Restriction map for pF1KSDA0827
 (Linear) (Six-base) MAPSORT of: KIAA0827.seq  Check: 1906  from: 1  to: 4593



KIAA0827.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0    2827    4593 
   Size:       2827    1766
 
Acc65I G'GTAC_C

Cuts at:      0     588     884    3109    4593 
   Size:        588     296    2225    1484

  Fragments arranged by size: 

                2225    1484     588     296
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     531     865    2296    2411    2660    2744    2761 
   Size:        191     340     334    1431     115     249      84      17

Cuts at:   2761    3371    3433    3493    3991    4593 
   Size:        610      62      60     498     602

  Fragments arranged by size: 

                1431     610     602     498     340     334     249     191
                 115      84      62      60      17
 
AflIII A'CryG_T

Cuts at:      0    2621    4593 
   Size:       2621    1972
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3405    4593 
   Size:       3405    1188
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     322    1710    2495    4593 
   Size:        322    1388     785    2098

  Fragments arranged by size: 

                2098    1388     785     322
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1474    1642    2077    2331    2884    4593 
   Size:       1474     168     435     254     553    1709

  Fragments arranged by size: 

                1709    1474     553     435     254     168
 
ApoI r'AATT_y

Cuts at:      0    2507    3174    4220    4302    4491    4593 
   Size:       2507     667    1046      82     189     102

  Fragments arranged by size: 

                2507    1046     667     189     102      82
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     572     605    2168    2201    4072    4105    4593 
   Size:        572      33    1563      33    1871      33     488

  Fragments arranged by size: 

                1871    1563     572     488      33      33      33
 
BanI G'GyrC_C

Cuts at:      0     588     884    3109    4593 
   Size:        588     296    2225    1484

  Fragments arranged by size: 

                2225    1484     588     296
 
BanII G_rGCy'C

Cuts at:      0     799     882    3385    3649    4593 
   Size:        799      83    2503     264     944

  Fragments arranged by size: 

                2503     944     799     264      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3182    4593 
   Size:       3182    1411
 
BbvCI CC'TCA_GC

Cuts at:      0     327    4593 
   Size:        327    4266
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     862     896    4593 
   Size:        862      34    3697

  Fragments arranged by size: 

                3697     862      34
 
BclI T'GATC_A

Cuts at:      0    4401    4593 
   Size:       4401     192
 
BglII A'GATC_T

Cuts at:      0    1594    1849    4593 
   Size:       1594     255    2744

  Fragments arranged by size: 

                2744    1594     255
 
BlpI GC'TnA_GC

Cuts at:      0      25    4593 
   Size:         25    4568
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    4020    4593 
   Size:       4020     573
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     186     339    3749    4593 
   Size:        186     153    3410     844

  Fragments arranged by size: 

                3410     844     186     153
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     803    1610    2429    4115    4593 
   Size:        164     639     807     819    1686     478

  Fragments arranged by size: 

                1686     819     807     639     478     164
 
Bpu10I CC'TnA_GC

Cuts at:      0     327    4593 
   Size:        327    4266
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3041    3248    3380    4085    4593 
   Size:       3041     207     132     705     508

  Fragments arranged by size: 

                3041     705     508     207     132
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2011    4593 
   Size:       2011    2582
 
BsaBI GATnn'nnATC

Cuts at:      0     498    4593 
   Size:        498    4095
 
BsaHI Gr'CG_yC

Cuts at:      0    3838    4593 
   Size:       3838     755
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121    1608    1638    4593 
   Size:         91      30    1487      30    2955

  Fragments arranged by size: 

                2955    1487      91      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     167     207     315    2861    3312    4055    4178    4593 
   Size:        167      40     108    2546     451     743     123     415

  Fragments arranged by size: 

                2546     743     451     415     167     123     108      40
            
 
BseYI C'CCAG_C

Cuts at:      0    1632    4593 
   Size:       1632    2961
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2501    4593 
   Size:       2501    2092
 
BsiHKAI G_wGCw'C

Cuts at:      0     562     882    4593 
   Size:        562     320    3711

  Fragments arranged by size: 

                3711     562     320
 
BsmI GAATG_Cn'

Cuts at:      0    1179    4349    4593 
   Size:       1179    3170     244

  Fragments arranged by size: 

                3170    1179     244
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3845    4593 
   Size:       3845     748
 
Bsp1286I G_dGCh'C

Cuts at:      0     562     799     882    3385    3649    4593 
   Size:        562     237      83    2503     264     944

  Fragments arranged by size: 

                2503     944     562     264     237      83
 
BspHI T'CATG_A

Cuts at:      0    1256    1533    1599    4593 
   Size:       1256     277      66    2994

  Fragments arranged by size: 

                2994    1256     277      66
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2907    4593 
   Size:       2907    1686
 
BsrDI GCAATG_nn'

Cuts at:      0      19    1727    2690    3365    4393    4555    4593 
   Size:         19    1708     963     675    1028     162      38

  Fragments arranged by size: 

                1708    1028     963     675     162      38      19
 
BsrGI T'GTAC_A

Cuts at:      0     425     855    4593 
   Size:        425     430    3738

  Fragments arranged by size: 

                3738     430     425
 
BssSI C'ACGA_G

Cuts at:      0    1226    4593 
   Size:       1226    3367
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1645    2077    3481    4593 
   Size:       1645     432    1404    1112

  Fragments arranged by size: 

                1645    1404    1112     432
 
BstEII G'GTnAC_C

Cuts at:      0    4088    4593 
   Size:       4088     505
 
BstYI r'GATC_y

Cuts at:      0    1594    1849    4593 
   Size:       1594     255    2744

  Fragments arranged by size: 

                2744    1594     255
 
Bsu36I CC'TnA_GG

Cuts at:      0     443    3632    4593 
   Size:        443    3189     961

  Fragments arranged by size: 

                3189     961     443
 
BtgI C'CryG_G

Cuts at:      0     227     332     722     903    3020    3824    4593 
   Size:        227     105     390     181    2117     804     769

  Fragments arranged by size: 

                2117     804     769     390     227     181     105
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2117    4593 
   Size:       2117    2476
 
BtsI GCAGTG_nn'

Cuts at:      0    1282    2379    2857    4593 
   Size:       1282    1097     478    1736

  Fragments arranged by size: 

                1736    1282    1097     478
 
DraIII CAC_nnn'GTG

Cuts at:      0     705    4593 
   Size:        705    3888
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1875    4593 
   Size:       1875    2718
 
EaeI y'GGCC_r

Cuts at:      0    4412    4593 
   Size:       4412     181
 
EarI CTCTTCn'nnn_

Cuts at:      0     225     264     403     787    1302    1700    2583    3390 
   Size:        225      39     139     384     515     398     883     807

Cuts at:   3390    4126    4593 
   Size:        736     467

  Fragments arranged by size: 

                 883     807     736     515     467     398     384     225
                 139      39
 
EcoICRI GAG'CTC

Cuts at:      0     880    4593 
   Size:        880    3713
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     191     531     803     865    1610    2296    2411 
   Size:        164      27     340     272      62     745     686     115

Cuts at:   2411    2429    2660    2744    2761    3371    3433    3493    3991 
   Size:         18     231      84      17     610      62      60     498

Cuts at:   3991    4115    4593 
   Size:        124     478

  Fragments arranged by size: 

                 745     686     610     498     478     340     272     231
                 164     124     115      84      62      62      60      27
                  18      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     892    3253    4593 
   Size:        892    2361    1340

  Fragments arranged by size: 

                2361    1340     892
 
EcoO109I rG'GnC_Cy

Cuts at:      0    4018    4593 
   Size:       4018     575
 
EcoRI G'AATT_C

Cuts at:      0    4220    4302    4491    4593 
   Size:       4220      82     189     102

  Fragments arranged by size: 

                4220     189     102      82
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1129    1368    2491    3082    4593 
   Size:       1129     239    1123     591    1511

  Fragments arranged by size: 

                1511    1129    1123     591     239
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       1      31      33      62      63      94     523     555 
   Size:          1      30       2      29       1      31     429      32

Cuts at:    555    1238    1270    1441    1473    3142    3174    3391    3423 
   Size:        683      32     171      32    1669      32     217      32

Cuts at:   3423    4593 
   Size:       1170

  Fragments arranged by size: 

                1669    1170     683     429     217     171      32      32
                  32      32      32      31      30      29       2       1
                   1
 
HincII GTy'rAC

Cuts at:      0     371    2385    3063    3410    3515    4593 
   Size:        371    2014     678     347     105    1078

  Fragments arranged by size: 

                2014    1078     678     371     347     105
 
HindIII A'AGCT_T

Cuts at:      0    1350    4593 
   Size:       1350    3243
 
HpaI GTT'AAC

Cuts at:      0     371    3063    4593 
   Size:        371    2692    1530

  Fragments arranged by size: 

                2692    1530     371
 
KpnI G_GTAC'C

Cuts at:      0     592     888    3113    4593 
   Size:        592     296    2225    1480

  Fragments arranged by size: 

                2225    1480     592     296
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     566    1863    2418    3570    4010    4593 
   Size:        445     121    1297     555    1152     440     583

  Fragments arranged by size: 

                1297    1152     583     555     445     440     121
 
MscI TGG'CCA

Cuts at:      0    4414    4593 
   Size:       4414     179
 
MslI CAynn'nnrTG

Cuts at:      0    2938    3370    4593 
   Size:       2938     432    1223

  Fragments arranged by size: 

                2938    1223     432
 
MspA1I CmG'CkG

Cuts at:      0     184    1642    2091    2331    2722    4593 
   Size:        184    1458     449     240     391    1871

  Fragments arranged by size: 

                1871    1458     449     391     240     184
 
NcoI C'CATG_G

Cuts at:      0     227    3020    3824    4593 
   Size:        227    2793     804     769

  Fragments arranged by size: 

                2793     804     769     227
 
NruI TCG'CGA

Cuts at:      0      69    4593 
   Size:         69    4524
 
NspI r_CATG'y

Cuts at:      0    4215    4593 
   Size:       4215     378
 
PasI CC'CwG_GG

Cuts at:      0    3642    4593 
   Size:       3642     951
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     549     581    4593 
   Size:        549      32    4012

  Fragments arranged by size: 

                4012     549      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    4018    4593 
   Size:       4018     575
 
PshAI GACnn'nnGTC

Cuts at:      0    3022    4593 
   Size:       3022    1571
 
PstI C_TGCA'G

Cuts at:      0     517    1042    2880    4301    4593 
   Size:        517     525    1838    1421     292

  Fragments arranged by size: 

                1838    1421     525     517     292
 
PvuII CAG'CTG

Cuts at:      0     184    1642    2091    2331    2722    4593 
   Size:        184    1458     449     240     391    1871

  Fragments arranged by size: 

                1871    1458     449     391     240     184
 
SacI G_AGCT'C

Cuts at:      0     882    4593 
   Size:        882    3711
 
SapI GCTCTTCn'nnn_

Cuts at:      0     787    2583    3390    4593 
   Size:        787    1796     807    1203

  Fragments arranged by size: 

                1796    1203     807     787
 
ScaI AGT'ACT

Cuts at:      0    2358    4593 
   Size:       2358    2235
 
SfcI C'TryA_G

Cuts at:      0     131     513     941     971    1038    2876    4297    4362 
   Size:        131     382     428      30      67    1838    1421      65

Cuts at:   4362    4593 
   Size:        231

  Fragments arranged by size: 

                1838    1421     428     382     231     131      67      65
                  30
 
SmlI C'TyrA_G

Cuts at:      0    3056    3263    3395    4100    4593 
   Size:       3056     207     132     705     493

  Fragments arranged by size: 

                3056     705     493     207     132
 
SspI AAT'ATT

Cuts at:      0    1758    2280    4593 
   Size:       1758     522    2313

  Fragments arranged by size: 

                2313    1758     522
 
StuI AGG'CCT

Cuts at:      0    1036    4593 
   Size:       1036    3557
 
StyI C'CwwG_G

Cuts at:      0     227    2000    3020    3824    4566    4593 
   Size:        227    1773    1020     804     742      27

  Fragments arranged by size: 

                1773    1020     804     742     227      27
 
TatI w'GTAC_w

Cuts at:      0     425     855    1312    2356    4593 
   Size:        425     430     457    1044    2237

  Fragments arranged by size: 

                2237    1044     457     430     425
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     472     504    1608    1640    4593 
   Size:        472      32    1104      32    2953

  Fragments arranged by size: 

                2953    1104     472      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2389    4593 
   Size:       2389    2204
 
XbaI T'CTAG_A

Cuts at:      0     118    2152    4593 
   Size:        118    2034    2441

  Fragments arranged by size: 

                2441    2034     118
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     778    3831    4593 
   Size:        778    3053     762

  Fragments arranged by size: 

                3053     778     762
 
XmnI GAAnn'nnTTC

Cuts at:      0    1493    2535    2600    4593 
   Size:       1493    1042      65    1993

  Fragments arranged by size: 

                1993    1493    1042      65
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AhdI     AlfI    AlwNI     ApoI
     BaeI     BanI    BanII     BbsI    BbvCI     BcgI     BclI    BglII
     BlpI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI   BstEII
    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I
   HincII  HindIII     HpaI     KpnI     MmeI     MscI     MslI   MspA1I
     NcoI     NruI     NspI     PasI     PpiI    PpuMI    PshAI     PstI
    PvuII     SacI     SapI     ScaI     SfcI     SmlI     SspI     StuI
     StyI     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AleI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BbeI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmtI    BsaAI
    BsaWI    BsiEI    BsiWI    BspEI    BsrBI    BsrFI   BssHII    BstBI
    BstXI  BstZ17I     ClaI    CspCI     DraI     EagI     EciI    EcoRV
     FseI     FspI    FspAI    HaeII     KasI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI    TaqII     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com