Restriction map for pF1KB7804
 (Linear) (Six-base) MAPSORT of: KIBB7804.seq  Check: 9410  from: 1  to: 1773



KIBB7804.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1720    1773 
   Size:       1720      53
 
AccI GT'mk_AC

Cuts at:      0     646    1773 
   Size:        646    1127
 
Acc65I G'GTAC_C

Cuts at:      0     886    1773 
   Size:        886     887
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319    1222    1773 
   Size:        319     903     551

  Fragments arranged by size: 

                 903     551     319
 
AfeI AGC'GCT

Cuts at:      0    1037    1773 
   Size:       1037     736
 
AflIII A'CryG_T

Cuts at:      0     692    1773 
   Size:        692    1081
 
AleI CACnn'nnGTG

Cuts at:      0    1100    1773 
   Size:       1100     673
 
AlwNI CAG_nnn'CTG

Cuts at:      0     488     685    1773 
   Size:        488     197    1088

  Fragments arranged by size: 

                1088     488     197
 
ApoI r'AATT_y

Cuts at:      0     320     565    1308    1773 
   Size:        320     245     743     465

  Fragments arranged by size: 

                 743     465     320     245
 
AvaI C'yCGr_G

Cuts at:      0     149     804    1611    1773 
   Size:        149     655     807     162

  Fragments arranged by size: 

                 807     655     162     149
 
BanI G'GyrC_C

Cuts at:      0      97     886    1094    1773 
   Size:         97     789     208     679

  Fragments arranged by size: 

                 789     679     208      97
 
BanII G_rGCy'C

Cuts at:      0     156     732    1773 
   Size:        156     576    1041

  Fragments arranged by size: 

                1041     576     156
 
BbeI G_GCGC'C

Cuts at:      0     101    1773 
   Size:        101    1672
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1462    1773 
   Size:       1462     311
 
BclI T'GATC_A

Cuts at:      0     839     911    1773 
   Size:        839      72     862

  Fragments arranged by size: 

                 862     839      72
 
BglI GCCn_nnn'nGGC

Cuts at:      0     121    1773 
   Size:        121    1652
 
BlpI GC'TnA_GC

Cuts at:      0    1524    1773 
   Size:       1524     249
 
BmgBI CAC'GTC

Cuts at:      0      86    1773 
   Size:         86    1687
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     478     546    1439    1773 
   Size:        478      68     893     334

  Fragments arranged by size: 

                 893     478     334      68
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     921    1773 
   Size:        921     852
 
Bpu10I CC'TnA_GC

Cuts at:      0    1109    1773 
   Size:       1109     664
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1368    1773 
   Size:       1368     405
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1650    1773 
   Size:       1650     123
 
BsaAI yAC'GTr

Cuts at:      0     381     616    1410    1773 
   Size:        381     235     794     363

  Fragments arranged by size: 

                 794     381     363     235
 
BsaHI Gr'CG_yC

Cuts at:      0      98     113    1773 
   Size:         98      15    1660

  Fragments arranged by size: 

                1660      98      15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1429    1459    1773 
   Size:       1429      30     314

  Fragments arranged by size: 

                1429     314      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     908     935    1566    1599    1773 
   Size:        908      27     631      33     174

  Fragments arranged by size: 

                 908     631     174      33      27
 
BseYI C'CCAG_C

Cuts at:      0      57    1427    1773 
   Size:         57    1370     346

  Fragments arranged by size: 

                1370     346      57
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501     955    1698    1773 
   Size:        501     454     743      75

  Fragments arranged by size: 

                 743     501     454      75
 
BsiEI CG_ry'CG

Cuts at:      0    1214    1773 
   Size:       1214     559
 
BsiHKAI G_wGCw'C

Cuts at:      0    1420    1773 
   Size:       1420     353
 
BsmI GAATG_Cn'

Cuts at:      0     606    1773 
   Size:        606    1167
 
Bsp1286I G_dGCh'C

Cuts at:      0     156     732    1420    1773 
   Size:        156     576     688     353

  Fragments arranged by size: 

                 688     576     353     156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1720    1773 
   Size:       1720      53
 
BsrBI CCG'CTC

Cuts at:      0     134    1210    1773 
   Size:        134    1076     563

  Fragments arranged by size: 

                1076     563     134
 
BsrDI GCAATG_nn'

Cuts at:      0     357    1217    1490    1773 
   Size:        357     860     273     283

  Fragments arranged by size: 

                 860     357     283     273
 
BsrFI r'CCGG_y

Cuts at:      0     329    1773 
   Size:        329    1444
 
BsrGI T'GTAC_A

Cuts at:      0    1232    1773 
   Size:       1232     541
 
BssSI C'ACGA_G

Cuts at:      0    1455    1773 
   Size:       1455     318
 
BstEII G'GTnAC_C

Cuts at:      0    1102    1773 
   Size:       1102     671
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      31    1773 
   Size:         31    1742
 
BstYI r'GATC_y

Cuts at:      0     534    1149    1773 
   Size:        534     615     624

  Fragments arranged by size: 

                 624     615     534
 
BtgI C'CryG_G

Cuts at:      0     242    1568    1773 
   Size:        242    1326     205

  Fragments arranged by size: 

                1326     242     205
 
ClaI AT'CG_AT

Cuts at:      0    1055    1773 
   Size:       1055     718
 
EaeI y'GGCC_r

Cuts at:      0     666    1211    1328    1773 
   Size:        666     545     117     445

  Fragments arranged by size: 

                 666     545     445     117
 
EagI C'GGCC_G

Cuts at:      0    1211    1773 
   Size:       1211     562
 
EarI CTCTTCn'nnn_

Cuts at:      0      44    1198    1657    1773 
   Size:         44    1154     459     116

  Fragments arranged by size: 

                1154     459     116      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     738    1773 
   Size:        738    1035
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     921    1222    1773 
   Size:        319     602     301     551

  Fragments arranged by size: 

                 602     551     319     301
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1227    1773 
   Size:       1227     546
 
EcoRI G'AATT_C

Cuts at:      0     320    1308    1773 
   Size:        320     988     465

  Fragments arranged by size: 

                 988     465     320
 
EcoRV GAT'ATC

Cuts at:      0    1748    1773 
   Size:       1748      25
 
HaeII r_GCGC'y

Cuts at:      0     101    1039    1773 
   Size:        101     938     734

  Fragments arranged by size: 

                 938     734     101
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     620     652     903     930     935     962    1749    1773 
   Size:        620      32     251      27       5      27     787      24

  Fragments arranged by size: 

                 787     620     251      32      27      27      24       5
            
 
KasI G'GCGC_C

Cuts at:      0      97    1773 
   Size:         97    1676
 
KpnI G_GTAC'C

Cuts at:      0     890    1773 
   Size:        890     883
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     218    1619    1773 
   Size:         48     170    1401     154

  Fragments arranged by size: 

                1401     170     154      48
 
MscI TGG'CCA

Cuts at:      0     668    1773 
   Size:        668    1105
 
MslI CAynn'nnrTG

Cuts at:      0     931    1100    1459    1773 
   Size:        931     169     359     314

  Fragments arranged by size: 

                 931     359     314     169
 
MspA1I CmG'CkG

Cuts at:      0      57      65     488    1528    1773 
   Size:         57       8     423    1040     245

  Fragments arranged by size: 

                1040     423     245      57       8
 
NarI GG'CG_CC

Cuts at:      0      98    1773 
   Size:         98    1675
 
NcoI C'CATG_G

Cuts at:      0    1568    1773 
   Size:       1568     205
 
NspI r_CATG'y

Cuts at:      0     624     696     778    1773 
   Size:        624      72      82     995

  Fragments arranged by size: 

                 995     624      82      72
 
PciI A'CATG_T

Cuts at:      0     692    1773 
   Size:        692    1081
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243     639     675    1773 
   Size:        243     396      36    1098

  Fragments arranged by size: 

                1098     396     243      36
 
PmlI CAC'GTG

Cuts at:      0     616    1773 
   Size:        616    1157
 
PstI C_TGCA'G

Cuts at:      0    1603    1773 
   Size:       1603     170
 
PvuII CAG'CTG

Cuts at:      0    1528    1773 
   Size:       1528     245
 
SapI GCTCTTCn'nnn_

Cuts at:      0      44    1773 
   Size:         44    1729
 
SfcI C'TryA_G

Cuts at:      0     345    1599    1773 
   Size:        345    1254     174

  Fragments arranged by size: 

                1254     345     174
 
SfoI GGC'GCC

Cuts at:      0      99    1773 
   Size:         99    1674
 
SmaI CCC'GGG

Cuts at:      0     151    1773 
   Size:        151    1622
 
SmlI C'TyrA_G

Cuts at:      0    1383    1773 
   Size:       1383     390
 
SrfI GCCC'GGGC

Cuts at:      0     151    1773 
   Size:        151    1622
 
StyI C'CwwG_G

Cuts at:      0    1568    1773 
   Size:       1568     205
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1272    1773 
   Size:       1272     501
 
TatI w'GTAC_w

Cuts at:      0    1232    1773 
   Size:       1232     541
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1566    1773 
   Size:       1566     207
 
XmaI C'CCGG_G

Cuts at:      0     149    1773 
   Size:        149    1624
 
XmnI GAAnn'nnTTC

Cuts at:      0     568    1196    1312    1773 
   Size:        568     628     116     461

  Fragments arranged by size: 

                 628     568     461     116
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AleI    AlwNI
     ApoI     AvaI     BanI    BanII     BbeI    BciVI     BclI     BglI
     BlpI    BmgBI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstEII
    BstXI    BstYI     BtgI     ClaI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PciI
    PflMI     PmlI     PstI    PvuII     SapI     SfcI     SfoI     SmaI
     SmlI     SrfI     StyI    TaqII     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AhdI     AlfI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
    BbvCI     BcgI    BfrBI    BglII Bme1580I     BmrI     BmtI    BsaBI
    BsaWI    BsiWI    BsmBI    BspEI    BspHI   BssHII   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com