Restriction map for pF1KSDA1027
 (Linear) (Six-base) MAPSORT of: KIAA1027.seq  Check: 2543  from: 1  to: 7623



KIAA1027.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3364    6504    7623 
   Size:       3364    3140    1119

  Fragments arranged by size: 

                3364    3140    1119
 
AatII G_ACGT'C

Cuts at:      0    3427    7623 
   Size:       3427    4196
 
AccI GT'mk_AC

Cuts at:      0      64    1249    5013    6887    7623 
   Size:         64    1185    3764    1874     736

  Fragments arranged by size: 

                3764    1874    1185     736      64
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     283     805    1169    1219    1451    2077    3029 
   Size:         37     246     522     364      50     232     626     952

Cuts at:   3029    3816    5186    6079    6814    6881    6997    7351    7586 
   Size:        787    1370     893     735      67     116     354     235

Cuts at:   7586    7623 
   Size:         37

  Fragments arranged by size: 

                1370     952     893     787     735     626     522     364
                 354     246     235     232     116      67      50      37
                  37
 
AfeI AGC'GCT

Cuts at:      0    2943    5949    7623 
   Size:       2943    3006    1674

  Fragments arranged by size: 

                3006    2943    1674
 
AflII C'TTAA_G

Cuts at:      0     432    2568    6385    7623 
   Size:        432    2136    3817    1238

  Fragments arranged by size: 

                3817    2136    1238     432
 
AflIII A'CryG_T

Cuts at:      0    4157    7623 
   Size:       4157    3466
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1269    7623 
   Size:       1269    6354
 
AleI CACnn'nnGTG

Cuts at:      0    4489    7623 
   Size:       4489    3134
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3569    7310    7623 
   Size:       3569    3741     313

  Fragments arranged by size: 

                3741    3569     313
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1908    2374    2667    2748    2850    3270    3315    3433 
   Size:       1908     466     293      81     102     420      45     118

Cuts at:   3433    3585    3798    4027    5311    5766    6468    7623 
   Size:        152     213     229    1284     455     702    1155

  Fragments arranged by size: 

                1908    1284    1155     702     466     455     420     293
                 229     213     152     118     102      81      45
 
ApaI G_GGCC'C

Cuts at:      0     746    4857    5650    6435    7623 
   Size:        746    4111     793     785    1188

  Fragments arranged by size: 

                4111    1188     793     785     746
 
ApaLI G'TGCA_C

Cuts at:      0    4056    7070    7623 
   Size:       4056    3014     553

  Fragments arranged by size: 

                4056    3014     553
 
ApoI r'AATT_y

Cuts at:      0    1383    7623 
   Size:       1383    6240
 
AvaI C'yCGr_G

Cuts at:      0     617    2085    3212    3362    3779    3803    4850    4871 
   Size:        617    1468    1127     150     417      24    1047      21

Cuts at:   4871    4881    5159    6428    6983    7623 
   Size:         10     278    1269     555     640

  Fragments arranged by size: 

                1468    1269    1127    1047     640     617     555     417
                 278     150      24      21      10
 
BamHI G'GATC_C

Cuts at:      0     104    7623 
   Size:        104    7519
 
BanI G'GyrC_C

Cuts at:      0    2169    3082    4068    5803    5920    7129    7623 
   Size:       2169     913     986    1735     117    1209     494

  Fragments arranged by size: 

                2169    1735    1209     986     913     494     117
 
BanII G_rGCy'C

Cuts at:      0     146     746    1898    3298    3897    4391    4793    4857 
   Size:        146     600    1152    1400     599     494     402      64

Cuts at:   4857    5650    5849    6435    6708    6848    6926    7623 
   Size:        793     199     586     273     140      78     697

  Fragments arranged by size: 

                1400    1152     793     697     600     599     586     494
                 402     273     199     146     140      78      64
 
BbeI G_GCGC'C

Cuts at:      0    5807    7623 
   Size:       5807    1816
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      49     298    1033    3923    6067    6524    7623 
   Size:         49     249     735    2890    2144     457    1099

  Fragments arranged by size: 

                2890    2144    1099     735     457     249      49
 
BbvCI CC'TCA_GC

Cuts at:      0    2531    2681    2778    6212    7623 
   Size:       2531     150      97    3434    1411

  Fragments arranged by size: 

                3434    2531    1411     150      97
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3760    3794    4300    4334    7623 
   Size:       3760      34     506      34    3289

  Fragments arranged by size: 

                3760    3289     506      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1477    1519    1585    2263    2371    2521    2767    3118 
   Size:       1477      42      66     678     108     150     246     351

Cuts at:   3118    3562    3611    3640    4291    5341    5695    6439    6949 
   Size:        444      49      29     651    1050     354     744     510

Cuts at:   6949    7623 
   Size:        674

  Fragments arranged by size: 

                1477    1050     744     678     674     651     510     444
                 354     351     246     150     108      66      49      42
                  29
 
BglII A'GATC_T

Cuts at:      0     833    3186    5123    7623 
   Size:        833    2353    1937    2500

  Fragments arranged by size: 

                2500    2353    1937     833
 
BlpI GC'TnA_GC

Cuts at:      0    1892    2804    2945    3297    7623 
   Size:       1892     912     141     352    4326

  Fragments arranged by size: 

                4326    1892     912     352     141
 
Bme1580I G_kGCm'C

Cuts at:      0     351     746     980    1878    2130    3085    4060    4668 
   Size:        351     395     234     898     252     955     975     608

Cuts at:   4668    4857    5650    5862    6435    7074    7623 
   Size:        189     793     212     573     639     549

  Fragments arranged by size: 

                 975     955     898     793     639     608     573     549
                 395     351     252     234     212     189
 
BmgBI CAC'GTC

Cuts at:      0    5889    7623 
   Size:       5889    1734
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3325    3704    4132    4662    5816    6138    7623 
   Size:       3325     379     428     530    1154     322    1485

  Fragments arranged by size: 

                3325    1485    1154     530     428     379     322
 
BmtI G_CTAG'C

Cuts at:      0     904    7623 
   Size:        904    6719
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1350    1647    5094    5898    7623 
   Size:       1350     297    3447     804    1725

  Fragments arranged by size: 

                3447    1725    1350     804     297
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205    1279    1385    1485    1813    2569    2770    4150 
   Size:        205    1074     106     100     328     756     201    1380

Cuts at:   4150    4717    4728    4949    4996    5407    5887    6457    7623 
   Size:        567      11     221      47     411     480     570    1166

  Fragments arranged by size: 

                1380    1166    1074     756     570     567     480     411
                 328     221     205     201     106     100      47      11
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2531    2681    2778    3122    5257    6212    7623 
   Size:       2531     150      97     344    2135     955    1411

  Fragments arranged by size: 

                2531    2135    1411     955     344     150      97
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     898    2035    3976    5066    7623 
   Size:        898    1137    1941    1090    2557

  Fragments arranged by size: 

                2557    1941    1137    1090     898
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     249    5886    6217    6269    7623 
   Size:        249    5637     331      52    1354

  Fragments arranged by size: 

                5637    1354     331     249      52
 
BsaAI yAC'GTr

Cuts at:      0     894    7623 
   Size:        894    6729
 
BsaHI Gr'CG_yC

Cuts at:      0      80    2906    3424    5804    7623 
   Size:         80    2826     518    2380    1819

  Fragments arranged by size: 

                2826    2380    1819     518      80
 
BsaWI w'CCGG_w

Cuts at:      0    3101    7623 
   Size:       3101    4522
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     673     703     913     943    7623 
   Size:        673      30     210      30    6680

  Fragments arranged by size: 

                6680     673     210      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     571    1258    1448    1731    2665    3514    4669    5404 
   Size:        571     687     190     283     934     849    1155     735

Cuts at:   5404    5653    7039    7623 
   Size:        249    1386     584

  Fragments arranged by size: 

                1386    1155     934     849     735     687     584     571
                 283     249     190
 
BseYI C'CCAG_C

Cuts at:      0     117     130     190    1073    1412    1472    1921    2069 
   Size:        117      13      60     883     339      60     449     148

Cuts at:   2069    2374    2455    2843    2924    2938    3308    3739    4061 
   Size:        305      81     388      81      14     370     431     322

Cuts at:   4061    4206    5174    5178    5461    5643    5766    5908    6112 
   Size:        145     968       4     283     182     123     142     204

Cuts at:   6112    6189    7307    7623 
   Size:         77    1118     316

  Fragments arranged by size: 

                1118     968     883     449     431     388     370     339
                 322     316     305     283     204     182     148     145
                 142     123     117      81      81      77      60      60
                  14      13       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670    1537    1745    2761    2878    2920    3140    3401 
   Size:        670     867     208    1016     117      42     220     261

Cuts at:   3401    3460    3790    4304    4493    5419    5835    6215    6694 
   Size:         59     330     514     189     926     416     380     479

Cuts at:   6694    7623 
   Size:        929

  Fragments arranged by size: 

                1016     929     926     867     670     514     479     416
                 380     330     261     220     208     189     117      59
                  42
 
BsiHKAI G_wGCw'C

Cuts at:      0     563    3298    4060    4475    5849    6848    6926    7074 
   Size:        563    2735     762     415    1374     999      78     148

Cuts at:   7074    7622    7623 
   Size:        548       1

  Fragments arranged by size: 

                2735    1374     999     762     563     548     415     148
                  78       1
 
BsmI GAATG_Cn'

Cuts at:      0    2724    4748    7623 
   Size:       2724    2024    2875

  Fragments arranged by size: 

                2875    2724    2024
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     559    1088    4105    7623 
   Size:        559     529    3017    3518

  Fragments arranged by size: 

                3518    3017     559     529
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     351     563     746     980    1878    1898    2130 
   Size:        146     205     212     183     234     898      20     232

Cuts at:   2130    3085    3298    3897    4060    4391    4475    4668    4793 
   Size:        955     213     599     163     331      84     193     125

Cuts at:   4793    4857    5650    5849    5862    6435    6708    6848    6926 
   Size:         64     793     199      13     573     273     140      78

Cuts at:   6926    7074    7622    7623 
   Size:        148     548       1

  Fragments arranged by size: 

                 955     898     793     599     573     548     331     273
                 234     232     213     212     205     199     193     183
                 163     148     146     140     125      84      78      64
                  20      13       1
 
BspEI T'CCGG_A

Cuts at:      0    3101    7623 
   Size:       3101    4522
 
BspHI T'CATG_A

Cuts at:      0     335     369    7623 
   Size:        335      34    7254

  Fragments arranged by size: 

                7254     335      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1916    3340    3364    4262    6504    7278    7623 
   Size:       1916    1424      24     898    2242     774     345

  Fragments arranged by size: 

                2242    1916    1424     898     774     345      24
 
BsrBI CCG'CTC

Cuts at:      0     102    5220    5785    7623 
   Size:        102    5118     565    1838

  Fragments arranged by size: 

                5118    1838     565     102
 
BsrDI GCAATG_nn'

Cuts at:      0     448    1172    2957    3342    4991    5588    5963    6379 
   Size:        448     724    1785     385    1649     597     375     416

Cuts at:   6379    6602    6908    7052    7361    7490    7623 
   Size:        223     306     144     309     129     133

  Fragments arranged by size: 

                1785    1649     724     597     448     416     385     375
                 309     306     223     144     133     129
 
BsrFI r'CCGG_y

Cuts at:      0    1177    2836    3719    4900    7623 
   Size:       1177    1659     883    1181    2723

  Fragments arranged by size: 

                2723    1659    1181    1177     883
 
BsrGI T'GTAC_A

Cuts at:      0     627    4575    7242    7623 
   Size:        627    3948    2667     381

  Fragments arranged by size: 

                3948    2667     627     381
 
BssSI C'ACGA_G

Cuts at:      0     562     656    7623 
   Size:        562      94    6967

  Fragments arranged by size: 

                6967     562      94
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     492    1515    2032    2289    2667    3355    4327    5239 
   Size:        492    1023     517     257     378     688     972     912

Cuts at:   5239    5311    5455    7234    7623 
   Size:         72     144    1779     389

  Fragments arranged by size: 

                1779    1023     972     912     688     517     492     389
                 378     257     144      72
 
BstEII G'GTnAC_C

Cuts at:      0    5350    5613    5790    6363    7623 
   Size:       5350     263     177     573    1260

  Fragments arranged by size: 

                5350    1260     573     263     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     987    1942    2022    2064    2238    3078    3765    4491 
   Size:        987     955      80      42     174     840     687     726

Cuts at:   4491    4593    6096    6270    6360    7074    7149    7623 
   Size:        102    1503     174      90     714      75     474

  Fragments arranged by size: 

                1503     987     955     840     726     714     687     474
                 174     174     102      90      80      75      42
 
BstYI r'GATC_y

Cuts at:      0     104     266     731     833    1625    2582    3186    3429 
   Size:        104     162     465     102     792     957     604     243

Cuts at:   3429    5123    7571    7623 
   Size:       1694    2448      52

  Fragments arranged by size: 

                2448    1694     957     792     604     465     243     162
                 104     102      52
 
Bsu36I CC'TnA_GG

Cuts at:      0    3637    3791    3810    4769    7623 
   Size:       3637     154      19     959    2854

  Fragments arranged by size: 

                3637    2854     959     154      19
 
BtgI C'CryG_G

Cuts at:      0      68     525    1764    2237    2444    3968    4784    5772 
   Size:         68     457    1239     473     207    1524     816     988

Cuts at:   5772    6152    6401    6548    7623 
   Size:        380     249     147    1075

  Fragments arranged by size: 

                1524    1239    1075     988     816     473     457     380
                 249     207     147      68
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2778    3288    3635    7488    7623 
   Size:       2778     510     347    3853     135

  Fragments arranged by size: 

                3853    2778     510     347     135
 
BtsI GCAGTG_nn'

Cuts at:      0    1721    1815    1859    1878    2879    2890    3443    3578 
   Size:       1721      94      44      19    1001      11     553     135

Cuts at:   3578    5108    5240    5330    5600    5818    5996    6254    7168 
   Size:       1530     132      90     270     218     178     258     914

Cuts at:   7168    7391    7623 
   Size:        223     232

  Fragments arranged by size: 

                1721    1530    1001     914     553     270     258     232
                 223     218     178     135     132      94      90      44
                  19      11
 
ClaI AT'CG_AT

Cuts at:      0    1187    7623 
   Size:       1187    6436
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     837     872    4380    4415    6806    6841    6891    6926 
   Size:        837      35    3508      35    2391      35      50      35

Cuts at:   6926    7623 
   Size:        697

  Fragments arranged by size: 

                3508    2391     837     697      50      35      35      35
                  35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2125    3163    4055    4300    5190    7623 
   Size:       2125    1038     892     245     890    2433

  Fragments arranged by size: 

                2433    2125    1038     892     890     245
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1758    6091    6904    7623 
   Size:       1758    4333     813     719

  Fragments arranged by size: 

                4333    1758     813     719
 
EaeI y'GGCC_r

Cuts at:      0    1431    1566    2385    2696    3461    3519    3612    4074 
   Size:       1431     135     819     311     765      58      93     462

Cuts at:   4074    4595    4898    4902    4988    5147    5679    6717    7205 
   Size:        521     303       4      86     159     532    1038     488

Cuts at:   7205    7623 
   Size:        418

  Fragments arranged by size: 

                1431    1038     819     765     532     521     488     462
                 418     311     303     159     135      93      86      58
                   4
 
EarI CTCTTCn'nnn_

Cuts at:      0     398    5723    7436    7541    7623 
   Size:        398    5325    1713     105      82

  Fragments arranged by size: 

                5325    1713     398     105      82
 
EcoICRI GAG'CTC

Cuts at:      0    3296    5847    6846    6924    7623 
   Size:       3296    2551     999      78     699

  Fragments arranged by size: 

                3296    2551     999     699      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     205     283     805    1169    1219    1279    1385 
   Size:         37     168      78     522     364      50      60     106

Cuts at:   1385    1451    1485    1813    2077    2569    2770    3029    3816 
   Size:         66      34     328     264     492     201     259     787

Cuts at:   3816    4150    4717    4728    4949    4996    5186    5407    5887 
   Size:        334     567      11     221      47     190     221     480

Cuts at:   5887    6079    6457    6814    6881    6997    7351    7586    7623 
   Size:        192     378     357      67     116     354     235      37

  Fragments arranged by size: 

                 787     567     522     492     480     378     364     357
                 354     334     328     264     259     235     221     221
                 201     192     190     168     116     106      78      67
                  66      60      50      47      37      37      34      11
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     799     943    1825    3496    5026    5389    5443    7623 
   Size:        799     144     882    1671    1530     363      54    2180

  Fragments arranged by size: 

                2180    1671    1530     882     799     363     144      54
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     428     621     743    1689    1839    2328    3267 
   Size:        114     314     193     122     946     150     489     939

Cuts at:   3267    3531    3795    3960    4643    4854    4875    5019    5424 
   Size:        264     264     165     683     211      21     144     405

Cuts at:   5424    5647    6093    6216    6432    6897    7173    7623 
   Size:        223     446     123     216     465     276     450

  Fragments arranged by size: 

                 946     939     683     489     465     450     446     405
                 314     276     264     264     223     216     211     193
                 165     150     144     123     122     114      21
 
EcoRV GAT'ATC

Cuts at:      0    1191    7623 
   Size:       1191    6432
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     789    1239    7540    7623 
   Size:        789     450    6301      83

  Fragments arranged by size: 

                6301     789     450      83
 
FseI GG_CCGG'CC

Cuts at:      0    4904    7623 
   Size:       4904    2719
 
FspI TGC'GCA

Cuts at:      0    1870    2692    2714    7623 
   Size:       1870     822      22    4909

  Fragments arranged by size: 

                4909    1870     822      22
 
HaeII r_GCGC'y

Cuts at:      0    2748    2945    4569    4636    5807    5951    7623 
   Size:       2748     197    1624      67    1171     144    1672

  Fragments arranged by size: 

                2748    1672    1624    1171     197     144      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     650     682    1231    1255    1263    1287    1564    1596 
   Size:        650      32     549      24       8      24     277      32

Cuts at:   1596    1617    1649    3414    3446    3567    3599    5115    5147 
   Size:         21      32    1765      32     121      32    1516      32

Cuts at:   5147    6266    6298    7330    7362    7623 
   Size:       1119      32    1032      32     261

  Fragments arranged by size: 

                1765    1516    1119    1032     650     549     277     261
                 121      32      32      32      32      32      32      32
                  32      24      24      21       8
 
HincII GTy'rAC

Cuts at:      0    1286    6806    7623 
   Size:       1286    5520     817

  Fragments arranged by size: 

                5520    1286     817
 
HindIII A'AGCT_T

Cuts at:      0     197    1092    3223    7623 
   Size:        197     895    2131    4400

  Fragments arranged by size: 

                4400    2131     895     197
 
KasI G'GCGC_C

Cuts at:      0    5803    7623 
   Size:       5803    1820
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     511    1769    3627    3932    4754    6294    7623 
   Size:        511    1258    1858     305     822    1540    1329

  Fragments arranged by size: 

                1858    1540    1329    1258     822     511     305
 
MscI TGG'CCA

Cuts at:      0    1568    2698    3521    3614    4597    5149    7623 
   Size:       1568    1130     823      93     983     552    2474

  Fragments arranged by size: 

                2474    1568    1130     983     823     552      93
 
MslI CAynn'nnrTG

Cuts at:      0     328     334     500    3925    4051    4489    5101    5593 
   Size:        328       6     166    3425     126     438     612     492

Cuts at:   5593    5777    5986    6529    7623 
   Size:        184     209     543    1094

  Fragments arranged by size: 

                3425    1094     612     543     492     438     328     209
                 184     166     126       6
 
MspA1I CmG'CkG

Cuts at:      0     121     633    1430    1654    1921    2038    2073    2317 
   Size:        121     512     797     224     267     117      35     244

Cuts at:   2317    2632    2664    2680    2710    3007    3057    3517    3549 
   Size:        315      32      16      30     297      50     460      32

Cuts at:   3549    3593    3602    4002    4012    4318    4523    4974    5145 
   Size:         44       9     400      10     306     205     451     171

Cuts at:   5145    5178    5664    5710    6112    6304    6975    7240    7623 
   Size:         33     486      46     402     192     671     265     383

  Fragments arranged by size: 

                 797     671     512     486     460     451     402     400
                 383     315     306     297     267     265     244     224
                 205     192     171     121     117      50      46      44
                  35      33      32      32      30      16      10       9
            
 
NaeI GCC'GGC

Cuts at:      0    1179    2838    4902    7623 
   Size:       1179    1659    2064    2721

  Fragments arranged by size: 

                2721    2064    1659    1179
 
NarI GG'CG_CC

Cuts at:      0    5804    7623 
   Size:       5804    1819
 
NcoI C'CATG_G

Cuts at:      0      68     525    2444    4784    5772    6548    7623 
   Size:         68     457    1919    2340     988     776    1075

  Fragments arranged by size: 

                2340    1919    1075     988     776     457      68
 
NgoMIV G'CCGG_C

Cuts at:      0    1177    2836    4900    7623 
   Size:       1177    1659    2064    2723

  Fragments arranged by size: 

                2723    2064    1659    1177
 
NheI G'CTAG_C

Cuts at:      0     900    7623 
   Size:        900    6723
 
NspI r_CATG'y

Cuts at:      0     216     333    1404    1455    1509    2353    2758    3134 
   Size:        216     117    1071      51      54     844     405     376

Cuts at:   3134    4161    7623 
   Size:       1027    3462

  Fragments arranged by size: 

                3462    1071    1027     844     405     376     216     117
                  54      51
 
PasI CC'CwG_GG

Cuts at:      0    3330    6270    7170    7623 
   Size:       3330    2940     900     453

  Fragments arranged by size: 

                3330    2940     900     453
 
PciI A'CATG_T

Cuts at:      0    4157    7623 
   Size:       4157    3466
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     609    2148    3270    3315    4264    4440    5892    7623 
   Size:        609    1539    1122      45     949     176    1452    1731

  Fragments arranged by size: 

                1731    1539    1452    1122     949     609     176      45
            
 
PfoI T'CCnGG_A

Cuts at:      0    1043    1991    3842    4118    4127    5282    7623 
   Size:       1043     948    1851     276       9    1155    2341

  Fragments arranged by size: 

                2341    1851    1155    1043     948     276       9
 
PpuMI rG'GwC_Cy

Cuts at:      0     428     621    1689    3267    3531    3795    4875    5019 
   Size:        428     193    1068    1578     264     264    1080     144

Cuts at:   5019    5424    6093    6216    6897    7623 
   Size:        405     669     123     681     726

  Fragments arranged by size: 

                1578    1080    1068     726     681     669     428     405
                 264     264     193     144     123
 
PshAI GACnn'nnGTC

Cuts at:      0     527    7623 
   Size:        527    7096
 
PspOMI G'GGCC_C

Cuts at:      0     742    4853    5646    6431    7623 
   Size:        742    4111     793     785    1192

  Fragments arranged by size: 

                4111    1192     793     785     742
 
PspXI vC'TCGA_Gb

Cuts at:      0    3803    7623 
   Size:       3803    3820
 
PstI C_TGCA'G

Cuts at:      0    1298    2037    2115    2348    2775    2802    2984    3438 
   Size:       1298     739      78     233     427      27     182     454

Cuts at:   3438    4011    4482    5046    5115    5235    5321    5709    5813 
   Size:        573     471     564      69     120      86     388     104

Cuts at:   5813    6303    6776    6939    6957    7434    7623 
   Size:        490     473     163      18     477     189

  Fragments arranged by size: 

                1298     739     573     564     490     477     473     471
                 454     427     388     233     189     182     163     120
                 104      86      78      69      27      18
 
PvuII CAG'CTG

Cuts at:      0     121     633    1654    1921    2038    2317    2632    2680 
   Size:        121     512    1021     267     117     279     315      48

Cuts at:   2680    2710    3007    3057    3517    3602    4002    4012    4318 
   Size:         30     297      50     460      85     400      10     306

Cuts at:   4318    4523    4974    5178    5664    6112    6304    6975    7240 
   Size:        205     451     204     486     448     192     671     265

Cuts at:   7240    7623 
   Size:        383

  Fragments arranged by size: 

                1021     671     512     486     460     451     448     400
                 383     315     306     297     279     267     265     205
                 204     192     121     117      85      50      48      30
                  10
 
RsrII CG'GwC_CG

Cuts at:      0    4116    7623 
   Size:       4116    3507
 
SacI G_AGCT'C

Cuts at:      0    3298    5849    6848    6926    7623 
   Size:       3298    2551     999      78     697

  Fragments arranged by size: 

                3298    2551     999     697      78
 
SanDI GG'GwC_CC

Cuts at:      0     621    1689    3531    4875    7623 
   Size:        621    1068    1842    1344    2748

  Fragments arranged by size: 

                2748    1842    1344    1068     621
 
SapI GCTCTTCn'nnn_

Cuts at:      0    7436    7623 
   Size:       7436     187
 
ScaI AGT'ACT

Cuts at:      0    5209    7623 
   Size:       5209    2414
 
SexAI A'CCwGG_T

Cuts at:      0    2504    7623 
   Size:       2504    5119
 
SfcI C'TryA_G

Cuts at:      0    1294    2033    2111    2270    2344    2771    2798    2980 
   Size:       1294     739      78     159      74     427      27     182

Cuts at:   2980    3190    3434    4007    4478    5042    5111    5231    5317 
   Size:        210     244     573     471     564      69     120      86

Cuts at:   5317    5705    5809    6299    6772    6935    6953    7430    7623 
   Size:        388     104     490     473     163      18     477     193

  Fragments arranged by size: 

                1294     739     573     564     490     477     473     471
                 427     388     244     210     193     182     163     159
                 120     104      86      78      74      69      27      18
            
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    5695    7623 
   Size:       5695    1928
 
SfoI GGC'GCC

Cuts at:      0    5805    7623 
   Size:       5805    1818
 
SmaI CCC'GGG

Cuts at:      0     619    4852    4873    5161    6985    7623 
   Size:        619    4233      21     288    1824     638

  Fragments arranged by size: 

                4233    1824     638     619     288      21
 
SmlI C'TyrA_G

Cuts at:      0     432     913    2050    2568    3212    3803    3991    5081 
   Size:        432     481    1137     518     644     591     188    1090

Cuts at:   5081    6385    7623 
   Size:       1304    1238

  Fragments arranged by size: 

                1304    1238    1137    1090     644     591     518     481
                 432     188
 
SpeI A'CTAG_T

Cuts at:      0    2145    7107    7623 
   Size:       2145    4962     516

  Fragments arranged by size: 

                4962    2145     516
 
SphI G_CATG'C

Cuts at:      0    1404    1509    2758    7623 
   Size:       1404     105    1249    4865

  Fragments arranged by size: 

                4865    1404    1249     105
 
SrfI GCCC'GGGC

Cuts at:      0    4852    5161    7623 
   Size:       4852     309    2462

  Fragments arranged by size: 

                4852    2462     309
 
SspI AAT'ATT

Cuts at:      0     379    7623 
   Size:        379    7244
 
StuI AGG'CCT

Cuts at:      0     703    1588    2830    3016    3775    5089    5929    7623 
   Size:        703     885    1242     186     759    1314     840    1694

  Fragments arranged by size: 

                1694    1314    1242     885     840     759     703     186
            
 
StyI C'CwwG_G

Cuts at:      0      68     525     800     881     998    1031    2444    2621 
   Size:         68     457     275      81     117      33    1413     177

Cuts at:   2621    2825    3956    4586    4784    5336    5582    5772    5795 
   Size:        204    1131     630     198     552     246     190      23

Cuts at:   5795    6089    6353    6536    6548    6560    6995    7116    7623 
   Size:        294     264     183      12      12     435     121     507

  Fragments arranged by size: 

                1413    1131     630     552     507     457     435     294
                 275     264     246     204     198     190     183     177
                 121     117      81      68      33      23      12      12
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2338    2437    3905    4075    5227    5389    5425    5461 
   Size:       2338      99    1468     170    1152     162      36      36

Cuts at:   5461    5509    5518    5938    6181    6689    7117    7623 
   Size:         48       9     420     243     508     428     506

  Fragments arranged by size: 

                2338    1468    1152     508     506     428     420     243
                 170     162      99      48      36      36       9
 
TatI w'GTAC_w

Cuts at:      0     239     627    1137    2155    3450    4575    5207    6759 
   Size:        239     388     510    1018    1295    1125     632    1552

Cuts at:   6759    7242    7586    7623 
   Size:        483     344      37

  Fragments arranged by size: 

                1552    1295    1125    1018     632     510     483     388
                 344     239      37
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     673     705    5174    5176    5206    5208    7113    7145 
   Size:        673      32    4469       2      30       2    1905      32

Cuts at:   7145    7623 
   Size:        478

  Fragments arranged by size: 

                4469    1905     673     478      32      32      30       2
                   2
 
Tth111I GACn'n_nGTC

Cuts at:      0    2506    7623 
   Size:       2506    5117
 
XbaI T'CTAG_A

Cuts at:      0    3177    7623 
   Size:       3177    4446
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     736    2502    2604    3471    6738    7071    7623 
   Size:        736    1766     102     867    3267     333     552

  Fragments arranged by size: 

                3267    1766     867     736     552     333     102
 
XhoI C'TCGA_G

Cuts at:      0    3212    3803    7623 
   Size:       3212     591    3820

  Fragments arranged by size: 

                3820    3212     591
 
XmaI C'CCGG_G

Cuts at:      0     617    4850    4871    5159    6983    7623 
   Size:        617    4233      21     288    1824     640

  Fragments arranged by size: 

                4233    1824     640     617     288      21
 
XmnI GAAnn'nnTTC

Cuts at:      0     790    7523    7623 
   Size:        790    6733     100

  Fragments arranged by size: 

                6733     790     100
 
ZraI GAC'GTC

Cuts at:      0    3425    7623 
   Size:       3425    4198
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NspI     PasI     PciI    PflMI     PfoI    PpuMI    PshAI
   PspOMI    PspXI     PstI    PvuII    RsrII     SacI    SanDI     SapI
     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     SmlI     SpeI
     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AgeI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI    BciVI     BclI    BfrBI    BsaBI    BsiEI    BsiWI   BssHII
    BstBI  BstZ17I     DraI     EagI     EciI    EcoRI    FspAI     HpaI
     KpnI     MfeI     MluI     NdeI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI     PsiI     PsrI     PvuI    SacII     SalI
     SbfI     SgfI    SgrAI    SnaBI     SwaI
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