Restriction map for pF1KSDA1400
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AhdI        AocI        Aor51HI     ApoI        AscI        
Asp718I     AspEI       AspI        AvrII       BamHI       
BbrPI       BbvCI       BciVI       BlnI        BsaI        
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoT22I     FseI        
HindIII     KpnI        MluI        MroI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RsrII       
SalI        SapI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflII        2509  CTTAAG               C^TTAAG
AflIII       1406  ACGTGT               A^C[GA][CT]GT
AgeI         2572  ACCGGT               A^CCGGT
Alw44I        586  GTGCAC               G^TGCAC
ApaI          999  GGGCCC               GGGCC^C
ApaLI         586  GTGCAC               G^TGCAC
AseI          343  ATTAAT               AT^TAAT
AsnI          343  ATTAAT               AT^TAAT
Asp700I      1608  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI          530  GCAACCGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2763  TGATCA               T^GATCA
BfrI         2509  CTTAAG               C^TTAAG
BglI         1717  GCCATCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         117  AGATCT               A^GATCT
BsaBI        2758  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2572  ACCGGT               [AT]^CCGG[AT]
BsiEI        1464  CGACCG               CG[GA][CT]^CG
BsmBI        1196  GAGACG               CGTCTC(1/5)
BspHI        2766  TCATGA               T^CATGA
BssHII       2329  GCGCGC               G^CGCGC
BssSI         802  CTCGTG               CACGAG(-5/-1)
BstAPI        579  GCAAGCGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1789  GGTTACC              G^GT[ACGT]ACC
BstPI        1789  GGTTACC              G^GT[ACGT]ACC
EcoNI        1729  CCTCTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1789  GGTTACC              G^GT[ACGT]ACC
EcoRV        2596  GATATC               GAT^ATC
FbaI         2763  TGATCA               T^GATCA
HpaI         2683  GTTAAC               GTT^AAC
KspI          605  CCGCGG               CCGC^GG
MamI         2758  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2814  CAATTG               C^AATTG
MunI         2814  CAATTG               C^AATTG
NcoI         1986  CCATGG               C^CATGG
NspI         2919  GCATGT               [GA]CATG^[CT]
PaeR7I       1507  CTCGAG               C^TCGAG
PflMI        1653  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        2572  ACCGGT               A^CCGGT
PshAI        2728  GACAGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         343  ATTAAT               AT^TAAT
PstI         2517  CTGCAG               CTGCA^G
RcaI         2766  TCATGA               T^CATGA
SacII         605  CCGCGG               CCGC^GG
ScaI         1041  AGTACT               AGT^ACT
StuI          666  AGGCCT               AGG^CCT
TfiI          536  GATTC                G^A[AT]TC
Van91I       1653  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1507  CTCGAG               C^TCGAG
XmnI         1608  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          760  GTCTAC               GT^[AC][GT]AC
             1399  GTCTAC              
AlwNI        1654  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2945  CAGACACTG           
AviII        1017  TGCGCA               TGC^GCA
             1913  TGCGCA              
BalI         2192  TGGCCA               TGG^CCA
             2255  TGGCCA              
BbsI         1218  GTCTTC               GAAGAC(2/6)
             1534  GTCTTC              
BlpI          541  GCTGAGC              GC^T[ACGT]AGC
             2652  GCTCAGC             
Bpu1102I      541  GCTGAGC              GC^T[ACGT]AGC
             2652  GCTCAGC             
BpuAI        1218  GTCTTC               GAAGAC(2/6)
             1534  GTCTTC              
BsmI          436  GCATTC               GAATGC(1/-1)
             2695  GCATTC              
CelII         541  GCTGAGC              GC^T[ACGT]AGC
             2652  GCTCAGC             
DrdI          751  GACCAACCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2475  GACCCCTGAGTC        
EarI         2806  CTCTTC               CTCTTC(1/4)
              955  GAAGAG              
FspI         1017  TGCGCA               TGC^GCA
             1913  TGCGCA              
Ksp632I      2806  CTCTTC               CTCTTC(1/4)
              955  GAAGAG              
MluNI        2192  TGGCCA               TGG^CCA
             2255  TGGCCA              
MscI         2192  TGGCCA               TGG^CCA
             2255  TGGCCA              
PpuMI         178  AGGACCC              [GA]G^G[AT]CC[CT]
              992  AGGACCT             
SacI         2373  GAGCTC               GAGCT^C
             2650  GAGCTC              
SfcI         1295  CTGTAG               C^T[GA][CT]AG
             2517  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BfaI        
BmyI        BpmI        BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfoI        
SmaI        SmlI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI        
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