Restriction map for pF1KB5682
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AocI        Aor51HI     AscI        
AseI        AsnI        Asp718I     AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BcgI        BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BssHII      BssSI       
BstBI       BstEII      BstPI       Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MamI        
MluI        MluNI       MroI        MscI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI          680  GTATAC               GT^[AC][GT]AC
AccII        1215  CGCG                 CG^CG
AcsI         1577  AAATTC               [GA]^AATT[CT]
AflIII        330  ACATGT               A^C[GA][CT]GT
AhdI            4  GACAATATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1259  GTGCAC               G^TGCAC
AlwI         1417  GGATC                GGATC(4/5)
ApaI         1592  GGGCCC               GGGCC^C
ApaLI        1259  GTGCAC               G^TGCAC
ApoI         1577  AAATTC               [GA]^AATT[CT]
Asp700I      1612  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI           4  GACAATATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         1199  GGTGCC               G^G[CT][GA]CC
BbvCI         730  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1054  GGATAC               GTATCC(6/5)
BglII        1283  AGATCT               A^GATCT
BsaAI        1057  TACGTG               [CT]AC^GT[GA]
BsaWI        1530  ACCGGA               [AT]^CCGG[AT]
BseRI        1073  GAGGAG               GAGGAG(10/8)
BsmBI         289  CGTCTC               CGTCTC(1/5)
BsmI          925  GCATTC               GAATGC(1/-1)
Bsp1407I     1224  TGTACA               T^GTACA
BspLU11I      330  ACATGT               A^CATGT
BsrDI         975  CATTGC               GCAATG(2/0)
BsrGI        1224  TGTACA               T^GTACA
Bst1107I      680  GTATAC               GTA^TAC
BstUI        1215  CGCG                 CG^CG
BstXI         677  CCAGTATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1283  AGATCT               [GA]^GATC[CT]
BstZ17I       680  GTATAC               GTA^TAC
DrdI         1210  GACCACGCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1391  CCGTGG               C^C[GA][CT]GG
EaeI          855  CGGCCA               [CT]^GGCC[GA]
Eam1105I        4  GACAATATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      1494  ATGCAT               ATGCA^T
FauI          873  CCCGC                CCCGC(4/6)
HgaI         1320  GCGTC                GACGC(5/10)
HincII       1094  GTCAAC               GT[CT]^[GA]AC
HindII       1094  GTCAAC               GT[CT]^[GA]AC
MaeII        1058  ACGT                 A^CGT
MfeI          116  CAATTG               C^AATTG
MflI         1283  AGATCT               [GA]^GATC[CT]
MunI          116  CAATTG               C^AATTG
MvnI         1215  CGCG                 CG^CG
NsiI         1494  ATGCAT               ATGCA^T
NspI          330  ACATGT               [GA]CATG^[CT]
PleI          613  GAGTC                GAGTC(4/5)
PshAI        1480  GACCTTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          194  GAGCTC               GAGCT^C
ScaI         1614  AGTACT               AGT^ACT
SspBI        1224  TGTACA               T^GTACA
SspI          338  AATATT               AAT^ATT
Tsp45I       1208  GTGAC                ^GT[GC]AC
XhoII        1283  AGATCT               [GA]^GATC[CT]
XmnI         1612  GAAGTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI         1225  GTAC                 GT^AC
             1615  GTAC                
AlwNI        1370  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1454  CAGCTGCTG           
AspHI         194  GAGCTC               G[AT]GC[AT]^C
             1259  GTGCAC              
AvaII        1062  GGACC                G^G[AT]CC
             1528  GGACC               
BanII         194  GAGCTC               G[GA]GC[CT]^C
             1592  GGGCCC              
BcnI          892  CCCGG                CC^[GC]GG
             1590  CCGGG               
BfaI          203  CTAG                 C^TAG
             1606  CTAG                
BsiHKAI       194  GAGCTC               G[AT]GC[AT]^C
             1259  GTGCAC              
BsmFI         501  GGGAC                GGGAC(10/14)
             1527  GGGAC               
BstAPI       1360  GCACAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1369  GCAGCAGCTGC         
DraII         726  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1592  GGGCCCT             
EcoO109I      726  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1592  GGGCCCT             
MaeI          203  CTAG                 C^TAG
             1606  CTAG                
MboII         309  TCTTC                GAAGA(8/7)
              861  TCTTC               
MslI          313  CACAAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              648  CATTCCTGTG          
MspA1I       1373  CAGCTG               C[AC]G^C[GT]G
             1454  CAGCTG              
NciI          892  CCCGG                CC^[GC]GG
             1590  CCGGG               
PstI          442  CTGCAG               CTGCA^G
             1367  CTGCAG              
PvuII        1373  CAGCTG               CAG^CTG
             1454  CAGCTG              
RsaI         1225  GTAC                 GT^AC
             1615  GTAC                
SfcI          442  CTGCAG               C^T[GA][CT]AG
             1367  CTGCAG              
TaqI          571  TCGA                 T^CGA
             1332  TCGA                
TfiI          163  GATTC                G^A[AT]TC
              384  GAATC               
TthHB8I       571  TCGA                 T^CGA
             1332  TCGA                
XcmI           75  CCATAGACAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              644  CCAACATTCCTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        BbvI        BmyI        BpmI        
BsaJI       BsgI        BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoNI       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       Tru9I       TseI        
Tsp509I     TspRI       
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