Restriction map for pF1KB9397
 (Linear) (Six-base) MAPSORT of: KIBB9397.seq  Check: 4327  from: 1  to: 1950



KIBB9397.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     819    1950 
   Size:        819    1131
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     227     291     382    1950 
   Size:        203      24      64      91    1568

  Fragments arranged by size: 

                1568     203      91      64      24
 
AflIII A'CryG_T

Cuts at:      0    1163    1950 
   Size:       1163     787
 
AlwNI CAG_nnn'CTG

Cuts at:      0     190    1950 
   Size:        190    1760
 
ApoI r'AATT_y

Cuts at:      0     901    1218    1950 
   Size:        901     317     732

  Fragments arranged by size: 

                 901     732     317
 
AvaI C'yCGr_G

Cuts at:      0     821    1106    1950 
   Size:        821     285     844

  Fragments arranged by size: 

                 844     821     285
 
AvrII C'CTAG_G

Cuts at:      0    1858    1950 
   Size:       1858      92
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     394     427    1950 
   Size:        394      33    1523

  Fragments arranged by size: 

                1523     394      33
 
BamHI G'GATC_C

Cuts at:      0    1730    1950 
   Size:       1730     220
 
BanI G'GyrC_C

Cuts at:      0     550    1950 
   Size:        550    1400
 
BanII G_rGCy'C

Cuts at:      0     767    1950 
   Size:        767    1183
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     356     442     458    1950 
   Size:        356      86      16    1492

  Fragments arranged by size: 

                1492     356      86      16
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     697    1950 
   Size:        697    1253
 
BclI T'GATC_A

Cuts at:      0     587    1950 
   Size:        587    1363
 
BglI GCCn_nnn'nGGC

Cuts at:      0     270    1950 
   Size:        270    1680
 
BglII A'GATC_T

Cuts at:      0     802    1454    1950 
   Size:        802     652     496

  Fragments arranged by size: 

                 802     652     496
 
BlpI GC'TnA_GC

Cuts at:      0    1709    1950 
   Size:       1709     241
 
Bme1580I G_kGCm'C

Cuts at:      0     555    1950 
   Size:        555    1395
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1736    1950 
   Size:       1736     214
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     974    1598    1950 
   Size:        974     624     352

  Fragments arranged by size: 

                 974     624     352
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1771    1950 
   Size:       1771     179
 
Bpu10I CC'TnA_GC

Cuts at:      0    1688    1950 
   Size:       1688     262
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     969    1576    1904    1950 
   Size:        969     607     328      46

  Fragments arranged by size: 

                 969     607     328      46
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     169    1950 
   Size:        169    1781
 
BsaBI GATnn'nnATC

Cuts at:      0    1043    1950 
   Size:       1043     907
 
BsaHI Gr'CG_yC

Cuts at:      0       4     937    1950 
   Size:          4     933    1013

  Fragments arranged by size: 

                1013     933       4
 
BsaWI w'CCGG_w

Cuts at:      0    1733    1950 
   Size:       1733     217
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1894    1924    1950 
   Size:       1894      30      26

  Fragments arranged by size: 

                1894      30      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     797    1950 
   Size:        797    1153
 
BsiHKAI G_wGCw'C

Cuts at:      0    1852    1950 
   Size:       1852      98
 
BsmI GAATG_Cn'

Cuts at:      0     149    1950 
   Size:        149    1801
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1859    1950 
   Size:       1859      91
 
Bsp1286I G_dGCh'C

Cuts at:      0     555     767    1852    1950 
   Size:        555     212    1085      98

  Fragments arranged by size: 

                1085     555     212      98
 
BspEI T'CCGG_A

Cuts at:      0    1733    1950 
   Size:       1733     217
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     198     819    1950 
   Size:        198     621    1131

  Fragments arranged by size: 

                1131     621     198
 
BsrBI CCG'CTC

Cuts at:      0    1761    1950 
   Size:       1761     189
 
BsrDI GCAATG_nn'

Cuts at:      0      82    1454    1950 
   Size:         82    1372     496

  Fragments arranged by size: 

                1372     496      82
 
BssSI C'ACGA_G

Cuts at:      0    1585    1950 
   Size:       1585     365
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1680    1950 
   Size:       1680     270
 
BstYI r'GATC_y

Cuts at:      0     802    1239    1454    1730    1950 
   Size:        802     437     215     276     220

  Fragments arranged by size: 

                 802     437     276     220     215
 
BtgI C'CryG_G

Cuts at:      0    1825    1950 
   Size:       1825     125
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     887    1950 
   Size:        887    1063
 
BtsI GCAGTG_nn'

Cuts at:      0    1305    1950 
   Size:       1305     645
 
DraIII CAC_nnn'GTG

Cuts at:      0    1280    1950 
   Size:       1280     670
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     460    1950 
   Size:        460    1490
 
EaeI y'GGCC_r

Cuts at:      0     718    1772    1950 
   Size:        718    1054     178

  Fragments arranged by size: 

                1054     718     178
 
EarI CTCTTCn'nnn_

Cuts at:      0     240    1516    1634    1950 
   Size:        240    1276     118     316

  Fragments arranged by size: 

                1276     316     240     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      49    1950 
   Size:         49    1901
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     227     291     382    1771    1950 
   Size:        203      24      64      91    1389     179

  Fragments arranged by size: 

                1389     203     179      91      64      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1857    1950 
   Size:       1857      93
 
EcoO109I rG'GnC_Cy

Cuts at:      0     187    1950 
   Size:        187    1763
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     821    1945    1950 
   Size:        821    1124       5

  Fragments arranged by size: 

                1124     821       5
 
HincII GTy'rAC

Cuts at:      0    1157    1950 
   Size:       1157     793
 
HindIII A'AGCT_T

Cuts at:      0    1117    1950 
   Size:       1117     833
 
HpaI GTT'AAC

Cuts at:      0    1157    1950 
   Size:       1157     793
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     345     812    1280    1950 
   Size:         32     313     467     468     670

  Fragments arranged by size: 

                 670     468     467     313      32
 
MscI TGG'CCA

Cuts at:      0     720    1774    1950 
   Size:        720    1054     176

  Fragments arranged by size: 

                1054     720     176
 
MslI CAynn'nnrTG

Cuts at:      0     548    1142    1950 
   Size:        548     594     808

  Fragments arranged by size: 

                 808     594     548
 
MspA1I CmG'CkG

Cuts at:      0     612    1950 
   Size:        612    1338
 
NcoI C'CATG_G

Cuts at:      0    1825    1950 
   Size:       1825     125
 
NdeI CA'TA_TG

Cuts at:      0     857    1950 
   Size:        857    1093
 
NspI r_CATG'y

Cuts at:      0      50    1950 
   Size:         50    1900
 
PpuMI rG'GwC_Cy

Cuts at:      0     187    1950 
   Size:        187    1763
 
PsiI TTA'TAA

Cuts at:      0    1225    1950 
   Size:       1225     725
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     386     418    1950 
   Size:        386      32    1532

  Fragments arranged by size: 

                1532     386      32
 
PstI C_TGCA'G

Cuts at:      0     560    1950 
   Size:        560    1390
 
SbfI CC_TGCA'GG

Cuts at:      0     560    1950 
   Size:        560    1390
 
ScaI AGT'ACT

Cuts at:      0     645    1950 
   Size:        645    1305
 
SfcI C'TryA_G

Cuts at:      0     556     731     896    1950 
   Size:        556     175     165    1054

  Fragments arranged by size: 

                1054     556     175     165
 
SmaI CCC'GGG

Cuts at:      0     823    1950 
   Size:        823    1127
 
SmlI C'TyrA_G

Cuts at:      0     948    1555    1919    1950 
   Size:        948     607     364      31

  Fragments arranged by size: 

                 948     607     364      31
 
StuI AGG'CCT

Cuts at:      0    1090    1540    1950 
   Size:       1090     450     410

  Fragments arranged by size: 

                1090     450     410
 
StyI C'CwwG_G

Cuts at:      0    1825    1858    1950 
   Size:       1825      33      92

  Fragments arranged by size: 

                1825      92      33
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     610    1950 
   Size:        610    1340
 
TatI w'GTAC_w

Cuts at:      0      49     358     617     643    1835    1950 
   Size:         49     309     259      26    1192     115

  Fragments arranged by size: 

                1192     309     259     115      49      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1728    1760    1950 
   Size:       1728      32     190

  Fragments arranged by size: 

                1728     190      32
 
XbaI T'CTAG_A

Cuts at:      0     384    1950 
   Size:        384    1566
 
XmaI C'CCGG_G

Cuts at:      0     821    1950 
   Size:        821    1129
 
XmnI GAAnn'nnTTC

Cuts at:      0     804    1607    1950 
   Size:        804     803     343

  Fragments arranged by size: 

                 804     803     343
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApoI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbsI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstYI
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI   HincII  HindIII     HpaI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NspI    PpuMI     PsiI
     PsrI     PstI     SbfI     ScaI     SfcI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BbeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsaAI    BseRI
    BseYI    BsiEI    BsiWI    BspHI    BsrFI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EagI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XcmI     XhoI     ZraI
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