Restriction map for pF1KSDA1027
##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

Acc65I      AclI        AgeI        AscI        AseI        
AsnI        Asp718I     AvrII       BbrPI       BciVI       
BclI        BlnI        BsaBI       BsiEI       BsiWI       
BssHII      Bst1107I    BstBI       BstZ17I     DraI        
EagI        EclXI       Eco52I      EcoRI       EcoT22I     
FbaI        HpaI        KpnI        KspI        MamI        
MfeI        MluI        MunI        NdeI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        SacII       SalI        SbfI        SfuI        
SgrAI       SnaBI       Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        3423  GACGTC               GACGT^C
AccIII       3101  TCCGGA               T^CCGGA
AcsI         1383  GAATTT               [GA]^AATT[CT]
AflIII       4157  ACATGT               A^C[GA][CT]GT
AhdI         1264  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         1383  GAATTT               [GA]^AATT[CT]
AspEI        1264  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2503  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         104  GGATCC               G^GATCC
BbeI         5803  GGCGCC               GGCGC^C
BsaAI         892  TACGTG               [CT]AC^GT[GA]
BsaWI        3101  TCCGGA               [AT]^CCGG[AT]
BseAI        3101  TCCGGA               T^CCGGA
BspDI        1186  ATCGAT               AT^CGAT
BspEI        3101  TCCGGA               T^CCGGA
BspLU11I     4157  ACATGT               A^CATGT
ClaI         1186  ATCGAT               AT^CGAT
CpoI         4115  CGGTCCG              CG^G[AT]CCG
Eam1105I     1264  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV        1189  GATATC               GAT^ATC
FseI         4899  GGCCGGCC             GGCCGG^CC
KasI         5803  GGCGCC               G^GCGCC
MroI         3101  TCCGGA               T^CCGGA
NarI         5803  GGCGCC               GG^CGCC
NheI          900  GCTAGC               G^CTAGC
PflFI        2503  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         523  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        4115  CGGTCCG              CG^G[AT]CCG
SapI         7441  GAAGAGC              GCTCTTC(1/4)
ScaI         5207  AGTACT               AGT^ACT
SexAI        2504  ACCTGGT              A^CC[AT]GGT
SfiI         5688  GGCCTCGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         5803  GGCGCC               GGC^GCC
SspI          377  AATATT               AAT^ATT
Tth111I      2503  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3177  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Alw44I       4056  GTGCAC               G^TGCAC
             7070  GTGCAC              
Aor51HI      2941  AGCGCT               AGC^GCT
             5947  AGCGCT              
ApaLI        4056  GTGCAC               G^TGCAC
             7070  GTGCAC              
Asp700I       786  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             7519  GAAATGCTTC          
BcgI         4311  CGAGGCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3771  GCAGGCCTCTCG        
BsmI         2718  GAATGC               GAATGC(1/-1)
             4748  GCATTC              
BspHI         335  TCATGA               T^CATGA
              369  TCATGA              
BssSI         562  CACGAG               CACGAG(-5/-1)
              656  CACGAG              
Eco47III     2941  AGCGCT               AGC^GCT
             5947  AGCGCT              
HincII       1284  GTCAAC               GT[CT]^[GA]AC
             6804  GTTGAC              
HindII       1284  GTCAAC               GT[CT]^[GA]AC
             6804  GTTGAC              
PaeR7I       3212  CTCGAG               C^TCGAG
             3803  CTCGAG              
RcaI          335  TCATGA               T^CATGA
              369  TCATGA              
SpeI         2145  ACTAGT               A^CTAGT
             7107  ACTAGT              
XhoI         3212  CTCGAG               C^TCGAG
             3803  CTCGAG              
XmnI          786  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             7519  GAAATGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  152 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflII       AluI        AlwI        AlwNI       AocI        
ApaI        AspHI       AvaI        AvaII       AviII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BfrI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SphI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com