Restriction map for pF1KB5682
 (Linear) (Six-base) MAPSORT of: KIBB5682.seq  Check: 777  from: 1  to: 1656



KIBB5682.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AccI GT'mk_AC

Cuts at:      0     681    1656 
   Size:        681     975
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     229     542     847    1656 
   Size:        160      69     313     305     809

  Fragments arranged by size: 

                 809     313     305     160      69
 
AflIII A'CryG_T

Cuts at:      0     330    1656 
   Size:        330    1326
 
AhdI GACnn_n'nnGTC

Cuts at:      0       9    1656 
   Size:          9    1647
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     467    1174    1656 
   Size:        467     707     482

  Fragments arranged by size: 

                 707     482     467
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1259    1291    1656 
   Size:       1259      32     365

  Fragments arranged by size: 

                1259     365      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1375    1459    1656 
   Size:       1375      84     197

  Fragments arranged by size: 

                1375     197      84
 
ApaI G_GGCC'C

Cuts at:      0    1596    1656 
   Size:       1596      60
 
ApaLI G'TGCA_C

Cuts at:      0    1259    1656 
   Size:       1259     397
 
ApoI r'AATT_y

Cuts at:      0    1577    1656 
   Size:       1577      79
 
BanI G'GyrC_C

Cuts at:      0    1199    1656 
   Size:       1199     457
 
BanII G_rGCy'C

Cuts at:      0     198    1596    1656 
   Size:        198    1398      60

  Fragments arranged by size: 

                1398     198      60
 
BbvCI CC'TCA_GC

Cuts at:      0     731    1656 
   Size:        731     925
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1048    1656 
   Size:       1048     608
 
BfrBI ATG'CAT

Cuts at:      0    1496    1656 
   Size:       1496     160
 
BglII A'GATC_T

Cuts at:      0    1283    1656 
   Size:       1283     373
 
Bme1580I G_kGCm'C

Cuts at:      0    1263    1596    1656 
   Size:       1263     333      60

  Fragments arranged by size: 

                1263     333      60
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     694    1656 
   Size:        694     962
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     470    1656 
   Size:        470    1186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315    1091    1286    1421    1582    1656 
   Size:        315     776     195     135     161      74

  Fragments arranged by size: 

                 776     315     195     161     135      74
 
Bpu10I CC'TnA_GC

Cuts at:      0     731    1656 
   Size:        731     925
 
BsaAI yAC'GTr

Cuts at:      0    1059    1656 
   Size:       1059     597
 
BsaWI w'CCGG_w

Cuts at:      0    1530    1656 
   Size:       1530     126
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1259    1289    1656 
   Size:       1259      30     367

  Fragments arranged by size: 

                1259     367      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1088    1656 
   Size:       1088     568
 
BseYI C'CCAG_C

Cuts at:      0     591    1252    1523    1656 
   Size:        591     661     271     133

  Fragments arranged by size: 

                 661     591     271     133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1180    1343    1656 
   Size:        803     377     163     313

  Fragments arranged by size: 

                 803     377     313     163
 
BsiHKAI G_wGCw'C

Cuts at:      0     198    1263    1656 
   Size:        198    1065     393

  Fragments arranged by size: 

                1065     393     198
 
BsmI GAATG_Cn'

Cuts at:      0     925    1656 
   Size:        925     731
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     295    1656 
   Size:        295    1361
 
Bsp1286I G_dGCh'C

Cuts at:      0     198    1263    1596    1656 
   Size:        198    1065     333      60

  Fragments arranged by size: 

                1065     333     198      60
 
BsrDI GCAATG_nn'

Cuts at:      0     974    1656 
   Size:        974     682
 
BsrGI T'GTAC_A

Cuts at:      0    1224    1656 
   Size:       1224     432
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1366    1375    1656 
   Size:       1366       9     281

  Fragments arranged by size: 

                1366     281       9
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     684    1656 
   Size:        684     972
 
BstYI r'GATC_y

Cuts at:      0    1283    1656 
   Size:       1283     373
 
BstZ17I GTA'TAC

Cuts at:      0     682    1656 
   Size:        682     974
 
BtgI C'CryG_G

Cuts at:      0    1391    1656 
   Size:       1391     265
 
BtsI GCAGTG_nn'

Cuts at:      0     439     629    1656 
   Size:        439     190    1027

  Fragments arranged by size: 

                1027     439     190
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1216    1656 
   Size:       1216     440
 
EaeI y'GGCC_r

Cuts at:      0     855    1656 
   Size:        855     801
 
EcoICRI GAG'CTC

Cuts at:      0     196    1656 
   Size:        196    1460
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     229     315     542     847    1091    1286    1421 
   Size:        160      69      86     227     305     244     195     135

Cuts at:   1421    1582    1656 
   Size:        161      74

  Fragments arranged by size: 

                 305     244     227     195     161     160     135      86
                  74      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     734    1070    1412    1656 
   Size:        734     336     342     244

  Fragments arranged by size: 

                 734     342     336     244
 
EcoO109I rG'GnC_Cy

Cuts at:      0     727    1593    1656 
   Size:        727     866      63

  Fragments arranged by size: 

                 866     727      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27    1535    1567    1656 
   Size:         27    1508      32      89

  Fragments arranged by size: 

                1508      89      32      27
 
HincII GTy'rAC

Cuts at:      0    1096    1656 
   Size:       1096     560
 
MfeI C'AATT_G

Cuts at:      0     116    1656 
   Size:        116    1540
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     238    1145    1656 
   Size:        238     907     511

  Fragments arranged by size: 

                 907     511     238
 
MslI CAynn'nnrTG

Cuts at:      0     317     652    1656 
   Size:        317     335    1004

  Fragments arranged by size: 

                1004     335     317
 
MspA1I CmG'CkG

Cuts at:      0    1375    1456    1656 
   Size:       1375      81     200

  Fragments arranged by size: 

                1375     200      81
 
NsiI A_TGCA'T

Cuts at:      0    1498    1656 
   Size:       1498     158
 
NspI r_CATG'y

Cuts at:      0     334    1656 
   Size:        334    1322
 
PciI A'CATG_T

Cuts at:      0     330    1656 
   Size:        330    1326
 
PfoI T'CCnGG_A

Cuts at:      0     891    1656 
   Size:        891     765
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1259    1291    1656 
   Size:       1259      32     365

  Fragments arranged by size: 

                1259     365      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1484    1656 
   Size:       1484     172
 
PspOMI G'GGCC_C

Cuts at:      0    1592    1656 
   Size:       1592      64
 
PstI C_TGCA'G

Cuts at:      0     446    1371    1656 
   Size:        446     925     285

  Fragments arranged by size: 

                 925     446     285
 
PvuII CAG'CTG

Cuts at:      0    1375    1456    1656 
   Size:       1375      81     200

  Fragments arranged by size: 

                1375     200      81
 
SacI G_AGCT'C

Cuts at:      0     198    1656 
   Size:        198    1458
 
ScaI AGT'ACT

Cuts at:      0    1616    1656 
   Size:       1616      40
 
SfcI C'TryA_G

Cuts at:      0     442    1367    1656 
   Size:        442     925     289

  Fragments arranged by size: 

                 925     442     289
 
SspI AAT'ATT

Cuts at:      0     340    1656 
   Size:        340    1316
 
TatI w'GTAC_w

Cuts at:      0    1224    1614    1656 
   Size:       1224     390      42

  Fragments arranged by size: 

                1224     390      42
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      82     651    1656 
   Size:         82     569    1005

  Fragments arranged by size: 

                1005     569      82
 
XmnI GAAnn'nnTTC

Cuts at:      0    1616    1656 
   Size:       1616      40
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     BanI    BanII    BbvCI    BciVI    BfrBI    BglII
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrDI
    BsrGI   BstAPI    BstXI    BstYI  BstZ17I     BtgI     BtsI     DrdI
     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MfeI
     MmeI     MslI   MspA1I     NsiI     NspI     PciI     PfoI     PpiI
    PshAI   PspOMI     PstI    PvuII     SacI     ScaI     SfcI     SspI
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI     BcgI     BclI     BglI     BlpI    BmgBI     BmtI    BpuEI
     BsaI    BsaBI    BsaHI    BsiEI    BsiWI    BspEI    BspHI    BspMI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI     PmeI
     PmlI    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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