Restriction map for pF1KSDA1819
 (Linear) (Six-base) MAPSORT of: KIAA1819.seq  Check: 6601  from: 1  to: 3459



KIAA1819.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     419    2970    3459 
   Size:        419    2551     489

  Fragments arranged by size: 

                2551     489     419
 
AatII G_ACGT'C

Cuts at:      0    2932    3459 
   Size:       2932     527
 
AccI GT'mk_AC

Cuts at:      0    2539    3459 
   Size:       2539     920
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1199    2032    2050    2627    3459 
   Size:       1199     833      18     577     832

  Fragments arranged by size: 

                1199     833     832     577      18
 
AflIII A'CryG_T

Cuts at:      0    1538    3459 
   Size:       1538    1921
 
AleI CACnn'nnGTG

Cuts at:      0    3299    3459 
   Size:       3299     160
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2447    3459 
   Size:       2447    1012
 
AlwNI CAG_nnn'CTG

Cuts at:      0     228     411     924    2322    2988    3400    3459 
   Size:        228     183     513    1398     666     412      59

  Fragments arranged by size: 

                1398     666     513     412     228     183      59
 
ApaI G_GGCC'C

Cuts at:      0    1126    3349    3459 
   Size:       1126    2223     110

  Fragments arranged by size: 

                2223    1126     110
 
ApaLI G'TGCA_C

Cuts at:      0      94    3459 
   Size:         94    3365
 
ApoI r'AATT_y

Cuts at:      0     614    2805    3459 
   Size:        614    2191     654

  Fragments arranged by size: 

                2191     654     614
 
AvaI C'yCGr_G

Cuts at:      0      87    3459 
   Size:         87    3372
 
AvrII C'CTAG_G

Cuts at:      0    1057    3459 
   Size:       1057    2402
 
BamHI G'GATC_C

Cuts at:      0     906    3459 
   Size:        906    2553
 
BanI G'GyrC_C

Cuts at:      0    1571    3459 
   Size:       1571    1888
 
BanII G_rGCy'C

Cuts at:      0      65      79     126     196    1126    1483    1652    1894 
   Size:         65      14      47      70     930     357     169     242

Cuts at:   1894    3349    3459 
   Size:       1455     110

  Fragments arranged by size: 

                1455     930     357     242     169     110      70      65
                  47      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3397    3459 
   Size:       3397      62
 
BbvCI CC'TCA_GC

Cuts at:      0    1139    1144    1557    3322    3459 
   Size:       1139       5     413    1765     137

  Fragments arranged by size: 

                1765    1139     413     137       5
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1299    2095    3459 
   Size:       1299     796    1364

  Fragments arranged by size: 

                1364    1299     796
 
BclI T'GATC_A

Cuts at:      0     986    1088    2858    3459 
   Size:        986     102    1770     601

  Fragments arranged by size: 

                1770     986     601     102
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55    1130    1278    3459 
   Size:         55    1075     148    2181

  Fragments arranged by size: 

                2181    1075     148      55
 
BlpI GC'TnA_GC

Cuts at:      0    1235    3151    3459 
   Size:       1235    1916     308

  Fragments arranged by size: 

                1916    1235     308
 
Bme1580I G_kGCm'C

Cuts at:      0      98    1126    1574    3349    3459 
   Size:         98    1028     448    1775     110

  Fragments arranged by size: 

                1775    1028     448     110      98
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2159    2488    2895    3459 
   Size:       2159     329     407     564

  Fragments arranged by size: 

                2159     564     407     329
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     651    1250    3459 
   Size:        651     599    2209

  Fragments arranged by size: 

                2209     651     599
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     911     997    2293    2369    3459 
   Size:        499     412      86    1296      76    1090

  Fragments arranged by size: 

                1296    1090     499     412      86      76
 
Bpu10I CC'TnA_GC

Cuts at:      0     154     229     911    1139    1144    1557    2029    3322 
   Size:        154      75     682     228       5     413     472    1293

Cuts at:   3322    3459 
   Size:        137

  Fragments arranged by size: 

                1293     682     472     413     228     154     137      75
                   5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2879    3459 
   Size:       2879     580
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2793    3063    3459 
   Size:       2793     270     396

  Fragments arranged by size: 

                2793     396     270
 
BsaHI Gr'CG_yC

Cuts at:      0    2929    3238    3459 
   Size:       2929     309     221

  Fragments arranged by size: 

                2929     309     221
 
BsaWI w'CCGG_w

Cuts at:      0    1252    3459 
   Size:       1252    2207
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     297     327     738     768    1188    1218    2095    2125 
   Size:        297      30     411      30     420      30     877      30

Cuts at:   2125    2203    2233    2291    2321    3459 
   Size:         78      30      58      30    1138

  Fragments arranged by size: 

                1138     877     420     411     297      78      58      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1062    1127    1386    3026    3459 
   Size:       1062      65     259    1640     433

  Fragments arranged by size: 

                1640    1062     433     259      65
 
BseYI C'CCAG_C

Cuts at:      0    1298    1441    1638    2222    3241    3459 
   Size:       1298     143     197     584    1019     218

  Fragments arranged by size: 

                1298    1019     584     218     197     143
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2861    3459 
   Size:       2861     598
 
BsiHKAI G_wGCw'C

Cuts at:      0      98     196    1054    1483    1894    3459 
   Size:         98      98     858     429     411    1565

  Fragments arranged by size: 

                1565     858     429     411      98      98
 
BsmI GAATG_Cn'

Cuts at:      0    1168    2564    3459 
   Size:       1168    1396     895

  Fragments arranged by size: 

                1396    1168     895
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     875    1208    3459 
   Size:        875     333    2251

  Fragments arranged by size: 

                2251     875     333
 
Bsp1286I G_dGCh'C

Cuts at:      0      65      79      98     126     196    1054    1126    1483 
   Size:         65      14      19      28      70     858      72     357

Cuts at:   1483    1574    1652    1894    3349    3459 
   Size:         91      78     242    1455     110

  Fragments arranged by size: 

                1455     858     357     242     110      91      78      72
                  70      65      28      19      14
 
BspEI T'CCGG_A

Cuts at:      0    1252    3459 
   Size:       1252    2207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     419    2970    3459 
   Size:        419    2551     489

  Fragments arranged by size: 

                2551     489     419
 
BsrDI GCAATG_nn'

Cuts at:      0     768    2087    3459 
   Size:        768    1319    1372

  Fragments arranged by size: 

                1372    1319     768
 
BsrFI r'CCGG_y

Cuts at:      0    1549    3459 
   Size:       1549    1910
 
BsrGI T'GTAC_A

Cuts at:      0     237    1541    3459 
   Size:        237    1304    1918

  Fragments arranged by size: 

                1918    1304     237
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     411    2319    3459 
   Size:        411    1908    1140

  Fragments arranged by size: 

                1908    1140     411
 
BstEII G'GTnAC_C

Cuts at:      0    2033    3459 
   Size:       2033    1426
 
BstYI r'GATC_y

Cuts at:      0     906    1605    2773    3459 
   Size:        906     699    1168     686

  Fragments arranged by size: 

                1168     906     699     686
 
Bsu36I CC'TnA_GG

Cuts at:      0    1128    1601    2730    3121    3459 
   Size:       1128     473    1129     391     338

  Fragments arranged by size: 

                1129    1128     473     391     338
 
BtgI C'CryG_G

Cuts at:      0    1073    1175    1643    2716    3110    3459 
   Size:       1073     102     468    1073     394     349

  Fragments arranged by size: 

                1073    1073     468     394     349     102
 
BtsI GCAGTG_nn'

Cuts at:      0     290     444    2986    3035    3065    3306    3459 
   Size:        290     154    2542      49      30     241     153

  Fragments arranged by size: 

                2542     290     241     154     153      49      30
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     414     449    3459 
   Size:        414      35    3010

  Fragments arranged by size: 

                3010     414      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2142    3459 
   Size:       2142    1317
 
EaeI y'GGCC_r

Cuts at:      0     246     840    1178    1512    2719    3459 
   Size:        246     594     338     334    1207     740

  Fragments arranged by size: 

                1207     740     594     338     334     246
 
EcoICRI GAG'CTC

Cuts at:      0     194    1481    1892    3459 
   Size:        194    1287     411    1567

  Fragments arranged by size: 

                1567    1287     411     194
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     911     997    1199    2032    2050    2293    2369 
   Size:        499     412      86     202     833      18     243      76

Cuts at:   2369    2627    3459 
   Size:        258     832

  Fragments arranged by size: 

                 833     832     499     412     258     243     202      86
                  76      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1244    2729    3459 
   Size:       1244    1485     730

  Fragments arranged by size: 

                1485    1244     730
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24      47     692    1061    1122    1123    1148    2161 
   Size:         24      23     645     369      61       1      25    1013

Cuts at:   2161    3115    3346    3459 
   Size:        954     231     113

  Fragments arranged by size: 

                1013     954     645     369     231     113      61      25
                  24      23       1
 
EcoRI G'AATT_C

Cuts at:      0     614    3459 
   Size:        614    2845
 
EcoRV GAT'ATC

Cuts at:      0     606    3459 
   Size:        606    2853
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2039    2054    3459 
   Size:       2039      15    1405

  Fragments arranged by size: 

                2039    1405      15
 
FseI GG_CCGG'CC

Cuts at:      0    1553    3459 
   Size:       1553    1906
 
HaeII r_GCGC'y

Cuts at:      0      16     117    3459 
   Size:         16     101    3342

  Fragments arranged by size: 

                3342     101      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     717     749     802     834    2093    2115    2122    2125 
   Size:        717      32      53      32    1259      22       7       3

Cuts at:   2125    2147    2154    3459 
   Size:         22       7    1305

  Fragments arranged by size: 

                1305    1259     717      53      32      32      22      22
                   7       7       3
 
HincII GTy'rAC

Cuts at:      0     546    3050    3459 
   Size:        546    2504     409

  Fragments arranged by size: 

                2504     546     409
 
HindIII A'AGCT_T

Cuts at:      0    2463    3459 
   Size:       2463     996
 
HpaI GTT'AAC

Cuts at:      0     546    3050    3459 
   Size:        546    2504     409

  Fragments arranged by size: 

                2504     546     409
 
MfeI C'AATT_G

Cuts at:      0    2245    2656    2683    2794    3459 
   Size:       2245     411      27     111     665

  Fragments arranged by size: 

                2245     665     411     111      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2891    3459 
   Size:       2891     568
 
MscI TGG'CCA

Cuts at:      0     248     842    1180    3459 
   Size:        248     594     338    2279

  Fragments arranged by size: 

                2279     594     338     248
 
MslI CAynn'nnrTG

Cuts at:      0     836     949    1577    3299    3459 
   Size:        836     113     628    1722     160

  Fragments arranged by size: 

                1722     836     628     160     113
 
MspA1I CmG'CkG

Cuts at:      0    1143    1159    1511    3459 
   Size:       1143      16     352    1948

  Fragments arranged by size: 

                1948    1143     352      16
 
NaeI GCC'GGC

Cuts at:      0    1551    3459 
   Size:       1551    1908
 
NcoI C'CATG_G

Cuts at:      0    1073    1175    1643    3110    3459 
   Size:       1073     102     468    1467     349

  Fragments arranged by size: 

                1467    1073     468     349     102
 
NgoMIV G'CCGG_C

Cuts at:      0    1549    3459 
   Size:       1549    1910
 
NspI r_CATG'y

Cuts at:      0    1339    1542    2832    3459 
   Size:       1339     203    1290     627

  Fragments arranged by size: 

                1339    1290     627     203
 
PasI CC'CwG_GG

Cuts at:      0     481    1563    3459 
   Size:        481    1082    1896

  Fragments arranged by size: 

                1896    1082     481
 
PciI A'CATG_T

Cuts at:      0    1538    3459 
   Size:       1538    1921
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1159    2200    2484    2820    3459 
   Size:       1159    1041     284     336     639

  Fragments arranged by size: 

                1159    1041     639     336     284
 
PpuMI rG'GwC_Cy

Cuts at:      0     692    3115    3459 
   Size:        692    2423     344

  Fragments arranged by size: 

                2423     692     344
 
PshAI GACnn'nnGTC

Cuts at:      0    1031    2370    3459 
   Size:       1031    1339    1089

  Fragments arranged by size: 

                1339    1089    1031
 
PsiI TTA'TAA

Cuts at:      0    2393    3459 
   Size:       2393    1066
 
PspOMI G'GGCC_C

Cuts at:      0    1122    3345    3459 
   Size:       1122    2223     114

  Fragments arranged by size: 

                2223    1122     114
 
PstI C_TGCA'G

Cuts at:      0     642     920    2937    2967    3459 
   Size:        642     278    2017      30     492

  Fragments arranged by size: 

                2017     642     492     278      30
 
PvuII CAG'CTG

Cuts at:      0    1143    1159    3459 
   Size:       1143      16    2300

  Fragments arranged by size: 

                2300    1143      16
 
SacI G_AGCT'C

Cuts at:      0     196    1483    1894    3459 
   Size:        196    1287     411    1565

  Fragments arranged by size: 

                1565    1287     411     196
 
SanDI GG'GwC_CC

Cuts at:      0    3115    3459 
   Size:       3115     344
 
SexAI A'CCwGG_T

Cuts at:      0    1413    3459 
   Size:       1413    2046
 
SfcI C'TryA_G

Cuts at:      0     305     638     916    2269    2573    2933    2963    3459 
   Size:        305     333     278    1353     304     360      30     496

  Fragments arranged by size: 

                1353     496     360     333     305     304     278      30
            
 
SmlI C'TyrA_G

Cuts at:      0    2894    3459 
   Size:       2894     565
 
SphI G_CATG'C

Cuts at:      0    1339    2832    3459 
   Size:       1339    1493     627

  Fragments arranged by size: 

                1493    1339     627
 
SspI AAT'ATT

Cuts at:      0     861    3459 
   Size:        861    2598
 
StyI C'CwwG_G

Cuts at:      0      66     278    1057    1073    1175    1643    3110    3459 
   Size:         66     212     779      16     102     468    1467     349

  Fragments arranged by size: 

                1467     779     468     349     212     102      66      16
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1652    1774    3273    3459 
   Size:       1652     122    1499     186

  Fragments arranged by size: 

                1652    1499     186     122
 
TatI w'GTAC_w

Cuts at:      0     237    1541    3459 
   Size:        237    1304    1918

  Fragments arranged by size: 

                1918    1304     237
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1188    1220    3459 
   Size:       1188      32    2239

  Fragments arranged by size: 

                2239    1188      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     674     816    3459 
   Size:        674     142    2643

  Fragments arranged by size: 

                2643     674     142
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     582     664    2094    3097    3459 
   Size:        582      82    1430    1003     362

  Fragments arranged by size: 

                1430    1003     582     362      82
 
ZraI GAC'GTC

Cuts at:      0    2930    3459 
   Size:       2930     529
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI     BglI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstYI   Bsu36I
     BtgI     BtsI    CspCI   DraIII     EaeI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    HaeII    Hin4I   HincII
  HindIII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI     PasI     PciI    PflMI    PpuMI    PshAI
     PsiI   PspOMI     PstI    PvuII     SacI    SanDI    SexAI     SfcI
     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI     BaeI     BbeI     BcgI    BfrBI    BglII    BmgBI
     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI    BsrBI   BssHII
    BssSI    BstBI    BstXI  BstZ17I    BtgZI     ClaI     DraI     DrdI
     EagI     EarI     EciI     FspI    FspAI     KasI     KpnI     MluI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PfoI
     PmeI     PmlI     PpiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI     XbaI     XhoI
     XmaI     XmnI
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