Restriction map for pF1KSDA1819
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       BbeI        BbrPI       
BcgI        BfrI        BglII       BsaAI       BsaBI       
BsiEI       BsiWI       BspDI       BspHI       BsrBI       
BssHII      BssSI       Bst1107I    BstBI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoT22I     FspI        KasI        KpnI        
Ksp632I     KspI        MamI        MluI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
StuI        SwaI        XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        2928  GACGTC               GACGT^C
AccI         2538  GTCTAC               GT^[AC][GT]AC
AccII        2331  CGCG                 CG^CG
AccIII       1252  TCCGGA               T^CCGGA
AflIII       1538  ACATGT               A^C[GA][CT]GT
Alw44I         94  GTGCAC               G^TGCAC
ApaLI          94  GTGCAC               G^TGCAC
AvaI           87  CCCGAG               C^[CT]CG[GA]G
AvrII        1057  CCTAGG               C^CTAGG
BamHI         906  GGATCC               G^GATCC
BanI         1571  GGCACC               G^G[CT][GA]CC
BbsI         3390  GAAGAC               GAAGAC(2/6)
BlnI         1057  CCTAGG               C^CTAGG
BpuAI        3390  GAAGAC               GAAGAC(2/6)
BsaWI        1252  TCCGGA               [AT]^CCGG[AT]
BseAI        1252  TCCGGA               T^CCGGA
BsgI         2876  CTGCAC               GTGCAG(16/14)
BsoBI          87  CCCGAG               C^[CT]CG[GA]G
BspEI        1252  TCCGGA               T^CCGGA
BspLU11I     1538  ACATGT               A^CATGT
BsrFI        1549  GCCGGC               [GA]^CCGG[CT]
BstEII       2033  GGTCACC              G^GT[ACGT]ACC
BstPI        2033  GGTCACC              G^GT[ACGT]ACC
BstUI        2331  CGCG                 CG^CG
Cfr10I       1549  GCCGGC               [GA]^CCGG[CT]
DraIII       2137  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2033  GGTCACC              G^GT[ACGT]ACC
EcoRI         614  GAATTC               G^AATTC
EcoRV         604  GATATC               GAT^ATC
FseI         1548  GGCCGGCC             GGCCGG^CC
HindIII      2463  AAGCTT               A^AGCTT
MroI         1252  TCCGGA               T^CCGGA
MvnI         2331  CGCG                 CG^CG
NaeI         1549  GCCGGC               GCC^GGC
NgoMIV       1549  GCCGGC               G^CCGGC
SexAI        1413  ACCAGGT              A^CC[AT]GGT
SmlI         2894  CTCAAG               C^T[CT][GA]AG
SspI          859  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI          614  GAATTC               [GA]^AATT[CT]
             2805  AAATTT              
AcyI         2928  GACGTC               G[GA]^CG[CT]C
             3237  GACGCC              
ApaI         1122  GGGCCC               GGGCC^C
             3345  GGGCCC              
ApoI          614  GAATTC               [GA]^AATT[CT]
             2805  AAATTT              
AspI          671  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              813  GACTCTGTC           
BciVI        2101  GGATAC               GTATCC(6/5)
             1288  GTATCC              
BlpI         1234  GCTCAGC              GC^T[ACGT]AGC
             3150  GCTAAGC             
Bpu1102I     1234  GCTCAGC              GC^T[ACGT]AGC
             3150  GCTAAGC             
BsaHI        2928  GACGTC               G[GA]^CG[CT]C
             3237  GACGCC              
BsaI         2799  GAGACC               GGTCTC(1/5)
             3069  GAGACC              
BsmBI        1202  CGTCTC               CGTCTC(1/5)
              881  GAGACG              
BsmI         2558  GAATGC               GAATGC(1/-1)
             1168  GCATTC              
Bsp1407I      237  TGTACA               T^GTACA
             1541  TGTACA              
BspMI         410  ACCTGC               ACCTGC(4/8)
             2961  ACCTGC              
BsrDI        2088  CATTGC               GCAATG(2/0)
              761  GCAATG              
BsrGI         237  TGTACA               T^GTACA
             1541  TGTACA              
BstAPI        405  GCAGCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2313  GCAGCAGATGC         
CelII        1234  GCTCAGC              GC^T[ACGT]AGC
             3150  GCTAAGC             
EcoNI        1240  CCTCAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2725  CCTACCTTAGG         
HaeII          12  AGCGCC               [GA]GCGC^[CT]
              113  AGCGCC              
HgaI         2329  GACGC                GACGC(5/10)
             3237  GACGC               
Hin1I        2928  GACGTC               G[GA]^CG[CT]C
             3237  GACGCC              
HincII        544  GTTAAC               GT[CT]^[GA]AC
             3048  GTTAAC              
HindII        544  GTTAAC               GT[CT]^[GA]AC
             3048  GTTAAC              
HpaI          544  GTTAAC               GTT^AAC
             3048  GTTAAC              
MaeII        2852  ACGT                 A^CGT
             2929  ACGT                
PflFI         671  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              813  GACTCTGTC           
PpuMI         691  GGGACCT              [GA]G^G[AT]CC[CT]
             3114  GGGTCCC             
PshAI        1027  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2366  GACATTTGTC          
PvuII        1141  CAGCTG               CAG^CTG
             1157  CAGCTG              
SphI         1335  GCATGC               GCATG^C
             2828  GCATGC              
SspBI         237  TGTACA               T^GTACA
             1541  TGTACA              
Tth111I       671  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              813  GACTCTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        AspHI       AvaII       BalI        BanII       
BbvCI       BbvI        BclI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MfeI        MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
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