                  21      21      12       6
 
Bpu10I CC'TnA_GC

Cuts at:      0    1981    2696    3178    3481    3979    4148    4189    4400 
   Size:       1981     715     482     303     498     169      41     211

Cuts at:   4400    5761    5861    5880 
   Size:       1361     100      19

  Fragments arranged by size: 

                1981    1361     715     498     482     303     211     169
                 100      41      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     286    2863    3046    3205    3292    3976    5608 
   Size:        121     165    2577     183     159      87     684    1632

Cuts at:   5608    5880 
   Size:        272

  Fragments arranged by size: 

                2577    1632     684     272     183     165     159     121
                  87
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     955    1879    2318    4417    5470    5880 
   Size:        955     924     439    2099    1053     410

  Fragments arranged by size: 

                2099    1053     955     924     439     410
 
BsaAI yAC'GTr

Cuts at:      0     269     579    5880 
   Size:        269     310    5301

  Fragments arranged by size: 

                5301     310     269
 
BsaBI GATnn'nnATC

Cuts at:      0      23    5880 
   Size:         23    5857
 
BsaHI Gr'CG_yC

Cuts at:      0     583    1087    1331    2342    5175    5705    5813    5880 
   Size:        583     504     244    1011    2833     530     108      67

  Fragments arranged by size: 

                2833    1011     583     530     504     244     108      67
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     679     709    1582    1612    3469    3499    3782    3812 
   Size:        679      30     873      30    1857      30     283      30

Cuts at:   3812    5404    5434    5880 
   Size:       1592      30     446

  Fragments arranged by size: 

                1857    1592     873     679     446     283      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     684    1015    1483    1603    2329    2332    2545 
   Size:        157     527     331     468     120     726       3     213

Cuts at:   2545    2548    2566    2569    2713    2788    2791    2794    2797 
   Size:          3      18       3     144      75       3       3       3

Cuts at:   2797    2860    2872    2875    2878    2944    2947    3046    3049 
   Size:         63      12       3       3      66       3      99       3

Cuts at:   3049    3130    3259    3262    3544    3547    3610    3970    4051 
   Size:         81     129       3     282       3      63     360      81

Cuts at:   4051    4054    4057    4060    4165    4219    4369    4399    4618 
   Size:          3       3       3     105      54     150      30     219

Cuts at:   4618    4756    4999    5179    5218    5221    5230    5233    5473 
   Size:        138     243     180      39       3       9       3     240

Cuts at:   5473    5653    5665    5880 
   Size:        180      12     215

  Fragments arranged by size: 

                 726     527     468     360     331     282     243     240
                 219     215     213     180     180     157     150     144
                 138     129     120     105      99      81      81      75
                  66      63      63      54      39      30      18      12
                  12       9       3       3       3       3       3       3
                   3       3       3       3       3       3       3       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    3470    3923    5096    5195    5657    5880 
   Size:       3470     453    1173      99     462     223

  Fragments arranged by size: 

                3470    1173     462     453     223      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277    5880 
   Size:        277    5603
 
BsiEI CG_ry'CG

Cuts at:      0    4395    5250    5720    5880 
   Size:       4395     855     470     160

  Fragments arranged by size: 

                4395     855     470     160
 
BsiHKAI G_wGCw'C

Cuts at:      0     266     517     860    2225    2246    2705    3200    3221 
   Size:        266     251     343    1365      21     459     495      21

Cuts at:   3221    3254    3482    3734    3985    4534    5880 
   Size:         33     228     252     251     549    1346

  Fragments arranged by size: 

                1365    1346     549     495     459     343     266     252
                 251     251     228      33      21      21
 
BsiWI C'GTAC_G

Cuts at:      0     575    5880 
   Size:        575    5305
 
BsmI GAATG_Cn'

Cuts at:      0    1568    5337    5880 
   Size:       1568    3769     543

  Fragments arranged by size: 

                3769    1568     543
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2608    5707    5880 
   Size:       2608    3099     173

  Fragments arranged by size: 

                3099    2608     173
 
Bsp1286I G_dGCh'C

Cuts at:      0     266     326     517     860    1654    2225    2246    2705 
   Size:        266      60     191     343     794     571      21     459

Cuts at:   2705    2712    3200    3221    3254    3482    3734    3985    4452 
   Size:          7     488      21      33     228     252     251     467

Cuts at:   4452    4534    4587    5154    5398    5792    5880 
   Size:         82      53     567     244     394      88

  Fragments arranged by size: 

                 794     571     567     488     467     459     394     343
                 266     252     251     244     228     191      88      82
                  60      53      33      21      21       7
 
BspHI T'CATG_A

Cuts at:      0    2225    5880 
   Size:       2225    3655
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     386    1529    2505    2807    3762    3825    3987    4280 
   Size:        386    1143     976     302     955      63     162     293

Cuts at:   4280    4587    4691    4724    5276    5538    5783    5880 
   Size:        307     104      33     552     262     245      97

  Fragments arranged by size: 

                1143     976     955     552     386     307     302     293
                 262     245     162     104      97      63      33
 
BsrBI CCG'CTC

Cuts at:      0     624    1071    1269    3675    4488    4710    5106    5562 
   Size:        624     447     198    2406     813     222     396     456

Cuts at:   5562    5880 
   Size:        318

  Fragments arranged by size: 

                2406     813     624     456     447     396     318     222
                 198
 
BsrDI GCAATG_nn'

Cuts at:      0    1355    3086    5880 
   Size:       1355    1731    2794

  Fragments arranged by size: 

                2794    1731    1355
 
BsrFI r'CCGG_y

Cuts at:      0    1360    1720    1873    2038    2293    2522    3962    5249 
   Size:       1360     360     153     165     255     229    1440    1287

Cuts at:   5249    5880 
   Size:        631

  Fragments arranged by size: 

                1440    1360    1287     631     360     255     229     165
                 153
 
BsrGI T'GTAC_A

Cuts at:      0     410    5880 
   Size:        410    5470
 
BssSI C'ACGA_G

Cuts at:      0     430     785    2026    2059    3547    4201    4561    4979 
   Size:        430     355    1241      33    1488     654     360     418

Cuts at:   4979    5880 
   Size:        901

  Fragments arranged by size: 

                1488    1241     901     654     430     418     360     355
                  33
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     435     633    2061    2883    3768    5880 
   Size:        435     198    1428     822     885    2112

  Fragments arranged by size: 

                2112    1428     885     822     435     198
 
BstEII G'GTnAC_C

Cuts at:      0    2898    3822    5880 
   Size:       2898     924    2058

  Fragments arranged by size: 

                2898    2058     924
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      90    1971    2133    5880 
   Size:         90    1881     162    3747

  Fragments arranged by size: 

                3747    1881     162      90
 
BstYI r'GATC_y

Cuts at:      0    1346    1370    1767    2165    2744    3314    3335    3557 
   Size:       1346      24     397     398     579     570      21     222

Cuts at:   3557    4988    5880 
   Size:       1431     892

  Fragments arranged by size: 

                1431    1346     892     579     570     398     397     222
                  24      21
 
BtgI C'CryG_G

Cuts at:      0     965    3590    4463    5072    5880 
   Size:        965    2625     873     609     808

  Fragments arranged by size: 

                2625     965     873     808     609
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1770    5175    5880 
   Size:       1770    3405     705

  Fragments arranged by size: 

                3405    1770     705
 
BtsI GCAGTG_nn'

Cuts at:      0    1792    1891    2482    4829    5880 
   Size:       1792      99     591    2347    1051

  Fragments arranged by size: 

                2347    1792    1051     591      99
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1819    2622    5880 
   Size:       1819     803    3258

  Fragments arranged by size: 

                3258    1819     803
 
EaeI y'GGCC_r

Cuts at:      0    1250    1871    1961    2268    2381    2543    2969    3233 
   Size:       1250     621      90     307     113     162     426     264

Cuts at:   3233    3266    3686    3812    4097    4214    4826    4883    5717 
   Size:         33     420     126     285     117     612      57     834

Cuts at:   5717    5880 
   Size:        163

  Fragments arranged by size: 

                1250     834     621     612     426     420     307     285
                 264     163     162     126     117     113      90      57
                  33
 
EagI C'GGCC_G

Cuts at:      0    5717    5880 
   Size:       5717     163
 
EarI CTCTTCn'nnn_

Cuts at:      0     149     389     418     992    1441    2494    2735    3023 
   Size:        149     240      29     574     449    1053     241     288

Cuts at:   3023    3092    3272    3410    4153    4619    5020    5642    5828 
   Size:         69     180     138     743     466     401     622     186

Cuts at:   5828    5880 
   Size:         52

  Fragments arranged by size: 

                1053     743     622     574     466     449     401     288
                 241     240     186     180     149     138      69      52
                  29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     784    2821    3619    4366    4420    4492    5880 
   Size:        784    2037     798     747      54      72    1388

  Fragments arranged by size: 

                2037    1388     798     784     747      72      54
 
EcoICRI GAG'CTC

Cuts at:      0     264    2244    2703    3198    3219    3252    3480    4532 
   Size:        264    1980     459     495      21      33     228    1052

Cuts at:   4532    5880 
   Size:       1348

  Fragments arranged by size: 

                1980    1348    1052     495     459     264     228      33
                  21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     548     640     673     872     883    1024    1480 
   Size:        413     135      92      33     199      11     141     456

Cuts at:   1480    1705    2260    2332    2350    2419    2479    2650    2875 
   Size:        225     555      72      18      69      60     171     225

Cuts at:   2875    2911    2947    2968    3157    3178    3184    3238    3331 
   Size:         36      36      21     189      21       6      54      93

Cuts at:   3331    3379    3535    3547    3634    3685    3937    4054    4084 
   Size:         48     156      12      87      51     252     117      30

Cuts at:   4084    4153    4201    4252    4336    4474    4504    4588    4651 
   Size:         69      48      51      84     138      30      84      63

Cuts at:   4651    4813    4864    4876    4885    5044    5200    5221    5233 
   Size:        162      51      12       9     159     156      21      12

Cuts at:   5233    5275    5323    5476    5551    5635    5656    5719    5880 
   Size:         42      48     153      75      84      21      63     161

  Fragments arranged by size: 

                 555     456     413     252     225     225     199     189
                 171     162     161     159     156     156     153     141
                 138     135     117      93      92      87      84      84
                  84      75      72      69      69      63      63      60
                  54      51      51      51      48      48      48      42
                  36      36      33      30      30      21      21      21
                  21      18      12      12      12      11       9       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     139    1462    1864    2314    2367    3529    3646    4351 
   Size:        139    1323     402     450      53    1162     117     705

Cuts at:   4351    4996    5880 
   Size:        645     884

  Fragments arranged by size: 

                1323    1162     884     705     645     450     402     139
                 117      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1558    1901    2430    3405    4176    4185    4449    4521 
   Size:       1558     343     529     975     771       9     264      72

Cuts at:   4521    4584    4713    4860    5772    5880 
   Size:         63     129     147     912     108

  Fragments arranged by size: 

                1558     975     912     771     529     343     264     147
                 129     108      72      63       9
 
EcoRI G'AATT_C

Cuts at:      0    4014    5880 
   Size:       4014    1866
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     135    4035    5880 
   Size:        135    3900    1845

  Fragments arranged by size: 

                3900    1845     135
 
FspI TGC'GCA

Cuts at:      0    1294    3802    5880 
   Size:       1294    2508    2078

  Fragments arranged by size: 

                2508    2078    1294
 
FspAI rTGC'GCAy

Cuts at:      0    3802    5880 
   Size:       3802    2078
 
HaeII r_GCGC'y

Cuts at:      0    1334    2790    2873    3105    3430    4299    4815    5091 
   Size:       1334    1456      83     232     325     869     516     276

Cuts at:   5091    5490    5816    5880 
   Size:        399     326      64

  Fragments arranged by size: 

                1456    1334     869     516     399     326     325     276
                 232      83      64
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     426     458     679     711    1072    1104    1362    1369 
   Size:        426      32     221      32     361      32     258       7

Cuts at:   1369    1394    1401    2150    2157    2182    2189    3025    3057 
   Size:         25       7     749       7      25       7     836      32

Cuts at:   3057    3313    3345    3346    3378    3469    3501    3868    3900 
   Size:        256      32       1      32      91      32     367      32

Cuts at:   3900    4416    4448    5587    5619    5880 
   Size:        516      32    1139      32     261

  Fragments arranged by size: 

                1139     836     749     516     426     367     361     261
                 258     256     221      91      32      32      32      32
                  32      32      32      32      32      32      25      25
                   7       7       7       7       1
 
HincII GTy'rAC

Cuts at:      0    4680    5880 
   Size:       4680    1200
 
KasI G'GCGC_C

Cuts at:      0    1330    5812    5880 
   Size:       1330    4482      68

  Fragments arranged by size: 

                4482    1330      68
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     729     860    1862    3422    3899    4652    5494 
   Size:        131     598     131    1002    1560     477     753     842

Cuts at:   5494    5852    5880 
   Size:        358      28

  Fragments arranged by size: 

                1560    1002     842     753     598     477     358     131
                 131      28
 
MscI TGG'CCA

Cuts at:      0    1252    1963    2270    2383    2545    2971    3235    3268 
   Size:       1252     711     307     113     162     426     264      33

Cuts at:   3268    3688    5880 
   Size:        420    2192

  Fragments arranged by size: 

                2192    1252     711     426     420     307     264     162
                 113      33
 
MslI CAynn'nnrTG

Cuts at:      0     449    1183    2608    4513    5880 
   Size:        449     734    1425    1905    1367

  Fragments arranged by size: 

                1905    1425    1367     734     449
 
MspA1I CmG'CkG

Cuts at:      0      60     630    1108    1280    1398    1683    1959    2009 
   Size:         60     570     478     172     118     285     276      50

Cuts at:   2009    2073    2439    2508    2583    2652    2853    2889    3231 
   Size:         64     366      69      75      69     201      36     342

Cuts at:   3231    3466    3612    3759    3927    4032    4197    4620    4758 
   Size:        235     146     147     168     105     165     423     138

Cuts at:   4758    4917    5074    5141    5199    5346    5454    5466    5634 
   Size:        159     157      67      58     147     108      12     168

Cuts at:   5634    5661    5880 
   Size:         27     219

  Fragments arranged by size: 

                 570     478     423     366     342     285     276     235
                 219     201     172     168     168     165     159     157
                 147     147     146     138     118     108     105      75
                  69      69      67      64      60      58      50      36
                  27      12
 
NaeI GCC'GGC

Cuts at:      0    1362    1722    1875    2040    2524    5880 
   Size:       1362     360     153     165     484    3356

  Fragments arranged by size: 

                3356    1362     484     360     165     153
 
NarI GG'CG_CC

Cuts at:      0    1331    5813    5880 
   Size:       1331    4482      67

  Fragments arranged by size: 

                4482    1331      67
 
NcoI C'CATG_G

Cuts at:      0     965    4463    5880 
   Size:        965    3498    1417

  Fragments arranged by size: 

                3498    1417     965
 
NgoMIV G'CCGG_C

Cuts at:      0    1360    1720    1873    2038    2522    5880 
   Size:       1360     360     153     165     484    3358

  Fragments arranged by size: 

                3358    1360     484     360     165     153
 
NspI r_CATG'y

Cuts at:      0     954    1881    1926    4953    5880 
   Size:        954     927      45    3027     927

  Fragments arranged by size: 

                3027     954     927     927      45
 
PciI A'CATG_T

Cuts at:      0     950    5880 
   Size:        950    4930
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     570    1131    4347    4566    5880 
   Size:        570     561    3216     219    1314

  Fragments arranged by size: 

                3216    1314     570     561     219
 
PfoI T'CCnGG_A

Cuts at:      0     650     956    1349    1457    2141    2834    2945    3317 
   Size:        650     306     393     108     684     693     111     372

Cuts at:   3317    3347    3376    3560    4022    5880 
   Size:         30      29     184     462    1858

  Fragments arranged by size: 

                1858     693     684     650     462     393     372     306
                 184     111     108      30      29
 
PmlI CAC'GTG

Cuts at:      0     269    5880 
   Size:        269    5611
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1258    1290    1582    1614    5880 
   Size:       1258      32     292      32    4266

  Fragments arranged by size: 

                4266    1258     292      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2430    3405    4176    4521    4713    4860    5772    5880 
   Size:       2430     975     771     345     192     147     912     108

  Fragments arranged by size: 

                2430     975     912     771     345     192     147     108
            
 
PspOMI G'GGCC_C

Cuts at:      0    2708    4448    4583    5880 
   Size:       2708    1740     135    1297

  Fragments arranged by size: 

                2708    1740    1297     135
 
PspXI vC'TCGA_Gb

Cuts at:      0    5434    5880 
   Size:       5434     446
 
PstI C_TGCA'G

Cuts at:      0     638    1430    1526    2371    2516    2624    2657    2804 
   Size:        638     792      96     845     145     108      33     147

Cuts at:   2804    2888    3713    3764    3773    3836    3932    3953    4256 
   Size:         84     825      51       9      63      96      21     303

Cuts at:   4256    4646    4688    4721    4931    5273    5384    5549    5690 
   Size:        390      42      33     210     342     111     165     141

Cuts at:   5690    5880 
   Size:        190

  Fragments arranged by size: 

                 845     825     792     638     390     342     303     210
                 190     165     147     145     141     111     108      96
                  96      84      63      51      42      33      33      21
                   9
 
PvuII CAG'CTG

Cuts at:      0     630    1108    1398    1683    1959    2073    2583    2652 
   Size:        630     478     290     285     276     114     510      69

Cuts at:   2652    2853    2889    3231    3466    3612    3759    3927    4032 
   Size:        201      36     342     235     146     147     168     105

Cuts at:   4032    4620    4758    4917    5199    5346    5454    5466    5634 
   Size:        588     138     159     282     147     108      12     168

Cuts at:   5634    5880 
   Size:        246

  Fragments arranged by size: 

                 630     588     510     478     342     290     285     282
                 276     246     235     201     168     168     159     147
                 147     146     138     114     108     105      69      36
                  12
 
RsrII CG'GwC_CG

Cuts at:      0    5516    5880 
   Size:       5516     364
 
SacI G_AGCT'C

Cuts at:      0     266    2246    2705    3200    3221    3254    3482    4534 
   Size:        266    1980     459     495      21      33     228    1052

Cuts at:   4534    5880 
   Size:       1346

  Fragments arranged by size: 

                1980    1346    1052     495     459     266     228      33
                  21
 
SacII CC_GC'GG

Cuts at:      0    5075    5880 
   Size:       5075     805
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1441    2494    3092    3410    4619    5020    5880 
   Size:       1441    1053     598     318    1209     401     860

  Fragments arranged by size: 

                1441    1209    1053     860     598     401     318
 
SbfI CC_TGCA'GG

Cuts at:      0    2804    4688    4721    5880 
   Size:       2804    1884      33    1159

  Fragments arranged by size: 

                2804    1884    1159      33
 
SexAI A'CCwGG_T

Cuts at:      0    1865    5880 
   Size:       1865    4015
 
SfcI C'TryA_G

Cuts at:      0     471     634    1426    1522    2367    2512    2620    2653 
   Size:        471     163     792      96     845     145     108      33

Cuts at:   2653    2800    2884    3709    3760    3769    3832    3928    3949 
   Size:        147      84     825      51       9      63      96      21

Cuts at:   3949    4252    4642    4684    4717    4927    5269    5380    5545 
   Size:        303     390      42      33     210     342     111     165

Cuts at:   5545    5686    5880 
   Size:        141     194

  Fragments arranged by size: 

                 845     825     792     471     390     342     303     210
                 194     165     163     147     145     141     111     108
                  96      96      84      63      51      42      33      33
                  21       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3265    4696    5662    5880 
   Size:       3265    1431     966     218

  Fragments arranged by size: 

                3265    1431     966     218
 
SfoI GGC'GCC

Cuts at:      0    1332    5814    5880 
   Size:       1332    4482      66

  Fragments arranged by size: 

                4482    1332      66
 
SmaI CCC'GGG

Cuts at:      0     933    1565    1899    4447    5880 
   Size:        933     632     334    2548    1433

  Fragments arranged by size: 

                2548    1433     933     632     334
 
SmlI C'TyrA_G

Cuts at:      0     136     301    2092    2842    3061    3220    3307    3643 
   Size:        136     165    1791     750     219     159      87     336

Cuts at:   3643    3991    5368    5434    5623    5880 
   Size:        348    1377      66     189     257

  Fragments arranged by size: 

                1791    1377     750     348     336     257     219     189
                 165     159     136      87      66
 
SphI G_CATG'C

Cuts at:      0    4953    5880 
   Size:       4953     927
 
SrfI GCCC'GGGC

Cuts at:      0    4447    5880 
   Size:       4447    1433
 
StuI AGG'CCT

Cuts at:      0     344     658    1246    2362    5419    5880 
   Size:        344     314     588    1116    3057     461

  Fragments arranged by size: 

                3057    1116     588     461     344     314
 
StyI C'CwwG_G

Cuts at:      0     242     803     965    1247    1253    2189    2495    2546 
   Size:        242     561     162     282       6     936     306      51

Cuts at:   2546    2972    3408    3899    4427    4463    4535    5480    5880 
   Size:        426     436     491     528      36      72     945     400

  Fragments arranged by size: 

                 945     936     561     528     491     436     426     400
                 306     282     242     162      72      51      36       6
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      87     506     883    1162    1669    5533    5880 
   Size:         87     419     377     279     507    3864     347

  Fragments arranged by size: 

                3864     507     419     377     347     279      87
 
TatI w'GTAC_w

Cuts at:      0     410    5411    5579    5880 
   Size:        410    5001     168     301

  Fragments arranged by size: 

                5001     410     301     168
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1576    1608    3782    3814    5880 
   Size:       1576      32    2174      32    2066

  Fragments arranged by size: 

                2174    2066    1576      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3820    5880 
   Size:       3820    2060
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     168     648     981    1245    1455    2196    3834    4059 
   Size:        168     480     333     264     210     741    1638     225

Cuts at:   4059    4542    4668    5880 
   Size:        483     126    1212

  Fragments arranged by size: 

                1638    1212     741     483     480     333     264     225
                 210     168     126
 
XhoI C'TCGA_G

Cuts at:      0    2092    3643    5434    5880 
   Size:       2092    1551    1791     446

  Fragments arranged by size: 

                2092    1791    1551     446
 
XmaI C'CCGG_G

Cuts at:      0     931    1563    1897    4445    5880 
   Size:        931     632     334    2548    1435

  Fragments arranged by size: 

                2548    1435     931     632     334
 
XmnI GAAnn'nnTTC

Cuts at:      0    4018    5880 
   Size:       4018    1862
 
ZraI GAC'GTC

Cuts at:      0    2343    5880 
   Size:       2343    3537
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    FspAI    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PstI    PvuII
    RsrII     SacI    SacII     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AgeI     AleI     AscI     AseI    AsiSI
    AvrII     BaeI    BciVI    BfrBI     BmtI    BsaWI    BspEI   BssHII
    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII    EcoRV
     FseI  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI    PshAI     PsiI
     PsrI     PvuI     SalI    SanDI     ScaI     SgfI    SgrAI    SnaBI
     SpeI     SspI     SwaI     XbaI
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