Restriction map for pF1KB9409
 (Linear) (Six-base) MAPSORT of: KIBB9409.seq  Check: 2649  from: 1  to: 867



KIBB9409.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AfeI AGC'GCT

Cuts at:      0     106     867 
   Size:        106     761
 
AleI CACnn'nnGTG

Cuts at:      0     715     867 
   Size:        715     152
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      58     867 
   Size:         58     809
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     405     867 
   Size:         87     318     462

  Fragments arranged by size: 

                 462     318      87
 
BamHI G'GATC_C

Cuts at:      0     802     867 
   Size:        802      65
 
BanII G_rGCy'C

Cuts at:      0     641     664     867 
   Size:        641      23     203

  Fragments arranged by size: 

                 641     203      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     219     867 
   Size:        219     648
 
BmgBI CAC'GTC

Cuts at:      0     717     849     867 
   Size:        717     132      18

  Fragments arranged by size: 

                 717     132      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     430     625     867 
   Size:        211     219     195     242

  Fragments arranged by size: 

                 242     219     211     195
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     334     867 
   Size:        334     533
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     352     382     505     535     867 
   Size:         25     327      30     123      30     332

  Fragments arranged by size: 

                 332     327     123      30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     115     455     751     867 
   Size:        115     340     296     116

  Fragments arranged by size: 

                 340     296     116     115
 
BseYI C'CCAG_C

Cuts at:      0     265     429     503     867 
   Size:        265     164      74     364

  Fragments arranged by size: 

                 364     265     164      74
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     867 
   Size:        160     707
 
BsiEI CG_ry'CG

Cuts at:      0      72     867 
   Size:         72     795
 
BsiHKAI G_wGCw'C

Cuts at:      0     124     485     664     761     867 
   Size:        124     361     179      97     106

  Fragments arranged by size: 

                 361     179     124     106      97
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      59     867 
   Size:         59     808
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     485     641     664     761     867 
   Size:        124     361     156      23      97     106

  Fragments arranged by size: 

                 361     156     124     106      97      23
 
BspHI T'CATG_A

Cuts at:      0     468     867 
   Size:        468     399
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65      95     867 
   Size:         65      30     772

  Fragments arranged by size: 

                 772      65      30
 
BsrDI GCAATG_nn'

Cuts at:      0     551     867 
   Size:        551     316
 
BssSI C'ACGA_G

Cuts at:      0      16     867 
   Size:         16     851
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      87     489     867 
   Size:         87     402     378

  Fragments arranged by size: 

                 402     378      87
 
BstYI r'GATC_y

Cuts at:      0     802     867 
   Size:        802      65
 
Bsu36I CC'TnA_GG

Cuts at:      0     398     867 
   Size:        398     469
 
BtgI C'CryG_G

Cuts at:      0     356     867 
   Size:        356     511
 
BtsI GCAGTG_nn'

Cuts at:      0     124     867 
   Size:        124     743
 
DraIII CAC_nnn'GTG

Cuts at:      0      21     867 
   Size:         21     846
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      73     282     867 
   Size:         73     209     585

  Fragments arranged by size: 

                 585     209      73
 
EaeI y'GGCC_r

Cuts at:      0     176     236     867 
   Size:        176      60     631

  Fragments arranged by size: 

                 631     176      60
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     867 
   Size:        215     652
 
EcoICRI GAG'CTC

Cuts at:      0     662     867 
   Size:        662     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     211     430     625     867 
   Size:        211     219     195     242

  Fragments arranged by size: 

                 242     219     211     195
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270     867 
   Size:        270     597
 
EcoRV GAT'ATC

Cuts at:      0     858     867 
   Size:        858       9
 
FspI TGC'GCA

Cuts at:      0     166     493     867 
   Size:        166     327     374

  Fragments arranged by size: 

                 374     327     166
 
HaeII r_GCGC'y

Cuts at:      0     108     867 
   Size:        108     759
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     505     537     614     646     867 
   Size:        505      32      77      32     221

  Fragments arranged by size: 

                 505     221      77      32      32
 
HindIII A'AGCT_T

Cuts at:      0     731     867 
   Size:        731     136
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     791     867 
   Size:        177     614      76

  Fragments arranged by size: 

                 614     177      76
 
MscI TGG'CCA

Cuts at:      0     178     238     867 
   Size:        178      60     629

  Fragments arranged by size: 

                 629     178      60
 
MslI CAynn'nnrTG

Cuts at:      0     184     715     867 
   Size:        184     531     152

  Fragments arranged by size: 

                 531     184     152
 
MspA1I CmG'CkG

Cuts at:      0     351     358     457     510     867 
   Size:        351       7      99      53     357

  Fragments arranged by size: 

                 357     351      99      53       7
 
NspI r_CATG'y

Cuts at:      0     165     318     541     867 
   Size:        165     153     223     326

  Fragments arranged by size: 

                 326     223     165     153
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      21     867 
   Size:         21     846
 
PpuMI rG'GwC_Cy

Cuts at:      0     270     867 
   Size:        270     597
 
PstI C_TGCA'G

Cuts at:      0      83     776     867 
   Size:         83     693      91

  Fragments arranged by size: 

                 693      91      83
 
PvuII CAG'CTG

Cuts at:      0     457     867 
   Size:        457     410
 
RsrII CG'GwC_CG

Cuts at:      0      30     867 
   Size:         30     837
 
SacI G_AGCT'C

Cuts at:      0     664     867 
   Size:        664     203
 
SacII CC_GC'GG

Cuts at:      0     359     867 
   Size:        359     508
 
SanDI GG'GwC_CC

Cuts at:      0     270     867 
   Size:        270     597
 
SfcI C'TryA_G

Cuts at:      0      79     772     842     867 
   Size:         79     693      70      25

  Fragments arranged by size: 

                 693      79      70      25
 
SphI G_CATG'C

Cuts at:      0     541     867 
   Size:        541     326
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292     867 
   Size:        292     575
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     196     228     352     384     867 
   Size:         27     169      32     124      32     483

  Fragments arranged by size: 

                 483     169     124      32      32      27
 
Tth111I GACn'n_nGTC

Cuts at:      0      28      73     867 
   Size:         28      45     794

  Fragments arranged by size: 

                 794      45      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     186     246     714     867 
   Size:        186      60     468     153

  Fragments arranged by size: 

                 468     186     153      60
 

 Enzymes that do cut:

     AfeI     AleI     AlfI    AlwNI    BamHI    BanII     BbsI    BmgBI
     BpmI     BsaI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BssSI   BstAPI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FspI    HaeII    Hin4I  HindIII     MmeI
     MscI     MslI   MspA1I     NspI    PflMI    PpuMI     PstI    PvuII
    RsrII     SacI    SacII    SanDI     SfcI     SphI    TaqII     TstI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiWI     BsmI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI     EagI
     EciI    EcoNI    EcoRI     FalI     FseI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI     TatI     XbaI     XhoI     XmaI
     XmnI     ZraI
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