Restriction map for pF1KSDA1400
 (Linear) (Six-base) MAPSORT of: KIAA1400.seq  Check: 5570  from: 1  to: 3120



KIAA1400.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1164    3120 
   Size:       1164    1956
 
AccI GT'mk_AC

Cuts at:      0     761    1400    3120 
   Size:        761     639    1720

  Fragments arranged by size: 

                1720     761     639
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     849    1062    1599    1627    3120 
   Size:        134     715     213     537      28    1493

  Fragments arranged by size: 

                1493     715     537     213     134      28
 
AflII C'TTAA_G

Cuts at:      0    2509    3120 
   Size:       2509     611
 
AflIII A'CryG_T

Cuts at:      0    1406    3120 
   Size:       1406    1714
 
AgeI A'CCGG_T

Cuts at:      0    2572    3120 
   Size:       2572     548
 
AleI CACnn'nnGTG

Cuts at:      0      97     802    1126    1804    2020    2953    3120 
   Size:         97     705     324     678     216     933     167

  Fragments arranged by size: 

                 933     705     678     324     216     167      97
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2932    3120 
   Size:       2932     188
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1659    2950    3120 
   Size:       1659    1291     170

  Fragments arranged by size: 

                1659    1291     170
 
ApaI G_GGCC'C

Cuts at:      0    1003    3120 
   Size:       1003    2117
 
ApaLI G'TGCA_C

Cuts at:      0     586    3120 
   Size:        586    2534
 
AseI AT'TA_AT

Cuts at:      0     344    3120 
   Size:        344    2776
 
AvaI C'yCGr_G

Cuts at:      0     884    1304    1507    1634    2324    3120 
   Size:        884     420     203     127     690     796

  Fragments arranged by size: 

                 884     796     690     420     203     127
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1342    1375    3120 
   Size:       1342      33    1745

  Fragments arranged by size: 

                1745    1342      33
 
BanI G'GyrC_C

Cuts at:      0     165     454    1113    1435    1477    1725    2041    2403 
   Size:        165     289     659     322      42     248     316     362

Cuts at:   2403    3120 
   Size:        717

  Fragments arranged by size: 

                 717     659     362     322     316     289     248     165
                  42
 
BanII G_rGCy'C

Cuts at:      0     265     964    1003    2054    2170    2377    2654    3120 
   Size:        265     699      39    1051     116     207     277     466

  Fragments arranged by size: 

                1051     699     466     277     265     207     116      39
            
 
BbeI G_GCGC'C

Cuts at:      0    1117    1439    1481    1729    2045    3120 
   Size:       1117     322      42     248     316    1075

  Fragments arranged by size: 

                1117    1075     322     316     248      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1211    1527    3120 
   Size:       1211     316    1593

  Fragments arranged by size: 

                1593    1211     316
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     519     553    3120 
   Size:        519      34    2567

  Fragments arranged by size: 

                2567     519      34
 
BclI T'GATC_A

Cuts at:      0    2763    3120 
   Size:       2763     357
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1723    3120 
   Size:       1723    1397
 
BglII A'GATC_T

Cuts at:      0     117    3120 
   Size:        117    3003
 
BlpI GC'TnA_GC

Cuts at:      0     542    2653    3120 
   Size:        542    2111     467

  Fragments arranged by size: 

                2111     542     467
 
Bme1580I G_kGCm'C

Cuts at:      0     170     457     590     743    1003    2787    3120 
   Size:        170     287     133     153     260    1784     333

  Fragments arranged by size: 

                1784     333     287     260     170     153     133
 
BmgBI CAC'GTC

Cuts at:      0     450     510    1200    1407    3120 
   Size:        450      60     690     207    1713

  Fragments arranged by size: 

                1713     690     450     207      60
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     958    1201    2099    2381    2781    3120 
   Size:        958     243     898     282     400     339

  Fragments arranged by size: 

                 958     898     400     339     282     243
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2668    3120 
   Size:       2668     452
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     304     316     328     574     949    1736    2951 
   Size:        223      81      12      12     246     375     787    1215

Cuts at:   2951    3058    3120 
   Size:        107      62

  Fragments arranged by size: 

                1215     787     375     246     223     107      81      62
                  12      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159    1198    3120 
   Size:        159    1039    1922

  Fragments arranged by size: 

                1922    1039     159
 
BsaAI yAC'GTr

Cuts at:      0     222     597     981    3120 
   Size:        222     375     384    2139

  Fragments arranged by size: 

                2139     384     375     222
 
BsaBI GATnn'nnATC

Cuts at:      0    2762    3120 
   Size:       2762     358
 
BsaHI Gr'CG_yC

Cuts at:      0    1114    1436    1478    1726    2042    2889    3120 
   Size:       1114     322      42     248     316     847     231

  Fragments arranged by size: 

                1114     847     322     316     248     231      42
 
BsaWI w'CCGG_w

Cuts at:      0    2572    3120 
   Size:       2572     548
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     632     644    1177    2428    3120 
   Size:         91     541      12     533    1251     692

  Fragments arranged by size: 

                1251     692     541     533      91      12
 
BseYI C'CCAG_C

Cuts at:      0      53     677    1487    1949    2975    3120 
   Size:         53     624     810     462    1026     145

  Fragments arranged by size: 

                1026     810     624     462     145      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     532    1198    2389    3120 
   Size:        262     270     666    1191     731

  Fragments arranged by size: 

                1191     731     666     270     262
 
BsiEI CG_ry'CG

Cuts at:      0    1467    3120 
   Size:       1467    1653
 
BsiHKAI G_wGCw'C

Cuts at:      0     590    2096    2377    2549    2654    3120 
   Size:        590    1506     281     172     105     466

  Fragments arranged by size: 

                1506     590     466     281     172     105
 
BsmI GAATG_Cn'

Cuts at:      0     436    2695    3120 
   Size:        436    2259     425

  Fragments arranged by size: 

                2259     436     425
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1190    3120 
   Size:       1190    1930
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     265     457     590     743     964    1003    2054 
   Size:        170      95     192     133     153     221      39    1051

Cuts at:   2054    2096    2170    2377    2549    2654    2787    3120 
   Size:         42      74     207     172     105     133     333

  Fragments arranged by size: 

                1051     333     221     207     192     172     170     153
                 133     133     105      95      74      42      39
 
BspHI T'CATG_A

Cuts at:      0    2766    3120 
   Size:       2766     354
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1164    1802    2314    3120 
   Size:       1164     638     512     806

  Fragments arranged by size: 

                1164     806     638     512
 
BsrBI CCG'CTC

Cuts at:      0    1775    2072    2991    3120 
   Size:       1775     297     919     129

  Fragments arranged by size: 

                1775     919     297     129
 
BsrFI r'CCGG_y

Cuts at:      0     691    1395    2572    3120 
   Size:        691     704    1177     548

  Fragments arranged by size: 

                1177     704     691     548
 
BssHII G'CGCG_C

Cuts at:      0    2329    3120 
   Size:       2329     791
 
BssSI C'ACGA_G

Cuts at:      0     802    3120 
   Size:        802    2318
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     585    3120 
   Size:        585    2535
 
BstEII G'GTnAC_C

Cuts at:      0    1789    3120 
   Size:       1789    1331
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     526     996    2022    2794    3120 
   Size:        526     470    1026     772     326

  Fragments arranged by size: 

                1026     772     526     470     326
 
BstYI r'GATC_y

Cuts at:      0     117    1517    2802    3120 
   Size:        117    1400    1285     318

  Fragments arranged by size: 

                1400    1285     318     117
 
BtgI C'CryG_G

Cuts at:      0     605    1814    1986    2045    3120 
   Size:        605    1209     172      59    1075

  Fragments arranged by size: 

                1209    1075     605     172      59
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2142    3120 
   Size:       2142     978
 
BtsI GCAGTG_nn'

Cuts at:      0    1020    1186    3120 
   Size:       1020     166    1934

  Fragments arranged by size: 

                1934    1020     166
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3080    3115    3120 
   Size:       3080      35       5

  Fragments arranged by size: 

                3080      35       5
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     757    2481    3120 
   Size:        757    1724     639

  Fragments arranged by size: 

                1724     757     639
 
EaeI y'GGCC_r

Cuts at:      0    1808    1934    2192    2207    2255    2313    3120 
   Size:       1808     126     258      15      48      58     807

  Fragments arranged by size: 

                1808     807     258     126      58      48      15
 
EarI CTCTTCn'nnn_

Cuts at:      0     950    2812    3120 
   Size:        950    1862     308

  Fragments arranged by size: 

                1862     950     308
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1198    1795    2093    2309    2475    3120 
   Size:       1198     597     298     216     166     645

  Fragments arranged by size: 

                1198     645     597     298     216     166
 
EcoICRI GAG'CTC

Cuts at:      0    2375    2652    3120 
   Size:       2375     277     468

  Fragments arranged by size: 

                2375     468     277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     134     223     304     316     328     574     849     949 
   Size:        134      89      81      12      12     246     275     100

Cuts at:    949    1062    1599    1627    1736    2951    3058    3120 
   Size:        113     537      28     109    1215     107      62

  Fragments arranged by size: 

                1215     537     275     246     134     113     109     107
                 100      89      81      62      28      12      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1733    3120 
   Size:       1733    1387
 
EcoO109I rG'GnC_Cy

Cuts at:      0     179     993    1000    3021    3120 
   Size:        179     814       7    2021      99

  Fragments arranged by size: 

                2021     814     179      99       7
 
EcoRV GAT'ATC

Cuts at:      0    2598    3120 
   Size:       2598     522
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     200    1239    3120 
   Size:        200    1039    1881

  Fragments arranged by size: 

                1881    1039     200
 
FspI TGC'GCA

Cuts at:      0    1019    1915    3120 
   Size:       1019     896    1205

  Fragments arranged by size: 

                1205    1019     896
 
HaeII r_GCGC'y

Cuts at:      0     404    1117    1439    1460    1481    1660    1729    2045 
   Size:        404     713     322      21      21     179      69     316

Cuts at:   2045    2103    3082    3120 
   Size:         58     979      38

  Fragments arranged by size: 

                 979     713     404     322     316     179      69      58
                  38      21      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1141    1173    2587    2619    2794    2826    3120 
   Size:       1141      32    1414      32     175      32     294

  Fragments arranged by size: 

                1414    1141     294     175      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1680    2667    2685    3120 
   Size:       1680     987      18     435

  Fragments arranged by size: 

                1680     987     435      18
 
HpaI GTT'AAC

Cuts at:      0    2685    3120 
   Size:       2685     435
 
KasI G'GCGC_C

Cuts at:      0    1113    1435    1477    1725    2041    3120 
   Size:       1113     322      42     248     316    1079

  Fragments arranged by size: 

                1113    1079     322     316     248      42
 
MfeI C'AATT_G

Cuts at:      0    2814    3120 
   Size:       2814     306
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     926    1340    2630    2648    3120 
   Size:        926     414    1290      18     472

  Fragments arranged by size: 

                1290     926     472     414      18
 
MscI TGG'CCA

Cuts at:      0    2194    2257    3120 
   Size:       2194      63     863

  Fragments arranged by size: 

                2194     863      63
 
MslI CAynn'nnrTG

Cuts at:      0      97     715     802    1126    1804    2020    2200    2365 
   Size:         97     618      87     324     678     216     180     165

Cuts at:   2365    2953    3120 
   Size:        588     167

  Fragments arranged by size: 

                 678     618     588     324     216     180     167     165
                  97      87
 
MspA1I CmG'CkG

Cuts at:      0     607    1605    1953    2031    2794    3120 
   Size:        607     998     348      78     763     326

  Fragments arranged by size: 

                 998     763     607     348     326      78
 
NarI GG'CG_CC

Cuts at:      0    1114    1436    1478    1726    2042    3120 
   Size:       1114     322      42     248     316    1078

  Fragments arranged by size: 

                1114    1078     322     316     248      42
 
NcoI C'CATG_G

Cuts at:      0    1986    3120 
   Size:       1986    1134
 
NspI r_CATG'y

Cuts at:      0    2923    3120 
   Size:       2923     197
 
PasI CC'CwG_GG

Cuts at:      0     519    2055    3120 
   Size:        519    1536    1065

  Fragments arranged by size: 

                1536    1065     519
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1659    3120 
   Size:       1659    1461
 
PfoI T'CCnGG_A

Cuts at:      0     365    2699    3120 
   Size:        365    2334     421

  Fragments arranged by size: 

                2334     421     365
 
PpuMI rG'GwC_Cy

Cuts at:      0     179     993    3120 
   Size:        179     814    2127

  Fragments arranged by size: 

                2127     814     179
 
PshAI GACnn'nnGTC

Cuts at:      0    2732    3120 
   Size:       2732     388
 
PspOMI G'GGCC_C

Cuts at:      0     999    3120 
   Size:        999    2121
 
PspXI vC'TCGA_Gb

Cuts at:      0    1507    3120 
   Size:       1507    1613
 
PstI C_TGCA'G

Cuts at:      0    2521    3120 
   Size:       2521     599
 
PvuII CAG'CTG

Cuts at:      0    1605    2031    2794    3120 
   Size:       1605     426     763     326

  Fragments arranged by size: 

                1605     763     426     326
 
SacI G_AGCT'C

Cuts at:      0    2377    2654    3120 
   Size:       2377     277     466

  Fragments arranged by size: 

                2377     466     277
 
SacII CC_GC'GG

Cuts at:      0     608    3120 
   Size:        608    2512
 
ScaI AGT'ACT

Cuts at:      0    1043    3120 
   Size:       1043    2077
 
SfcI C'TryA_G

Cuts at:      0    1295    2517    3120 
   Size:       1295    1222     603

  Fragments arranged by size: 

                1295    1222     603
 
SfoI GGC'GCC

Cuts at:      0    1115    1437    1479    1727    2043    3120 
   Size:       1115     322      42     248     316    1077

  Fragments arranged by size: 

                1115    1077     322     316     248      42
 
SmaI CCC'GGG

Cuts at:      0     886    1636    2326    3120 
   Size:        886     750     690     794

  Fragments arranged by size: 

                 886     794     750     690
 
SmlI C'TyrA_G

Cuts at:      0     174    1213    1507    2509    3120 
   Size:        174    1039     294    1002     611

  Fragments arranged by size: 

                1039    1002     611     294     174
 
SrfI GCCC'GGGC

Cuts at:      0    2326    3120 
   Size:       2326     794
 
StuI AGG'CCT

Cuts at:      0     668    3120 
   Size:        668    2452
 
StyI C'CwwG_G

Cuts at:      0     425     989    1986    3120 
   Size:        425     564     997    1134

  Fragments arranged by size: 

                1134     997     564     425
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     584    1280    2509    3120 
   Size:        584     696    1229     611

  Fragments arranged by size: 

                1229     696     611     584
 
TatI w'GTAC_w

Cuts at:      0     857    1041    3120 
   Size:        857     184    2079

  Fragments arranged by size: 

                2079     857     184
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1913    1945    3120 
   Size:       1913      32    1175

  Fragments arranged by size: 

                1913    1175      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     717    1660    2016    2202    3120 
   Size:        717     943     356     186     918

  Fragments arranged by size: 

                 943     918     717     356     186
 
XhoI C'TCGA_G

Cuts at:      0    1507    3120 
   Size:       1507    1613
 
XmaI C'CCGG_G

Cuts at:      0     884    1634    2324    3120 
   Size:        884     750     690     796

  Fragments arranged by size: 

                 884     796     750     690
 
XmnI GAAnn'nnTTC

Cuts at:      0    1612    3120 
   Size:       1612    1508
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     AseI     AvaI     BaeI     BanI    BanII
     BbeI     BbsI     BcgI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII     HpaI     KasI     MfeI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PasI
    PflMI     PfoI    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII
     SacI    SacII     ScaI     SfcI     SfoI     SmaI     SmlI     SrfI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AhdI     AloI     ApoI     AscI
    AsiSI    AvrII    BamHI    BbvCI    BciVI    BfrBI     BmtI   Bpu10I
     BsaI    BsaXI    BsiWI    BspEI    BsrDI    BsrGI    BstBI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     EagI    EcoRI     FseI    FspAI
  HindIII     KpnI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI     PsiI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SspI     SwaI
  Tth111I     XbaI     ZraI
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