(Linear) (Six-base) MAPSORT of: KIBB9267.seq  Check: 427  from: 1  to: 411



KIBB9267.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     208     411 
   Size:        154      54     203

  Fragments arranged by size: 

                 203     154      54
 
ApoI r'AATT_y

Cuts at:      0     143     411 
   Size:        143     268
 
AvaI C'yCGr_G

Cuts at:      0     373     411 
   Size:        373      38
 
BbvCI CC'TCA_GC

Cuts at:      0      52     411 
   Size:         52     359
 
Bme1580I G_kGCm'C

Cuts at:      0     168     411 
   Size:        168     243
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     411 
   Size:        268     143
 
Bpu10I CC'TnA_GC

Cuts at:      0      52      76     411 
   Size:         52      24     335

  Fragments arranged by size: 

                 335      52      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     168     411 
   Size:        168     243
 
BssSI C'ACGA_G

Cuts at:      0      31     411 
   Size:         31     380
 
BstBI TT'CG_AA

Cuts at:      0     104     411 
   Size:        104     307
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      15     411 
   Size:         15     396
 
DraI TTT'AAA

Cuts at:      0     260     411 
   Size:        260     151
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     411 
   Size:        323      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     208     268     411 
   Size:        154      54      60     143

  Fragments arranged by size: 

                 154     143      60      54
 
HaeII r_GCGC'y

Cuts at:      0     407     411 
   Size:        407       4
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     411 
   Size:        113      32     266

  Fragments arranged by size: 

                 266     113      32
 
HindIII A'AGCT_T

Cuts at:      0     254     411 
   Size:        254     157
 
SfcI C'TryA_G

Cuts at:      0      81     411 
   Size:         81     330
 
SmlI C'TyrA_G

Cuts at:      0     373     411 
   Size:        373      38
 
SpeI A'CTAG_T

Cuts at:      0     357     411 
   Size:        357      54
 
StyI C'CwwG_G

Cuts at:      0       8     411 
   Size:          8     403
 
XhoI C'TCGA_G

Cuts at:      0     373     411 
   Size:        373      38
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BbvCI Bme1580I     BpmI   Bpu10I Bsp1286I
    BssSI    BstBI    BstXI     DraI     EarI  Eco57MI    HaeII    Hin4I
  HindIII     SfcI     SmlI     SpeI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI