Restriction map for pF1KSDA0959
 (Linear) (Six-base) MAPSORT of: KIAA0959.seq  Check: 5002  from: 1  to: 2304



KIAA0959.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1954    2304 
   Size:       1954     350
 
AatII G_ACGT'C

Cuts at:      0    1894    2304 
   Size:       1894     410
 
AccI GT'mk_AC

Cuts at:      0     957    2304 
   Size:        957    1347
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618     985    1285    2071    2304 
   Size:        618     367     300     786     233

  Fragments arranged by size: 

                 786     618     367     300     233
 
AfeI AGC'GCT

Cuts at:      0    1876    2304 
   Size:       1876     428
 
AflIII A'CryG_T

Cuts at:      0    1985    2304 
   Size:       1985     319
 
AhdI GACnn_n'nnGTC

Cuts at:      0     169    2304 
   Size:        169    2135
 
AlwNI CAG_nnn'CTG

Cuts at:      0     510    1455    1504    1696    2304 
   Size:        510     945      49     192     608

  Fragments arranged by size: 

                 945     608     510     192      49
 
ApoI r'AATT_y

Cuts at:      0     679     969    1153    1351    1797    2304 
   Size:        679     290     184     198     446     507

  Fragments arranged by size: 

                 679     507     446     290     198     184
 
BanI G'GyrC_C

Cuts at:      0     214    1261    2304 
   Size:        214    1047    1043

  Fragments arranged by size: 

                1047    1043     214
 
BanII G_rGCy'C

Cuts at:      0      31     485     544    1639    1708    2304 
   Size:         31     454      59    1095      69     596

  Fragments arranged by size: 

                1095     596     454      69      59      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     476    1805    1946    1976    2304 
   Size:        476    1329     141      30     328

  Fragments arranged by size: 

                1329     476     328     141      30
 
BbvCI CC'TCA_GC

Cuts at:      0    1697    2304 
   Size:       1697     607
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1893    1927    2304 
   Size:       1893      34     377

  Fragments arranged by size: 

                1893     377      34
 
BclI T'GATC_A

Cuts at:      0     416    1583    2304 
   Size:        416    1167     721

  Fragments arranged by size: 

                1167     721     416
 
BglII A'GATC_T

Cuts at:      0     699    2304 
   Size:        699    1605
 
Bme1580I G_kGCm'C

Cuts at:      0    1253    1264    2304 
   Size:       1253      11    1040

  Fragments arranged by size: 

                1253    1040      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      51    2304 
   Size:         51    2253
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1722    1779    2304 
   Size:       1722      57     525

  Fragments arranged by size: 

                1722     525      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     679    1201    2295    2304 
   Size:        143     536     522    1094       9

  Fragments arranged by size: 

                1094     536     522     143       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1697    2304 
   Size:       1697     607
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1002    2304 
   Size:        595     407    1302

  Fragments arranged by size: 

                1302     595     407
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     788    2304 
   Size:        788    1516
 
BsaAI yAC'GTr

Cuts at:      0     686    2304 
   Size:        686    1618
 
BsaBI GATnn'nnATC

Cuts at:      0     940    2304 
   Size:        940    1364
 
BsaHI Gr'CG_yC

Cuts at:      0    1508    1891    2304 
   Size:       1508     383     413

  Fragments arranged by size: 

                1508     413     383
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     613     643    1626    1656    1816    1846    2304 
   Size:        613      30     983      30     160      30     458

  Fragments arranged by size: 

                 983     613     458     160      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1477    1480    1714    1752    1824    2095    2304 
   Size:       1477       3     234      38      72     271     209

  Fragments arranged by size: 

                1477     271     234     209      72      38       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     485    2116    2304 
   Size:        485    1631     188

  Fragments arranged by size: 

                1631     485     188
 
BsiHKAI G_wGCw'C

Cuts at:      0      31     564    1639    2304 
   Size:         31     533    1075     665

  Fragments arranged by size: 

                1075     665     533      31
 
BsmI GAATG_Cn'

Cuts at:      0     429    1067    2304 
   Size:        429     638    1237

  Fragments arranged by size: 

                1237     638     429
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     485     544     564    1253    1264    1639    1708 
   Size:         31     454      59      20     689      11     375      69

Cuts at:   1708    2304 
   Size:        596

  Fragments arranged by size: 

                 689     596     454     375      69      59      31      20
                  11
 
BspHI T'CATG_A

Cuts at:      0     948    2304 
   Size:        948    1356
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     248    1954    2088    2304 
   Size:        248    1706     134     216

  Fragments arranged by size: 

                1706     248     216     134
 
BsrGI T'GTAC_A

Cuts at:      0    1988    2304 
   Size:       1988     316
 
BssSI C'ACGA_G

Cuts at:      0     703    2304 
   Size:        703    1601
 
BstYI r'GATC_y

Cuts at:      0     699    2304 
   Size:        699    1605
 
Bsu36I CC'TnA_GG

Cuts at:      0    1803    2304 
   Size:       1803     501
 
BtgI C'CryG_G

Cuts at:      0    1718    1787    2204    2304 
   Size:       1718      69     417     100

  Fragments arranged by size: 

                1718     417     100      69
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     927    2304 
   Size:        927    1377
 
BtsI GCAGTG_nn'

Cuts at:      0     762    1000    1179    1292    1622    2304 
   Size:        762     238     179     113     330     682

  Fragments arranged by size: 

                 762     682     330     238     179     113
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     552     638     644     745    1531    1543    2304 
   Size:         44     508      86       6     101     786      12     761

  Fragments arranged by size: 

                 786     761     508     101      86      44      12       6
            
 
EcoICRI GAG'CTC

Cuts at:      0      29    1637    2304 
   Size:         29    1608     667

  Fragments arranged by size: 

                1608     667      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     618     679     985    1201    1285    2071    2295 
   Size:        143     475      61     306     216      84     786     224

Cuts at:   2295    2304 
   Size:          9

  Fragments arranged by size: 

                 786     475     306     224     216     143      84      61
                   9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     643     981    1459    2304 
   Size:        643     338     478     845

  Fragments arranged by size: 

                 845     643     478     338
 
EcoO109I rG'GnC_Cy

Cuts at:      0     446    2304 
   Size:        446    1858
 
EcoRI G'AATT_C

Cuts at:      0     679    2304 
   Size:        679    1625
 
EcoRV GAT'ATC

Cuts at:      0    1089    2304 
   Size:       1089    1215
 
FspI TGC'GCA

Cuts at:      0    2191    2304 
   Size:       2191     113
 
HaeII r_GCGC'y

Cuts at:      0    1878    2304 
   Size:       1878     426
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     155     187     905     937    1078    1110    1575    1607 
   Size:        155      32     718      32     141      32     465      32

Cuts at:   1607    2304 
   Size:        697

  Fragments arranged by size: 

                 718     697     465     155     141      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    2006    2304 
   Size:       2006     298
 
HindIII A'AGCT_T

Cuts at:      0     226    2304 
   Size:        226    2078
 
MslI CAynn'nnrTG

Cuts at:      0     529    1231    1963    2304 
   Size:        529     702     732     341

  Fragments arranged by size: 

                 732     702     529     341
 
MspA1I CmG'CkG

Cuts at:      0     153     717    1501    1631    2029    2304 
   Size:        153     564     784     130     398     275

  Fragments arranged by size: 

                 784     564     398     275     153     130
 
NcoI C'CATG_G

Cuts at:      0    1718    1787    2204    2304 
   Size:       1718      69     417     100

  Fragments arranged by size: 

                1718     417     100      69
 
NspI r_CATG'y

Cuts at:      0    1989    2304 
   Size:       1989     315
 
PciI A'CATG_T

Cuts at:      0    1985    2304 
   Size:       1985     319
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     768    1788    2304 
   Size:        768    1020     516

  Fragments arranged by size: 

                1020     768     516
 
PfoI T'CCnGG_A

Cuts at:      0     159    2304 
   Size:        159    2145
 
PmlI CAC'GTG

Cuts at:      0     686    2304 
   Size:        686    1618
 
PstI C_TGCA'G

Cuts at:      0    1007    1214    2304 
   Size:       1007     207    1090

  Fragments arranged by size: 

                1090    1007     207
 
PvuII CAG'CTG

Cuts at:      0     153     717    1501    2304 
   Size:        153     564     784     803

  Fragments arranged by size: 

                 803     784     564     153
 
SacI G_AGCT'C

Cuts at:      0      31    1639    2304 
   Size:         31    1608     665

  Fragments arranged by size: 

                1608     665      31
 
SapI GCTCTTCn'nnn_

Cuts at:      0     552    1531    2304 
   Size:        552     979     773

  Fragments arranged by size: 

                 979     773     552
 
SfcI C'TryA_G

Cuts at:      0    1003    1210    2304 
   Size:       1003     207    1094

  Fragments arranged by size: 

                1094    1003     207
 
SmlI C'TyrA_G

Cuts at:      0     574     981    2304 
   Size:        574     407    1323

  Fragments arranged by size: 

                1323     574     407
 
StyI C'CwwG_G

Cuts at:      0     218    1051    1718    1787    2204    2304 
   Size:        218     833     667      69     417     100

  Fragments arranged by size: 

                 833     667     417     218     100      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1073    1219    2304 
   Size:       1073     146    1085

  Fragments arranged by size: 

                1085    1073     146
 
TatI w'GTAC_w

Cuts at:      0    1988    2304 
   Size:       1988     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1626    1658    2304 
   Size:       1626      32     646

  Fragments arranged by size: 

                1626     646      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1657    2304 
   Size:       1657     647
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1716    2304 
   Size:       1716     588
 
ZraI GAC'GTC

Cuts at:      0    1892    2304 
   Size:       1892     412
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI    AlwNI
     ApoI     BanI    BanII     BbsI    BbvCI     BcgI     BclI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrGI    BssSI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FspI    HaeII    Hin4I   HincII  HindIII     MslI   MspA1I     NcoI
     NspI     PciI    PflMI     PfoI     PmlI     PstI    PvuII     SacI
     SapI     SfcI     SmlI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AleI     AlfI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BciVI    BfrBI     BglI     BlpI    BmgBI     BmtI    BsaWI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI     FalI     FseI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI     XhoI
     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com