################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeIII HgaI Hin1I HincII HindII HpaI HphI KasI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 42 CGCG CG^CG AciI 41 CCGC CCGC(-3/-1) AcsI 143 AAATTC [GA]^AATT[CT] AfaI 243 GTAC GT^AC ApoI 143 AAATTC [GA]^AATT[CT] AvaI 373 CTCGAG C^[CT]CG[GA]G BbvCI 51 CCTCAGC CCTCAGC(-5/-2) BbvI 338 GCTGC GCAGC(8/12) BcnI 182 CCCGG CC^[GC]GG BfaI 358 CTAG C^TAG BmyI 164 GTGCCC G[AGT]GC[ACT]^C BpmI 247 CTGGAG CTGGAG(16/14) BsaJI 8 CCAAGG C^C[ACGT][ACGT]GG BsoBI 373 CTCGAG C^[CT]CG[GA]G Bsp1286I 164 GTGCCC G[AGT]GC[ACT]^C BssSI 31 CTCGTG CACGAG(-5/-1) BstBI 103 TTCGAA TT^CGAA BstNI 246 CCTGG CC^[AT]GG BstUI 42 CGCG CG^CG BstXI 8 CCAAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 258 TTTAAA TTT^AAA EarI 328 GAAGAG CTCTTC(1/4) EcoRII 246 CCTGG ^CC[AT]GG EcoT14I 8 CCAAGG C^C[AT][AT]GG Fnu4HI 338 GCTGC GC^[ACGT]GC HaeII 403 GGCGCT [GA]GCGC^[CT] HapII 183 CCGG C^CGG HindIII 254 AAGCTT A^AGCTT HpaII 183 CCGG C^CGG ItaI 338 GCTGC GC^[ACGT]GC Ksp632I 328 GAAGAG CTCTTC(1/4) MaeI 358 CTAG C^TAG MaeII 128 ACGT A^CGT MseI 259 TTAA T^TAA MspI 183 CCGG C^CGG MvaI 246 CCTGG CC^[AT]GG MvnI 42 CGCG CG^CG NciI 182 CCCGG CC^[GC]GG NspV 103 TTCGAA TT^CGAA PaeR7I 373 CTCGAG C^TCGAG RsaI 243 GTAC GT^AC SfcI 81 CTACAG C^T[GA][CT]AG SfuI 103 TTCGAA TT^CGAA SmlI 373 CTCGAG C^T[CT][GA]AG SpeI 357 ACTAGT A^CTAGT StyI 8 CCAAGG C^C[AT][AT]GG Tru9I 259 TTAA T^TAA TseI 338 GCTGC G^C[AT]GC Tsp509I 144 AATT ^AATT XhoI 373 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 27 GGTCC G^G[AT]CC 381 GGACC BsiYI 342 CCCAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 372 CCTCGAGATGG BslI 342 CCCAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 372 CCTCGAGATGG BssKI 182 CCCGG ^CC[ACGT]GG 246 CCTGG Cfr13I 27 GGTCC G^G[ACGT]CC 381 GGACC DdeI 52 CTCAG C^T[ACGT]AG 76 CTGAG HinfI 98 GATTC G^A[ACGT]TC 234 GATTC NlaIII 92 CATG CATG^ 112 CATG Sau96I 27 GGTCC G^G[ACGT]CC 381 GGACC ScrFI 182 CCCGG CC^[ACGT]GG 246 CCTGG SfaNI 86 GATGC GCATC(5/9) 135 GCATC TaqI 104 TCGA T^CGA 374 TCGA TfiI 98 GATTC G^A[AT]TC 234 GATTC TthHB8I 104 TCGA T^CGA 374 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AluI CfoI HhaI HinP1I MboII MnlI MwoI