Restriction map for pF1KB9397
 (Circular) (Six-base) MAPSORT of: pF1KB9397.seq  Check: 5135  from: 1  to: 5063



pF1KB9397  5063 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    927     927 
   Size:       5063
 
AccI GT'mk_AC

Cuts at:   2101    2101 
   Size:       5063
 
Acc65I G'GTAC_C

Cuts at:   2079    2079 
   Size:       5063
 
AclI AA'CG_TT

Cuts at:   4903    4903 
   Size:       5063
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    311     335     399     490    2888    3320    3673    4335     311 
   Size:         24      64      91    2398     432     353     662    1039

  Fragments arranged by size: 

                2398    1039     662     432     353      91      64      24
            
 
AflIII A'CryG_T

Cuts at:   1271    2415    4215    4496    1271 
   Size:       1144    1800     281    1838

  Fragments arranged by size: 

                1838    1800    1144     281
 
AlwNI CAG_nnn'CTG

Cuts at:    298    2368    3806     298 
   Size:       2070    1438    1555

  Fragments arranged by size: 

                2070    1555    1438
 
ApaLI G'TGCA_C

Cuts at:   3901    3901 
   Size:       5063
 
ApoI r'AATT_y

Cuts at:   1009    1326    2067    4416    4672    1009 
   Size:        317     741    2349     256    1400

  Fragments arranged by size: 

                2349    1400     741     317     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5063
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5063
 
AvaI C'yCGr_G

Cuts at:    929    1214    2083     929 
   Size:        285     869    3909

  Fragments arranged by size: 

                3909     869     285
 
AvrII C'CTAG_G

Cuts at:   1966    1966 
   Size:       5063
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    502     535     502 
   Size:         33    5030
 
BamHI G'GATC_C

Cuts at:   1838    2088    1838 
   Size:        250    4813
 
BanI G'GyrC_C

Cuts at:    658    2079    2493    2741    2776     658 
   Size:       1421     414     248      35    2945

  Fragments arranged by size: 

                2945    1421     414     248      35
 
BanII G_rGCy'C

Cuts at:    875    2077    3107     875 
   Size:       1202    1030    2831

  Fragments arranged by size: 

                2831    1202    1030
 
BbeI G_GCGC'C

Cuts at:   2497    2745    2497 
   Size:        248    4815
 
BbsI GAAGACnn'nnnn_

Cuts at:    464     550     566     464 
   Size:         86      16    4961

  Fragments arranged by size: 

                4961      86      16
 
BciVI GTATCCnnnnn_n'

Cuts at:    805    2961    4017    5063     805 
   Size:       2156    1056    1046     805

  Fragments arranged by size: 

                2156    1056    1046     805
 
BclI T'GATC_A

Cuts at:    695    2583     695 
   Size:       1888    3175
 
BglI GCCn_nnn'nGGC

Cuts at:    378     378 
   Size:       5063
 
BglII A'GATC_T

Cuts at:    910    1562    2578    4615     910 
   Size:        652    1016    2037    1358

  Fragments arranged by size: 

                2037    1358    1016     652
 
BlpI GC'TnA_GC

Cuts at:   1817    2175    1817 
   Size:        358    4705
 
Bme1580I G_kGCm'C

Cuts at:    663    2688    2781    3905     663 
   Size:       2025      93    1124    1821

  Fragments arranged by size: 

                2025    1821    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1844    2275    2356    2691    4835    1844 
   Size:        431      81     335    2144    2072

  Fragments arranged by size: 

                2144    2072     431     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1082    1706    3430    1082 
   Size:        624    1724    2715

  Fragments arranged by size: 

                2715    1724     624
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1879    1879 
   Size:       5063
 
Bpu10I CC'TnA_GC

Cuts at:   1796    1796 
   Size:       5063
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1077    1684    2012    2239    3118    3832    4130    1077 
   Size:        607     328     227     879     714     298    2010

  Fragments arranged by size: 

                2010     879     714     607     328     298     227
 
BsaI GGTCTCn'nnnn_

Cuts at:    277    4767     277 
   Size:       4490     573
 
BsaAI yAC'GTr

Cuts at:   2416    2416 
   Size:       5063
 
BsaBI GATnn'nnATC

Cuts at:      6    1151    2577    4614       6 
   Size:       1145    1426    2037     455

  Fragments arranged by size: 

                2037    1426    1145     455
 
BsaHI Gr'CG_yC

Cuts at:    112    1045    2494    2742    3444    4940     112 
   Size:        933    1449     248     702    1496     235

  Fragments arranged by size: 

                1496    1449     933     702     248     235
 
BsaWI w'CCGG_w

Cuts at:   1841    2252    2773    3451    3862    4009    4283    1841 
   Size:        411     521     678     411     147     274    2621

  Fragments arranged by size: 

                2621     678     521     411     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2002    2032    2002 
   Size:         30    5033
 
BseYI C'CCAG_C

Cuts at:   2488    3246    3911    4319    2488 
   Size:        758     665     408    3232

  Fragments arranged by size: 

                3232     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    905     905 
   Size:       5063
 
BsiEI CG_ry'CG

Cuts at:    104    2605    2651    3881     104 
   Size:       2501      46    1230    1286

  Fragments arranged by size: 

                2501    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1960    2077    2855    3045    3905    1960 
   Size:        117     778     190     860    3118

  Fragments arranged by size: 

                3118     860     778     190     117
 
BsmI GAATG_Cn'

Cuts at:    257     257 
   Size:       5063
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1967    1967 
   Size:       5063
 
Bsp1286I G_dGCh'C

Cuts at:    663     875    1960    2077    2688    2781    2855    3045    3107 
   Size:        212    1085     117     611      93      74     190      62

Cuts at:   3107    3905     663 
   Size:        798    1821

  Fragments arranged by size: 

                1821    1085     798     611     212     190     117      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   1841    1841 
   Size:       5063
 
BspHI T'CATG_A

Cuts at:   3495    3495 
   Size:       5063
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    306     927    2113    2327    2629    3010     306 
   Size:        621    1186     214     302     381    2359

  Fragments arranged by size: 

                2359    1186     621     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1869    3355    3409    4893    5058      50 
   Size:       1819    1486      54    1484     165      55

  Fragments arranged by size: 

                1819    1486    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    190    1562    2975     190 
   Size:       1372    1413    2278

  Fragments arranged by size: 

                2278    1413    1372
 
BsrFI r'CCGG_y

Cuts at:   3061    3242    3061 
   Size:        181    4882
 
BssSI C'ACGA_G

Cuts at:   1693    3334    4042    1693 
   Size:       1641     708    2714

  Fragments arranged by size: 

                2714    1641     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1788    2440    1788 
   Size:        652    4411
 
BstBI TT'CG_AA

Cuts at:   3424    3424 
   Size:       5063
 
BstYI r'GATC_y

Cuts at:      1     910    1347    1562    1838    2088    2555    2578    2913 
   Size:        909     437     215     276     250     467      23     335

Cuts at:   2913    3159    3465    3477    3563    4615       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                1052     909     467     449     437     335     306     276
                 250     246     215      86      23      12
 
BtgI C'CryG_G

Cuts at:    107    1933     107 
   Size:       1826    3237
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    995    3013    3194    3357     995 
   Size:       2018     181     163    2701

  Fragments arranged by size: 

                2701    2018     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1413    2306    1413 
   Size:        893    4170
 
DraI TTT'AAA

Cuts at:   2062    2062 
   Size:       5063
 
DraIII CAC_nnn'GTG

Cuts at:   1388    1388 
   Size:       5063
 
DrdI GACnn_nn'nnGTC

Cuts at:    568    2769    4113     568 
   Size:       2201    1344    1518

  Fragments arranged by size: 

                2201    1518    1344
 
EaeI y'GGCC_r

Cuts at:    826    1880    2648    2822    3213    3240    4585     826 
   Size:       1054     768     174     391      27    1345    1304

  Fragments arranged by size: 

                1345    1304    1054     768     391     174      27
 
EagI C'GGCC_G

Cuts at:   2648    2648 
   Size:       5063
 
EarI CTCTTCn'nnn_

Cuts at:    348    1624    1742    3086    3296     348 
   Size:       1276     118    1344     210    2115

  Fragments arranged by size: 

                2115    1344    1276     210     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    157    3271    4013    4159    4873     157 
   Size:       3114     742     146     714     347

  Fragments arranged by size: 

                3114     742     714     347     146
 
EcoICRI GAG'CTC

Cuts at:   2075    2075 
   Size:       5063
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    311     335     399     490    1879    2888    3320    3673    4335 
   Size:         24      64      91    1389    1009     432     353     662

Cuts at:   4335     311 
   Size:       1039

  Fragments arranged by size: 

                1389    1039    1009     662     432     353      91      64
                  24
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1965    1965 
   Size:       5063
 
EcoO109I rG'GnC_Cy

Cuts at:    295    2202     295 
   Size:       1907    3156
 
EcoRI G'AATT_C

Cuts at:   2067    2067 
   Size:       5063
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    929    2053    3082     929 
   Size:       1124    1029    2910

  Fragments arranged by size: 

                2910    1124    1029
 
FspI TGC'GCA

Cuts at:   2844    2844 
   Size:       5063
 
HaeII r_GCGC'y

Cuts at:   2497    2745    3975    4746    2497 
   Size:        248    1230     771    2814

  Fragments arranged by size: 

                2814    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2905    2935    2937    2967    2905 
   Size:         30       2      30    5001

  Fragments arranged by size: 

                5001      30      30       2
 
HincII GTy'rAC

Cuts at:   1265    2102    1265 
   Size:        837    4226
 
HindIII A'AGCT_T

Cuts at:   1225    1225 
   Size:       5063
 
HpaI GTT'AAC

Cuts at:   1265    1265 
   Size:       5063
 
KasI G'GCGC_C

Cuts at:   2493    2741    2493 
   Size:        248    4815
 
KpnI G_GTAC'C

Cuts at:   2083    2083 
   Size:       5063
 
MluI A'CGCG_T

Cuts at:   4496    4496 
   Size:       5063
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    140     453     920    1388    3822    4006     140 
   Size:        313     467     468    2434     184    1197

  Fragments arranged by size: 

                2434    1197     468     467     313     184
 
MscI TGG'CCA

Cuts at:    828    1882    2824     828 
   Size:       1054     942    3067

  Fragments arranged by size: 

                3067    1054     942
 
MslI CAynn'nnrTG

Cuts at:    656    1250    3052    3179     656 
   Size:        594    1802     127    2540

  Fragments arranged by size: 

                2540    1802     594     127
 
MspA1I CmG'CkG

Cuts at:    720    2174    2488    2848    3632    3877     720 
   Size:       1454     314     360     784     245    1906

  Fragments arranged by size: 

                1906    1454     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   3244    3244 
   Size:       5063
 
NarI GG'CG_CC

Cuts at:   2494    2742    2494 
   Size:        248    4815
 
NcoI C'CATG_G

Cuts at:    107    1933     107 
   Size:       1826    3237
 
NdeI CA'TA_TG

Cuts at:    965     965 
   Size:       5063
 
NgoMIV G'CCGG_C

Cuts at:   3242    3242 
   Size:       5063
 
NspI r_CATG'y

Cuts at:    158    2116    2616    4219     158 
   Size:       1958     500    1603    1002

  Fragments arranged by size: 

                1958    1603    1002     500
 
PciI A'CATG_T

Cuts at:   4215    4215 
   Size:       5063
 
PmeI GTTT'AAAC

Cuts at:   2062    2062 
   Size:       5063
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3509    3541    3509 
   Size:         32    5031
 
PpuMI rG'GwC_Cy

Cuts at:    295     295 
   Size:       5063
 
PsiI TTA'TAA

Cuts at:   1333    1333 
   Size:       5063
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    494     526     494 
   Size:         32    5031
 
PstI C_TGCA'G

Cuts at:    668    2110    2795     668 
   Size:       1442     685    2936

  Fragments arranged by size: 

                2936    1442     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5063
 
PvuII CAG'CTG

Cuts at:   2488    2848    2488 
   Size:        360    4703
 
RsrII CG'GwC_CG

Cuts at:   3258    3258 
   Size:       5063
 
SacI G_AGCT'C

Cuts at:   2077    2077 
   Size:       5063
 
SalI G'TCGA_C

Cuts at:   2100    2100 
   Size:       5063
 
SapI GCTCTTCn'nnn_

Cuts at:   3086    3296    3086 
   Size:        210    4853
 
SbfI CC_TGCA'GG

Cuts at:    668    2110     668 
   Size:       1442    3621
 
ScaI AGT'ACT

Cuts at:    753    4580     753 
   Size:       3827    1236
 
SfcI C'TryA_G

Cuts at:     33     664     839    1004    2106    2791    3759    3950      33 
   Size:        631     175     165    1102     685     968     191    1146

  Fragments arranged by size: 

                1146    1102     968     685     631     191     175     165
            
 
SfoI GGC'GCC

Cuts at:   2495    2743    2495 
   Size:        248    4815
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5063
 
SmaI CCC'GGG

Cuts at:    931    2085     931 
   Size:       1154    3909
 
SmlI C'TyrA_G

Cuts at:   1056    1663    2027    2218    3133    3847    4109    1056 
   Size:        607     364     191     915     714     262    2010

  Fragments arranged by size: 

                2010     915     714     607     364     262     191
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5063
 
SphI G_CATG'C

Cuts at:   2116    2616    2116 
   Size:        500    4563
 
StuI AGG'CCT

Cuts at:   1198    1648    1198 
   Size:        450    4613
 
StyI C'CwwG_G

Cuts at:    107    1933    1966    2197    2551     107 
   Size:       1826      33     231     354    2619

  Fragments arranged by size: 

                2619    1826     354     231      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    718    2646    2779    3238    3447     718 
   Size:       1928     133     459     209    2334

  Fragments arranged by size: 

                2334    1928     459     209     133
 
TatI w'GTAC_w

Cuts at:    157     466     725     751    1943    4578     157 
   Size:        309     259      26    1192    2635     642

  Fragments arranged by size: 

                2635    1192     642     309     259      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1836    1868    4580    4612    1836 
   Size:         32    2712      32    2287

  Fragments arranged by size: 

                2712    2287      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2860    2860 
   Size:       5063
 
XbaI T'CTAG_A

Cuts at:    492    2094     492 
   Size:       1602    3461
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2558    2558 
   Size:       5063
 
XmaI C'CCGG_G

Cuts at:    929    2083     929 
   Size:       1154    3909
 
XmnI GAAnn'nnTTC

Cuts at:    912    1715     912 
   Size:        803    4260
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PciI     PmeI     PpiI    PpuMI     PsiI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BseRI
    BsiWI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI    EcoRV     FseI    FspAI     MfeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI   PspOMI
    PspXI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     ZraI
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