Restriction map for pF1KB5670
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccII       AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BciVI       BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaHI       
BsaI        BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     FauI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       Sse8387I    
StuI        Tsp45I      Tth111I     Van91I      XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2531  GTAGAC               GT^[AC][GT]AC
AccIII         47  TCCGGA               T^CCGGA
AciI         1635  GCGG                 CCGC(-3/-1)
AclI          323  AACGTT               AA^CGTT
AflIII       2261  ACATGT               A^C[GA][CT]GT
ApaI          144  GGGCCC               GGGCC^C
AseI          394  ATTAAT               AT^TAAT
AsnI          394  ATTAAT               AT^TAAT
AvaII          60  GGACC                G^G[AT]CC
BamHI        1806  GGATCC               G^GATCC
BclI         1154  TGATCA               T^GATCA
BcnI         2025  CCCGG                CC^[GC]GG
BlpI         1426  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1426  GCTCAGC              GC^T[ACGT]AGC
BsaWI          47  TCCGGA               [AT]^CCGG[AT]
BseAI          47  TCCGGA               T^CCGGA
BseRI         301  GAGGAG               GAGGAG(10/8)
BsmAI        1879  GAGAC                GTCTC(1/5)
BspEI          47  TCCGGA               T^CCGGA
BspHI        2355  TCATGA               T^CATGA
BspLU11I     2261  ACATGT               A^CATGT
BstAPI       2429  GCAGAAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1209  CCAGGTCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1426  GCTCAGC              GC^T[ACGT]AGC
DraII         143  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DsaI          210  CCGTGG               C^C[GA][CT]GG
EcoO109I      143  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         672  GAATTC               G^AATTC
EcoRV         364  GATATC               GAT^ATC
FbaI         1154  TGATCA               T^GATCA
MroI           47  TCCGGA               T^CCGGA
NciI         2025  CCCGG                CC^[GC]GG
PacI          391  TTAATTAA             TTAAT^TAA
PshBI         394  ATTAAT               AT^TAAT
Psp1406I      323  AACGTT               AA^CGTT
RcaI         2355  TCATGA               T^CATGA
SacI          187  GAGCTC               GAGCT^C
SapI         2192  GAAGAGC              GCTCTTC(1/4)
SspI         2074  AATATT               AAT^ATT
SwaI         1410  ATTTAAAT             ATTT^AAAT
XbaI         1170  TCTAGA               T^CTAGA
XcmI         1907  CCAGAGCACTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI        2093  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2704  CAGACACTG           
BanII         144  GGGCCC               G[GA]GC[CT]^C
              187  GAGCTC              
BbsI           51  GAAGAC               GAAGAC(2/6)
             1834  GAAGAC              
BcgI         2596  CGACAGAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1469  GCAAAGACCTCG        
BglII         594  AGATCT               A^GATCT
             1752  AGATCT              
BpuAI          51  GAAGAC               GAAGAC(2/6)
             1834  GAAGAC              
BsgI          112  GTGCAG               GTGCAG(16/14)
              452  GTGCAG              
Bsp1407I      710  TGTACA               T^GTACA
             2264  TGTACA              
BspMI         238  GCAGGT               ACCTGC(4/8)
              448  GCAGGT              
BsrGI         710  TGTACA               T^GTACA
             2264  TGTACA              
EcoNI          92  CCTGGTTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1062  CCTTAAAAAGG         
EcoT14I      1268  CCTTGG               C^C[AT][AT]GG
             2444  CCTTGG              
EcoT22I      1557  ATGCAT               ATGCA^T
             2048  ATGCAT              
HapII          48  CCGG                 C^CGG
             2026  CCGG                
HpaII          48  CCGG                 C^CGG
             2026  CCGG                
MspI           48  CCGG                 C^CGG
             2026  CCGG                
NsiI         1557  ATGCAT               ATGCA^T
             2048  ATGCAT              
SmlI          172  CTCAAG               C^T[CT][GA]AG
              598  CTTGAG              
SpeI         1129  ACTAGT               A^CTAGT
             2224  ACTAGT              
SphI         1555  GCATGC               GCATG^C
             1668  GCATGC              
SspBI         710  TGTACA               T^GTACA
             2264  TGTACA              
StyI         1268  CCTTGG               C^C[AT][AT]GG
             2444  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AspHI       BbvI        BfaI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       BsmI        Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HgaI        
HinfI       ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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