Restriction map for
pF1KB5663
(Linear) (Six-base) MAPSORT of: KIBB5663.seq Check: 7642 from: 1 to: 885
KIBB5663.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 885
Size: 115 770
AleI CACnn'nnGTG
Cuts at: 0 574 865 885
Size: 574 291 20
Fragments arranged by size:
574 291 20
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 885
Size: 28 857
AlwNI CAG_nnn'CTG
Cuts at: 0 15 303 885
Size: 15 288 582
Fragments arranged by size:
582 288 15
AvaI C'yCGr_G
Cuts at: 0 400 885
Size: 400 485
BanII G_rGCy'C
Cuts at: 0 278 658 885
Size: 278 380 227
Fragments arranged by size:
380 278 227
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 885
Size: 222 663
BclI T'GATC_A
Cuts at: 0 707 885
Size: 707 178
BmgBI CAC'GTC
Cuts at: 0 867 876 885
Size: 867 9 9
Fragments arranged by size:
867 9 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 292 684 784 885
Size: 214 78 392 100 101
Fragments arranged by size:
392 214 101 100 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 885
Size: 427 458
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 337 885
Size: 100 237 548
Fragments arranged by size:
548 237 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 478 508 885
Size: 26 452 30 377
Fragments arranged by size:
452 377 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 118 235 238 418 529 666 829
Size: 55 63 117 3 180 111 137 163
Cuts at: 829 832 841 844 847 850 885
Size: 3 9 3 3 3 35
Fragments arranged by size:
180 163 137 117 111 63 55 35
9 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 303 785 885
Size: 303 482 100
Fragments arranged by size:
482 303 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 885
Size: 163 722
BsiEI CG_ry'CG
Cuts at: 0 75 362 885
Size: 75 287 523
Fragments arranged by size:
523 287 75
Bsp1286I G_dGCh'C
Cuts at: 0 278 658 885
Size: 278 380 227
Fragments arranged by size:
380 278 227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 118 885
Size: 118 767
BsrDI GCAATG_nn'
Cuts at: 0 467 885
Size: 467 418
BssHII G'CGCG_C
Cuts at: 0 113 885
Size: 113 772
Bsu36I CC'TnA_GG
Cuts at: 0 649 885
Size: 649 236
BtgI C'CryG_G
Cuts at: 0 602 885
Size: 602 283
BtsI GCAGTG_nn'
Cuts at: 0 638 885
Size: 638 247
EaeI y'GGCC_r
Cuts at: 0 239 359 455 626 885
Size: 239 120 96 171 259
Fragments arranged by size:
259 239 171 120 96
EagI C'GGCC_G
Cuts at: 0 359 885
Size: 359 526
EarI CTCTTCn'nnn_
Cuts at: 0 818 885
Size: 818 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 115 526 584 885
Size: 28 87 411 58 301
Fragments arranged by size:
411 301 87 58 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 214 292 684 784 885
Size: 115 99 78 392 100 101
Fragments arranged by size:
392 115 101 100 99 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 778 885
Size: 778 107
EcoO109I rG'GnC_Cy
Cuts at: 0 153 885
Size: 153 732
MscI TGG'CCA
Cuts at: 0 241 885
Size: 241 644
MslI CAynn'nnrTG
Cuts at: 0 187 316 322 574 865 885
Size: 187 129 6 252 291 20
Fragments arranged by size:
291 252 187 129 20 6
MspA1I CmG'CkG
Cuts at: 0 237 300 613 885
Size: 237 63 313 272
Fragments arranged by size:
313 272 237 63
NcoI C'CATG_G
Cuts at: 0 602 885
Size: 602 283
NspI r_CATG'y
Cuts at: 0 321 885
Size: 321 564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 28 476 508 885
Size: 28 448 32 377
Fragments arranged by size:
448 377 32 28
PpuMI rG'GwC_Cy
Cuts at: 0 153 885
Size: 153 732
PstI C_TGCA'G
Cuts at: 0 299 885
Size: 299 586
PvuII CAG'CTG
Cuts at: 0 300 885
Size: 300 585
SfcI C'TryA_G
Cuts at: 0 295 885
Size: 295 590
SmlI C'TyrA_G
Cuts at: 0 442 885
Size: 442 443
StuI AGG'CCT
Cuts at: 0 641 885
Size: 641 244
StyI C'CwwG_G
Cuts at: 0 602 803 885
Size: 602 201 82
Fragments arranged by size:
602 201 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 873 885
Size: 873 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 478 510 885
Size: 199 32 247 32 375
Fragments arranged by size:
375 247 199 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 249 885
Size: 189 60 636
Fragments arranged by size:
636 189 60
Enzymes that do cut:
AcuI AleI AloI AlwNI AvaI BanII BbsI BclI
BmgBI BpmI BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiEI Bsp1286I BspMI BsrDI BssHII Bsu36I BtgI BtsI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I MscI
MslI MspA1I NcoI NspI PpiI PpuMI PstI PvuII
SfcI SmlI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI