Restriction map for pF1KSDA1734
##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AflIII      
AgeI        ApoI        AscI        AseI        AsnI        
AviII       BbrPI       BciVI       BfrI        BsaBI       
BseAI       BsiWI       BspDI       BspEI       BspHI       
BspLU11I    BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
MamI        MfeI        MluI        MroI        MunI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        ScaI        SfiI        SfuI        
SnaBI       SpeI        SspI        SwaI        XbaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        377  GGTACC               G^GTACC
AhdI          190  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1052  AGCGCT               AGC^GCT
ApaI         2970  GGGCCC               GGGCC^C
Asp718I       377  GGTACC               G^GTACC
AspEI         190  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         996  CCTAGG               C^CTAGG
BamHI           6  GGATCC               G^GATCC
BclI         2324  TGATCA               T^GATCA
BglII         812  AGATCT               A^GATCT
BlnI          996  CCTAGG               C^CTAGG
BsaAI         361  TACGTG               [CT]AC^GT[GA]
BsaWI        3722  ACCGGA               [AT]^CCGG[AT]
BsiEI        1839  CGGCCG               CG[GA][CT]^CG
BsmI         1693  GAATGC               GAATGC(1/-1)
Bsp1407I     2120  TGTACA               T^GTACA
BsrGI        2120  TGTACA               T^GTACA
EagI         1839  CGGCCG               C^GGCCG
Eam1105I      190  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1839  CGGCCG               C^GGCCG
Eco47III     1052  AGCGCT               AGC^GCT
Eco52I       1839  CGGCCG               C^GGCCG
FbaI         2324  TGATCA               T^GATCA
KpnI          377  GGTACC               GGTAC^C
MslI         2478  CATGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI         3631  GCTAGC               G^CTAGC
PaeR7I       1604  CTCGAG               C^TCGAG
PpuMI         569  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         745  GACTCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          291  GAGCTC               GAGCT^C
SbfI         3267  CCTGCAGG             CCTGCA^GG
SexAI         191  ACCTGGT              A^CC[AT]GGT
SgrAI         104  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         1220  CCCGGG               CCC^GGG
SphI         3851  GCATGC               GCATG^C
Sse8387I     3267  CCTGCAGG             CCTGCA^GG
SspBI        2120  TGTACA               T^GTACA
Tsp509I      2829  AATT                 ^AATT
XhoI         1604  CTCGAG               C^TCGAG
XmaCI        1220  CCCGGG               C^CCGGG
XmaI         1220  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          637  GTCTAC               GT^[AC][GT]AC
             1807  GTCTAC              
Asp700I      1857  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2856  GAAGTTCTTC          
AspI          955  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1969  GACATCGTC           
BcgI         1308  CGATGGCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              491  GCACGGTGATCG        
BlpI          855  GCTCAGC              GC^T[ACGT]AGC
             1033  GCTCAGC             
Bpu1102I      855  GCTCAGC              GC^T[ACGT]AGC
             1033  GCTCAGC             
BsrDI         609  CATTGC               GCAATG(2/0)
             1932  CATTGC              
BstEII       1479  GGTGACC              G^GT[ACGT]ACC
             1644  GGTGACC             
BstPI        1479  GGTGACC              G^GT[ACGT]ACC
             1644  GGTGACC             
CelII         855  GCTCAGC              GC^T[ACGT]AGC
             1033  GCTCAGC             
DraIII       1209  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3686  CACCCAGTG           
EcoNI        3180  CCTTCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3837  CCTGCTAGAGG         
EcoO65I      1479  GGTGACC              G^GT[ACGT]ACC
             1644  GGTGACC             
HincII        505  GTCAAC               GT[CT]^[GA]AC
              559  GTCAAC              
HindII        505  GTCAAC               GT[CT]^[GA]AC
              559  GTCAAC              
KspI          285  CCGCGG               CCGC^GG
              354  CCGCGG              
MseI         1567  TTAA                 T^TAA
             2822  TTAA                
PflFI         955  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1969  GACATCGTC           
PflMI        1227  CCATTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3615  CCAGAGCCTGG         
PstI          574  CTGCAG               CTGCA^G
             3268  CTGCAG              
SacII         285  CCGCGG               CCGC^GG
              354  CCGCGG              
StuI         3264  AGGCCT               AGG^CCT
             3581  AGGCCT              
Tru9I        1567  TTAA                 T^TAA
             2822  TTAA                
Tth111I       955  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1969  GACATCGTC           
Van91I       1227  CCATTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3615  CCAGAGCCTGG         
XmnI         1857  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2856  GAAGTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     XcmI        
XhoII       
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